pCMV-3×FLAG-DUSP3-Neo plasmid

pCMV-3×FLAG-DUSP3-Neo plasmid

PVT93928

pCMV-3×FLAG-DUSP3-Neo plasmid Information

Plasmid name: pCMV-3×FLAG-DUSP3(human)-Neo
Alias: NM_004090.4;VHR
Gene lenght: 558bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: T7
Reverse primer:
Copy number: High
Induction:

pCMV-3×FLAG-DUSP3-Neo plasmid Description

pCMV-3×FLAG-DUSP3-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-3×FLAG-DUSP3-Neo plasmid Sequence

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagccaccat ggactacaaa gaccatgacg gtgattataa agatcatgac 961 atcgactaca aggatgacga tgacaagctt atgtcgggct cgttcgagct ctcggtgcag 1021 gatctcaacg acctgctctc ggacggcagc ggctgctaca gcctcccgag ccagccctgc 1081 aacgaggtca ccccgcggat ctacgtgggc aacgcgtctg tggctcagga catccccaag 1141 ctgcagaaac taggcatcac ccatgtgctg aacgcggctg agggcaggtc cttcatgcac 1201 gtcaacacca atgccaactt ctacaaggac tccggcatca catacctggg catcaaggcc 1261 aacgacacac aggagttcaa cctcagcgct tactttgaaa gggctgccga cttcattgac 1321 caggctttgg ctcaaaagaa tggccgggtg ctcgtccact gccgggaagg ttatagccgc 1381 tccccaacgc tagttatcgc ctacctcatg atgcggcaga agatggacgt caagtctgcc 1441 ctgagcatcg tgaggcagaa ccgtgagatc ggccccaacg atggcttcct ggcccagctc 1501 tgccagctca atgacagact agccaaggag gggaagttga aaccctagga attctgcaga 1561 tatccagcac agtggcggcc gctcgagtct agagggcccg tttaaacccg ctgatcagcc 1621 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg 1681 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat 1741 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag 1801 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg 1861 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc 1921 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc 1981 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct 2041 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa 2101 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc 2161 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca 2221 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat 2281 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt 2341 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc 2401 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta 2461 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc 2521 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta 2581 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct 2641 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat 2701 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag 2761 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg 2821 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca 2881 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc 2941 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg 3001 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg 3061 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta 3121 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag 3181 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac 3241 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg 3301 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg 3361 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg 3421 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg 3481 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg 3541 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc 3601 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct 3661 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta 3721 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 3781 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc 3841 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta 3901 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc 3961 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg 4021 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg 4081 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 4141 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 4201 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 4261 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 4321 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 4381 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 4441 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 4501 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 4561 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 4621 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 4681 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct 4741 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 4801 tggtagcggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 4861 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 4921 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 4981 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 5041 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 5101 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 5161 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 5221 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 5281 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 5341 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 5401 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 5461 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 5521 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 5581 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 5641 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 5701 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 5761 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 5821 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 5881 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 5941 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 6001 tccccgaaaa gtgccacctg acgtc

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.