pLV3-CMV-NDC80-3×HA-CopGFP-Pur

pLV3-CMV-NDC80-3×HA-CopGFP-Puro plasmid

PVT96875

pLV3-CMV-NDC80-3×HA-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-NDC80(human)-3×HA-CopGFP-Puro
Alias: NM_006101.3;BC035617.1;HEC; HEC1; HsHec1; hsNDC80; KNTC2; TID3
Gene lenght: 1926bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-NDC80-3×HA-CopGFP-Puro plasmid Description

pLV3-CMV-NDC80-3×HA-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-NDC80-3×HA-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatga agcgcagttc agtttccagc 2341 ggtggtgctg gccgcctctc catgcaggag ttaagatccc aggatgtaaa taaacaaggc 2401 ctctataccc ctcaaaccaa agagaaacca acctttggaa agttgagtat aaacaaaccg 2461 acatctgaaa gaaaagtctc gctatttggc aaaagaacta gtggacatgg atcccggaat 2521 agtcaacttg gtatattttc cagttctgag aaaatcaagg acccgagacc acttaatgac 2581 aaagcattca ttcagcagtg tattcgacaa ctctgtgagt ttcttacaga aaatggttat 2641 gcacataatg tgtccatgaa atctctacaa gctccctctg ttaaagactt cctgaagatc 2701 ttcacatttc tttatggctt cctgtgcccc tcatacgaac ttcctgacac aaagtttgaa 2761 gaagaggttc caagaatctt taaagacctt gggtatcctt ttgcactatc caaaagctcc 2821 atgtacacag tgggggctcc tcatacatgg cctcacattg tggcagcctt agtttggcta 2881 atagactgca tcaagataca tactgccatg aaagaaagct cacctttatt tgatgatggg 2941 cagccttggg gagaagaaac tgaagatgga attatgcata ataagttgtt tttggactac 3001 accataaaat gctatgagag ttttatgagt ggtgccgaca gctttgatga gatgaatgca 3061 gagctgcagt caaaactgaa ggatttattt aatgtggatg cttttaagct ggaatcatta 3121 gaagcaaaaa acagagcatt gaatgaacag attgcaagat tggaacaaga aagagaaaaa 3181 gaaccgaatc gtctagagtc gttgagaaaa ctgaaggctt ccttacaagg agatgttcaa 3241 aagtatcagg catacatgag caatttggag tctcattcag ccattcttga ccagaaatta 3301 aatggtctca atgaggaaat tgctagagta gaactagaat gtgaaacaat aaaacaggag 3361 aacactcgac tacagaatat cattgacaac cagaagtact cagttgcaga cattgagcga 3421 ataaatcatg aaagaaatga attgcagcag actattaata aattaaccaa ggacctggaa 3481 gctgaacaac agaagttgtg gaatgaggag ttaaaatatg ccagaggcaa agaagcgatt 3541 gaaacacaat tagcagagta tcacaaattg gctagaaaat taaaacttat tcctaaaggt 3601 gctgagaatt ccaaaggtta tgactttgaa attaagttta atcccgaggc tggtgccaac 3661 tgccttgtca aatacagggc tcaagtttat gtacctctta aggaactcct gaatgaaact 3721 gaagaagaaa ttaataaagc cctaaataaa aaaatgggtt tggaggatac tttagaacaa 3781 ttgaatgcaa tgataacaga aagcaagaga agtgtgagaa ctctgaaaga agaagttcaa 3841 aagctggatg atctttacca acaaaaaatt aaggaagcag aggaagagga tgaaaaatgt 3901 gccagtgagc ttgagtcctt ggagaaacac aagcacctgc tagaaagtac tgttaaccag 3961 gggctcagtg aagctatgaa tgaattagat gctgttcagc gggaatacca actagttgtg 4021 caaaccacga ctgaagaaag acgaaaagtg ggaaataact tgcaacgtct gttagagatg 4081 gttgctacac atgttgggtc tgtagagaaa catcttgagg agcagattgc taaagttgat 4141 agagaatatg aagaatgcat gtcagaagat ctctcggaaa atattaaaga gattagagat 4201 aagtatgaga agaaagctac tctaattaag tcttctgaag aaCTCGAGTA CCCATACGAC 4261 GTACCTGATT ATGCAACTTA TCCTTATGAT GTACCTGATT ATGCTAGCTA TCCTTATGAT 4321 GTCCCAGACT ACGCATAGgg atccCTGCGA TATCCAGCgc ggccgcGaag gatctgcgat 4381 cgctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg 4441 ggaggggtcg gcaattgaac gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt 4501 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca 4561 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca gctgaagctt 4621 cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc 4681 cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc 4741 taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta 4801 cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt 4861 gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc tacgctagac 4921 gccaccatgg agagcgacga gagcggcctg cccgccatgg agatcgagtg ccgcatcacc 4981 ggcaccctga acggcgtgga gttcgagctg gtgggcggcg gagagggcac ccccaagcag 5041 ggccgcatga ccaacaagat gaagagcacc aaaggcgccc tgaccttcag cccctacctg 5101 ctgagccacg tgatgggcta cggcttctac cacttcggca cctaccccag cggctacgag 5161 aaccccttcc tgcacgccat caacaacggc ggctacacca acacccgcat cgagaagtac 5221 gaggacggcg gcgtgctgca cgtgagcttc agctaccgct acgaggccgg ccgcgtgatc 5281 ggcgacttca aggtggtggg caccggcttc cccgaggaca gcgtgatctt caccgacaag 5341 atcatccgca gcaacgccac cgtggagcac ctgcacccca tgggcgataa cgtgctggtg 5401 ggcagcttcg cccgcacctt cagcctgcgc gacggcggct actacagctt cgtggtggac 5461 agccacatgc acttcaagag cgccatccac cccagcatcc tgcagaacgg gggccccatg 5521 ttcgccttcc gccgcgtgga ggagctgcac agcaacaccg agctgggcat cgtggagtac 5581 cagcacgcct tcaagacccc catcgccttc gccagatccc gcgctcagtc gtccaattct 5641 gccgtggacg gcaccgccgg acccggctcc accggatctc gcgagggcag aggaagtctt 5701 ctaacatgcg gtgacgtgga ggagaatccc ggccctatga ccgagtacaa gcccacggtg 5761 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc 5821 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag 5881 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac 5941 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc 6001 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag 6061 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc 6121 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg 6181 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc 6241 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg 6301 cgcacctggt gcatgacccg caagcccggt gcctgaaatc aacctctgga ttacaaaatt 6361 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct 6421 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg 6481 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc 6541 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt 6601 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc 6661 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg 6721 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg 6781 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc 6841 ggcctgctgc cggctctgcg gcctcttccg cgtctccgcc ttcgccctca gacgagtcgg 6901 atctcccttt ggccgcctcc ccgcctggta cctttaagac caatgactta caaggcagct 6961 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa 7021 cgaaaataag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc 7081 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga 7141 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga 7201 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag 7261 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct 7321 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca 7381 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag 7441 ctatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt 7501 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag 7561 gaggcttttt tggaggccta gacttttgca gagacggccc aaattcgtaa tcatggtcat 7621 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa 7681 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc 7741 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc 7801 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact 7861 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac 7921 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 7981 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 8041 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 8101 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 8161 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac 8221 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 8281 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 8341 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 8401 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga 8461 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 8521 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 8581 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 8641 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct 8701 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt 8761 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc 8821 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg 8881 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag 8941 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt 9001 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag 9061 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt 9121 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca 9181 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg 9241 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat 9301 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta 9361 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca 9421 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct 9481 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat 9541 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa 9601 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt 9661 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa 9721 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa 9781 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg 9841 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag 9901 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg 9961 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc 10021 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt 10081 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac 10141 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt 10201 cccagtcacg acgttgtaaa acgacggcca gtgccaagct g

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.