pTac-GST-TOP1MT plasmid

pTac-GST-TOP1MT plasmid

PVT95904

pTac-GST-TOP1MT plasmid Information

Plasmid name: pTac-GST-TOP1MT(human)
Alias: NM_052963.3;TOP1MT
Gene lenght: 1806bp
Host: E.coli
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: Tac
Replicon: pBR322
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: GST-F2
Reverse primer:
Copy number: Low
Induction: IPTG Induction

pTac-GST-TOP1MT plasmid Description

pTac-GST-TOP1MT is a plasmid suitable for protein expression in Escherichia coli.

pTac-GST-TOP1MT plasmid Sequence

1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg 61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt 121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc 181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca 241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc 301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc 361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc 421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata 481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc 541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact 601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag 661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt 721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa 781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat 841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc 901 atcctccaaa atcggatctg gttccgcgtg gatccATGCG CGTGGTGCGG CTGCTGCGGC 961 TCCGGGCGGC TCTGACGCTG CTCGGGGAGG TCCCCCGCCG CCCGGCCTCC CGGGGTGTCC 1021 CGGGCTCGCG CAGGACGCAG AAGGGCAGTG GAGCCAGGTG GGAGAAGGAG AAGCACGAAG 1081 ACGGGGTGAA GTGGAGACAG CTGGAGCACA AGGGCCCGTA CTTCGCACCC CCATACGAGC 1141 CCCTTCCCGA CGGAGTGCGT TTCTTCTATG AAGGAAGGCC TGTGAGATTG AGCGTGGCAG 1201 CGGAGGAGGT CGCCACTTTT TATGGGAGGA TGTTAGATCA TGAATACACA ACAAAGGAGG 1261 TTTTCCGGAA GAACTTCTTC AATGACTGGC GAAAGGAAAT GGCGGTGGAA GAGAGGGAAG 1321 TCATCAAGAG CCTGGACAAG TGTGACTTCA CGGAGATCCA CAGATACTTT GTGGACAAGG 1381 CCGCAGCCCG GAAAGTCCTG AGCAGGGAGG AGAAGCAGAA GCTAAAAGAA GAGGCAGAAA 1441 AACTTCAGCA AGAGTTCGGC TACTGTATTT TAGATGGTCA CCAAGAAAAA ATAGGCAACT 1501 TCAAGATTGA GCCGCCTGGC TTGTTCCGTG GCCGTGGCGA CCATCCCAAG ATGGGGATGC 1561 TGAAGAGAAG GATCACGCCA GAGGATGTGG TTATCAACTG CAGCAGGGAC TCGAAGATCC 1621 CCGAGCCGCC GGCGGGGCAC CAGTGGAAGG AGGTGCGCTC CGATAACACC GTCACGTGGC 1681 TGGCAGCTTG GACCGAGAGC GTTCAGAACT CCATCAAGTA CATCATGCTG AACCCTTGCT 1741 CGAAGCTGAA GGGGGAGACA GCTTGGCAGA AGTTTGAAAC AGCTCGACGC CTGCGGGGAT 1801 TTGTGGACGA GATCCGCTCC CAGTACCGGG CTGACTGGAA GTCTCGGGAA ATGAAGACGA 1861 GACAGCGGGC GGTGGCCCTG TATTTCATCG ATAAGCTGGC ACTGAGAGCA GGAAATGAGA 1921 AGGAGGACGG TGAGGCGGCC GACACCGTGG GCTGCTGTTC CCTCCGCGTG GAGCACGTCC 1981 AGCTGCACCC GGAGGCCGAT GGCTGCCAAC ACGTGGTGGA ATTTGACTTC CTGGGGAAGG 2041 ACTGCATCCG CTACTACAAC AGAGTGCCGG TGGAGAAGCC GGTGTACAAG AACTTACAGC 2101 TCTTTATGGA GAACAAGGAC CCCCGGGACG ACCTCTTCGA CAGGCTGACC ACGACCAGCC 2161 TGAACAAGCA CCTCCAGGAG CTGATGGACG GGCTGACGGC CAAGGTGTTC CGGACCTACA 2221 ACGCCTCCAT CACTCTGCAG GAGCAGCTGC GGGCCCTGAC GCGCGCCGAG GACAGCATAG 2281 CAGCTAAGAT CTTATCCTAC AACCGAGCCA ACCGAGTCGT GGCCATTCTC TGCAACCATC 2341 AGCGAGCAAC CCCCAGTACG TTCGAGAAGT CGATGCAGAA TCTCCAGACG AAGATCCAGG 2401 CAAAGAAGGA GCAGGTGGCT GAGGCCAGGG CAGAGCTGAG GAGGGCGAGG GCTGAGCACA 2461 AAGCCCAAGG GGATGGCAAG TCCAGGAGTG TCCTGGAGAA GAAGAGGCGG CTCCTGGAGA 2521 AGCTGCAGGA GCAGCTGGCG CAGCTGAGTG TGCAGGCCAC GGACAAGGAG GAGAACAAGC 2581 AGGTGGCCCT GGGCACGTCC AAGCTCAACT ACCTGGACCC CAGGATCAGC ATTGCCTGGT 2641 GCAAGCGGTT CAGGGTGCCA GTGGAGAAGA TCTACAGCAA AACACAGCGG GAGAGGTTCG 2701 CCTGGGCTCT CGCCATGGCA GGAGAAGACT TTGAATTCTA Actcgagcgg ccgcatcgtg 2761 actgactgac gatctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc 2821 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc 2881 agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg 2941 atagcggagt gtataattct tgaagacgaa agggcctcgt gatacgccta tttttatagg 3001 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc 3061 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac 3121 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt 3181 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag 3241 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg 3301 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa 3361 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc 3421 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag 3481 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa 3541 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc 3601 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg 3661 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa 3721 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa 3781 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg 3841 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag 3901 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg 3961 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt 4021 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt 4081 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac 4141 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag 4201 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg 4261 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca 4321 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga 4381 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca 4441 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc 4501 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca 4561 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa 4621 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc 4681 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc 4741 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg 4801 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat 4861 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca 4921 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt 4981 attttctcct tacgcatctg tgcggtattt cacaccgcat aaattccgac accatcgaat 5041 ggtgcaaaac ctttcgcggt atggcatgat agcgcccgga agagagtcaa ttcagggtgg 5101 tgaatgtgaa accagtaacg ttatacgatg tcgcagagta tgccggtgtc tcttatcaga 5161 ccgtttcccg cgtggtgaac caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg 5221 aagcggcgat ggcggagctg aattacattc ccaaccgcgt ggcacaacaa ctggcgggca 5281 aacagtcgtt gctgattggc gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa 5341 ttgtcgcggc gattaaatct cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg 5401 tagaacgaag cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg 5461 tcagtgggct gatcattaac tatccgctgg atgaccagga tgccattgct gtggaagctg 5521 cctgcactaa tgttccggcg ttatttcttg atgtctctga ccagacaccc atcaacagta 5581 ttattttctc ccatgaagac ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc 5641 accagcaaat cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg 5701 ctggctggca taaatatctc actcgcaatc aaattcagcc gatagcggaa cgggaaggcg 5761 actggagtgc catgtccggt tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc 5821 ccactgcgat gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc gccattaccg 5881 agtccgggct gcgcgttggt gcggatatct cggtagtggg atacgacgat accgaagaca 5941 gctcatgtta tatcccgccg ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa 6001 ccagcgtgga ccgcttgctg caactctctc agggccaggc ggtgaagggc aatcagctgt 6061 tgcccgtctc actggtgaaa agaaaaacca ccctggcgcc caatacgcaa accgcctctc 6121 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg 6181 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta 6241 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca 6301 ggaaacagct atgaccatga ttacggattc actggccgtc gttttacaac gtcgtgactg 6361 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg 6421 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg 6481 cgaatggcgc tttgcctggt ttccggcacc agaagcggtg ccggaaagct ggctggagtg 6541 cgatcttcct gaggccgata ctgtcgtcgt cccctcaaac tggcagatgc acggttacga 6601 tgcgcccatc tacaccaacg taacctatcc cattacggtc aatccgccgt ttgttcccac 6661 ggagaatccg acgggttgtt actcgctcac atttaatgtt gatgaaagct ggctacagga 6721 aggccagacg cgaattattt ttgatggcgt tggaatt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.