PiggyBac-TRE3GS-CEBPB-PGK-TetO

PiggyBac-TRE3GS-CEBPB-PGK-TetOn-EF1a-Puro plasmid

PVT96874

PiggyBac-TRE3GS-CEBPB-PGK-TetOn-EF1a-Puro plasmid Information

Plasmid name: PiggyBac-TRE3GS-CEBPB(human)-PGK-TetOn-EF1a-Puro
Alias: NM_005194.4;C/EBP-beta; IL6DBP; NF-IL6; TCF5
Gene lenght: 1038bp
Host: Mammalian cells
Use(s): Protein Expression,Tetracycline induction,Transposition
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: TRE3GS
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: TRE3GS-F1
Reverse primer:
Copy number: High
Induction:

PiggyBac-TRE3GS-CEBPB-PGK-TetOn-EF1a-Puro plasmid Description

PiggyBac-TRE3GS-CEBPB-PGK-TetOn-EF1a-Puro is a plasmid suitable for protein expression, transposition, and tetracycline induction in mammalian cells.

PiggyBac-TRE3GS-CEBPB-PGK-TetOn-EF1a-Puro plasmid Sequence

1 ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA 61 CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA 121 AGTGCCACCT AAATTGTAAG CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA 181 ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA 241 TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC 301 GTGGACTCCA ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA 361 CCATCACCCT AATCAAGTTT TTTGGGGTCG AGGTGCCGTA AAGCACTAAA TCGGAACCCT 421 AAAGGGAGCC CCCGATTTAG AGCTTGACGG GGAAAGCCGG CGAACGTGGC GAGAAAGGAA 481 GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT CACGCTGCGC 541 GTAACCACCA CACCCGCCGC GCTTAATGCG CCGCTACAGG GCGCGTCCCA TTCGCCATTC 601 AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG 661 GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA 721 CGACGTTGTA AAACGACGGC CAGTGAGCGC GCCTCGTTCA TTCACGTTTT TGAACCCGTG 781 GAGGACGGGC AGACTCGCGG TGCAAATGTG TTTTACAGCG TGATGGAGCA GATGAAGATG 841 CTCGACACGC TGCAGAACAC GCAGCTAGAT TAACCCTAGA AAGATAATCA TATTGTGACG 901 TACGTTAAAG ATAATCATGT GTAAAATTGA CGCATGTGTT TTATCGGTCT GTATATCGAG 961 GTTTATTTAT TAATTTGAAT AGATATTAAG TTTTATTATA TTTACACTTA CATACTAATA 1021 ATAAATTCAA CAAACAATTT ATTTATGTTT ATTTATTTAT TAAAAAAAAC AAAAACTCAA 1081 AATTTCTTCT ATAAAGTAAC AAAACTTTTA TGAGGGACAG CCCCCCCCCA AAGCCCCCAG 1141 GGATGTAATT ACGTCCCTCC CCCGCTAGGG GGCAGCAGCG AGCCGCCCGG GGCTCCGCTC 1201 CGGTCCGGCG CTCCCCCCGC ATCCCCGAGC CGGCAGCGTG CGGGGACAGC CCGGGCACGG 1261 GGAAGGTGGC ACGGGATCGC TTTCCTCTGA ACGCTTCTCG CTGCTCTTTG AGCCTGCAGA 1321 CACCTGGGGG GATACGGGGA AAAGGCCTCC AcGGCCACTA GTATTATGCC CAGTACATGA 1381 CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCaatt 1441 atcacctcga gtttactccc tatcagtgat agagaacgta tgaagagttt actccctatc 1501 agtgatagag aacgtatgca gactttactc cctatcagtg atagagaacg tataaggagt 1561 ttactcccta tcagtgatag agaacgtatg accagtttac tccctatcag tgatagagaa 1621 cgtatctaca gtttactccc tatcagtgat agagaacgta tatccagttt actccctatc 1681 agtgatagag aacgtataag ctttgcttat gtaaaccagg gcgcctataa aagagtgctg 1741 attttttgag taaacttcaa ttccacaaca cttttgtctt ataccaactt tccgtaccac 1801 ttcctaccct cgtaaagaat tcccgtatac accggtgcca ccatgcaacg cctggtggcc 1861 tgggacccag catgtctccc cctgccgccg ccgccgcctg cctttaaatc catggaagtg 1921 gccaacttct actacgaggc ggactgcttg gctgctgcgt acggcggcaa ggcggccccc 1981 gcggcgcccc ccgcggccag acccgggccg cgcccccccg ccggcgagct gggcagcatc 2041 ggcgaccacg agcgcgccat cgacttcagc ccgtacctgg agccgctggg cgcgccgcag 2101 gccccggcgc ccgccacggc cacggacacc ttcgaggcgg ctccgcccgc gcccgccccc 2161 gcgcccgcct cctccgggca gcaccacgac ttcctctccg acctcttctc cgacgactac 2221 gggggcaaga actgcaagaa gccggccgag tacggctacg tgagcctggg gcgcctgggg 2281 gccgccaagg gcgcgctgca ccccggctgc ttcgcgcccc tgcacccacc gcccccgccg 2341 ccgccgccgc ccgccgagct caaggcggag ccgggcttcg agcccgcgga ctgcaagcgg 2401 aaggaggagg ccggggcgcc gggcggcggc gcaggcatgg cggcgggctt cccgtacgcg 2461 ctgcgcgctt acctcggcta ccaggcggtg ccgagcggca gcagcgggag cctctccacg 2521 tcctcctcgt ccagcccgcc cggcacgccg agccccgctg acgccaaggc gcccccgacc 2581 gcctgctacg cgggggccgc gccggcgccc tcgcaggtca agagcaaggc caagaagacc 2641 gtggacaagc acagcgacga gtacaagatc cggcgcgagc gcaacaacat cgccgtgcgc 2701 aagagccgcg acaaggccaa gatgcgcaac ctggagacgc agcacaaggt cctggagctc 2761 acggccgaga acgagcggct gcagaagaag gtggagcagc tgtcgcgcga gctcagcacc 2821 ctgcggaact tgttcaagca gctgcccgag cccctgctcg cctcctccgg ccactgctag 2881 ggatccaCTC gaggtgataa ttccacgggg ttggggttgc gccttttcca aggcagccct 2941 gggtttgcgc agggacgcgg ctgctctggg cgtggttccg ggaaacgcag cggcgccgac 3001 cctgggtctc gcacattctt cacgtccgtt cgcagcgtca cccggatctt cgccgctacc 3061 cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa gtcgggaagg ttccttgcgg 3121 ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc tcactagtac cctcgcagac 3181 ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat gggctgtggc caatagcggc 3241 tgctcagcag ggcgcgccga gagcagcggc cgggaagggg cggtgcggga ggcggggtgt 3301 ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt ccgcattctg caagcctccg 3361 gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca ccgacctctc tccccagggg 3421 gatcatcgaa ttaccatgtc tagactggac aagagcaaag tcataaactc tgctctggaa 3481 ttactcaatg gagtcggtat cgaaggcctg acgacaagga aactcgctca aaagctggga 3541 gttgagcagc ctaccctgta ctggcacgtg aagaacaagc gggccctgct cgatgccctg 3601 ccaatcgaga tgctggacag gcatcatacc cactcctgcc ccctggaagg cgagtcatgg 3661 caagactttc tgcggaacaa cgccaagtca taccgctgtg ctctcctctc acatcgcgac 3721 ggggctaaag tgcatctcgg cacccgccca acagagaaac agtacgaaac cctggaaaat 3781 cagctcgcgt tcctgtgtca gcaaggcttc tccctggaga acgcactgta cgctctgtcc 3841 gccgtgggcc actttacact gggctgcgta ttggaggaac aggagcatca agtagcaaaa 3901 gaggaaagag agacacctac caccgattct atgcccccac ttctgaaaca agcaattgag 3961 ctgttcgacc ggcagggagc cgaacctgcc ttccttttcg gcctggaact aatcatatgt 4021 ggcctggaga aacagctaaa gtgcgaaagc ggcgggccga ccgacgccct tgacgatttt 4081 gacttagaca tgctcccagc cgatgccctt gacgactttg accttgatat gctgcctgct 4141 gacgctcttg acgattttga ccttgacatg ctccccgggt aaGCGGCCGC GAAGGATCTG 4201 CGATCGCTCC GGTGCCCGTC AGTGGGCAGA GCGCACATCG CCCACAGTCC CCGAGAAGTT 4261 GGGGGGAGGG GTCGGCAATT GAACGGGTGC CTAGAGAAGG TGGCGCGGGG TAAACTGGGA 4321 AAGTGATGTC GTGTACTGGC TCCGCCTTTT TCCCGAGGGT GGGGGAGAAC CGTATATAAG 4381 TGCAGTAGTC GCCGTGAACG TTCTTTTTCG CAACGGGTTT GCCGCCAGAA CACAGCTGAA 4441 GCTTCGAGGG GCTCGCATCT CTCCTTCACG CGCCCGCCGC CCTACCTGAG GCCGCCATCC 4501 ACGCCGGTTG AGTCGCGTTC TGCCGCCTCC CGCCTGTGGT GCCTCCTGAA CTGCGTCCGC 4561 CGTCTAGGTA AGTTTAAAGC TCAGGTCGAG ACCGGGCCTT TGTCCGGCGC TCCCTTGGAG 4621 CCTACCTAGA CTCAGCCGGC TCTCCACGCT TTGCCTGACC CTGCTTGCTC AACTCTACGT 4681 CTTTGTTTCG TTTTCTGTTC TGCGCCGTTA CAGATCCAAG CTGTGACCGG CGCCTACGCT 4741 AGACGCCACC ATGACCGAGT ACAAGCCCAC GGTGCGCCTC GCCACCCGCG ACGACGTCCC 4801 CAGGGCCGTA CGCACCCTCG CCGCCGCGTT CGCCGACTAC CCCGCCACGC GCCACACCGT 4861 CGATCCGGAC CGCCACATCG AGCGGGTCAC CGAGCTGCAA GAACTCTTCC TCACGCGCGT 4921 CGGGCTCGAC ATCGGCAAGG TGTGGGTCGC GGACGACGGC GCCGCGGTGG CGGTCTGGAC 4981 CACGCCGGAG AGCGTCGAAG CGGGGGCGGT GTTCGCCGAG ATCGGCCCGC GCATGGCCGA 5041 GTTGAGCGGT TCCCGGCTGG CCGCGCAGCA ACAGATGGAA GGCCTCCTGG CGCCGCACCG 5101 GCCCAAGGAG CCCGCGTGGT TCCTGGCCAC CGTCGGCGTC TCGCCCGACC ACCAGGGCAA 5161 GGGTCTGGGC AGCGCCGTCG TGCTCCCCGG AGTGGAGGCG GCCGAGCGCG CCGGGGTGCC 5221 CGCCTTCCTG GAGACCTCCG CGCCCCGCAA CCTCCCCTTC TACGAGCGGC TCGGCTTCAC 5281 CGTCACCGCC GACGTCGAGG TGCCCGAAGG ACCGCGCACC TGGTGCATGA CCCGCAAGCC 5341 CGGTGCCTGA AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG GTATTCTTAA 5401 CTATGTTGCT CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT ATCAGTTAAC 5461 TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT 5521 AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT 5581 CATGTCTGGA ATTGACTCAA ATGATGTCAA TTAGTCTATC AGAAGCTATC TGGTCTCCCT 5641 TCCGGGGGAC AAGACATCCC TGTTTAATAT TTAAACAGCA GTGTTCCCAA ACTGGGTTCT 5701 TATATCCCTT GCTCTGGTCA ACCAGGTTGC AGGGTTTCCT GTCCTCACAG GAACGAAGTC 5761 CCTAAAGAAA CAGTGGCAGC CAGGTTTAGC CCCGGAATTG ACTGGATTCC TTTTTTAGGG 5821 CCCATTGGTA TGGCTTTTTC CCCGTATCCC CCCAGGTGTC TGCAGGCTCA AAGAGCAGCG 5881 AGAAGCGTTC AGAGGAAAGC GATCCCGTGC CACCTTCCCC GTGCCCGGGC TGTCCCCGCA 5941 CGCTGCCGGC TCGGGGATGC GGGGGGAGCG CCGGACCGGA GCGGAGCCCC GGGCGGCTCG 6001 CTGCTGCCCC CTAGCGGGGG AGGGACGTAA TTACATCCCT GGGGGCTTTG GGGGGGGGCT 6061 GTCCCTGATA TCTATAACAA GAAAATATAT ATATAATAAG TTATCACGTA AGTAGAACAT 6121 GAAATAACAA TATAATTATC GTATGAGTTA AATCTTAAAA GTCACGTAAA AGATAATCAT 6181 GCGTCATTTT GACTCACGCG GTCGTTATAG TTCAAAATCA GTGACACTTA CCGCATTGAC 6241 AAGCACGCCT CACGGGAGCT CCAAGCGGCG ACTGAGATGT CCTAAATGCA CAGCGACGGA 6301 TTCGCGCTAT TTAGAAAGAG AGAGCAATAT TTCAAGAATG CATGCGTCAA TTTTACGCAG 6361 ACTATCTTTC TAGGGTTAAT CTAGCTGCAT CAGGATCATA TCGTCGGGTC TTTTTTCCGG 6421 CTCAGTCATC GCCCAAGCTG GCGCTATCTG GGCATCGGGG AGGAAGAAGC CCGTGCCTTT 6481 TCCCGCGAGG TTGAAGCGGC ATGGAAAGAG TTTGCCGAGG ATGACTGCTG CTGCATTGAC 6541 GTTGAGCGAA AACGCACGTT TACCATGATG ATTCGGGAAG GTGTGGCCAT GCACGCCTTT 6601 AACGGTGAAC TGTTCGTTCA GGCCACCTGG GATACCAGTT CGTCGCGGCT TTTCCGGACA 6661 CAGTTCCGGA TGGTCAGCCC GAAGCGCATC AGCAACCCGA ACAATACCGG CGACAGCCGG 6721 AACTGCCGTG CCGGTGTGCA GATTAATGAC AGCGGTGCGG CGCTGGGATA TTACGTCAGC 6781 GAGGACGGGT ATCCTGGCTG GATGCCGCAG AAATGGACAT GGATACCCCG TGAGTTACCC 6841 GGCGGGCGCG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC 6901 TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT 6961 GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC 7021 TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG 7081 GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG 7141 CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG 7201 GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC 7261 TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC 7321 AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC 7381 TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT 7441 CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG 7501 TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT 7561 CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG 7621 CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT 7681 GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC 7741 CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA 7801 GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG 7861 ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA 7921 TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA 7981 GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA 8041 TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC 8101 CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA 8161 TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA 8221 GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT 8281 GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG 8341 CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC 8401 AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG 8461 GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG 8521 CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT 8581 ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT 8641 CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC 8701 GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC 8761 CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG 8821 CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA 8881 TACTCAT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.