PiggyBac-TRE3GS-PPARG-PGK-TetO

PiggyBac-TRE3GS-PPARG-PGK-TetOn-EF1a-Puro plasmid

PVT96872

PiggyBac-TRE3GS-PPARG-PGK-TetOn-EF1a-Puro plasmid Information

Plasmid name: PiggyBac-TRE3GS-PPARG(human)-PGK-TetOn-EF1a-Puro
Alias: NM_015869.5;CIMT1; FPLD3; GLM1; NR1C3; PPARG1; PPARG2;PPARG5; PPARgamma
Gene lenght: 1508bp
Host: Mammalian cells
Use(s): Protein Expression,Tetracycline induction,Transposition
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: TRE3GS
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: TRE3GS-F1
Reverse primer:
Copy number: High
Induction:

PiggyBac-TRE3GS-PPARG-PGK-TetOn-EF1a-Puro plasmid Description

PiggyBac-TRE3GS-PPARG-PGK-TetOn-EF1a-Puro is a plasmid suitable for protein expression, transposition, and tetracycline induction in mammalian cells.

PiggyBac-TRE3GS-PPARG-PGK-TetOn-EF1a-Puro plasmid Sequence

1 ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA 61 CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA 121 AGTGCCACCT AAATTGTAAG CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA 181 ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA 241 TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC 301 GTGGACTCCA ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA 361 CCATCACCCT AATCAAGTTT TTTGGGGTCG AGGTGCCGTA AAGCACTAAA TCGGAACCCT 421 AAAGGGAGCC CCCGATTTAG AGCTTGACGG GGAAAGCCGG CGAACGTGGC GAGAAAGGAA 481 GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT CACGCTGCGC 541 GTAACCACCA CACCCGCCGC GCTTAATGCG CCGCTACAGG GCGCGTCCCA TTCGCCATTC 601 AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG 661 GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA 721 CGACGTTGTA AAACGACGGC CAGTGAGCGC GCCTCGTTCA TTCACGTTTT TGAACCCGTG 781 GAGGACGGGC AGACTCGCGG TGCAAATGTG TTTTACAGCG TGATGGAGCA GATGAAGATG 841 CTCGACACGC TGCAGAACAC GCAGCTAGAT TAACCCTAGA AAGATAATCA TATTGTGACG 901 TACGTTAAAG ATAATCATGT GTAAAATTGA CGCATGTGTT TTATCGGTCT GTATATCGAG 961 GTTTATTTAT TAATTTGAAT AGATATTAAG TTTTATTATA TTTACACTTA CATACTAATA 1021 ATAAATTCAA CAAACAATTT ATTTATGTTT ATTTATTTAT TAAAAAAAAC AAAAACTCAA 1081 AATTTCTTCT ATAAAGTAAC AAAACTTTTA TGAGGGACAG CCCCCCCCCA AAGCCCCCAG 1141 GGATGTAATT ACGTCCCTCC CCCGCTAGGG GGCAGCAGCG AGCCGCCCGG GGCTCCGCTC 1201 CGGTCCGGCG CTCCCCCCGC ATCCCCGAGC CGGCAGCGTG CGGGGACAGC CCGGGCACGG 1261 GGAAGGTGGC ACGGGATCGC TTTCCTCTGA ACGCTTCTCG CTGCTCTTTG AGCCTGCAGA 1321 CACCTGGGGG GATACGGGGA AAAGGCCTCC AcGGCCACTA GTATTATGCC CAGTACATGA 1381 CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCaatt 1441 atcacctcga gtttactccc tatcagtgat agagaacgta tgaagagttt actccctatc 1501 agtgatagag aacgtatgca gactttactc cctatcagtg atagagaacg tataaggagt 1561 ttactcccta tcagtgatag agaacgtatg accagtttac tccctatcag tgatagagaa 1621 cgtatctaca gtttactccc tatcagtgat agagaacgta tatccagttt actccctatc 1681 agtgatagag aacgtataag ctttgcttat gtaaaccagg gcgcctataa aagagtgctg 1741 attttttgag taaacttcaa ttccacaaca cttttgtctt ataccaactt tccgtaccac 1801 ttcctaccct cgtaaagaat tcccgtatac accggtgcca ccatgggtga aactctggga 1861 gattctccta ttgacccaga aagcgattcc ttcactgata cactgtctgc aaacatatca 1921 caagaaatga ccatggttga cacagagatg ccattctggc ccaccaactt tgggatcagc 1981 tccgtggatc tctccgtaat ggaagaccac tcccactcct ttgatatcaa gcccttcact 2041 actgttgact tctccagcat ttctactcca cattacgaag acattccatt cacaagaaca 2101 gatccagtgg ttgcagatta caagtatgac ctgaaacttc aagagtacca aagtgcaatc 2161 aaagtggagc ctgcatctcc accttattat tctgagaaga ctcagctcta caataagcct 2221 catgaagagc cttccaactc cctcatggca attgaatgtc gtgtctgtgg agataaagct 2281 tctggatttc actatggagt tcatgcttgt gaaggatgca agggtttctt ccggagaaca 2341 atcagattga agcttatcta tgacagatgt gatcttaact gtcggatcca caaaaaaagt 2401 agaaataaat gtcagtactg tcggtttcag aaatgccttg cagtggggat gtctcataat 2461 gccatcaggt ttgggcggat gccacaggcc gagaaggaga agctgttggc ggagatctcc 2521 agtgatatcg accagctgaa tccagagtcc gctgacctcc gggccctggc aaaacatttg 2581 tatgactcat acataaagtc cttcccgctg accaaagcaa aggcgagggc gatcttgaca 2641 ggaaagacaa cagacaaatc accattcgtt atctatgaca tgaattcctt aatgatggga 2701 gaagataaaa tcaagttcaa acacatcacc cccctgcagg agcagagcaa agaggtggcc 2761 atccgcatct ttcagggctg ccagtttcgc tccgtggagg ctgtgcagga gatcacagag 2821 tatgccaaaa gcattcctgg ttttgtaaat cttgacttga acgaccaagt aactctcctc 2881 aaatatggag tccacgagat catttacaca atgctggcct ccttgatgaa taaagatggg 2941 gttctcatat ccgagggcca aggcttcatg acaagggagt ttctaaagag cctgcgaaag 3001 ccttttggtg actttatgga gcccaagttt gagtttgctg tgaagttcaa tgcactggaa 3061 ttagatgaca gcgacttggc aatatttatt gctgtcatta ttctcagtgg agaccgccca 3121 ggtttgctga atgtgaagcc cattgaagac attcaagaca acctgctaca agccctggag 3181 ctccagctga agctgaacca ccctgagtcc tcacagctgt ttgccaagct gctccagaaa 3241 atgacagacc tcagacagat tgtcacggaa cacgtgcagc tactgcaggt gatcaagaag 3301 acggagacag acatgagtct tcacccgctc ctgcaggaga tctacaagga cttgtactag 3361 GGTACCggat ccaCTCgagg tgataattcc acggggttgg ggttgcgcct tttccaaggc 3421 agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa acgcagcggc 3481 gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg gatcttcgcc 3541 gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc 3601 ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac tagtaccctc 3661 gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc tgtggccaat 3721 agcggctgct cagcagggcg cgccgagagc agcggccggg aaggggcggt gcgggaggcg 3781 gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag 3841 cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga cctctctccc 3901 cagggggatc atcgaattac catgtctaga ctggacaaga gcaaagtcat aaactctgct 3961 ctggaattac tcaatggagt cggtatcgaa ggcctgacga caaggaaact cgctcaaaag 4021 ctgggagttg agcagcctac cctgtactgg cacgtgaaga acaagcgggc cctgctcgat 4081 gccctgccaa tcgagatgct ggacaggcat catacccact cctgccccct ggaaggcgag 4141 tcatggcaag actttctgcg gaacaacgcc aagtcatacc gctgtgctct cctctcacat 4201 cgcgacgggg ctaaagtgca tctcggcacc cgcccaacag agaaacagta cgaaaccctg 4261 gaaaatcagc tcgcgttcct gtgtcagcaa ggcttctccc tggagaacgc actgtacgct 4321 ctgtccgccg tgggccactt tacactgggc tgcgtattgg aggaacagga gcatcaagta 4381 gcaaaagagg aaagagagac acctaccacc gattctatgc ccccacttct gaaacaagca 4441 attgagctgt tcgaccggca gggagccgaa cctgccttcc ttttcggcct ggaactaatc 4501 atatgtggcc tggagaaaca gctaaagtgc gaaagcggcg ggccgaccga cgcccttgac 4561 gattttgact tagacatgct cccagccgat gcccttgacg actttgacct tgatatgctg 4621 cctgctgacg ctcttgacga ttttgacctt gacatgctcc ccgggtaaGC GGCCGCGAAG 4681 GATCTGCGAT CGCTCCGGTG CCCGTCAGTG GGCAGAGCGC ACATCGCCCA CAGTCCCCGA 4741 GAAGTTGGGG GGAGGGGTCG GCAATTGAAC GGGTGCCTAG AGAAGGTGGC GCGGGGTAAA 4801 CTGGGAAAGT GATGTCGTGT ACTGGCTCCG CCTTTTTCCC GAGGGTGGGG GAGAACCGTA 4861 TATAAGTGCA GTAGTCGCCG TGAACGTTCT TTTTCGCAAC GGGTTTGCCG CCAGAACACA 4921 GCTGAAGCTT CGAGGGGCTC GCATCTCTCC TTCACGCGCC CGCCGCCCTA CCTGAGGCCG 4981 CCATCCACGC CGGTTGAGTC GCGTTCTGCC GCCTCCCGCC TGTGGTGCCT CCTGAACTGC 5041 GTCCGCCGTC TAGGTAAGTT TAAAGCTCAG GTCGAGACCG GGCCTTTGTC CGGCGCTCCC 5101 TTGGAGCCTA CCTAGACTCA GCCGGCTCTC CACGCTTTGC CTGACCCTGC TTGCTCAACT 5161 CTACGTCTTT GTTTCGTTTT CTGTTCTGCG CCGTTACAGA TCCAAGCTGT GACCGGCGCC 5221 TACGCTAGAC GCCACCATGA CCGAGTACAA GCCCACGGTG CGCCTCGCCA CCCGCGACGA 5281 CGTCCCCAGG GCCGTACGCA CCCTCGCCGC CGCGTTCGCC GACTACCCCG CCACGCGCCA 5341 CACCGTCGAT CCGGACCGCC ACATCGAGCG GGTCACCGAG CTGCAAGAAC TCTTCCTCAC 5401 GCGCGTCGGG CTCGACATCG GCAAGGTGTG GGTCGCGGAC GACGGCGCCG CGGTGGCGGT 5461 CTGGACCACG CCGGAGAGCG TCGAAGCGGG GGCGGTGTTC GCCGAGATCG GCCCGCGCAT 5521 GGCCGAGTTG AGCGGTTCCC GGCTGGCCGC GCAGCAACAG ATGGAAGGCC TCCTGGCGCC 5581 GCACCGGCCC AAGGAGCCCG CGTGGTTCCT GGCCACCGTC GGCGTCTCGC CCGACCACCA 5641 GGGCAAGGGT CTGGGCAGCG CCGTCGTGCT CCCCGGAGTG GAGGCGGCCG AGCGCGCCGG 5701 GGTGCCCGCC TTCCTGGAGA CCTCCGCGCC CCGCAACCTC CCCTTCTACG AGCGGCTCGG 5761 CTTCACCGTC ACCGCCGACG TCGAGGTGCC CGAAGGACCG CGCACCTGGT GCATGACCCG 5821 CAAGCCCGGT GCCTGAAATC AACCTCTGGA TTACAAAATT TGTGAAAGAT TGACTGGTAT 5881 TCTTAACTAT GTTGCTCCTT TTACGCTATG TGGATACGCT GCTTTAATGC CTTTGTATCA 5941 GTTAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC 6001 ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA 6061 TCTTATCATG TCTGGAATTG ACTCAAATGA TGTCAATTAG TCTATCAGAA GCTATCTGGT 6121 CTCCCTTCCG GGGGACAAGA CATCCCTGTT TAATATTTAA ACAGCAGTGT TCCCAAACTG 6181 GGTTCTTATA TCCCTTGCTC TGGTCAACCA GGTTGCAGGG TTTCCTGTCC TCACAGGAAC 6241 GAAGTCCCTA AAGAAACAGT GGCAGCCAGG TTTAGCCCCG GAATTGACTG GATTCCTTTT 6301 TTAGGGCCCA TTGGTATGGC TTTTTCCCCG TATCCCCCCA GGTGTCTGCA GGCTCAAAGA 6361 GCAGCGAGAA GCGTTCAGAG GAAAGCGATC CCGTGCCACC TTCCCCGTGC CCGGGCTGTC 6421 CCCGCACGCT GCCGGCTCGG GGATGCGGGG GGAGCGCCGG ACCGGAGCGG AGCCCCGGGC 6481 GGCTCGCTGC TGCCCCCTAG CGGGGGAGGG ACGTAATTAC ATCCCTGGGG GCTTTGGGGG 6541 GGGGCTGTCC CTGATATCTA TAACAAGAAA ATATATATAT AATAAGTTAT CACGTAAGTA 6601 GAACATGAAA TAACAATATA ATTATCGTAT GAGTTAAATC TTAAAAGTCA CGTAAAAGAT 6661 AATCATGCGT CATTTTGACT CACGCGGTCG TTATAGTTCA AAATCAGTGA CACTTACCGC 6721 ATTGACAAGC ACGCCTCACG GGAGCTCCAA GCGGCGACTG AGATGTCCTA AATGCACAGC 6781 GACGGATTCG CGCTATTTAG AAAGAGAGAG CAATATTTCA AGAATGCATG CGTCAATTTT 6841 ACGCAGACTA TCTTTCTAGG GTTAATCTAG CTGCATCAGG ATCATATCGT CGGGTCTTTT 6901 TTCCGGCTCA GTCATCGCCC AAGCTGGCGC TATCTGGGCA TCGGGGAGGA AGAAGCCCGT 6961 GCCTTTTCCC GCGAGGTTGA AGCGGCATGG AAAGAGTTTG CCGAGGATGA CTGCTGCTGC 7021 ATTGACGTTG AGCGAAAACG CACGTTTACC ATGATGATTC GGGAAGGTGT GGCCATGCAC 7081 GCCTTTAACG GTGAACTGTT CGTTCAGGCC ACCTGGGATA CCAGTTCGTC GCGGCTTTTC 7141 CGGACACAGT TCCGGATGGT CAGCCCGAAG CGCATCAGCA ACCCGAACAA TACCGGCGAC 7201 AGCCGGAACT GCCGTGCCGG TGTGCAGATT AATGACAGCG GTGCGGCGCT GGGATATTAC 7261 GTCAGCGAGG ACGGGTATCC TGGCTGGATG CCGCAGAAAT GGACATGGAT ACCCCGTGAG 7321 TTACCCGGCG GGCGCGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT 7381 ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG 7441 CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG 7501 GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC 7561 GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC 7621 GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA 7681 ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG 7741 CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT 7801 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA 7861 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC 7921 TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT 7981 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG 8041 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG 8101 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT 8161 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC 8221 TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG 8281 CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC 8341 AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT 8401 AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA 8461 AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT 8521 GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT 8581 GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG 8641 CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG 8701 CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA 8761 ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG 8821 CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG 8881 GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT 8941 CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA 9001 TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG 9061 GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC 9121 CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG 9181 GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA 9241 TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG 9301 GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT 9361 GTTGAATACT CAT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.