pLV3-CMV-Cdk5rap2-3×FLAG-CopGF

pLV3-CMV-Cdk5rap2-3×FLAG-CopGFP-Puro plasmid

PVT96873

pLV3-CMV-Cdk5rap2-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Cdk5rap2(mouse)-3×FLAG-CopGFP-Puro
Alias: NM_145990.4;2900018K03Rik; an; mKIAA1633
Gene lenght: 5469bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Cdk5rap2-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-Cdk5rap2-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-Cdk5rap2-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccatgg actcggggat ggaagaggaa 2341 ggcgccctgc ctgggaccct cagcggttgc agtggccttc accctgttct gccaagtgac 2401 ctggatgtca tcagtgacac ttctggcttg ggaaatggag tgctcccaag catgtcagaa 2461 gaaaaggttt ctcccaccag agcccggaac atgaaggact ttgaaaacca aatcactgag 2521 ctgaagaagg agaacttcaa tctaaagctc cggatctact tcctggagga gagaatccag 2581 caggagtttg ctggccccac cgagcacatc tacaagacga acattgaact caaggtggaa 2641 gtggaaagtc tgaagcgcga gctccaggag aaagatcagt tgctcgtcaa agcctccaaa 2701 gcggtggaga gcctagcaga acggggtggt tctgaagtcc agcgggtgaa agaagatgca 2761 aggaagaagg tgcagcaggt ggaagacctc ctaacgaaaa ggatacatct tctagaagaa 2821 gatgtgaaag ccgcccaggc agaactggag aaggcctttg cggggacaga aacagagaag 2881 gctcttcggc tcagcttaga aagcaagctc tcggcgatga agaagatgca ggagggggac 2941 ctggagatga ctctggctct ggaagagaag gacagactga ttgaggagtt gaagctgtct 3001 ttgaagagca aagaagcttt aattcagtgc cttaaagagg aaaaatctca gatggcatct 3061 cctgacgaga acgtgtcatc tggagaactc cgaggacttt ctgccactct gagggaagaa 3121 aaagagagag acgctgagga atggcagaag gagagaaacc actttgaaga gaggatccag 3181 gcacttcagg aggacctgag agagaaggaa agagaaattg ctacagagaa gaaaaataac 3241 ttaaagagtt ataaagccat tcagggtcta accatggcat taaaatcaaa ggaaagagag 3301 gttgaagaac ttgattccaa aatcaaagag gtcactacgg actccaccaa aggcagagag 3361 gatcccttaa aaacccagat ccccagattc cagctaagag aggggtctga ggactgtgaa 3421 gctgcgctgg tagagaagga agccctattg gccaagctac actcagaaaa tgtcacaaag 3481 agcactgaga accataggtt gctcaggaat gttaaaaagg tcacccagga gttgaatgac 3541 ttgaagaagg agaagttgag actagagcag gccctggagg aagcccacca agaggggaac 3601 agaggagccc gaaccattca cgaccttaga aatgaagttg agaaactacg caaggaagtg 3661 agtgagagag agaaggctgt ggaaaaacat tacaagagtc ttccaggtga aagcaagaca 3721 aagtttcata ctcaagaaca agtggtcaga agtctgacag gcagtggcag tcaggaggac 3781 ctgctgcttc agaaatccaa tgaaaaagat ttggaagcaa tacagcagaa ctgctattta 3841 atgactgcag aggagctcaa gtttgggagc gatggcttaa taacagaaaa gtgttctcag 3901 cagtcaccag acagcaaact catcttctcc aaggaaaagc aacaatcaga gtatgaaggg 3961 ctgactgggg acttaaaagc agaacagaac atctatgccc atctggcaaa gactcaggac 4021 acagacagtg tcagcaacct gcaggctgag ctgaaagagg tccttgccct tcggaagcag 4081 ctggagcagg atgttcttgc ctaccggaac ctgcagaagg ccctgcagga gcagctcagt 4141 gagattcgga gtcgagaaga agagccgttt tcattttata gcgatcaaac atcgtatctg 4201 agtatttgcc ttgaagagca caatcagttc caattggagc atttttctca agaagaactc 4261 aagaaaaagg tcagtgacct catacagctt gtgaaggacc tgcacacaga caaccagcac 4321 ctgaagaaaa ccatttttga tctctcttct gtgggtttcc agggaagtga cagacttgag 4381 ttaacaaaac aagaagagct tgtggctagc aaggaggatg aggacacact caaatttgaa 4441 gcggacgttg agactccttt ccagagtgac cagcatttgg agcaaagtcg tgagatcatg 4501 gaggactatg ctgagggtgg ctgcaaaagt ggatatggaa ggcacatgga ttccaatatt 4561 ttaggtcatg atggggccca aactcctggt gcctctgaag agcacaccct agaagatgag 4621 ctcctgggcc tgcttgccac tctcttcagt aagaaggcca caccgttgct ggaatccagg 4681 ccagatcttc tgaaagcact cggtgcccta cttttggaac gcatatgctt ggcagagcag 4741 ggtcgtcctg gagatcacct tgatagtaaa actgagaagg cacttcagca agtagctgtt 4801 cagttgagag atgaacttgg ccattccttc cctgccaact cgttctccaa gtcatacaat 4861 gaagtcaagt caatgtgggg aaattggctg gtaaagacag gagacgaaga cacggtggag 4921 ctgaagagtg tttcagtgca gaccatggcc atagaggaca ccccgcatgg attcaaacct 4981 caaagcaaga gagatgcttg ggcagagaaa caagaagagg ccattttcag cactgagctt 5041 gagtcagagg cgttgggtga gatgccaggg caacaggcca ctcacctctc attccccagt 5101 gccatcaacc cggatgccga gaagacgggc ttgttgatcc aattaaagac acctgagtta 5161 ttggaaaatt tatataatct tcctgcttcg ccagaggtgg tggtggctca actccaaggc 5221 caggttctag agctgcagag ggagctgaaa gaattcaaaa cccgcaacaa gcaactccat 5281 gagaagctaa ttctggcaga ggcaatgatg gaggggctgc cggtgcccaa ctctgcgctg 5341 gtgaatgtgc ctgcagccca ggctgttgtg agaacagcct tccaggacaa cccaggcgag 5401 caagaaggac ctgaaaccac acagtctgct gggagagaca aggacatgga cagtgaccaa 5461 tatacaagct ttgagatcga ttctgaaatc tgtccacctg atgaccttgc cttattacca 5521 gcatgcaaag agaatcttga agacctccta ggcccatcct ccatagccac ttacctggat 5581 tccaagagtc agctctctgt taaagtcagc gtgaatggaa ctgaccagtc tgagaatatc 5641 aatatccctg atgacacaga agacttaaaa cagaaaatcc acgacttgca aaccgagctg 5701 gaaggctacc ggaacatcat tgtccagcta ctgaagcact ctcagtgcag tgaggctatt 5761 atcacagttt tgtgtgggac agaaggggcc caagatggct tgaacaagcc taagggccat 5821 attgatgagg aagagatgac attttcaagt ttgcaccaag tacgatatgt gaagcatatg 5881 aaaatcctcc gtccactaac cccggagatg attgatggca agatgctaga gagcctcaag 5941 cagcagctgg tggaccagga gcaggagcta cagaaggagc aggacttgaa cttggaactt 6001 ttcggtgaaa tccatgacct gcagaacaag ttccaagacc tctcgccatc aagatacgat 6061 tcattggttc agtcccaagc gagggagctc tcccttcagc gacagcaaat taaggatagc 6121 catggcatct gtgtcattta ccgtcaacac atgagcacca tgatcaaggc gtttgaggag 6181 cttctgcagg ccagtgatgt ggacagttgc gtggccgagg gcttccggga gcagctgaca 6241 cagtgtgctg gactgctaga gcaactggag aggctgtttc tccatggaaa atcagccaga 6301 gtggaaccac atccccagaa tgaactgctc aaggggttaa ggactgtgga aggcaactta 6361 ccctaccacc atctcctgcc tgaatctcct gagccttctg cctcccatgc tctgtcagat 6421 gatgaaatgt ctgagaagtc cttcctgtct agagacccga agccagacag cgatactgag 6481 aagtacccag caatggcgag ccattttcct caagacttac taatggaaca catacaggaa 6541 attcgaactt tgagaaagca tttggaagaa tctattaaaa caaatgagaa gctccggaaa 6601 cagctggaac ggcaggggtc tgagactgac caaggctcca gaaatgtttc tgcttgtggc 6661 ttagcactgc acagctctct gacgtcagag atacatttcc tgaggaagca gaacgaggcc 6721 ctcagtatga tgctcgagaa ggggtccaaa gataaacaga aagagagtga aaaattacgt 6781 gagtccctgg ctaggaaagc tgagagtctt gagcaacttc agctagagta caccagtgta 6841 agggaagaaa atgaaaggtt acagagagac atcattgaga aggagagaca caaccaggag 6901 ctgactgagg aggtctgcag cagccgccag gaactgagca gggtgcagga agaggcgaag 6961 tcaagacagc agctgctctc acagaaggac aagctgcttc agtccctcca aatggagctc 7021 aaggtgtatg agaaactggc cgaggaacat ccgaggctac agcaagatgg cagcaaatgt 7081 cctgaggcca gcgacaattc atttgatcta tttgaatcca cccaggcaat ggcacctaaa 7141 tcagcttctg agactcctct gctctcagga actgatgttg actcgctctc ctgtgacagt 7201 accagttctg ccaccagccc tactagcatg ccctgcctgg ttgctggcca ccacatgtgg 7261 gccagcaaga gtggccacca tatgctgggc ctgattgagg actatgatgc cctctataag 7321 cagatcagct ggggccagac actgcttgcc aagatggatg ttcaaaccca agaggctctg 7381 agccccacaa gtcacaagct gggaccaaag ggttcttcat ccgtgcctct gagcaagttt 7441 ctttccagta tgaacacagc caagctggtc ctggaaaaag catccaggtt gctgaagctc 7501 ttctggaggg tctcagtccc caccaacggc cagtgttccc ttcactgtga gcagattgga 7561 gaaatgaagg cagagaacac gaaactacac aaaaaattgt ttgaacaaga aaagaagctg 7621 cagaacacag caaaactatt gcagcagagc aagcaccagg agaaagtcat ctttgatcag 7681 ttggtcatca cccaccaagt ccttcggaag gccaggggaa acctggagct caggcctggg 7741 gccacccgtc caggagcatc cagtcccagc agaccgggct caggatccGA CTACAAAGAC 7801 CATGACGGTG ATTATAAAGA TCATGACATC GACTACAAGG ATGACGATGA CAAGtagcgg 7861 ccgcGaagga tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca 7921 gtccccgaga agttgggggg aggggtcggc aattgaacgg gtgcctagag aaggtggcgc 7981 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga 8041 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc 8101 agaacacagc tgaagcttcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc 8161 tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc 8221 tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg 8281 gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt 8341 gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc caagctgtga 8401 ccggcgccta cgctagacgc caccatggag agcgacgaga gcggcctgcc cgccatggag 8461 atcgagtgcc gcatcaccgg caccctgaac ggcgtggagt tcgagctggt gggcggcgga 8521 gagggcaccc ccaagcaggg ccgcatgacc aacaagatga agagcaccaa aggcgccctg 8581 accttcagcc cctacctgct gagccacgtg atgggctacg gcttctacca cttcggcacc 8641 taccccagcg gctacgagaa ccccttcctg cacgccatca acaacggcgg ctacaccaac 8701 acccgcatcg agaagtacga ggacggcggc gtgctgcacg tgagcttcag ctaccgctac 8761 gaggccggcc gcgtgatcgg cgacttcaag gtggtgggca ccggcttccc cgaggacagc 8821 gtgatcttca ccgacaagat catccgcagc aacgccaccg tggagcacct gcaccccatg 8881 ggcgataacg tgctggtggg cagcttcgcc cgcaccttca gcctgcgcga cggcggctac 8941 tacagcttcg tggtggacag ccacatgcac ttcaagagcg ccatccaccc cagcatcctg 9001 cagaacgggg gccccatgtt cgccttccgc cgcgtggagg agctgcacag caacaccgag 9061 ctgggcatcg tggagtacca gcacgccttc aagaccccca tcgccttcgc cagatcccgc 9121 gctcagtcgt ccaattctgc cgtggacggc accgccggac ccggctccac cggatctcgc 9181 gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctatgacc 9241 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc 9301 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac 9361 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc 9421 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc 9481 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg 9541 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg 9601 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc 9661 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc 9721 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc 9781 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaaatcaa 9841 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt 9901 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct 9961 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc 10021 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg 10081 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc 10141 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc 10201 actgacaatt ccgtggtgtt gtcggggaaA TCATCGTCCT TTCCTtggct gctcgcctgt 10261 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca 10321 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tctTcgcctt 10381 cgccctcaga cgagtcggat ctccctttgg Gccgcctccc cgcctggtac ctttaagacc 10441 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga 10501 agggctaatt cactcccaac gaaGataaga tctgcttttt gcttgtactg ggtctctctg 10561 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc 10621 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg 10681 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc 10741 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag 10801 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac 10861 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc 10921 ttatcatgtc tggctctagc taTCCCGCCC CTAACTCCGC CCAtcccgcc cctaactccg 10981 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc 11041 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta 11101 gacttttgca gagaccaaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta 11161 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc 11221 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg 11281 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg 11341 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 11401 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 11461 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 11521 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 11581 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 11641 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 11701 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 11761 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 11821 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 11881 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 11941 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct 12001 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 12061 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 12121 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 12181 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 12241 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 12301 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 12361 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 12421 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 12481 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 12541 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 12601 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 12661 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 12721 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 12781 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 12841 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 12901 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 12961 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 13021 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 13081 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 13141 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 13201 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 13261 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta 13321 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa 13381 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag 13441 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta 13501 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag 13561 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg 13621 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc 13681 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac 13741 ggccagtgcc aagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.