pLV3-CMV-IGF2BP3-3×FLAG-EF1a-C

pLV3-CMV-IGF2BP3-3×FLAG-EF1a-CopGFP-Puro plasmid

PVT94056

pLV3-CMV-IGF2BP3-3×FLAG-EF1a-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-IGF2BP3(human)-3×FLAG-EF1a-CopGFP-Puro
Alias: NM_006547.3;CT98; IMP-3; IMP3; KOC; KOC1; VICKZ3
Gene lenght: 1737bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-IGF2BP3-3×FLAG-EF1a-CopGFP-Puro plasmid Description

pLV3-CMV-IGF2BP3-3×FLAG-EF1a-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-IGF2BP3-3×FLAG-EF1a-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccatga acaaactgta tatcggaaac 2341 ctcagcgaga acgccgcccc ctcggaccta gaaagtatct tcaaggacgc caagatcccg 2401 gtgtcgggac ccttcctggt gaagactggc tacgcgttcg tggactgccc ggacgagagc 2461 tgggccctca aggccatcga ggcgctttca ggtaaaatag aactgcacgg gaaacccata 2521 gaagttgagc actcggtccc aaaaaggcaa aggattcgga aacttcagat acgaaatatc 2581 ccgcctcatt tacagtggga ggtgctggat agtttactag tccagtatgg agtggtggag 2641 agctgtgagc aagtgaacac tgactcggaa actgcagttg taaatgtaac ctattccagt 2701 aaggaccaag ctagacaagc actagacaaa ctgaatggat ttcagttaga gaatttcacc 2761 ttgaaagtag cctatatccc tgatgaaatg gccgcccagc aaaacccctt gcagcagccc 2821 cgaggtcgcc gggggcttgg gcagaggggc tcctcaaggc aggggtctcc aggatccgta 2881 tccaagcaga aaccatgtga tttgcctctg cgcctgctgg ttcccaccca atttgttgga 2941 gccatcatag gaaaagaagg tgccaccatt cggaacatca ccaaacagac ccagtctaaa 3001 atcgatgtcc accgtaaaga aaatgcgggg gctgctgaga agtcgattac tatcctctct 3061 actcctgaag gcacctctgc ggcttgtaag tctattctgg agattatgca taaggaagct 3121 caagatataa aattcacaga agagatcccc ttgaagattt tagctcataa taactttgtt 3181 ggacgtctta ttggtaaaga aggaagaaat cttaaaaaaa ttgagcaaga cacagacact 3241 aaaatcacga tatctccatt gcaggaattg acgctgtata atccagaacg cactattaca 3301 gttaaaggca atgttgagac atgtgccaaa gctgaggagg agatcatgaa gaaaatcagg 3361 gagtcttatg aaaatgatat tgcttctatg aatcttcaag cacatttaat tcctggatta 3421 aatctgaacg ccttgggtct gttcccaccc acttcaggga tgccacctcc cacctcaggg 3481 cccccttcag ccatgactcc tccctacccg cagtttgagc aatcagaaac ggagactgtt 3541 catctgttta tcccagctct atcagtcggt gccatcatcg gcaagcaggg ccagcacatc 3601 aagcagcttt ctcgctttgc tggagcttca attaagattg ctccagcgga agcaccagat 3661 gctaaagtga ggatggtgat tatcactgga ccaccagagg ctcagttcaa ggctcaggga 3721 agaatttatg gaaaaattaa agaagaaaac tttgttagtc ctaaagaaga ggtgaaactt 3781 gaagctcata tcagagtgcc atcctttgct gctggcagag ttattggaaa aggaggcaaa 3841 acggtgaatg aacttcagaa tttgtcaagt gcagaagttg ttgtccctcg tgaccagaca 3901 cctgatgaga atgaccaagt ggttgtcaaa ataactggtc acttctatgc ttgccaggtt 3961 gcccagagaa aaattcagga aattctgact caggtaaagc agcaccaaca acagaaggct 4021 ctgcaaagtg gaccacctca gtcaagacgg aagggatccG ACTACAAAGA CCATGACGGT 4081 GATTATAAAG ATCATGACAT CGACTACAAG GATGACGATG ACAAGtagcg gccgcGaagg 4141 atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag 4201 aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac 4261 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat 4321 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag 4381 ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc 4441 catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg 4501 tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct 4561 tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc 4621 tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct 4681 acgctagacg ccaccatgga gagcgacgag agcggcctgc ccgccatgga gatcgagtgc 4741 cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg agagggcacc 4801 cccaagcagg gccgcatgac caacaagatg aagagcacca aaggcgccct gaccttcagc 4861 ccctacctgc tgagccacgt gatgggctac ggcttctacc acttcggcac ctaccccagc 4921 ggctacgaga accccttcct gcacgccatc aacaacggcg gctacaccaa cacccgcatc 4981 gagaagtacg aggacggcgg cgtgctgcac gtgagcttca gctaccgcta cgaggccggc 5041 cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc ccgaggacag cgtgatcttc 5101 accgacaaga tcatccgcag caacgccacc gtggagcacc tgcaccccat gggcgataac 5161 gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta ctacagcttc 5221 gtggtggaca gccacatgca cttcaagagc gccatccacc ccagcatcct gcagaacggg 5281 ggccccatgt tcgccttccg ccgcgtggag gagctgcaca gcaacaccga gctgggcatc 5341 gtggagtacc agcacgcctt caagaccccc atcgccttcg ccagatcccg cgctcagtcg 5401 tccaattctg ccgtggacgg caccgccgga cccggctcca ccggatctcg cgagggcaga 5461 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctatgac cgagtacaag 5521 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc 5581 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg 5641 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg 5701 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg 5761 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg 5821 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg 5881 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc 5941 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc 6001 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc 6061 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaaatca acctctggat 6121 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt 6181 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc 6241 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg 6301 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc 6361 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa 6421 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat 6481 tccgtggtgt tgtcggggaa ATCATCGTCC TTTCCTtggc tgctcgcctg tgttgccacc 6541 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt 6601 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtctTcgcct tcgccctcag 6661 acgagtcgga tctccctttg gGccgcctcc ccgcctggta cctttaagac caatgactta 6721 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat 6781 tcactcccaa cgaaGataag atctgctttt tgcttgtact gggtctctct ggttagacca 6841 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag 6901 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag 6961 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct 7021 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt 7081 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc 7141 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt 7201 ctggctctag ctaTCCCGCC CCTAACTCCG CCCAtcccgc ccctaactcc gcccagttcc 7261 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc 7321 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc 7381 agagaccaaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac 7441 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 7501 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 7561 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 7621 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 7681 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 7741 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 7801 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 7861 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 7921 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 7981 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 8041 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 8101 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 8161 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 8221 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 8281 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 8341 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 8401 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 8461 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 8521 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 8581 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 8641 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 8701 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 8761 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 8821 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 8881 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 8941 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 9001 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 9061 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 9121 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 9181 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 9241 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 9301 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 9361 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 9421 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 9481 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 9541 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag 9601 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca 9661 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc 9721 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc 9781 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag 9841 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg 9901 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg 9961 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc 10021 caagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.