pLV3-CMV-GPAA1-3×FLAG-COpGFP-P

pLV3-CMV-GPAA1-3×FLAG-COpGFP-Puro plasmid

PVT96870

pLV3-CMV-GPAA1-3×FLAG-COpGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-GPAA1(human)-3×FLAG-COpGFP-Puro
Alias: NM_003801.4;GAA1; GPIBD15; hGAA1
Gene lenght: 1863bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-GPAA1-3×FLAG-COpGFP-Puro plasmid Description

pLV3-CMV-GPAA1-3×FLAG-COpGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-GPAA1-3×FLAG-COpGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg gcctcctgtc ggacccggtt 2341 cgccggcgcg cgctcgcccg cctagtgctg cgcctcaacg cgccgttgtg cgtgctgagc 2401 tacgtggcgg gcatcgcctg gttcttggcg ctggttttcc cgccgctgac ccagcgcact 2461 tacatgtcgg agaacgccat gggctccacc atggtggagg agcagtttgc gggcggagac 2521 cgtgcccggg cttttgcccg ggacttcgcc gcccaccgca agaagtcggg ggctctgcca 2581 gtggcctggc ttgaacggac gatgcggtca gtagggctgg aggtctacac gcagagtttc 2641 tcccggaaac tgcccttccc agatgagacc cacgagcgct atatggtgtc gggcaccaac 2701 gtgtacggca tcctgcgggc cccgcgtgct gccagcaccg agtcgcttgt gctcaccgtg 2761 ccctgtggct ctgactctac caacagccag gctgtggggc tgctgctggc actggctgcc 2821 cacttccggg ggcagattta ttgggccaaa gatatcgtct tcctggtaac agaacatgac 2881 cttctgggca ctgaggcttg gcttgaagcc taccacgatg tcaatgtcac tggcatgcag 2941 tcgtctcccc tgcagggccg agctggggcc attcaggcag ccgtggccct ggagctgagc 3001 agtgatgtgg tcaccagcct cgatgtggcc gtggaggggc ttaacgggca gctgcccaac 3061 cttgacctgc tcaatctctt ccagaccttc tgccagaaag ggggcctgtt gtgcacgctt 3121 cagggcaagc tgcagcccga ggactggaca tcattggatg gaccgctgca gggcctgcag 3181 acactgctgc tcatggttct gcggcaggcc tccggccgcc cccacggctc ccatggcctc 3241 ttcctgcgct accgtgtgga ggccctaacc ctgcgtggca tcaatagctt ccgccagtac 3301 aagtatgacc tggtggcagt gggcaaggct ttggagggca tgttccgcaa gctcaaccac 3361 ctcctggagc gcctgcacca gtccttcttc ctctacttgc tccccggcct ctcccgcttc 3421 gtctccatcg gcctctacat gcccgctgtc ggcttcttgc tcctggtcct tggtctcaag 3481 gctctggaac tgtggatgca gctgcatgag gctggaatgg gccttgagga gcccgggggt 3541 gcccctggcc ccagtgtacc ccttccccca tcacagggtg tggggctggc ctcgctcgtg 3601 gcacctctgc tgatctcaca ggccatggga ctggccctct atgtcctgcc agtgctgggc 3661 caacacgttg ccacccagca cttcccagtg gcagaggctg aggctgtggt gctgacactg 3721 ctggcgattt atgcagctgg cctggccctg ccccacaata cccaccgggt ggtaagcaca 3781 caggccccag acaggggctg gatggcactg aagctggtag ccctgatcta cctagcactg 3841 cagctgggct gcatcgccct caccaacttc tcactgggct tcctgctggc caccaccatg 3901 gtgcccactg ctgcgcttgc caagcctcat gggccccgga ccctctatgc tgccctgctg 3961 gtgctgacca gcccggcagc cacgctcctt ggcagcctgt tcctgtggcg ggagctgcag 4021 gaggcgccac tgtcactggc cgagggctgg cagctcttcc tggcagcgct agcccagggt 4081 gtgctggagc accacaccta cggcgccctg ctcttcccac tgctgtccct gggcctctac 4141 ccctgctggc tgcttttctg gaatgtgctc ttctggaagg gatccGACTA CAAAGACCAT 4201 GACGGTGATT ATAAAGATCA TGACATCGAC TACAAGGATG ACGATGACAA Gtagcggccg 4261 cGaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc 4321 cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg 4381 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa 4441 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga 4501 acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga 4561 ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga 4621 actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg 4681 ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct 4741 caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg 4801 gcgcctacgc tagacgccac catggagagc gacgagagcg gcctgcccgc catggagatc 4861 gagtgccgca tcaccggcac cctgaacggc gtggagttcg agctggtggg cggcggagag 4921 ggcaccccca agcagggccg catgaccaac aagatgaaga gcaccaaagg cgccctgacc 4981 ttcagcccct acctgctgag ccacgtgatg ggctacggct tctaccactt cggcacctac 5041 cccagcggct acgagaaccc cttcctgcac gccatcaaca acggcggcta caccaacacc 5101 cgcatcgaga agtacgagga cggcggcgtg ctgcacgtga gcttcagcta ccgctacgag 5161 gccggccgcg tgatcggcga cttcaaggtg gtgggcaccg gcttccccga ggacagcgtg 5221 atcttcaccg acaagatcat ccgcagcaac gccaccgtgg agcacctgca ccccatgggc 5281 gataacgtgc tggtgggcag cttcgcccgc accttcagcc tgcgcgacgg cggctactac 5341 agcttcgtgg tggacagcca catgcacttc aagagcgcca tccaccccag catcctgcag 5401 aacgggggcc ccatgttcgc cttccgccgc gtggaggagc tgcacagcaa caccgagctg 5461 ggcatcgtgg agtaccagca cgccttcaag acccccatcg ccttcgccag atcccgcgct 5521 cagtcgtcca attctgccgt ggacggcacc gccggacccg gctccaccgg atctcgcgag 5581 ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc tatgaccgag 5641 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc 5701 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc 5761 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag 5821 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa 5881 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg 5941 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg 6001 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc 6061 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc 6121 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag 6181 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg aaatcaacct 6241 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg 6301 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc 6361 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt 6421 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc 6481 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg 6541 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact 6601 gacaattccg tggtgttgtc ggggaaATCA TCGTCCTTTC CTtggctgct cgcctgtgtt 6661 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg 6721 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct Tcgccttcgc 6781 cctcagacga gtcggatctc cctttggGcc gcctccccgc ctggtacctt taagaccaat 6841 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg 6901 gctaattcac tcccaacgaa Gataagatct gctttttgct tgtactgggt ctctctggtt 6961 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca 7021 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa 7081 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg 7141 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa 7201 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 7261 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 7321 tcatgtctgg ctctagctaT CCCGCCCCTA ACTCCGCCCA tcccgcccct aactccgccc 7381 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag 7441 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctagac 7501 ttttgcagag accaaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc 7561 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 7621 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 7681 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 7741 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 7801 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 7861 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 7921 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 7981 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 8041 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 8101 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 8161 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 8221 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 8281 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 8341 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 8401 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 8461 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 8521 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 8581 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 8641 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 8701 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 8761 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 8821 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 8881 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 8941 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 9001 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 9061 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 9121 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 9181 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 9241 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 9301 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 9361 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 9421 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 9481 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 9541 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 9601 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 9661 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa 9721 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct 9781 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag 9841 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc 9901 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg 9961 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga 10021 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc 10081 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc 10141 cagtgccaag ctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.