pLV3-CMV-GRID1-3×FLAG-CopGFP-P

pLV3-CMV-GRID1-3×FLAG-CopGFP-Puro plasmid

PVT96869

pLV3-CMV-GRID1-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-GRID1(human)-3×FLAG-CopGFP-Puro
Alias: NM_017551.3;GluD1; GluD1-b
Gene lenght: 3027bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-GRID1-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-GRID1-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-GRID1-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg aagcgctgac gctgtggctt 2341 ctcccctgga tatgccagtg cgtgtcggtg cgggccgact ccatcatcca catcggtgcc 2401 atcttcgagg agaacgcggc caaggacgac agggtgttcc agttggcggt atccgacctg 2461 agcctcaacg atgacatcct gcagagcgag aagatcacct actccatcaa ggtcatcgag 2521 gccaacaacc cattccaggc tgtgcaggaa gcctgtgacc tcatgaccca ggggattttg 2581 gccttggtca cgtccactgg ctgtgcatct gccaatgccc tgcagtccct cacggatgcc 2641 atgcacatcc cacacctctt tgtccagcgc aacccgggag ggtcgccacg caccgcatgc 2701 cacctgaacc ccagccccga tggtgaggcc tacacactgg cttcgagacc acccgtccgc 2761 ctcaatgatg tcatgctcag gctggtgacg gagctgcgct ggcagaagtt cgtcatgttc 2821 tacgacagcg agtatgatat ccgtgggctt caaagctttc tggaccaggc ctcgcggctg 2881 ggccttgacg tctctttaca aaaggtggac aagaacatta gccacgtatt caccagcctc 2941 ttcaccacga tgaagacaga ggagctgaat cgctaccggg acacgcttcg ccgcgccatc 3001 ctgctgctca gcccacaggg agcccactcc ttcatcaacg aggccgtgga gaccaacctg 3061 gcttccaagg acagccactg ggtctttgtg aatgaggaaa tcagtgaccc ggagatcctg 3121 gatctggtcc atagtgccct tggaaggatg accgtggtcc ggcaaatctt tccgtctgca 3181 aaggacaatc agaaatgcac gaggaacaac caccgcatct cctccctgct ctgcgacccc 3241 caggaaggct acctccagat gctgcagatc tccaacctct atctgtatga cagtgttctg 3301 atgctggcca acgcctttca caggaagctg gaggaccgga agtggcatag catggcgagc 3361 ctcaactgca tacggaaatc cactaagcca tggaatggtg ggaggtccat gctggatacc 3421 atcaaaaagg gccacatcac tggcctcact ggggtgatgg agtttcggga ggacagttcg 3481 aatccctatg tccagtttga aatccttggc actacctata gtgagacttt tggcaaagac 3541 atgcgcaagt tggcgacatg ggactcagag aagggcttga atggcagctt gcaagagagg 3601 cccatgggca gccgcctcca aggattgact cttaaagtgg tgactgtctt ggaagagcct 3661 ttcgtgatgg tggctgagaa catcctagga cagcccaagc gctacaaagg gttctccata 3721 gatgtcctgg atgcactggc caaggctctg ggctttaaat atgagattta ccaagcccct 3781 gatggcaggt acggtcacca gctccataac acctcctgga acgggatgat cggggagctc 3841 atcagcaaga gagcagactt ggccatctct gccatcacca tcaccccaga gagggagagc 3901 gttgtggact tcagcaagcg gtacatggac tattcagtgg ggattctaat taagaagccc 3961 gaggagaaaa tcagcatctt ctccctcttt gctccatttg atttcgctgt gtgggcctgc 4021 attgcagcag ccatccctgt ggttggtgtg ctgatatttg tgttgaacag gatacaggct 4081 gtgagggctc agagtgctgc ccagcccagg ccgtcagctt ctgccactct gcacagcgcc 4141 atctggattg tctatggagc cttcgtacag caaggtggcg aatcttccgt gaactccatg 4201 gccatgcgca tcgtgatggg cagctggtgg ctcttcacgc tcattgtgtg ctcctcctac 4261 acagccaacc ttgctgcctt cctcacagtg tccaggatgg acaaccccat aaggactttc 4321 caggacctgt ccaaacaagt ggaaatgtct tatggcactg tccgggattc tgctgtatat 4381 gagtacttcc gagccaaggg caccaacccc ctggagcagg acagcacgtt tgctgaactc 4441 tggcggacca tcagcaagaa cggaggggct gacaactgcg tgtccagtcc ttcagaaggc 4501 atcaggaagg caaagaaggg gaactacgcc ttcctgtggg atgtggccgt ggtggaatac 4561 gcagccctga cggatgacga ctgctcggtg actgtcatcg gcaacagcat cagcagcaag 4621 ggttacggga ttgccctgca gcatggcagc ccctacaggg acctcttctc ccagaggatc 4681 ctggagctgc aggacacagg ggacctggat gtgctgaagc agaagtggtg gccgcacatg 4741 ggccgctgtg acctcaccag ccatgccagc gcccaggccg acggcaaatc cctcaagctg 4801 cacagcttcg ccggggtctt ctgcatcctg gccattggcc tgctcctggc ctgcctggtg 4861 gctgccctgg agttgtggtg gaacagcaac cggtgccacc aggagacccc caaggaggac 4921 aaagaagtga acttggagca ggtccaccgg cgcatgaaca gcctcatgga tgaagacatt 4981 gctcacaagc agatttcccc agcgtcgatt gagctctcgg ccctggagat ggggggcctg 5041 gctcccaccc agaccttgga gccgacacgg gagtaccaga acacccagct ctcggtcagc 5101 acctttctgc cagagcagag cagccatggc accagccgga cactctcatc agggcccagc 5161 agcaacctgc cgctgccgct gagcagctcg gcgaccatgc cctccatgca gtgcaaacac 5221 aggtcaccca acggggggct gttccggcag agcccggtga agacccccat ccccatgtcc 5281 ttccagcccg tgcctggagg cgtccttcca gaggctctgg acacctccca cgggacctcc 5341 atcggatccG ACTACAAAGA CCATGACGGT GATTATAAAG ATCATGACAT CGACTACAAG 5401 GATGACGATG ACAAGtagcg gccgcGaagg atctgcgatc gctccggtgc ccgtcagtgg 5461 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacg 5521 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc 5581 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt 5641 tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct 5701 tcacgcgccc gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg 5761 cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg 5821 tcgagaccgg gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc 5881 acgctttgcc tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc 5941 cgttacagat ccaagctgtg accggcgcct acgctagacg ccaccatgga gagcgacgag 6001 agcggcctgc ccgccatgga gatcgagtgc cgcatcaccg gcaccctgaa cggcgtggag 6061 ttcgagctgg tgggcggcgg agagggcacc cccaagcagg gccgcatgac caacaagatg 6121 aagagcacca aaggcgccct gaccttcagc ccctacctgc tgagccacgt gatgggctac 6181 ggcttctacc acttcggcac ctaccccagc ggctacgaga accccttcct gcacgccatc 6241 aacaacggcg gctacaccaa cacccgcatc gagaagtacg aggacggcgg cgtgctgcac 6301 gtgagcttca gctaccgcta cgaggccggc cgcgtgatcg gcgacttcaa ggtggtgggc 6361 accggcttcc ccgaggacag cgtgatcttc accgacaaga tcatccgcag caacgccacc 6421 gtggagcacc tgcaccccat gggcgataac gtgctggtgg gcagcttcgc ccgcaccttc 6481 agcctgcgcg acggcggcta ctacagcttc gtggtggaca gccacatgca cttcaagagc 6541 gccatccacc ccagcatcct gcagaacggg ggccccatgt tcgccttccg ccgcgtggag 6601 gagctgcaca gcaacaccga gctgggcatc gtggagtacc agcacgcctt caagaccccc 6661 atcgccttcg ccagatcccg cgctcagtcg tccaattctg ccgtggacgg caccgccgga 6721 cccggctcca ccggatctcg cgagggcaga ggaagtcttc taacatgcgg tgacgtggag 6781 gagaatcccg gccctatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac 6841 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac 6901 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg 6961 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc 7021 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg 7081 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg 7141 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag 7201 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg 7261 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc 7321 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc 7381 aagcccggtg cctgaaatca acctctggat tacaaaattt gtgaaagatt gactggtatt 7441 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat 7501 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct 7561 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct 7621 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc 7681 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg 7741 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa ATCATCGTCC 7801 TTTCCTtggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac 7861 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg 7921 cctcttccgc gtctTcgcct tcgccctcag acgagtcgga tctccctttg gGccgcctcc 7981 ccgcctggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt 8041 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaaGataag atctgctttt 8101 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact 8161 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc 8221 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa 8281 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa 8341 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag 8401 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt 8461 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctaTCCCGCC CCTAACTCCG 8521 CCCAtcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt 8581 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga 8641 ggaggctttt ttggaggcct agacttttgc agagaccaaa ttcgtaatca tgtcatagct 8701 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat 8761 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc 8821 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg 8881 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct 8941 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 9001 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 9061 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 9121 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 9181 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 9241 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 9301 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 9361 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 9421 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 9481 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt 9541 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 9601 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 9661 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 9721 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 9781 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 9841 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 9901 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 9961 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 10021 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 10081 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 10141 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 10201 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 10261 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 10321 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 10381 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 10441 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac 10501 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 10561 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 10621 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 10681 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 10741 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 10801 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat 10861 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg 10921 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg 10981 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg 11041 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat 11101 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc 11161 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca 11221 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca 11281 gtcacgacgt tgtaaaacga cggccagtgc caagctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.