pLV3-U6-FBXO7-shRNA2-CopGFP-Pu

pLV3-U6-FBXO7-shRNA2-CopGFP-Puro plasmid

PVT96796

pLV3-U6-FBXO7-shRNA2-CopGFP-Puro plasmid Information

Plasmid name: pLV3-U6-FBXO7(human)-shRNA2-CopGFP-Puro
Alias: CCGCCTTCCTCCAGGAATTAT
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Interference silence
Fragment type: shRNA
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction:

pLV3-U6-FBXO7-shRNA2-CopGFP-Puro plasmid Description

pLV3-U6-FBXO7-shRNA2-CopGFP-Puro This is a plasmid suitable for interference silencing in mammalian cells, and it can also be used for lentivirus packaging.

pLV3-U6-FBXO7-shRNA2-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtGAGGGCCT ATTTCCCATG ATTCCTTCAT ATTTGCATAT ACGATACAAG 1981 GCTGTTAGAG AGATAATTAG AATTAATTTG ACTGTAAACA CAAAGATATT AGTACAAAAT 2041 ACGTGACGTA GAAAGTAATA ATTTCTTGGG TAGTTTGCAG TTTTAAAATT ATGTTTTAAA 2101 ATGGACTATC ATATGCTTAC CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC 2161 TTGTGGAAAG GACTCATCgc tagcgaattc gCCGCCTTCC TCCAGGAATT ATctcgagAT 2221 AATTCCTGGA GGAAGGCGGt tttttggatc cgcggccgcG aaggatctgc gatcgctccg 2281 gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg 2341 tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg 2401 tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg 2461 ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acagctgaag cttcgagggg 2521 ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg ccgccatcca cgccggttga 2581 gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa 2641 gtttaaagct caggtcgaga ccgggccttt gtccggcgct cccttggagc ctacctagac 2701 tcagccggct ctccacgctt tgcctgaccc tgcttgctca actctacgtc tttgtttcgt 2761 tttctgttct gcgccgttac agatccaagc tgtgaccggc gcctacgcta gacgccacca 2821 tggagagcga cgagagcggc ctgcccgcca tggagatcga gtgccgcatc accggcaccc 2881 tgaacggcgt ggagttcgag ctggtgggcg gcggagaggg cacccccaag cagggccgca 2941 tgaccaacaa gatgaagagc accaaaggcg ccctgacctt cagcccctac ctgctgagcc 3001 acgtgatggg ctacggcttc taccacttcg gcacctaccc cagcggctac gagaacccct 3061 tcctgcacgc catcaacaac ggcggctaca ccaacacccg catcgagaag tacgaggacg 3121 gcggcgtgct gcacgtgagc ttcagctacc gctacgaggc cggccgcgtg atcggcgact 3181 tcaaggtggt gggcaccggc ttccccgagg acagcgtgat cttcaccgac aagatcatcc 3241 gcagcaacgc caccgtggag cacctgcacc ccatgggcga taacgtgctg gtgggcagct 3301 tcgcccgcac cttcagcctg cgcgacggcg gctactacag cttcgtggtg gacagccaca 3361 tgcacttcaa gagcgccatc caccccagca tcctgcagaa cgggggcccc atgttcgcct 3421 tccgccgcgt ggaggagctg cacagcaaca ccgagctggg catcgtggag taccagcacg 3481 ccttcaagac ccccatcgcc ttcgccagat cccgcgctca gtcgtccaat tctgccgtgg 3541 acggcaccgc cggacccggc tccaccggat ctcgcgaggg cagaggaagt cttctaacat 3601 gcggtgacgt ggaggagaat cccggcccta tgaccgagta caagcccacg gtgcgcctcg 3661 ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc 3721 ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag 3781 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg 3841 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga 3901 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag 3961 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct 4021 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg 4081 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct 4141 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct 4201 ggtgcatgac ccgcaagccc ggtgcctgaa atcaacctct ggattacaaa atttgtgaaa 4261 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa 4321 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat 4381 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt 4441 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc 4501 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc 4561 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg 4621 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga 4681 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc 4741 tgccggctct gcggcctctt ccgcgtctcc gccttcgccc tcagacgagt cggatctccc 4801 tttggccgcc tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc 4861 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaaaat 4921 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc 4981 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc 5041 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt 5101 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat 5161 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg 5221 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt 5281 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc 5341 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat 5401 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt 5461 ttttggaggc ctagactttt gcagagacgg cccaaattcg taatcatggt catagctgtt 5521 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa 5581 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact 5641 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc 5701 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg 5761 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 5821 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 5881 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 5941 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 6001 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 6061 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 6121 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 6181 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 6241 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 6301 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt 6361 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 6421 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 6481 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 6541 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 6601 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 6661 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 6721 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 6781 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 6841 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 6901 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 6961 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 7021 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 7081 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 7141 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 7201 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 7261 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 7321 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 7381 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac 7441 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 7501 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 7561 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 7621 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat 7681 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt 7741 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct 7801 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg 7861 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg 7921 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt 7981 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct 8041 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc 8101 acgacgttgt aaaacgacgg ccagtgccaa gctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.