pLV3-CMV-GATA6-3×FLAG-CopGFP-P

pLV3-CMV-GATA6-3×FLAG-CopGFP-Puro plasmid

PVT96852

pLV3-CMV-GATA6-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-GATA6(human)-3×FLAG-CopGFP-Puro
Alias: NM_005257.6;
Gene lenght: 1785bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-GATA6-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-GATA6-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-GATA6-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg ccttgactga cggcggctgg 2341 tgcttgccga agcgcttcgg ggccgcgggt gcggacgcca gcgactccag agcctttcca 2401 gcgcgggagc cctccacgcc gccttccccc atctcttcct cgtcctcctc ctgctcccgg 2461 ggcggagagc ggggccccgg cggcgccagc aactgcggga cgcctcagct cgacacggag 2521 gcggcggccg gacccccggc ccgctcgctg ctgctcagtt cctacgcttc gcatcccttc 2581 ggggctcccc acggaccttc ggcgcctggg gtcgcgggcc ccgggggcaa cctgtcgagc 2641 tgggaggact tgctgctgtt cactgacctc gaccaagccg cgaccgccag caagctgctg 2701 tggtccagcc gcggcgccaa gctgagcccc ttcgcacccg agcagccgga ggagatgtac 2761 cagaccctcg ccgctctctc cagccagggt ccggccgcct acgacggcgc gcccggcggc 2821 ttcgtgcact ctgcggccgc ggcggcagca gccgcggcgg cggccagctc cccggtctac 2881 gtgcccacca cccgcgtggg ttccatgctg cccggcctac cgtaccacct gcaggggtcg 2941 ggcagtgggc cagccaacca cgcgggcggc gcgggcgcgc accccggctg gcctcaggcc 3001 tcggccgaca gccctccata cggcagcgga ggcggcgcgg ctggcggcgg ggccgcgggg 3061 cctggcggcg ctggctcagc cgcggcgcac gtctcggcgc gcttccccta ctctcccagc 3121 ccgcccatgg ccaacggcgc cgcgcgggag ccgggaggct acgcggcggc gggcagtggg 3181 ggcgcgggag gcgtgagcgg cggcggcagt agcctggcgg ccatgggcgg ccgcgagccc 3241 cagtacagct cgctgtcggc cgcgcggccg ctgaacggga cgtaccacca ccaccaccac 3301 caccaccacc accatccgag cccctactcg ccctacgtgg gggcgccact gacgcctgcc 3361 tggcccgccg gacccttcga gaccccggtg ctgcacagcc tgcagagccg cgccggagcc 3421 ccgctcccgg tgccccgggg tcccagtgca gacctgctgg aggacctgtc cgagagccgc 3481 gagtgcgtga actgcggctc catccagacg ccgctgtggc ggcgggacgg caccggccac 3541 tacctgtgca acgcctgcgg gctctacagc aagatgaacg gcctcagccg gcccctcatc 3601 aagccgcaga agcgcgtgcc ttcatcacgg cggcttggat tgtcctgtgc caactgtcac 3661 accacaacta ccaccttatg gcgcagaaac gccgagggtg aacccgtgtg caatgcttgt 3721 ggactctaca tgaaactcca tggggtgccc agaccacttg ctatgaaaaa agagggaatt 3781 caaaccagga aacgaaaacc taagaacata aataaatcaa agacttgctc tggtaatagc 3841 aataattcca ttcccatgac tccaacttcc acctcttcta actcagatga ttgcagcaaa 3901 aatacttccc ccacaacaca acctacagcc tcaggggcgg gtgccccggt gatgactggt 3961 gcgggagaga gcaccaatcc cgagaacagc gagctcaagt attcgggtca agatgggctc 4021 tacataggcg tcagtctcgc ctcgccggcc gaagtcacgt cctccgtgcg accggattcc 4081 tggtgcgccc tggccctggc cggatccGAC TACAAAGACC ATGACGGTGA TTATAAAGAT 4141 CATGACATCG ACTACAAGGA TGACGATGAC AAGtagcggc cgcGaaggat ctgcgatcgc 4201 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga 4261 ggggtcggca attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat 4321 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta 4381 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga 4441 ggggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg 4501 ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag 4561 gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct 4621 agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt 4681 tcgttttctg ttctgcgccg ttacagatcc aagctgtgac cggcgcctac gctagacgcc 4741 accatggaga gcgacgagag cggcctgccc gccatggaga tcgagtgccg catcaccggc 4801 accctgaacg gcgtggagtt cgagctggtg ggcggcggag agggcacccc caagcagggc 4861 cgcatgacca acaagatgaa gagcaccaaa ggcgccctga ccttcagccc ctacctgctg 4921 agccacgtga tgggctacgg cttctaccac ttcggcacct accccagcgg ctacgagaac 4981 cccttcctgc acgccatcaa caacggcggc tacaccaaca cccgcatcga gaagtacgag 5041 gacggcggcg tgctgcacgt gagcttcagc taccgctacg aggccggccg cgtgatcggc 5101 gacttcaagg tggtgggcac cggcttcccc gaggacagcg tgatcttcac cgacaagatc 5161 atccgcagca acgccaccgt ggagcacctg caccccatgg gcgataacgt gctggtgggc 5221 agcttcgccc gcaccttcag cctgcgcgac ggcggctact acagcttcgt ggtggacagc 5281 cacatgcact tcaagagcgc catccacccc agcatcctgc agaacggggg ccccatgttc 5341 gccttccgcc gcgtggagga gctgcacagc aacaccgagc tgggcatcgt ggagtaccag 5401 cacgccttca agacccccat cgccttcgcc agatcccgcg ctcagtcgtc caattctgcc 5461 gtggacggca ccgccggacc cggctccacc ggatctcgcg agggcagagg aagtcttcta 5521 acatgcggtg acgtggagga gaatcccggc cctatgaccg agtacaagcc cacggtgcgc 5581 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac 5641 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg 5701 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac 5761 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc 5821 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg 5881 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc 5941 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag 6001 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc 6061 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc 6121 acctggtgca tgacccgcaa gcccggtgcc tgaaatcaac ctctggatta caaaatttgt 6181 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct 6241 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat 6301 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg 6361 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag 6421 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc 6481 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg 6541 tcggggaaAT CATCGTCCTT TCCTtggctg ctcgcctgtg ttgccacctg gattctgcgc 6601 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc 6661 ctgctgccgg ctctgcggcc tcttccgcgt ctTcgccttc gccctcagac gagtcggatc 6721 tccctttggG ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt 6781 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg 6841 aaGataagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg 6901 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt 6961 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc 7021 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta 7081 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta 7141 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 7201 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct 7261 aTCCCGCCCC TAACTCCGCC CAtcccgccc ctaactccgc ccagttccgc ccattctccg 7321 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag 7381 ctattccaga agtagtgagg aggctttttt ggaggcctag acttttgcag agaccaaatt 7441 cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa 7501 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac 7561 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 7621 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc 7681 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 7741 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 7801 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 7861 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 7921 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 7981 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 8041 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 8101 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 8161 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 8221 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 8281 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 8341 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 8401 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 8461 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 8521 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 8581 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 8641 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 8701 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 8761 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 8821 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 8881 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 8941 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 9001 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 9061 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 9121 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 9181 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 9241 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 9301 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 9361 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 9421 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 9481 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 9541 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 9601 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag 9661 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc 9721 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc 9781 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt 9841 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg 9901 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc 9961 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt 10021 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca agctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.