pLV3-CMV-Gata6-3×FLAG-Fluc-Pur

pLV3-CMV-Gata6-3×FLAG-Fluc-Puro plasmid

PVT95098

pLV3-CMV-Gata6-3×FLAG-Fluc-Puro plasmid Information

Plasmid name: pLV3-CMV-Gata6(mouse)-3×FLAG-Fluc-Puro
Alias: NM_010258;GATA-6
Gene lenght: 1767bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Fluc
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Gata6-3×FLAG-Fluc-Puro plasmid Description

pLV3-CMV-Gata6-3×FLAG-Fluc-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-Gata6-3×FLAG-Fluc-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac 2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag gaattcGCCA 2281 CCatggcctt gactgacggc ggctggtgcc tgccaaagcg tttcggggct gctgctgcgg 2341 acgccggcga ctccgggccc tttccagcgc gggagccctc ctcgccgctt tcccccatct 2401 cgtcttcgtc ctcctcctgc tcccggggcg gggatcgcgg tccctgcggc gccagcaact 2461 gcaggacgcc gcagctcgac gccgaggcgg tggcgggacc tccgggccgc tcgctcttgc 2521 tcagccccta cgcctcgcat cccttcgccg ctgcccacgg agccgcggcg cccggggtcg 2581 caggccccgg gagcgccctg tcgacttggg aggacctgtt gctcttcact gacctcgatc 2641 aggccgcgac cgccagcaag ctgttgtggt ccagccgggg cgccaaactg agccccttcg 2701 cggccgagca gccggaggaa atgtaccaga ccctcgccgc cctgtccagc caggggcccg 2761 ccgcttacga cggcgcgccc ggcggcttcg tgcactccgc agcggcggcg gccgctgccg 2821 ccgcggcagc cagctccccg gtctacgtgc ccaccacgcg cgtgggctcc atgctgtccg 2881 gcctgcccta ccttcaaggg gcgggcagcg ggcccagcaa tcacgcgggc ggagcgggtg 2941 cccacccagg ctggtcccag gcctccgccg acagcccccc gtatggcggg ggtggcgcag 3001 ccggcggcgg cgcggccgga cctggaggtg cgggatcggc tacggcccac gcctctgcac 3061 gctttcccta ctcgcccagc ccgcccatgg ccaacggcgc cgcgcgagac cccgggggct 3121 acgtggctgc gggcggcacg ggcgcaggca gtgtgagtgg aggtggcggc agcctggcgg 3181 ccatgggtgg ccgggagcac cagtacagct cgctgtccgc agctcggccg ctgaacggaa 3241 cgtaccacca ccaccatcac catcacccga cctactcgcc ctacatggcc gcaccgctga 3301 ctcctgcctg gccagcagga cccttcgaaa cgccggtgct ccacagctta cagggccgcg 3361 cgggagctcc actcccggtg ccacggggcc ccagcacaga cctgttggag gacctgtcgg 3421 agagccgcga gtgcgtgaac tgcggctcca tccagacgcc actgtggaga cgagacggca 3481 ccggtcatta cctgtgcaat gcatgcggtc tctacagcaa gatgaatggc ctcagcaggc 3541 ccctcatcaa gccacagaag cgcgtgcctt catcacggcg gcttggactg tcctgtgcca 3601 actgtcacac cacaaccact accttatggc gtagaaatgc tgagggtgag cctgtgtgca 3661 atgcttgcgg gctctatatg aaactccatg gggtgcctcg accacttgct atgaaaaaag 3721 aaggaattca aaccaggaaa cgaaaaccta aaaatataaa taagtcaaaa gcttgctccg 3781 gtaacagcag tggctctgtc cctatgactc ctacttcctc ttcttctaat tcagatgact 3841 gcaccaaaaa tacttctcct tctacacaag cgaccacctc aggggtaggg gcatcagtga 3901 tgtctgcagt gggagaaaac gccaaccccg agaacagtga cctcaagtat tcaggtcaag 3961 acggcctcta cataggtgtc agtctgtcct cccctgccga agtcacatcc tccgtgcgac 4021 aggattcttg gtgtgctctg gccctggccC TCGAGGACTA CAAAGACCAT GACGGTGATT 4081 ATAAAGATCA TGACATCGAC TACAAGGATG ACGATGACAA Gtaaggatcc gcggccgcaa 4141 ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg 4201 agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa 4261 actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt 4321 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac 4381 agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc 4441 gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg 4501 cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc 4561 cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac 4621 tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg tgaccggcgc 4681 ctacgctaga cgccaccatg gagagcgacg agagcggcct gatggaagac gccaaaaaca 4741 taaagaaagg cccggcgcca ttctatccgc tggaagatgg aaccgctgga gagcaactgc 4801 ataaggctat gaagagatac gccctggttc ctggaacaat tgcttttaca gatgcacata 4861 tcgaggtgga catcacttac gctgagtact tcgaaatgtc cgttcggttg gcagaagcta 4921 tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt atgcagtgaa aactctcttc 4981 aattctttat gccggtgttg ggcgcgttat ttatcggagt tgcagttgcg cccgcgaacg 5041 acatttataa tgaacgtgaa ttgctcaaca gtatgggcat ttcgcagcct accgtggtgt 5101 tcgtttccaa aaaggggttg caaaaaattt tgaacgtgca aaaaaagctc ccaatcatcc 5161 aaaaaattat tatcatggat tctaaaacgg attaccaggg atttcagtcg atgtacacgt 5221 tcgtcacatc tcatctacct cccggtttta atgaatacga ttttgtgcca gagtccttcg 5281 atagggacaa gacaattgca ctgatcatga actcctctgg atctactggt ctgcctaaag 5341 gtgtcgctct gcctcataga actgcctgcg tgagattctc gcatgccaga gatcctattt 5401 ttggcaatca aatcattccg gatactgcga ttttaagtgt tgttccattc catcacggtt 5461 ttggaatgtt tactacactc ggatatttga tatgtggatt tcgagtcgtc ttaatgtata 5521 gatttgaaga agagctgttt ctgaggagcc ttcaggatta caagattcaa agtgcgctgc 5581 tggtgccaac cctattctcc ttcttcgcca aaagcactct gattgacaaa tacgatttat 5641 ctaatttaca cgaaattgct tctggtggcg ctcccctctc taaggaagtc ggggaagcgg 5701 ttgccaagag gttccatctg ccaggtatca ggcaaggata tgggctcact gagactacat 5761 cagctattct gattacaccc gagggggatg ataaaccggg cgcggtcggt aaagttgttc 5821 cattttttga agcgaaggtt gtggatctgg ataccgggaa aacgctgggc gttaatcaaa 5881 gaggcgaact gtgtgtgaga ggtcctatga ttatgtccgg ttatgtaaac aatccggaag 5941 cgaccaacgc cttgattgac aaggatggat ggctacattc tggagacata gcttactggg 6001 acgaagacga acacttcttc atcgttgacc gcctgaagtc tctgattaag tacaaaggct 6061 atcaggtggc tcccgctgaa ttggaatcca tcttgctcca acaccccaac atcttcgacg 6121 caggtgtcgc aggtcttccc gacgatgacg ccggtgaact tcccgccgcc gttgttgttt 6181 tggagcacgg aaagacgatg acggaaaaag agatcgtgga ttacgtcgcc agtcaagtaa 6241 caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga cgaagtaccg aaaggtctta 6301 ccggaaaact cgacgcaaga aaaatcagag agatcctcat aaaggccaag aagggcggaa 6361 agatcgccgt gagatcccgc gctcagtcgt ccaattctgc cgtggacggc accgccggac 6421 ccggctccac cggatctcgc gagggcagag gaagtcttct aacatgcggt gacgtggagg 6481 agaatcccgg ccctatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg 6541 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca 6601 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc 6661 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct 6721 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg 6781 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc 6841 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg 6901 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg 6961 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct 7021 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca 7081 agcccggtgc ctgaaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc 7141 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg 7201 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc 7261 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg 7321 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg 7381 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga 7441 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct 7501 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg 7561 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc 7621 ctcttccgcg tctccgcctt cgccctcaga cgagtcggat ctccctttgg ccgcctcccc 7681 gcctggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa 7741 aagaaaaggg gggactggaa gggctaattc actcccaacg aaaataagat ctgctttttg 7801 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag 7861 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc 7921 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa 7981 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat 8041 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca 8101 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt 8161 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc 8221 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga 8281 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctaga 8341 cttttgcaga gacggcccaa attcgtaatc atggtcatag ctgtttcctg tgtgaaattg 8401 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg 8461 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc 8521 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 8581 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 8641 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 8701 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 8761 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 8821 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 8881 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 8941 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 9001 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 9061 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 9121 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 9181 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct 9241 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 9301 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 9361 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 9421 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 9481 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 9541 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 9601 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 9661 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 9721 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 9781 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 9841 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 9901 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 9961 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 10021 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 10081 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 10141 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 10201 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 10261 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 10321 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 10381 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 10441 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 10501 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac 10561 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga 10621 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg 10681 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa 10741 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca 10801 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg 10861 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg 10921 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac 10981 gacggccagt gccaagctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.