pLV3-CMV-SOX8-3×FLAG-CopGFP-Pu

pLV3-CMV-SOX8-3×FLAG-CopGFP-Puro plasmid

PVT96830

pLV3-CMV-SOX8-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-SOX8(human)-3×FLAG-CopGFP-Puro
Alias: NM_014587.5;
Gene lenght: 1338bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-SOX8-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-SOX8-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-SOX8-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgc tggacatgag cgaggcccgc 2341 tcccagccgc cctgcagccc gtccggcacc gccagctcca tgtcgcacgt ggaggactcg 2401 gactcggacg cgccgccgtc tcccgccggc tccgagggcc tgggccgcgc gggggtcgcg 2461 gtggggggcg cccggggcga cccggcggag gcggcggacg agcgcttccc ggcctgcatc 2521 cgcgacgccg tgtcgcaggt gctcaagggc tacgactgga gtctggtgcc catgccggtg 2581 cgcggcggcg gcggcggcgc gctcaaagcc aagccgcatg tgaagcggcc catgaacgca 2641 ttcatggtgt gggcgcaggc ggcgcgccgc aagctggccg accagtaccc gcacctgcac 2701 aacgccgagc tcagcaagac gctgggcaag ctgtggcgct tgctgagcga gagcgagaag 2761 cggcccttcg tggaggaggc agagcgcctt cgcgtgcagc acaagaagga ccaccccgac 2821 tacaagtacc agccacggcg caggaagagc gccaaagccg gccacagcga ctccgactcg 2881 ggcgcggagc tgggacccca ccctggcggc ggtgccgtgt acaaggctga agcagggctt 2941 ggagatgggc accaccatgg cgaccacaca gggcagaccc acgggccgcc caccccgccc 3001 accaccccca agacggagct gcagcaggcg ggcgccaagc cggagctgaa gctggaggga 3061 cgccggccgg tggacagcgg gcgccagaac atcgacttca gcaacgtgga catctcggag 3121 ctcagcagcg aggtcatggg caccatggac gccttcgacg tccacgagtt cgaccagtac 3181 ctgcccctgg gcggccccgc cccacccgag ccgggccagg cctatggggg cgcctacttc 3241 cacgccgggg cgtcccccgt gtgggcccac aagagtgccc cgtcggcctc cgcgtcgccc 3301 accgagacgg gtcccccacg gccgcacatc aagacggagc agccgagccc cggccactac 3361 ggcgaccagc cccgaggctc gcccgactac ggttcctgca gcggccagtc cagcgccacc 3421 ccggccgccc ccgccggccc cttcgccggc tcacagggcg actatggcga cctgcaggcc 3481 tccagctact atggtgccta ccctggctac gcacccggcc tctaccagta cccctgcttc 3541 cactcgccgc gccggcccta cgcctcaccc ctgctcaacg gcctggccct gccgcccgcc 3601 cacagcccca ccagtcactg ggaccagccg gtgtacacca ccctgaccag gcccggatcc 3661 GACTACAAAG ACCATGACGG TGATTATAAA GATCATGACA TCGACTACAA GGATGACGAT 3721 GACAAGtagc ggccgcGaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc 3781 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag 3841 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc 3901 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac 3961 gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc 4021 cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc 4081 tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg 4141 ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc 4201 ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga 4261 tccaagctgt gaccggcgcc tacgctagac gccaccatgg agagcgacga gagcggcctg 4321 cccgccatgg agatcgagtg ccgcatcacc ggcaccctga acggcgtgga gttcgagctg 4381 gtgggcggcg gagagggcac ccccaagcag ggccgcatga ccaacaagat gaagagcacc 4441 aaaggcgccc tgaccttcag cccctacctg ctgagccacg tgatgggcta cggcttctac 4501 cacttcggca cctaccccag cggctacgag aaccccttcc tgcacgccat caacaacggc 4561 ggctacacca acacccgcat cgagaagtac gaggacggcg gcgtgctgca cgtgagcttc 4621 agctaccgct acgaggccgg ccgcgtgatc ggcgacttca aggtggtggg caccggcttc 4681 cccgaggaca gcgtgatctt caccgacaag atcatccgca gcaacgccac cgtggagcac 4741 ctgcacccca tgggcgataa cgtgctggtg ggcagcttcg cccgcacctt cagcctgcgc 4801 gacggcggct actacagctt cgtggtggac agccacatgc acttcaagag cgccatccac 4861 cccagcatcc tgcagaacgg gggccccatg ttcgccttcc gccgcgtgga ggagctgcac 4921 agcaacaccg agctgggcat cgtggagtac cagcacgcct tcaagacccc catcgccttc 4981 gccagatccc gcgctcagtc gtccaattct gccgtggacg gcaccgccgg acccggctcc 5041 accggatctc gcgagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc 5101 ggccctatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg 5161 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat 5221 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg 5281 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg 5341 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg 5401 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc 5461 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt 5521 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc 5581 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc 5641 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt 5701 gcctgaaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat 5761 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct 5821 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag 5881 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc 5941 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc 6001 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct 6061 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aATCATCGTC CTTTCCTtgg 6121 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg 6181 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg 6241 cgtctTcgcc ttcgccctca gacgagtcgg atctcccttt ggGccgcctc cccgcctggt 6301 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa 6361 ggggggactg gaagggctaa ttcactccca acgaaGataa gatctgcttt ttgcttgtac 6421 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc 6481 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt 6541 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag 6601 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc 6661 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat 6721 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact 6781 catcaatgta tcttatcatg tctggctcta gctaTCCCGC CCCTAACTCC GCCCAtcccg 6841 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt 6901 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt 6961 tttggaggcc tagacttttg cagagaccaa attcgtaatc atgtcatagc tgtttcctgt 7021 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa 7081 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc 7141 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag 7201 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 7261 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 7321 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 7381 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 7441 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 7501 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 7561 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 7621 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 7681 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 7741 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 7801 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat 7861 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 7921 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 7981 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 8041 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 8101 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 8161 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 8221 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 8281 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 8341 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 8401 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 8461 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 8521 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 8581 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 8641 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 8701 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 8761 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 8821 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 8881 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 8941 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 9001 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 9061 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 9121 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat 9181 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga 9241 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc 9301 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg 9361 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga 9421 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct 9481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa 9541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg 9601 ttgtaaaacg acggccagtg ccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.