pLV2-CMV-KIF4A-3×FLAG-Puro pla

pLV2-CMV-KIF4A-3×FLAG-Puro plasmid

PVT90328

pLV2-CMV-KIF4A-3×FLAG-Puro plasmid Information

Plasmid name: pLV2-CMV-KIF4A(human)-3×FLAG-Puro
Alias: NM_012310.5;KIF4; KIF4G1; MRX100; TMDI; XLID100;
Gene lenght: 3696bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-KIF4A-3×FLAG-Puro plasmid Description

pLV2-CMV-KIF4A-3×FLAG-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV2-CMV-KIF4A-3×FLAG-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcGCCA CCatgaagga agaggtgaag ggaattcccg taagagtggc 2881 gctgcgttgt cgccctctgg tccccaaaga gattagcgag ggctgccaga tgtgcctttc 2941 cttcgtgccc ggagagcctc aggtggtggt tggtacagat aaatccttca cctacgattt 3001 tgtatttgat ccctctactg aacaggaaga agtcttcaat acagcagtag cgccactcat 3061 aaaaggtgta tttaaaggat ataatgcaac ggtcctggcc tatgggcaga ctggctctgg 3121 aaaaacctat tcaatgggag gtgcatatac tgcagagcaa gagaatgaac caacagttgg 3181 ggttattcct agggtaatac aactgctctt caaagaaatt gataaaaaga gtgactttga 3241 atttactctg aaagtgtctt acttagagat ttacaatgaa gaaattttgg atcttctatg 3301 cccatctcgt gagaaagctc aaataaatat acgagaggat cctaaggaag gcataaagat 3361 tgtgggactc actgagaaga ctgttttggt tgccttggat actgtttcct gtttggaaca 3421 gggcaacaac tctaggactg tggcctccac ggctatgaac tcccagtcgt cccgatctca 3481 tgccatcttt acaatctcct tagagcaaag aaagaaaagt gacaagaata gcagctttcg 3541 ctccaagctg catcttgtag acctcgctgg atcagaaaga cagaagaaaa ccaaggctga 3601 aggggatcgt ctaaaagagg gtattaatat taaccgaggc ctcctatgct tgggaaatgt 3661 aatcagtgct cttggagatg acaaaaaggg tggctttgtg ccctacagag attccaagtt 3721 gactcgactg cttcaagatt ctctaggagg taatagccat actcttatga tagcctgtgt 3781 gagtcctgct gactccaatc tagaggaaac attaaatacc cttcgctatg ctgacagagc 3841 aagaaaaatc aagaacaaac ctattgttaa tattgatccc cagacagctg aacttaatca 3901 tctaaagcaa caggtacaac agctacaagt cttgttgcta caggcccatg gaggtaccct 3961 gcctggatct ataactgtgg aaccatcaga gaatctacaa tccctgatgg agaagaatca 4021 gtccctggta gaggagaatg aaaaattaag tcgtggtctg agcgaggcag ctggtcagac 4081 agcccagatg ttggagagga tcattttgac agagcaagcg aatgaaaaaa tgaacgccaa 4141 gctagaagag ctcaggcagc atgcggcctg caaactggat cttcaaaagc tagtggagac 4201 tttggaagac caggaattga aagaaaatgt agagataatt tgtaacctgc agcaattgat 4261 tacccagtta tcggatgaaa ctgttgcttg catggctgca gccattgata ctgcggtgga 4321 gcaagaagcc caagtagaaa ccagtccaga gacgagcagg tcttctgacg cttttaccac 4381 tcagcatgct ctccgtcaag cgcagatgtc taaggagctg gttgagttga ataaagcgct 4441 tgcactgaaa gaggccctgg ctaggaagat gactcagaat gacagccaac tgcagcccat 4501 tcagtaccaa taccaggata acataaaaga gctagaatta gaagtcatca atctgcaaaa 4561 ggaaaaggaa gaattggttc ttgaacttca gacagcaaag aaggatgcca accaagccaa 4621 gttgagtgag cgccgccgca aacgtctcca ggagctggag ggtcaaattg ctgatctgaa 4681 gaagaaactg aatgagcagt ccaaacttct gaaactaaag gaatccacag agcgtactgt 4741 ctccaaactg aaccaggaga tacggatgat gaaaaaccag cgggtacagt taatgcgtca 4801 aatgaaagaa gatgctgaga agtttagaca gtggaagcag aaaaaagaca aagaagtaat 4861 acagttaaaa gaacgagacc gtaagaggca atatgagctg ctgaaacttg aaagaaactt 4921 ccagaaacaa tccaatgtgc tcagacgtaa aacggaggag gcagcagctg ccaacaagcg 4981 tctcaaggat gctctccaga aacaacggga ggttgcagat aagcggaaag agactcagag 5041 ccgtggaatg gaaggcactg cagctcgagt gaagaattgg cttggaaacg aaattgaggt 5101 tatggtcagt actgaggaag ccaaacgcca tctgaatgac ctccttgaag atagaaagat 5161 cctggctcaa gatgtggctc aactcaaaga aaaaaaggaa tctggggaga atccacctcc 5221 taaactccgg aggcgtacat tctcccttac tgaagtgcgt ggtcaagttt cggagtcaga 5281 agattctatt acaaagcaga ttgaaagcct agagactgaa atggaattca ggagtgctca 5341 gattgctgac ctacagcaga agctgctgga tgcagaaagt gaagacagac caaaacaacg 5401 ctgggagaat attgccacca ttctggaagc caagtgtgcc ctgaaatatt tgattggaga 5461 gctggtctcc tccaaaatac aggtcagcaa acttgaaagc agcctgaaac agagcaagac 5521 cagctgtgct gacatgcaga agatgctgtt tgaggaacga aatcattttg ccgagataga 5581 gacagagtta caagctgagc tggtcagaat ggagcaacag caccaagaga aggtgctgta 5641 ccttctcagc cagctgcagc aaagccaaat ggcagagaag cagttagagg aatcagtcag 5701 tgaaaaggaa cagcagctgc tgagcacact gaagtgtcag gatgaagaac ttgagaaaat 5761 gcgagaagtg tgtgagcaaa atcagcagct tctccgagag aatgaaatca tcaagcagaa 5821 actgaccctc ctccaggtag ccagcagaca gaaacatctt cctaaggata cccttctatc 5881 tccagactct tcttttgaat atgtcccacc taagccaaaa ccttctcgtg ttaaagaaaa 5941 gttcctggag caaagcatgg acatcgagga tctaaaatat tgttcagagc attctgtgaa 6001 tgagcatgag gatggtgatg gtgatgatga tgagggggat gacgaggaat ggaagccaac 6061 aaaattagtt aaggtgtcca ggaagaacat ccaagggtgt tcctgcaagg gctggtgtgg 6121 aaacaagcag tgtgggtgca ggaagcaaaa gtcagactgt ggtgtggact gttgctgtga 6181 ccccacaaag tgtcggaacc gccagcaagg caaggatagc ttgggcactg ttgaacggac 6241 ccaggattcc gaaggctcct tcaaactgga ggatcctacc gaggtgaccc caggattgag 6301 cttctttaat cccgtctgtg ccacccccaa tagcaagatc ctgaaagaga tgtgcgatgt 6361 ggagcaggtg ctgtcaaaga agactccccc agctccctcc ccttttgacc tcccagagtt 6421 gaaacatgta gcaacagaat accaagaaaa caaggctcca gggaagaaaa agaaacgggc 6481 tctggccagc aacaccagct tcttctctgg ctgctcccct atcgaagaag aggcccacGG 6541 TGGAGGCgga tccGACTACA AAGACCATGA CGGTGATTAT AAAGATCATG ACATCGACTA 6601 CAAGGATGAC GATGACAAGt agtctagata attctaccgg gtaggggagg cgcttttccc 6661 aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg 6721 cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg 6781 tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca 6841 gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat 6901 ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct 6961 ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag 7021 gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac 7081 gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac 7141 ctgcagccca agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac 7201 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc 7261 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc 7321 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg 7381 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc 7441 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg 7501 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac 7561 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc 7621 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc 7681 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc 7741 cgcaagcccg gtgcctgacc gcgtctggaa caatcaacct ctggattaca aaatttgtga 7801 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt 7861 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa 7921 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt 7981 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct 8041 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg 8101 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc 8161 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg 8221 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct 8281 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc 8341 cctttgggcc gcctccccgc ctggaattaa ttctgcagtc gagacctaga aaaacatgga 8401 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa 8461 gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac 8521 aaggcagctg tagatcttag ccacttttta aaagaaaaga ggggactgga agggctaatt 8581 cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc 8641 cctgattagc agaactacac accagggcca ggggtcagat atccactgac ctttggatgg 8701 tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac 8761 accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta 8821 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag 8881 tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca 8941 gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca 9001 gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc 9061 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt 9121 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc 9181 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact 9241 tgcaaagaaa tgaatatcag agagtgagag gccttgacat tgctagcgtt ttaccgtcga 9301 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 9361 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct 9421 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 9481 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 9541 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 9601 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 9661 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 9721 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 9781 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 9841 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 9901 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 9961 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 10021 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 10081 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 10141 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga 10201 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 10261 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 10321 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 10381 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 10441 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 10501 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 10561 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 10621 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 10681 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 10741 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 10801 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 10861 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 10921 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 10981 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 11041 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 11101 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 11161 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 11221 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 11281 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 11341 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 11401 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 11461 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcaacttgtt tattgcagct 11521 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca 11581 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac 11641 tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact 11701 gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt 11761 taaagaaatt gtatttgtta aatatgtact acaaacttag tagtttttaa agaaattgta 11821 tttgttaaat atgtactaca aacttagtag t

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.