pLV3-CMV-TOX3-3×FLAG-CopGFP-Pu

pLV3-CMV-TOX3-3×FLAG-CopGFP-Puro plasmid

PVT96825

pLV3-CMV-TOX3-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-TOX3(human)-3×FLAG-CopGFP-Puro
Alias: NM_001080430.4;CAGF9; TNRC9
Gene lenght: 1728bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-TOX3-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-TOX3-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-TOX3-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg atgtgaggtt ctaccccgcg 2341 gcggccgggg accctgccag cctggacttc gcgcagtgcc tggggtacta cggctacagc 2401 aagtttggaa ataataataa ctatatgaat atggctgagg cgaacaatgc gttcttcgct 2461 gccagtgagc agacattcca cacaccaagc cttggggacg aggaattcga aattccacca 2521 atcacgcctc ctccagagtc agaccctgcc ctaggcatgc cggatgtact gctacccttt 2581 caagccctca gcgatccatt gccttcccag ggaagtgaat tcacacccca gtttccccct 2641 caaagcctgg acctcccttc cattacaatc tcaagaaatc tcgtggaaca agatggcgtg 2701 cttcatagca gtgggttgca tatggatcag agccacacac aagtgtccca gtaccggcag 2761 gatccctccc tgatcatgcg gtccatcgtc cacatgaccg atgctgcgcg ttctggggtc 2821 atgcctcctg cccagctcac caccatcaac cagtctcagc tcagcgccca gttggggttg 2881 aatttgggag gtgccagtat gcctcacaca tctccttcac ctccagcaag caaatcagcc 2941 actccctccc cttccagctc catcaatgaa gaggatgctg atgaagccaa cagagccatt 3001 ggagagaaaa gagctgctcc agactctggc aagaagccca agactccaaa gaaaaagaaa 3061 aagaaagatc ccaatgagcc acagaagcca gtgtcagcat atgccctgtt tttcagagac 3121 acacaggctg caattaaagg tcaaaacccc aatgcaacct ttggagaggt ctcaaaaatt 3181 gtagcatcta tgtgggacag ccttggagaa gaacaaaagc aggtatataa aaggaaaaca 3241 gaagctgcca aaaaagaata cctgaaggcc ctggcggcat acagggccag cctcgtttct 3301 aaggctgctg ctgagtcagc agaagcccag accatccgtt ctgttcagca gaccctggcg 3361 tcgaccaatc taacatcctc tctccttctc aacactccac tgtctcaaca tggaacagtg 3421 tcagcatcac ctcagactct ccagcaatcc ctccctaggt caatcgctcc caaaccctta 3481 accatgagac tccccatgaa ccagattgtc acatcagtca ccattgcagc caacatgccc 3541 tcgaacattg gggctccact gataagctcc atgggaacga ccatggttgg ctcagcaccc 3601 tccacccaag tgagtccttc ggtgcaaacc cagcagcatc agatgcaatt gcagcagcag 3661 cagcagcagc aacaacaaca gatgcaacag atgcagcagc agcaactcca gcagcaccaa 3721 atgcatcagc aaatccagca gcagatgcag cagcagcatt tccagcacca catgcagcag 3781 cacctgcagc agcagcagca gcatctccag cagcaaatta atcaacagca gctgcagcag 3841 cagctgcagc agcgcctcca gctgcagcag ctgcaacaca tgcagcacca gtctcagcct 3901 tctcctcggc agcactcccc tgtcgcctct cagataacat cccccatccc tgccatcggg 3961 agcccccagc cagcctctca gcagcaccag tcgcaaatac agtctcagac acagactcaa 4021 gtattatcgc aggtcagtat tttcggatcc GACTACAAAG ACCATGACGG TGATTATAAA 4081 GATCATGACA TCGACTACAA GGATGACGAT GACAAGtagc ggccgcGaag gatctgcgat 4141 cgctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg 4201 ggaggggtcg gcaattgaac gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt 4261 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca 4321 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca gctgaagctt 4381 cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc 4441 cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc 4501 taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta 4561 cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt 4621 gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc tacgctagac 4681 gccaccatgg agagcgacga gagcggcctg cccgccatgg agatcgagtg ccgcatcacc 4741 ggcaccctga acggcgtgga gttcgagctg gtgggcggcg gagagggcac ccccaagcag 4801 ggccgcatga ccaacaagat gaagagcacc aaaggcgccc tgaccttcag cccctacctg 4861 ctgagccacg tgatgggcta cggcttctac cacttcggca cctaccccag cggctacgag 4921 aaccccttcc tgcacgccat caacaacggc ggctacacca acacccgcat cgagaagtac 4981 gaggacggcg gcgtgctgca cgtgagcttc agctaccgct acgaggccgg ccgcgtgatc 5041 ggcgacttca aggtggtggg caccggcttc cccgaggaca gcgtgatctt caccgacaag 5101 atcatccgca gcaacgccac cgtggagcac ctgcacccca tgggcgataa cgtgctggtg 5161 ggcagcttcg cccgcacctt cagcctgcgc gacggcggct actacagctt cgtggtggac 5221 agccacatgc acttcaagag cgccatccac cccagcatcc tgcagaacgg gggccccatg 5281 ttcgccttcc gccgcgtgga ggagctgcac agcaacaccg agctgggcat cgtggagtac 5341 cagcacgcct tcaagacccc catcgccttc gccagatccc gcgctcagtc gtccaattct 5401 gccgtggacg gcaccgccgg acccggctcc accggatctc gcgagggcag aggaagtctt 5461 ctaacatgcg gtgacgtgga ggagaatccc ggccctatga ccgagtacaa gcccacggtg 5521 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc 5581 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag 5641 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac 5701 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc 5761 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag 5821 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc 5881 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg 5941 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc 6001 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg 6061 cgcacctggt gcatgacccg caagcccggt gcctgaaatc aacctctgga ttacaaaatt 6121 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct 6181 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg 6241 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc 6301 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt 6361 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc 6421 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg 6481 ttgtcgggga aATCATCGTC CTTTCCTtgg ctgctcgcct gtgttgccac ctggattctg 6541 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc 6601 ggcctgctgc cggctctgcg gcctcttccg cgtctTcgcc ttcgccctca gacgagtcgg 6661 atctcccttt ggGccgcctc cccgcctggt acctttaaga ccaatgactt acaaggcagc 6721 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca 6781 acgaaGataa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc 6841 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg 6901 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag 6961 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca 7021 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc 7081 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 7141 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta 7201 gctaTCCCGC CCCTAACTCC GCCCAtcccg cccctaactc cgcccagttc cgcccattct 7261 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct 7321 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagacttttg cagagaccaa 7381 attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 7441 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact 7501 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct 7561 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 7621 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 7681 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 7741 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 7801 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 7861 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 7921 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 7981 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 8041 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 8101 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 8161 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 8221 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 8281 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 8341 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 8401 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 8461 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 8521 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 8581 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 8641 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 8701 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 8761 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 8821 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 8881 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 8941 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 9001 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 9061 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 9121 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 9181 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 9241 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 9301 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 9361 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 9421 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 9481 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 9541 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac 9601 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct 9661 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg 9721 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat 9781 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata 9841 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg 9901 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg 9961 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.