pCMV-EGFP-Col3a1-Neo plasmid

pCMV-EGFP-Col3a1-Neo plasmid

PVT96816

pCMV-EGFP-Col3a1-Neo plasmid Information

Plasmid name: pCMV-EGFP-Col3a1(mouse)-Neo
Alias: NM_009930.2;Col3a-1; Tsk-2; Tsk2
Gene lenght: 4395bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: pEGFP-C-5'
Reverse primer:
Copy number: High
Induction:

pCMV-EGFP-Col3a1-Neo plasmid Description

pCMV-EGFP-Col3a1-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-EGFP-Col3a1-Neo plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAGCgcc accatggtga gcaagggcga ggagctgttc 901 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc 961 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc 1021 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg 1081 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg 1141 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc 1201 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc 1261 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac 1321 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc 1381 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc 1441 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc 1501 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg 1561 atcactctcg gcatggacga gctgtacaag gGaGGCGGAG GTAGTGGAGG AGGGGGAAGC 1621 ggaggcggag ggagtGAATT Catgatgagc tttgtgcaaa gtggaacctg gtttcttctc 1681 acccttcttc atcccactct tattttggca cagcagtcca acgtagatga attgggatgc 1741 agccaccttg gtcagtccta tgagtctaga gatgtctgga agccagaacc atgtcaaata 1801 tgtgtctgtg actcaggatc tgtcctttgc gatgacataa tctgtgatga ggagccacta 1861 gactgcccca acccagagat cccatttgga gaatgttgtg caatatgccc acagccttct 1921 acacctgctc ctgtgcttcc tgatggccat ggacctcaag gccccaaggg agatccgggt 1981 cctcctggca ttcctgggag aaatggtgac cctggccttc caggacaacc aggtctccct 2041 gggcctcctg gctcccctgg aatctgtgaa tcatgtccaa ctggtggcca aaattattct 2101 ccccaattcg actcatatga tgtcaagtct ggagtgggag gaatgggtgg ctatccggga 2161 ccagctggtc caccaggccc tccaggaccc cctggttctt ctggacatcc tggatctcct 2221 ggttctcctg gataccaagg tccccctggt gaacctggtc aagctggtcc ggcaggccct 2281 ccaggacctc ctggtgctct tggtccagct ggtcctgctg gaaaggatgg agagtcagga 2341 agacctggac gacctggaga gcgtggactg cctggacctc caggtatcaa aggcccagct 2401 ggcatgcctg gattccctgg tatgaaagga cacagaggct ttgatggacg caatggagaa 2461 aagggagaaa caggtgctcc tggactgaag ggtgaaaatg gtcttccagg agacaacgga 2521 gctcctggcc ccatgggtcc tagaggggct cctggtgagc gaggacgacc aggccttcct 2581 ggagctgcag gtgctcgagg caatgatggt gctcggggca gtgatgggca acctggtccc 2641 cctggccctc ctggaactgc aggattccct ggatcccctg gtgctaaggg tgaagttgga 2701 cctgcagggt cccctggctc aaatggctca ccaggacaaa gaggggagcc tggaccgcag 2761 ggacatgctg gtgctcaagg gcctcctggc cctcccggga ataacggcag tcctggtgga 2821 aagggtgaaa tgggtccagc tggcattcct ggagcccctg gactaatagg agcaaggggc 2881 ccccctggac cagcaggaac taatggtata cccggaacac gaggtccttc aggtgaaccc 2941 ggcaagaacg gtgcgaaggg agagccagga gcacgcggtg aacggggcga agctggttcc 3001 ccaggaattc caggacctaa gggcgaagat ggcaaagatg gatcacctgg agaacctggt 3061 gcaaatgggc ttccaggagc cgcaggagaa aggggtccta gtggcttccg aggacctgca 3121 gggccaaatg gcatcccagg agaaaagggt cctcccggag agcgtggtgg cccaggtcct 3181 gcaggaccca gaggagtagc tggagaacct ggccgagatg gaacccctgg aggtccaggg 3241 atacggggta tgcccggaag tccaggtgga ccaggcaatg atggaaaacc aggacctcct 3301 ggaagtcaag gagaaagtgg tcgccctggt ccccctggcc catctggtcc cagaggtcag 3361 cctggtgtca tgggtttccc tggtcctaaa ggaaatgatg gtgcccctgg caagaatgga 3421 gaacggggtg gccctggagg acctggcctt ccaggtcctg ctggaaagaa tggggagact 3481 ggacctcagg gtcccccagg acctactggc ccagctggtg acaagggaga ttctggaccc 3541 cctggtccac aaggattaca aggcatacct ggtaccggtg gtcctccagg agaaaatggg 3601 aaaccaggtg aaccaggtcc aaagggtgaa gtcggtgctc ctggagctcc tggaggcaag 3661 ggtgattctg gtgcacctgg agaacgtggg cctcctggaa cagcaggaat ccctggtgct 3721 agaggtggag ctggaccccc tggccctgag ggaggaaagg gccctgctgg tccccctggc 3781 cctcctggtg cttctggttc tcctggtctg caagggatgc caggggaaag aggaggtcct 3841 ggaagtcctg gcccaaaggg tgaaaagggt gaaccaggtg gtgcaggtgc tgacggagtt 3901 ccaggaaagg atgggccaag gggtcctgct ggccctattg gtccccctgg cccagctggt 3961 cagcctggag ataagggtga aggtggttcc cctggacttc caggtatagc tggacctcga 4021 ggtggccctg gtgagagagg tgaacatggc cctccaggac ctgctggctt cccgggtgct 4081 cctggacaga atggtgaacc aggtgctaaa ggagaaagag gtgcccctgg agagaaaggt 4141 gaaggaggcc ctccaggacc tgcaggaccc actggcagtt ctggacctgc tggtccgcct 4201 ggtcctcagg gtgtaaaggg tgaacgtggt agtcctggtg gtcctggtac tgctggcttt 4261 cccggtgggc gtggtcttcc tggtcctcct ggcaataatg gtaatccagg gcccccagga 4321 cccagtggtg ctcctggcaa ggacggccct ccaggtcctg caggcaacag tggttctcct 4381 ggcaaccctg gaatagctgg accaaaaggt gatgctggac agcctggaga gaaggggcca 4441 cctggtgctc agggtcctcc gggttcccca ggcccacttg gaattgcagg gctaactgga 4501 gcacgaggtc ttgctggacc accaggcatg ccaggtccac ggggtagccc tggacctcag 4561 ggtatcaagg gtgaaagtgg aaaaccagga gccagtggcc ataatgggga acgtggtcct 4621 cctggacccc aaggtcttcc tggtcagcct ggtacagctg gtgaacctgg aagggatgga 4681 aaccctggat cagatggtca gccaggtcga gatggatctc ctggtggcaa gggtgatcgt 4741 ggtgaaaatg gttctcctgg tgccccaggc gctcctggtc atccaggccc acctggccct 4801 gttggtccat ctggaaagag tggtgacaga ggagaaactg gccctgctgg tccttctggt 4861 gctccaggcc ctgctggagc tcgaggtgct ccgggtcccc aaggtccacg aggtgacaaa 4921 ggtgaaactg gtgaacgtgg ctctaatggc atcaaaggac atcgaggatt ccctggcaat 4981 ccaggtcctc caggttctcc tggtgctgct ggtcaccagg gtgcaattgg tagtccagga 5041 cctgcaggtc ccagaggacc agttggacca catggacctc ctggaaaaga tggaacaagt 5101 gggcatccag gtcctattgg accaccaggt cctagaggaa acagaggtga aagaggatct 5161 gagggctcgc caggccaccc tggacagcca ggaccccctg gaccccctgg tgcccctggt 5221 ccctgctgtg gtggtggtgc tgctgccatt gctggagttg gaggtgaaaa gtctggtggc 5281 ttttcaccct attatggaga cgatccaatg gatttcaaga tcaacactga agagattatg 5341 tcttcactca agtctgttaa tggacaaata gagagtctta taagccctga tggttctcga 5401 aaaaaccctg ctcggaactg cagagaccta aaattctgcc accccgaact caagagtgga 5461 gaatactggg ttgatcctaa ccaaggctgc aagatggatg ctataaaagt attctgtaac 5521 atggaaactg gggaaacatg cataaatgcc agccccatga ctgtcccacg taagcactgg 5581 tggacagatt ctggtgcaga gaagaaacat gtttggtttg gagaatctat gaatggtggt 5641 tttcagttca gctatggccc tcctgatctt cctgaagatg tcgttgatgt gcagctggca 5701 ttcctcagac ttctttccag ccgggcctcc cagaacatta cataccactg caaaaacagt 5761 attgcctata tggatcaggc cagtggcaat gtaaagaagt ctctgaagct gatgggatca 5821 aatgaaggcg aattcaaggc tgaaggaaac agcaaattca cttacacagt tctagaggat 5881 ggctgtacta aacacactgg ggaatggagc aagacagtct ttgaatatca aacacgcaag 5941 gcaatgagac tacccatcat agatatcgca ccctatgaca ttgggggtcc tgatcaagaa 6001 tttggtgtgg acattggccc tgtttgcttt ttataaGGAT CCtGATATCC AGCACAGTGG 6061 CGGCCGCTCG AGTCTAGAGG GCCCGTTTAA ACCCGCTGAT CAGCCTCGAC TGTGCCTTCT 6121 AGTTGCCAGC CATCTGTTGT TTGCCCCTCC CCCGTGCCTT CCTTGACCCT GGAAGGTGCC 6181 ACTCCCACTG TCCTTTCCTA ATAAAATGAG GAAATTGCAT CGCATTGTCT GAGTAGGTGT 6241 CATTCTATTC TGGGGGGTGG GGTGGGGCAG GACAGCAAGG GGGAGGATTG GGAAGACAAT 6301 AGCAGGCATG CTGGGGATGC GGTGGGCTCT ATGGCTTCTG AGGCGGAAAG AACCAGCTGG 6361 GGCTCTAGGG GGTATCCCCA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 6421 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 6481 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 6541 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 6601 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 6661 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 6721 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 6781 CTGATTTAAC AAAAATTTAA CGCGAATTAA TTCTGTGGAA TGTGTGTCAG TTAGGGTGTG 6841 GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG 6901 CAACCAGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC 6961 TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC 7021 CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG 7081 AGGCCGCCTC TGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG 7141 GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG 7201 GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT 7261 GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG 7321 CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG 7381 GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG 7441 TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG 7501 GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA 7561 TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC 7621 ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC 7681 AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA 7741 AGGCGCGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA 7801 ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG 7861 CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG 7921 AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG 7981 CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA 8041 CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG 8101 GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT 8161 CATGCTGGAG TTCTTCGCCC ACCCCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA 8221 AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG 8281 TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGTATA CCGTCGACCT CTAGCTAGAG 8341 CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC 8401 ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA 8461 ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA 8521 GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC 8581 CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC 8641 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 8701 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 8761 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 8821 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 8881 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 8941 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 9001 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 9061 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 9121 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 9181 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 9241 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTTTTTT 9301 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 9361 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 9421 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 9481 CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC 9541 TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT 9601 AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC 9661 ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG 9721 AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG 9781 AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT 9841 GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG 9901 AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT 9961 TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC 10021 TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC 10081 ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA 10141 TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG 10201 AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC 10261 CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG 10321 GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT 10381 CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT 10441 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC 10501 ACCTGACGTC GACGGATCGG GAGATCTCCC GATCCCCTAT GGT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.