pLV3-CMV-B3gnt3-6×His-mCherry-

pLV3-CMV-B3gnt3-6×His-mCherry-Puro plasmid

PVT96811

pLV3-CMV-B3gnt3-6×His-mCherry-Puro plasmid Information

Plasmid name: pLV3-CMV-B3gnt3(mouse)-6×His-mCherry-Puro
Alias: NM_028189.3;2210008L19Rik
Gene lenght: 1116bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-B3gnt3-6×His-mCherry-Puro plasmid Description

pLV3-CMV-B3gnt3-6×His-mCherry-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-B3gnt3-6×His-mCherry-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcgcca ccatgcggct gccaaggcag agcccctacg agatcctcct 2341 cctggtcttg gtcgccttgc tggtgctgct gctgctcctg accagcaagt caccgcccag 2401 ctgctctgcc cctgagaggt ccaaggagcc tgaagacaac cccgggtggg ccacgggcca 2461 cccacgcaca caccccgccc ggtgccgagc taatctatcc gtgtcctcgc accccgactt 2521 cgcggggctg cccttacacg tgcgcgactt cttgttctac cgccactgcc gcgacttccc 2581 agtgctccga gagccgcggg ttaccaagtg cgcggagccc gtgttcctgc tgctcgccat 2641 caagtcctcg cctgcaaact atgggcgccg ccagatgctg cgcacgacgt gggcgcgcga 2701 gagacgggtg cgtggggcgc cactgcgccg cctcttcctt gtgggctcag accgcgaccc 2761 acaacaagca cgcaaataca accgactgct ggagctggaa gcgcagaaat acggcgacat 2821 tctccagtgg gatttccatg actccttctt taacctgacg cttaagcagg tccttttcct 2881 ggagtggcag ctaacctact gtaccaacgc cagcttcgtg ctcaatgggg acgacgatgt 2941 gttcgcacac acggacaaca tggtcaccta cctgcaggac cacgacccgg accaacacct 3001 cttcgtgggg cacctgatcc agaacgtggg tcccatccgg gtgccctgga gcaagtactt 3061 catccccgct ctggtgatgg cggaggacag atacccgccc tactgtggtg gcggcggctt 3121 cctgctgtcg cgttttaccg tggccgccct acgtcgcgcc gcgcgcgtcc tccccatgtt 3181 cccaatcgac gacgtgttcc tgggcatgtg tctgcagcag cagggtctgg ctcccgggac 3241 gcacagcgga gtgcgcactg cgggggtttt cccccctagc ccacgtgtgt catccttcga 3301 cccctgcttc taccgcgacc tgctcctcgt gcaccgcttc ctgcccttcg agatgctgct 3361 gatgtgggat gcgctgaacc agccccagct cctctgcggc aggcagagcc ccgcctacCA 3421 TCACCATCAC CATCACTAGg gatccgcggc cgcGaaggat ctgcgatcgc tccggtgccc 3481 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca 3541 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact 3601 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga 3661 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca 3721 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg 3781 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa 3841 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc 3901 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg 3961 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac gctagacgcc accatggtga 4021 gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca 4081 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct 4141 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct 4201 gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg 4261 acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga 4321 acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt 4381 tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga 4441 agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga 4501 agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca 4561 agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca 4621 agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg 4681 agggccgcca ctccaccggc ggcatggacg agctgtacaa gggatctcgc gagggcagag 4741 gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctatgacc gagtacaagc 4801 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg 4861 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg 4921 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg 4981 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg 5041 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc 5101 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg 5161 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc 5221 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc 5281 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg 5341 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaaatcaa cctctggatt 5401 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg 5461 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct 5521 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc 5581 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca 5641 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac 5701 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt 5761 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct 5821 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc 5881 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tctccgcctt cgccctcaga 5941 cgagtcggat ctccctttgg ccgcctcccc gcctggtacc tttaagacca atgacttaca 6001 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc 6061 actcccaacg aaaataagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga 6121 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct 6181 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat 6241 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta 6301 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta 6361 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat 6421 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct 6481 ggctctagct atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg 6541 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa 6601 gtagtgagga ggcttttttg gaggcctaga cttttgcaga gacggcccaa attcgtaatc 6661 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg 6721 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat 6781 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg 6841 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct 6901 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 6961 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 7021 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 7081 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 7141 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 7201 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 7261 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 7321 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 7381 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 7441 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 7501 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 7561 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 7621 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 7681 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 7741 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 7801 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 7861 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 7921 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 7981 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 8041 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 8101 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg 8161 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 8221 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 8281 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 8341 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 8401 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc 8461 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 8521 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 8581 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 8641 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 8701 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 8761 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt 8821 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt 8881 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac 8941 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc 9001 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag 9061 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag 9121 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc 9181 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc 9241 agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.