pCMV-EGFP-USP25-Neo plasmid

pCMV-EGFP-USP25-Neo plasmid

PVT94024

pCMV-EGFP-USP25-Neo plasmid Information

Plasmid name: pCMV-EGFP-USP25(human)-Neo
Alias: NM_013396.6;EIG19; USP21
Gene lenght: 3168bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: BGH-R
Copy number: High
Induction:

pCMV-EGFP-USP25-Neo plasmid Description

pCMV-EGFP-USP25-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-EGFP-USP25-Neo plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAGCgcc accatggtga gcaagggcga ggagctgttc 901 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc 961 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc 1021 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg 1081 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg 1141 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc 1201 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc 1261 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac 1321 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc 1381 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc 1441 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc 1501 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg 1561 atcactctcg gcatggacga gctgtacaag gGaGGCGGAG GTAGTGGAGG AGGGGGAAGC 1621 ggaggcggag ggagtGAATT Catgaccgtg gagcagaacg tgctgcagca gagcgcggcg 1681 cagaagcacc agcagacgtt tttgaatcaa ctgagagaaa ttacggggat taatgacacc 1741 cagatactac agcaagcctt gaaggatagt aatggaaact tggaattagc agtggctttc 1801 cttactgcga agaatgctaa gacccctcag caggaggaga caacttacta ccaaacagca 1861 cttcctggca atgatagata catcagtgtg ggaagccaag cagatacaaa tgtgattgat 1921 ctcactggag atgataaaga tgatcttcag agagcaattg ccttgagttt ggccgaatca 1981 aacagggcat tcagggagac tggaataact gatgaggaac aagccattag cagagttctt 2041 gaagccagca tagcagagaa taaagcatgt ttgaagagga cacctacaga agtttggagg 2101 gattctcgaa acccttatga tagaaaaaga caggacaaag ctcccgttgg gctaaagaat 2161 gttggcaata cttgttggtt tagtgctgtt attcagtcat tatttaatct tttggaattt 2221 agaagattag ttctgaatta caagcctcca tcaaatgctc aagatttacc ccgaaaccaa 2281 aaggaacatc ggaatttgcc ttttatgcgt gagctgaggt atctatttgc acttcttgtt 2341 ggtaccaaaa ggaagtatgt tgatccatca agagcagttg aaattcttaa ggatgctttc 2401 aaatcaaatg actcacagca gcaagatgtg agtgagttta cacacaaatt attagattgg 2461 ttagaagatg ccttccaaat gaaagctgaa gaggagacgg atgaagagaa gccaaagaac 2521 cccatggtag agttgttcta tggcagattc ctggctgtgg gagtacttga aggtaaaaaa 2581 tttgaaaaca ctgaaatgtt tggtcagtac ccacttcagg tcaatgggtt caaagatctg 2641 catgagtgcc tagaagctgc aatgattgaa ggagaaattg agtctttaca ttcagagaat 2701 tcaggaaaat caggccaaga gcattggttt actgaattac cacctgtgtt aacatttgaa 2761 ttgtcaagat ttgaatttaa tcaggcattg ggaagaccag aaaaaattca caacaaatta 2821 gaatttcccc aagttttata tttggacaga tacatgcaca gaaacagaga aataacaaga 2881 attaagaggg aagagatcaa gagactgaaa gattacctca cggtattaca acaaaggcta 2941 gaaagatatt taagctatgg ttccggtccc aaacgattcc ccttggtaga tgttcttcag 3001 tatgcattgg aatttgcctc aagtaaacct gtttgcactt ctcctgttga cgatattgac 3061 gctagttccc cacctagtgg ttccatacca tcacagacat taccaagcac aacagaacaa 3121 cagggagccc tatcttcaga actgccaagc acatcacctt catcagttgc tgccatttca 3181 tcgagatcag taatacacaa accatttact cagtcccgga tacctccaga tttgcccatg 3241 catccggcac caaggcacat aacggaggaa gaactttctg tgctggaaag ttgtttacat 3301 cgctggagga cagaaataga aaatgacacc agagatttgc aggaaagcat atccagaatc 3361 catcgaacaa ttgaattaat gtactctgac aaatctatga tacaagttcc ttatcgatta 3421 catgccgttt tagttcacga aggccaagct aatgctgggc actactgggc atatattttt 3481 gatcatcgtg aaagcagatg gatgaagtac aatgatattg ctgtgacaaa atcatcatgg 3541 gaagagctag tgagggactc ttttggtggt tatagaaatg ccagtgcata ctgtttaatg 3601 tacataaatg ataaggcaca gttcctaata caagaggagt ttaataaaga aactgggcag 3661 ccccttgttg gtatagaaac attaccaccg gatttgagag attttgttga ggaagacaac 3721 caacgatttg aaaaagaact agaagaatgg gatgcacaac ttgcccagaa agctttgcag 3781 gaaaagcttt tagcgtctca gaaattgaga gagtcagaga cttctgtgac aacagcacaa 3841 gcagcaggag acccagaata tctagagcag ccatcaagaa gtgatttctc aaagcacttg 3901 aaagaagaaa ctattcaaat aattaccaag gcatcacatg agcatgaaga taaaagtcct 3961 gaaacagttt tgcagtcggc aattaagttg gaatatgcaa ggttggttaa gttggcccaa 4021 gaagacaccc caccagaaac cgattatcgt ttacatcatg tagtggtcta ctttatccag 4081 aaccaggcac caaagaaaat tattgagaaa acattactag aacaatttgg agatagaaat 4141 ttgagttttg atgaaaggtg tcacaacata atgaaagttg ctcaagccaa actggaaatg 4201 ataaaacctg aagaagtaaa cttggaggaa tatgaggagt ggcatcagga ttataggaaa 4261 ttcagggaaa caactatgta tctcataatt gggctagaaa attttcaaag agaaagttat 4321 atagattcct tgctgttcct catctgtgct tatcagaata acaaagaact cttgtctaaa 4381 ggcttataca gaggacatga tgaagaattg atatcacatt atagaagaga atgtttgcta 4441 aaattaaatg agcaagccgc agaactcttc gaatctggag aggatcgaga agtaaacaat 4501 ggtttgatta tcatgaatga gtttattgtc ccatttttgc cattattact ggtggatgaa 4561 atggaagaaa aggatatact agctgtagaa gatatgagaa atcgatggtg ttcctacctt 4621 ggtcaagaaa tggaaccaca cctccaagaa aagctgacag attttttgcc aaaactgctt 4681 gattgttcta tggagattaa aagtttccat gagccaccga agttaccttc atattccacg 4741 catgaactct gtgagcgatt tgcccgaatc atgttgtccc tcagtcgaac tcctgctgat 4801 ggaagataaG GATCCtGATA TCCAGCACAG TGGCGGCCGC TCGAGTCTAG AGGGCCCGTT 4861 TAAACCCGCT GATCAGCCTC GACTGTGCCT TCTAGTTGCC AGCCATCTGT TGTTTGCCCC 4921 TCCCCCGTGC CTTCCTTGAC CCTGGAAGGT GCCACTCCCA CTGTCCTTTC CTAATAAAAT 4981 GAGGAAATTG CATCGCATTG TCTGAGTAGG TGTCATTCTA TTCTGGGGGG TGGGGTGGGG 5041 CAGGACAGCA AGGGGGAGGA TTGGGAAGAC AATAGCAGGC ATGCTGGGGA TGCGGTGGGC 5101 TCTATGGCTT CTGAGGCGGA AAGAACCAGC TGGGGCTCTA GGGGGTATCC CCACGCGCCC 5161 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 5221 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 5281 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 5341 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 5401 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 5461 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 5521 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 5581 TAATTCTGTG GAATGTGTGT CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA 5641 GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT 5701 CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC ATAGTCCCGC 5761 CCCTAACTCC GCCCATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG 5821 GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCTGCCTCT GAGCTATTCC 5881 AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT 5941 GTATATCCAT TTTCGGATCT GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC 6001 AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT 6061 GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC 6121 GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG 6181 CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG 6241 TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT 6301 CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC 6361 ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG 6421 CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG 6481 GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGAGGATC 6541 TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT 6601 CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG CTATCAGGAC ATAGCGTTGG 6661 CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT 6721 ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT 6781 TCTGAGCGGG ACTCTGGGGT TCGAAATGAC CGACCAAGCG ACGCCCAACC TGCCATCACG 6841 AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC TTCGGAATCG TTTTCCGGGA 6901 CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG GAGTTCTTCG CCCACCCCAA 6961 CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA 7021 TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA 7081 TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG TAATCATGGT CATAGCTGTT 7141 TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA 7201 GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT 7261 GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC 7321 GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG 7381 CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 7441 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 7501 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 7561 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 7621 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 7681 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 7741 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 7801 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 7861 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 7921 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 7981 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 8041 CGGCAAACAA ACCACCGCTG GTAGCGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 8101 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 8161 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 8221 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC 8281 TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC 8341 ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC 8401 TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC 8461 AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC 8521 CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT 8581 GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC 8641 TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA 8701 AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT 8761 ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG 8821 CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC 8881 GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA 8941 AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT 9001 GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT 9061 CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG 9121 GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA 9181 TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT 9241 AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCGACGGAT CGGGAGATCT 9301 CCCGATCCCC TATGGT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.