pLV3-CMV-DCAF5-3×FLAG-Puro pla

pLV3-CMV-DCAF5-3×FLAG-Puro plasmid

PVT96809

pLV3-CMV-DCAF5-3×FLAG-Puro plasmid Information

Plasmid name: pLV3-CMV-DCAF5(human)-3×FLAG-Puro
Alias: NM_003861.3;BCRG2; BCRP2; D14S1461E; WDR22
Gene lenght: 2826bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-DCAF5-3×FLAG-Puro plasmid Description

pLV3-CMV-DCAF5-3×FLAG-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-DCAF5-3×FLAG-Puro plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT 1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT 1921 ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA 1981 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC 2041 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA 2101 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT 2161 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG 2221 TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGATTCT 2281 AGAGCTAGCG AATTCgccac catgaagagg agagctggcc tggggggcag catgaggtca 2341 gtggtgggct tcttgtccca gcggggcttg catggggacc ccctgctcac tcaggacttt 2401 cagaggagac gcctgcgggg ctgcagaaac ctctacaaga aggacctcct cggccacttc 2461 ggctgtgtca atgccattga attctccaac aatggaggcc agtggctggt ctcaggagga 2521 gatgaccgcc gggttctgct atggcacatg gaacaagcca tccactccag ggtcaagccc 2581 atacagctga aaggagagca ccattccaac attttttgcc tggctttcaa cagtgggaac 2641 actaaagtgt tctctggagg caatgatgag caagttatcc tccatgatgt tgaaagcagt 2701 gagacattgg acgtgtttgc tcatgaagat gcagtatatg gcttgtctgt gagcccagtg 2761 aatgacaaca tttttgccag ttcctcagat gatggccggg ttctcatttg ggacattcgg 2821 gaatcccccc atggagagcc cttctgcctg gcaaactatc catcagcctt tcatagtgtc 2881 atgtttaacc ctgtggagcc caggttgttg gccacagcca attcaaagga aggagtggga 2941 ctctgggaca ttcgaaaacc tcagagttct ctcctgcgct atggtggaaa cctgtccctc 3001 caaagtgcca tgagtgtacg attcaacagc aacgggaccc agctcctggc cctgaggcga 3061 cgcctgcccc ctgtgctcta tgacatccat tcccgcctgc ctgtgtttca gtttgacaat 3121 cagggttact tcaactcatg caccatgaaa agctgctgtt ttgcaggaga tcgtgaccag 3181 tatatccttt cgggctctga tgacttcaac ctgtacatgt ggagaattcc tgcagatcca 3241 gaagcaggtg gcattggtag ggtggtcaac ggagccttca tggtgctgaa agggcatcga 3301 tctattgtta accaagtccg atttaatccc cacacctaca tgatctgctc ttctggtgta 3361 gaaaagatta tcaagatctg gagcccatac aagcagccag gatgtactgg agacctcgac 3421 ggtcggattg aggacgattc ccgctgcctc tatacccatg aagagtacat cagccttgtg 3481 ctgaacagtg ggagtggcct gtcgcatgac tacgccaacc agtcggtcca ggaagacccc 3541 cggatgatgg ccttctttga ctcactggta cgccgagaga tcgagggctg gagctctgac 3601 tcagacagtg acctcagtga gagtactatc ctccaactgc acgctggggt cagcgagcgc 3661 tcaggctaca ctgactcaga gtcttcggcc tcattgcctc gctccccgcc tcccacagta 3721 gatgagtctg ccgacaacgc cttccacctg gggcccctgc gggtcaccac cacaaacaca 3781 gtagcctcaa ctccaccaac acccacgtgt gaggatgcag cctctcgcca gcagcgtctg 3841 tctgctctgc ggcgctacca agacaaacgc ctcctggccc tttccaatga gtccgattct 3901 gaggagaatg tctgtgaggt ggaactagac acagatctct ttccccggcc acggtcaccc 3961 agccccgaag atgaatccag cagttccagc agctctagca gctctgagga tgaggaggag 4021 ctgaatgaac gccgagcctc tacctggcag cggaatgcca tgcggcgccg acagaagaca 4081 acccgagaag acaagcccag tgccccaatc aagcccacca acacttacat tggagaagac 4141 aactatgatt acccccagat caaagtggat gacctctcct cctccccaac ctcgtcccct 4201 gagcggagca cttccacgct agagattcaa ccaagccggg catcaccaac ttctgacata 4261 gaatcagttg agcgaaaaat ttataaagct tacaagtggc tccgctactc ttatatctcc 4321 tactcaaata acaaagatgg agagacctcc ttggtgaccg gggaggcaga tgaagggaga 4381 gcaggaacca gccacaaaga caacccagcc ccttcttcca gtaaggaagc ctgtctaaac 4441 atagcaatgg cccagaggaa ccaggacctg ccacctgaag gctgcagcaa ggacactttt 4501 aaagaagaga ctcctagaac tcccagcaat ggcccaggcc atgagcacag cagccatgct 4561 tgggcagagg tgccagaggg tacctctcag gacactggca atagcggctc tgtagagcac 4621 ccttttgaaa ccaagaagct caatggaaag gccctgagca gtcgggctga ggagccgcct 4681 tctcctcctg tccccaaggc atctggctcc actctcaaca gcgggtctgg caactgtccc 4741 aggacccagt ctgatgacag tgaggagagg agcctcgaaa ccatctgtgc caaccacaac 4801 aatggacgct tacaccctcg tccccctcac cctcacaata acgggcagaa cttgggggag 4861 ctggaggtgg tggcctactc ttccccagga cactcagaca ctgaccgtga taactcgtcc 4921 ctgacaggga cactcctaca caaagattgt tgcgggtctg aaatggcctg tgagaccccc 4981 aatgctggaa caagagagga ccccactgac accccagcca cagatagtag cagggctgtt 5041 catggccaca gtggcctcaa aaggcaacga attgaattgg aagatacaga ttcagagaat 5101 tcctcctcag agaagaaatt aaaaacaGGA TCCGACTACA AAGACCATGA CGGTGATTAT 5161 AAAGATCATG ACATCGACTA CAAGGATGAC GATGACAAGT AGCGGCCGCG AAGGATCTGC 5221 GATCGCTCCG GTGCCCGTCA GTGGGCAGAG CGCACATCGC CCACAGTCCC CGAGAAGTTG 5281 GGGGGAGGGG TCGGCAATTG AACGGGTGCC TAGAGAAGGT GGCGCGGGGT AAACTGGGAA 5341 AGTGATGTCG TGTACTGGCT CCGCCTTTTT CCCGAGGGTG GGGGAGAACC GTATATAAGT 5401 GCAGTAGTCG CCGTGAACGT TCTTTTTCGC AACGGGTTTG CCGCCAGAAC ACAGCTGAAG 5461 CTTCGAGGGG CTCGCATCTC TCCTTCACGC GCCCGCCGCC CTACCTGAGG CCGCCATCCA 5521 CGCCGGTTGA GTCGCGTTCT GCCGCCTCCC GCCTGTGGTG CCTCCTGAAC TGCGTCCGCC 5581 GTCTAGGTAA GTTTAAAGCT CAGGTCGAGA CCGGGCCTTT GTCCGGCGCT CCCTTGGAGC 5641 CTACCTAGAC TCAGCCGGCT CTCCACGCTT TGCCTGACCC TGCTTGCTCA ACTCTACGTC 5701 TTTGTTTCGT TTTCTGTTCT GCGCCGTTAC AGATCCAAGC TGTGACCGGC GCCTACGCTA 5761 GATGACCGAG TACAAGCCCA CGGTGCGCCT CGCCACCCGC GACGACGTCC CCCGGGCCGT 5821 ACGCACCCTC GCCGCCGCGT TCGCCGACTA CCCCGCCACG CGCCACACCG TCGACCCGGA 5881 CCGCCACATC GAGCGGGTCA CCGAGCTGCA AGAACTCTTC CTCACGCGCG TCGGGCTCGA 5941 CATCGGCAAG GTGTGGGTCG CGGACGACGG CGCCGCGGTG GCGGTCTGGA CCACGCCGGA 6001 GAGCGTCGAA GCGGGGGCGG TGTTCGCCGA GATCGGCCCG CGCATGGCCG AGTTGAGCGG 6061 TTCCCGGCTG GCCGCGCAGC AACAGATGGA AGGCCTCCTG GCGCCGCACC GGCCCAAGGA 6121 GCCCGCGTGG TTCCTGGCCA CCGTCGGCGT CTCGCCCGAC CACCAGGGCA AGGGTCTGGG 6181 CAGCGCCGTC GTGCTCCCCG GAGTGGAGGC GGCCGAGCGC GCCGGGGTGC CCGCCTTCCT 6241 GGAGACCTCC GCGCCCCGCA ACCTCCCCTT CTACGAGCGG CTCGGCTTCA CCGTCACCGC 6301 CGACGTCGAG GTGCCCGAAG GACCGCGCAC CTGGTGCATG ACCCGCAAGC CCGGTGCCTG 6361 AGTCGACAAT CAACCTCTGG ATTACAAAAT TTGTGAAAGA TTGACTGGTA TTCTTAACTA 6421 TGTTGCTCCT TTTACGCTAT GTGGATACGC TGCTTTAATG CCTTTGTATC ATGCTATTGC 6481 TTCCCGTATG GCTTTCATTT TCTCCTCCTT GTATAAATCC TGGTTGCTGT CTCTTTATGA 6541 GGAGTTGTGG CCCGTTGTCA GGCAACGTGG CGTGGTGTGC ACTGTGTTTG CTGACGCAAC 6601 CCCCACTGGT TGGGGCATTG CCACCACCTG TCAGCTCCTT TCCGGGACTT TCGCTTTCCC 6661 CCTCCCTATT GCCACGGCGG AACTCATCGC CGCCTGCCTT GCCCGCTGCT GGACAGGGGC 6721 TCGGCTGTTG GGCACTGACA ATTCCGTGGT GTTGTCGGGG AAATCATCGT CCTTTCCTTG 6781 GCTGCTCGCC TGTGTTGCCA CCTGGATTCT GCGCGGGACG TCCTTCTGCT ACGTCCCTTC 6841 GGCCCTCAAT CCAGCGGACC TTCCTTCCCG CGGCCTGCTG CCGGCTCTGC GGCCTCTTCC 6901 GCGTCTTCGC CTTCGCCCTC AGACGAGTCG GATCTCCCTT TGGGCCGCCT CCCCGCCTGG 6961 TACCTTTAAG ACCAATGACT TACAAGGCAG CTGTAGATCT TAGCCACTTT TTAAAAGAAA 7021 AGGGGGGACT GGAAGGGCTA ATTCACTCCC AACGAAAATA AGATCTGCTT TTTGCTTGTA 7081 CTGGGTCTCT CTGGTTAGAC CAGATCTGAG CCTGGGAGCT CTCTGGCTAA CTAGGGAACC 7141 CACTGCTTAA GCCTCAATAA AGCTTGCCTT GAGTGCTTCA AGTAGTGTGT GCCCGTCTGT 7201 TGTGTGACTC TGGTAACTAG AGATCCCTCA GACCCTTTTA GTCAGTGTGG AAAATCTCTA 7261 GCAGTAGTAG TTCATGTCAT CTTATTATTC AGTATTTATA ACTTGCAAAG AAATGAATAT 7321 CAGAGAGTGA GAGGAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA 7381 TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC 7441 TCATCAATGT ATCTTATCAT GTCTGGCTCT AGCTATCCCG CCCCTAACTC CGCCCAGTTC 7501 CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC 7561 CTCGGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGACTTTTG 7621 CAGAGACGGC CCAAATTCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC 7681 GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA 7741 ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA 7801 CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT 7861 TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG 7921 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 7981 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 8041 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 8101 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 8161 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 8221 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 8281 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 8341 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 8401 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 8461 GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA 8521 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 8581 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 8641 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 8701 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 8761 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT 8821 AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT 8881 CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT 8941 GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG 9001 AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG 9061 TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT 9121 TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC 9181 CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT 9241 CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC 9301 AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA 9361 GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC 9421 GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA 9481 ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA 9541 ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG 9601 AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG 9661 AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT 9721 GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT 9781 TCCCCGAAAA GTGCCACCTG ACGTCTAAGA AACCATTATT ATCATGACAT TAACCTATAA 9841 AAATAGGCGT ATCACGAGGC CCTTTCGTCT CGCGCGTTTC GGTGATGACG GTGAAAACCT 9901 CTGACACATG CAGCTCCCGG AGACGGTCAC AGCTTGTCTG TAAGCGGATG CCGGGAGCAG 9961 ACAAGCCCGT CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT CGGGGCTGGC TTAACTATGC 10021 GGCATCAGAG CAGATTGTAC TGAGAGTGCA CCATATGCGG TGTGAAATAC CGCACAGATG 10081 CGTAAGGAGA AAATACCGCA TCAGGCGCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA 10141 AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC 10201 AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC 10261 CAGTGCCAAG CTG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.