pZHX2-Fluc plasmid

pZHX2-Fluc plasmid

PVT96791

pZHX2-Fluc plasmid Information

Plasmid name: pZHX2(human)-Fluc
Alias: -
Gene lenght: 2200bp
Host: Mammalian cells
Use(s):
Promoter detection
Fragment type: Promoter
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Fluc
Promoter: ZHX2
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: Fluc
Reverse primer:
Copy number: High
Induction:

pZHX2-Fluc plasmid Description

pZHX2-Fluc is a plasmid suitable for promoter detection in mammalian cells.

pZHX2-Fluc plasmid Sequence

1 ggtaccTACA ATTTTGGTTC ACCCTGGGTG AGGCAGAGGT TGGGAGGAGA TCAGGCAGCG 61 GTAAATTCTG ATGTTGATGG CAAGTAAATT AGGTTCTTAG CCGGTACCAA ATGAGGCTGA 121 GAAAGATGTG GCCCAAGCTG GATTACAGTC ATCAAGAAGT TAACAGAATC CTGTAAGAGC 181 CCCTCCAGGT CGCCTTTCAG GTCCGCGAGC ATCACGCAAG AGACTCCTTC CCAGGATAGA 241 ATCTTGTTTT ATGGGCGGTT AAAATGCAAA CAAACAGACC CTACATCAGC AATAACCGAA 301 CAAACAGGTG CAGGCCATTA AGAAAGTTTT GCATATGAGC TGTTTTAATT TTTGTTGCAA 361 AGGATTTCTG GGGAGTCAAG GGGTATGTCA GAAATTAGAG CCCTAGGGCA GAACTTACAA 421 AAACCTTTTA GCTACTTCCT TCTCCCCGGC CTCGGAAATG AAAAAGCCAA AAGGTCACCC 481 AGCGCATCCG CCACCCAGTC AGCCCCAAGG CCAGCCGTCC TCACCCCAGT CCGGGTCCTC 541 GCCATTGGCC TCTCCGCTGC ACACGGCCAC CTGTTGATGC CCTCGCGCCC ACAGCCGAGT 601 TGCAAACGGC CGCTAGAGTT CGTTCTAATT CCCCAGAGCA GAGCTGCCCG GGAAGCCCCG 661 ACGCGACGCG CCTGCTAATC TGCAGGGCGG GCGGGGACTC CGCAGAGCCT CCCAGCCCCA 721 GGCTCCCTTT GTTTTCTCGC TGCGCTCGAA GCTTCTAAAC TAGCCCCCAG ACCGCATCCC 781 CTCGTCCCCG CCCGGGAGCT GCCCAAGAGT CCACAGGCCA GCCCAGGGGG AAACAAGGTA 841 CATCTGGGCA GGAATGCGCG GCGCAGACAG GTTGCCGCCT GAGATCCCGA AGTGTACCCA 901 GATCCCGAAT TCCTTAGAGC TCTTTAGCCG AGCACTGTGG CAGGAGCGCG GCGCCCGGAA 961 TACAAGTTCA TGGTTCCGAG CCGGCGGGCG AAGGGCAGCG CGATCCCCCG CCACCTTTGC 1021 TCTAAGGCGC CTAGGGGAAC CTGGGGGCTG CCGCGGTGAG AGCTCCGCTC CCACTGGGAA 1081 CATTGGAGTC CCAGTTGCGA AAGCGACCCG CCTAGCTTCC GAGGGACTTC TCTGCGAGCA 1141 CCGCAGGCGT GTGCATTTCT TCGAATCTGA GAATTCCAGC GAACTCGGGC TCTACCAGAG 1201 TTTAAGTCGG CCCCTGGGGC GTCTGTTTGT TTATTGGCTC TTAAGGGAAA AAAGTTTGAA 1261 GATCTTTAAC CAGCCTCAGA CCTGGCGCGG GCGGAAATCC CGGAATCTCC CCAAGCAAAA 1321 GTGATATAAC TTCAAGTCGT TATATAAGTG GGGGGCTCCT TCTTCCCCTC CCTACGCCGA 1381 GCCTTCTCAT TATCATTCCG GGAGTAAAAT GTGTCTCGCT CAACATAGTT TTAAAATCGT 1441 TATTATTATT TTCCTGAGCC TGAAGCCGGG CCAAGGCGCA GGCAGCGCCC AGGGTGCGTG 1501 GTGGCCCCAC CCGCGCACAG TCCCACGCAA AGGGTCCCGG GGCCCGGGTA CCGCCAGAGC 1561 CGGGCCTGCA AGCGCGCCCT TCGGTCCCAC GGTCCGCCAC TCCACGTGGT CAAGATCAAC 1621 AGTTTGTTGA GCTCATCAGT GACACCTTGA ACTTTTCATA AAGAAAATGG GTATTGCACG 1681 GAGACGGTTT GGCCGCGGCC GCCGCGCGGG GTCGTGCTTA ATATTCAATG ATCTCGCTCG 1741 CGCCTCCCCC TCCTCTTCTC GCAGAAATTC CACCGAGTCC GTTACTGGGC TCAGACTTTT 1801 AAGTTAATTT ACTGCCCCCC TACCTTGGCC AGTAACCAGC GCCTTTAGGG CTAGCCTCCC 1861 CCCCAGCTCC TGCCTGAAAA ATGACATTTC GCCGGTGTCT CCGGAGGGGG CTGAATTTCA 1921 CTTTGTAACT TTCTGCGGAA CCCGAGCCCG GGTGGCAGCT CGGGTGGTGG TATCGTATGC 1981 AAATACGCAT GCTGACGTTA CAGATCATGT GGGTTTTGGC GTAGATTCCC CACTGATCGA 2041 GGCATTTTTT TTCCCTTTTT TTTTCCTTTT TTTTTTTCTT TTAAAAATTT TGGCCATCGT 2101 TCTCCGTACG GGGGCTTTTT CTGTCTGTCT GTCTGGCTGG CAGGCTGGCT TTCCCCCTCT 2161 TTCCCACGGA GCCCGAGCCG GGCGCCCGGT GGGGAGTGGG GAGTGGaagc ttggcattcc 2221 ggtactgttg gtaaagccac catggaagac gccaaaaaca taaagaaagg cccggcgcca 2281 ttctatccgc tggaagatgg aaccgctgga gagcaactgc ataaggctat gaagagatac 2341 gccctggttc ctggaacaat tgcttttaca gatgcacata tcgaggtgga catcacttac 2401 gctgagtact tcgaaatgtc cgttcggttg gcagaagcta tgaaacgata tgggctgaat 2461 acaaatcaca gaatcgtcgt atgcagtgaa aactctcttc aattctttat gccggtgttg 2521 ggcgcgttat ttatcggagt tgcagttgcg cccgcgaacg acatttataa tgaacgtgaa 2581 ttgctcaaca gtatgggcat ttcgcagcct accgtggtgt tcgtttccaa aaaggggttg 2641 caaaaaattt tgaacgtgca aaaaaagctc ccaatcatcc aaaaaattat tatcatggat 2701 tctaaaacgg attaccaggg atttcagtcg atgtacacgt tcgtcacatc tcatctacct 2761 cccggtttta atgaatacga ttttgtgcca gagtccttcg atagggacaa gacaattgca 2821 ctgatcatga actcctctgg atctactggt ctgcctaaag gtgtcgctct gcctcataga 2881 actgcctgcg tgagattctc gcatgccaga gatcctattt ttggcaatca aatcattccg 2941 gatactgcga ttttaagtgt tgttccattc catcacggtt ttggaatgtt tactacactc 3001 ggatatttga tatgtggatt tcgagtcgtc ttaatgtata gatttgaaga agagctgttt 3061 ctgaggagcc ttcaggatta caagattcaa agtgcgctgc tggtgccaac cctattctcc 3121 ttcttcgcca aaagcactct gattgacaaa tacgatttat ctaatttaca cgaaattgct 3181 tctggtggcg ctcccctctc taaggaagtc ggggaagcgg ttgccaagag gttccatctg 3241 ccaggtatca ggcaaggata tgggctcact gagactacat cagctattct gattacaccc 3301 gagggggatg ataaaccggg cgcggtcggt aaagttgttc cattttttga agcgaaggtt 3361 gtggatctgg ataccgggaa aacgctgggc gttaatcaaa gaggcgaact gtgtgtgaga 3421 ggtcctatga ttatgtccgg ttatgtaaac aatccggaag cgaccaacgc cttgattgac 3481 aaggatggat ggctacattc tggagacata gcttactggg acgaagacga acacttcttc 3541 atcgttgacc gcctgaagtc tctgattaag tacaaaggct atcaggtggc tcccgctgaa 3601 ttggaatcca tcttgctcca acaccccaac atcttcgacg caggtgtcgc aggtcttccc 3661 gacgatgacg ccggtgaact tcccgccgcc gttgttgttt tggagcacgg aaagacgatg 3721 acggaaaaag agatcgtgga ttacgtcgcc agtcaagtaa caaccgcgaa aaagttgcgc 3781 ggaggagttg tgtttgtgga cgaagtaccg aaaggtctta ccggaaaact cgacgcaaga 3841 aaaatcagag agatcctcat aaaggccaag aagggcggaa agatcgccgt gtaattctag 3901 agtcggggcg gccggccgct tcgagcagac atgataagat acattgatga gtttggacaa 3961 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct 4021 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt 4081 atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa 4141 tgtggtaaaa tcgataagga tccgtcgacc gatgcccttg agagccttca acccagtcag 4201 ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg tcttctttat 4261 catgcaactc gtaggacagg tgccggcagc gctcttccgc ttcctcgctc actgactcgc 4321 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 4381 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 4441 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 4501 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 4561 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 4621 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct 4681 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 4741 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 4801 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 4861 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag 4921 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 4981 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 5041 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 5101 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 5161 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 5221 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat 5281 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct 5341 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt 5401 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat 5461 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 5521 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg 5581 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 5641 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 5701 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 5761 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 5821 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa 5881 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 5941 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 6001 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 6061 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa 6121 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 6181 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg 6241 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg 6301 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc 6361 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc 6421 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga 6481 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa 6541 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga 6601 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca 6661 aaatattaac gtttacaatt tcccattcgc cattcaggct gcgcaactgt tgggaagggc 6721 gatcggtgcg ggcctcttcg ctattacgcc agcccaagct accatgataa gtaagtaata 6781 ttaaggtacg ggaggtactt ggagcggccg caataaaata tctttatttt cattacatct 6841 gtgtgttggt tttttgtgtg aatcgatagt actaacatac gctctccatc aaaacaaaac 6901 gaaacaaaac aaactagcaa aataggctgt ccccagtgca agtgcaggtg ccagaacatt 6961 tctctatcga ta

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.