pLV3-CMV-SDHA-3×FLAG-CopGFP-Pu

pLV3-CMV-SDHA-3×FLAG-CopGFP-Puro plasmid

PVT95467

pLV3-CMV-SDHA-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-SDHA(human)-3×FLAG-CopGFP-Puro
Alias: NM_004168.4;CMD1GG; FP; MC2DN1; NDAXOA; PGL5; PPGL5;SDH1; SDH2; SDHF
Gene lenght: 1992bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-SDHA-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-SDHA-3×FLAG-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-SDHA-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgt cgggggtccg gggcctgtcg 2341 cggctgctga gcgctcggcg cctggcgctg gccaaggcgt ggccaacagt gttgcaaaca 2401 ggaacccgag gttttcactt cactgttgat gggaacaaga gggcatctgc taaagtttca 2461 gattccattt ctgctcagta tccagtagtg gatcatgaat ttgatgcagt ggtggtaggc 2521 gctggagggg caggcttgcg agctgcattt ggcctttctg aggcagggtt taatacagca 2581 tgtgttacca agctgtttcc taccaggtca cacactgttg cagcacaggg aggaatcaat 2641 gctgctctgg ggaacatgga ggaggacaac tggaggtggc atttctacga caccgtgaag 2701 ggctccgact ggctggggga ccaggatgcc atccactaca tgacggagca ggcccccgcc 2761 gccgtggtcg agctagaaaa ttatggcatg ccgtttagca gaactgaaga tgggaagatt 2821 tatcagcgtg catttggtgg acagagcctc aagtttggaa agggcgggca ggcccatcgg 2881 tgctgctgtg tggctgatcg gactggccac tcgctattgc acaccttata tggaaggtct 2941 ctgcgatatg ataccagcta ttttgtggag tattttgcct tggatctcct gatggagaat 3001 ggggagtgcc gtggtgtcat cgcactgtgc atagaggacg ggtccatcca tcgcataaga 3061 gcaaagaaca ctgttgttgc cacaggaggc tacgggcgca cctacttcag ctgcacgtct 3121 gcccacacca gcactggcga cggcacggcc atgatcacca gggcaggcct tccttgccag 3181 gacctagagt ttgttcagtt ccaccctaca ggcatatatg gtgctggttg tctcattacg 3241 gaaggatgtc gtggagaggg aggcattctc attaacagtc aaggcgaaag gtttatggag 3301 cgatacgccc ctgtcgcgaa ggacctggcg tctagagatg tggtgtctcg gtccatgact 3361 ctggagatcc gagaaggaag aggctgtggc cctgagaaag atcacgtcta cctgcagctg 3421 caccacctac ctccagagca gctggccacg cgcctgcctg gcatttcaga gacagccatg 3481 atcttcgctg gcgtggacgt cacgaaggag ccgatccctg tcctccccac cgtgcattat 3541 aacatgggcg gcattcccac caactacaag gggcaggtcc tgaggcacgt gaatggccag 3601 gatcagattg tgcccggcct gtacgcctgt ggggaggccg cctgtgcctc ggtacatggt 3661 gccaaccgcc tcggggcaaa ctcgctcttg gacctggttg tctttggtcg ggcatgtgcc 3721 ctgagcatcg aagagtcatg caggcctgga gataaagtcc ctccaattaa accaaacgct 3781 ggggaagaat ctgtcatgaa tcttgacaaa ttgagatttg ctgatggaag cataagaaca 3841 tcggaactgc gactcagcat gcagaagtca atgcaaaatc atgctgccgt gttccgtgtg 3901 ggaagcgtgt tgcaagaagg ttgtgggaaa atcagcaagc tctatggaga cctaaagcac 3961 ctgaagacgt tcgaccgggg aatggtctgg aacacggacc tggtggagac cctggagctg 4021 cagaacctga tgctgtgtgc gctgcagacc atctacggag cagaggcacg gaaggagtca 4081 cggggcgcgc atgccaggga agactacaag gtgcggattg atgagtacga ttactccaag 4141 cccatccagg ggcaacagaa gaagcccttt gaggagcact ggaggaagca caccctgtcc 4201 tatgtggacg ttggcactgg gaaggtcact ctggaatata gacccgtgat cgacaaaact 4261 ttgaacgagg ctgactgtgc caccgtcccg ccagccattc gctcctacgg atccGACTAC 4321 AAAGACCATG ACGGTGATTA TAAAGATCAT GACATCGACT ACAAGGATGA CGATGACAAG 4381 tagcggccgc Gaaggatctg cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg 4441 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg 4501 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt 4561 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt 4621 gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc 4681 cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt 4741 gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag accgggcctt 4801 tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc 4861 ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta cagatccaag 4921 ctgtgaccgg cgcctacgct agacgccacc atggagagcg acgagagcgg cctgcccgcc 4981 atggagatcg agtgccgcat caccggcacc ctgaacggcg tggagttcga gctggtgggc 5041 ggcggagagg gcacccccaa gcagggccgc atgaccaaca agatgaagag caccaaaggc 5101 gccctgacct tcagccccta cctgctgagc cacgtgatgg gctacggctt ctaccacttc 5161 ggcacctacc ccagcggcta cgagaacccc ttcctgcacg ccatcaacaa cggcggctac 5221 accaacaccc gcatcgagaa gtacgaggac ggcggcgtgc tgcacgtgag cttcagctac 5281 cgctacgagg ccggccgcgt gatcggcgac ttcaaggtgg tgggcaccgg cttccccgag 5341 gacagcgtga tcttcaccga caagatcatc cgcagcaacg ccaccgtgga gcacctgcac 5401 cccatgggcg ataacgtgct ggtgggcagc ttcgcccgca ccttcagcct gcgcgacggc 5461 ggctactaca gcttcgtggt ggacagccac atgcacttca agagcgccat ccaccccagc 5521 atcctgcaga acgggggccc catgttcgcc ttccgccgcg tggaggagct gcacagcaac 5581 accgagctgg gcatcgtgga gtaccagcac gccttcaaga cccccatcgc cttcgccaga 5641 tcccgcgctc agtcgtccaa ttctgccgtg gacggcaccg ccggacccgg ctccaccgga 5701 tctcgcgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccggccct 5761 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta 5821 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac 5881 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac 5941 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag 6001 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt 6061 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag 6121 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc 6181 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg 6241 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc 6301 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga 6361 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct 6421 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt 6481 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg 6541 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact 6601 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct 6661 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg 6721 ttgggcactg acaattccgt ggtgttgtcg gggaaATCAT CGTCCTTTCC Ttggctgctc 6781 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc 6841 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctT 6901 cgccttcgcc ctcagacgag tcggatctcc ctttggGccg cctccccgcc tggtaccttt 6961 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg 7021 actggaaggg ctaattcact cccaacgaaG ataagatctg ctttttgctt gtactgggtc 7081 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 7141 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 7201 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag 7261 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag 7321 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 7381 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 7441 tgtatcttat catgtctggc tctagctaTC CCGCCCCTAA CTCCGCCCAt cccgccccta 7501 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca 7561 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga 7621 ggcctagact tttgcagaga ccaaattcgt aatcatgtca tagctgtttc ctgtgtgaaa 7681 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg 7741 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca 7801 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg 7861 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 7921 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 7981 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 8041 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 8101 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 8161 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 8221 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 8281 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 8341 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 8401 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 8461 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc 8521 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 8581 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 8641 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 8701 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 8761 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 8821 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 8881 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 8941 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 9001 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 9061 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 9121 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 9181 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 9241 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 9301 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 9361 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 9421 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 9481 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 9541 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 9601 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 9661 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 9721 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 9781 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt 9841 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg 9901 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc 9961 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct 10021 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc 10081 gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa 10141 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg 10201 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa 10261 aacgacggcc agtgccaagc tg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.