pCMV-MYH9-3×FLAG-mCMV-EGFP pla

pCMV-MYH9-3×FLAG-mCMV-EGFP plasmid

PVT96777

pCMV-MYH9-3×FLAG-mCMV-EGFP plasmid Information

Plasmid name: pCMV-MYH9(human)-3×FLAG-mCMV-EGFP
Alias: NM_002473.6;BDPLT6; DFNA17; EPSTS; FTNS; MATINS; MHA;NMHC-II-A; NMMHC-IIA; NMMHCA
Gene lenght: 5880bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker:
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-MYH9-3×FLAG-mCMV-EGFP plasmid Description

pCMV-MYH9-3×FLAG-mCMV-EGFP is a plasmid suitable for protein expression in mammalian cells.

pCMV-MYH9-3×FLAG-mCMV-EGFP plasmid Sequence

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc GCTAGCGCTA 601 CCGGACTCAG ATCTCGAGCT CCGAATTCgc caccatggca cagcaagctg ccgataagta 661 tctctatgtg gataaaaact tcatcaacaa tccgctggcc caggccgact gggctgccaa 721 gaagctggta tgggtgcctt ccgacaagag tggctttgag ccagccagcc tcaaggagga 781 ggtgggcgaa gaggccatcg tggagctggt ggagaatggg aagaaggtga aggtgaacaa 841 ggatgacatc cagaagatga acccgcccaa gttctccaag gtggaggaca tggcagagct 901 cacgtgcctc aacgaagcct cggtgctgca caacctcaag gagcgttact actcagggct 961 catctacacc tattcaggcc tgttctgtgt ggtcatcaat ccttacaaga acctgcccat 1021 ctactctgaa gagattgtgg aaatgtacaa gggcaagaag aggcacgaga tgccccctca 1081 catctatgcc atcacagaca ccgcctacag gagtatgatg caagaccgag aagatcaatc 1141 catcttgtgc actggtgaat ctggagctgg caagacggag aacaccaaga aggtcatcca 1201 gtatctggcg tacgtggcgt cctcgcacaa gagcaagaag gaccagggcg agctggagcg 1261 gcagctgctg caggccaacc ccatcctgga ggccttcggg aacgccaaga ccgtgaagaa 1321 tgacaactcc tcccgcttcg gcaaattcat tcgcatcaac tttgatgtca atggctacat 1381 tgttggagcc aacattgaga cttatctttt ggagaaatct cgtgctatcc gccaagccaa 1441 ggaagaacgg accttccaca tcttctatta tctcctgtct ggggctggag agcacctgaa 1501 gaccgatctc ctgttggagc cgtacaacaa ataccgcttc ctgtccaatg gacacgtcac 1561 catccccggg cagcaggaca aggacatgtt ccaggagacc atggaggcca tgaggattat 1621 gggcatccca gaagaggagc aaatgggcct gctgcgggtc atctcagggg ttcttcagct 1681 cggcaacatc gtcttcaaga aggagcggaa cactgaccag gcgtccatgc ccgacaacac 1741 agctgcccaa aaggtgtccc atctcttggg tatcaatgtg accgatttca ccagaggaat 1801 cctcaccccg cgcatcaagg tgggacggga ttacgtccag aaggcgcaga ctaaagagca 1861 ggctgacttt gccatcgagg ccttggccaa ggcgacctat gagcggatgt tccgctggct 1921 ggtgctgcgc atcaacaagg ctctggacaa gaccaagagg cagggcgcct ccttcatcgg 1981 gatcctggac attgccggct tcgagatctt tgatctgaac tcgtttgagc agctgtgcat 2041 caattacacc aatgagaagc tgcagcagct cttcaaccac accatgttca tcctggagca 2101 ggaggagtac cagcgcgagg gcatcgagtg gaacttcatc gactttggcc tcgacctgca 2161 gccctgcatc gacctcattg agaagccagc aggccccccg ggcattctgg ccctgctgga 2221 cgaggagtgc tggttcccca aagccaccga caagagcttc gtggagaagg tgatgcagga 2281 gcagggcacc caccccaagt tccagaagcc caagcagctg aaggacaaag ctgatttctg 2341 cattatccac tatgccggca aggtggatta caaagctgac gagtggctga tgaagaacat 2401 ggatcccctg aatgacaaca tcgccacact gctccaccag tcctctgaca agtttgtctc 2461 ggagctgtgg aaggatgtgg accgcatcat cggcctggac caggtggccg gcatgtcgga 2521 gaccgcactg cccggggcct tcaagacgcg gaagggcatg ttccgcactg tggggcagct 2581 ttacaaggag cagctggcca agctgatggc tacgctgagg aacacgaacc ccaactttgt 2641 ccgctgcatc atccccaacc acgagaagaa ggccggcaag ctggacccgc atctcgtgct 2701 ggaccagctg cgctgcaacg gtgttctcga gggcatccgt atctgccgcc agggcttccc 2761 caacagggtg gtcttccagg agtttcggca gagatatgag atcctgactc caaactccat 2821 tcccaagggt ttcatggacg ggaagcaggc gtgcgtgctc atgataaaag ccctggagct 2881 cgacagcaat ctgtaccgca ttggccagag caaagtcttc ttccgtgccg gtgtgctggc 2941 ccacctggag gaggagcgag acctgaagat caccgacgtc atcatagggt tccaggcctg 3001 ctgcaggggc tacctggcca ggaaagcatt tgccaagcgg cagcagcagc ttaccgccat 3061 gaaggtcctc cagcggaact gcgctgccta cctgaagctg cggaactggc agtggtggcg 3121 gctcttcacc aaggtcaagc cgctgctgca ggtgagccgg caggaggagg agatgatggc 3181 caaggaggag gagctggtga aggtcagaga gaagcagctg gctgcggaga acaggctcac 3241 ggagatggag acgctgcagt ctcagctcat ggcagagaaa ttgcagctgc aggagcagct 3301 ccaggcagaa accgagctgt gtgccgaggc tgaggagctc cgggcccgcc tgaccgccaa 3361 gaagcaggaa ttagaagaga tctgccatga cctagaggcc agggtggagg aggaggagga 3421 gcgctgccag cacctgcagg cggagaagaa gaagatgcag cagaacatcc aggagcttga 3481 ggagcagctg gaggaggagg agagcgcccg gcagaagctg cagctggaga aggtgaccac 3541 cgaggcgaag ctgaaaaagc tggaggagga gcagatcatc ctggaggacc agaactgcaa 3601 gctggccaag gaaaagaaac tgctggaaga cagaatagct gagttcacca ccaacctcac 3661 agaagaggag gagaaatcta agagcctcgc caagctcaag aacaagcatg aggcaatgat 3721 cactgacttg gaagagcgcc tccgcaggga ggagaagcag cgacaggagc tggagaagac 3781 ccgccggaag ctggagggag actccacaga cctcagcgac cagatcgccg agctccaggc 3841 ccagatcgcg gagctcaaga tgcagctggc caagaaagag gaggagctcc aggccgccct 3901 ggccagagtg gaagaggaag ctgcccagaa gaacatggcc ctcaagaaga tccgggagct 3961 ggaatctcag atctctgaac tccaggaaga cctggagtct gagcgtgctt ccaggaataa 4021 agctgagaag cagaaacggg accttgggga agagctagag gctctgaaaa cagagttgga 4081 ggacacgctg gattccacag ctgcccagca ggagctcagg tcaaaacgtg agcaggaggt 4141 gaacatcctg aagaagaccc tggaggagga ggccaagacc cacgaggccc agatccagga 4201 gatgaggcag aagcactcac aggccgtgga ggagctggcg gagcagctgg agcagacgaa 4261 gcgggtgaaa gcaaacctcg agaaggcaaa gcagactctg gagaacgagc ggggggagct 4321 ggccaacgag gtgaaggtgc tgctgcaggg caaaggggac tcggagcaca agcgcaagaa 4381 agtggaggcg cagctgcagg agctgcaggt caagttcaac gagggagagc gcgtgcgcac 4441 agagctggcc gacaaggtca ccaagctgca ggtggagctg gacaacgtga ccgggcttct 4501 cagccagtcc gacagcaagt ccagcaagct caccaaggac ttctccgcgc tggagtccca 4561 gctgcaggac actcaggagc tgctgcagga ggagaaccgg cagaagctga gcctgagcac 4621 caagctcaag caggtggagg acgagaagaa ttccttccgg gagcagctgg aggaggagga 4681 ggaggccaag cacaacctgg agaagcagat cgccaccctc catgcccagg tggccgacat 4741 gaaaaagaag atggaggaca gtgtggggtg cctggaaact gctgaggagg tgaagaggaa 4801 gctccagaag gacctggagg gcctgagcca gcggcacgag gagaaggtgg ccgcctacga 4861 caagctggag aagaccaaga cgcggctgca gcaggagctg gacgacctgc tggtggacct 4921 ggaccaccag cgccagagcg cgtgcaacct ggagaagaag cagaagaagt ttgaccagct 4981 cctggcggag gagaagacca tctctgccaa gtatgcagag gagcgcgacc gggctgaggc 5041 ggaggcccga gagaaggaga ccaaggctct gtcgctggcc cgggccctgg aggaagccat 5101 ggagcagaag gcggagctgg agcggctcaa caagcagttc cgcacggaga tggaggacct 5161 tatgagctcc aaggatgatg tgggcaagag tgtccacgag ctggagaagt ccaagcgggc 5221 cctagagcag caggtggagg agatgaagac gcagctggaa gagctggagg acgagctgca 5281 ggccaccgaa gatgccaagc tgcggttgga ggtcaacctg caggccatga aggcccagtt 5341 cgagcgggac ctgcagggcc gggacgagca gagcgaggag aagaagaagc agctggtcag 5401 acaggtgcgg gagatggagg cagagctgga ggacgagagg aagcagcgct cgatggcagt 5461 ggccgcccgg aagaagctgg agatggacct gaaggacctg gaggcgcaca tcgactcggc 5521 caacaagaac cgggacgaag ccatcaaaca gctgcggaag ctgcaggccc agatgaagga 5581 ctgcatgcgc gagctggatg acacccgcgc ctctcgtgag gagatcctgg cccaggccaa 5641 agagaacgag aagaagctga agagcatgga ggccgagatg atccagttgc aggaggaact 5701 ggcagccgcg gagcgtgcca agcgccaggc ccagcaggag cgggatgagc tggctgacga 5761 gatcgccaac agcagcggca aaggagccct ggcgttagag gagaagcggc gtctggaggc 5821 ccgcatcgcc cagctggagg aggagctgga ggaggagcag ggcaacacgg agctgatcaa 5881 cgaccggctg aagaaggcca acctgcagat cgaccagatc aacaccgacc tgaacctgga 5941 gcgcagccac gcccagaaga acgagaatgc tcggcagcag ctggaacgcc agaacaagga 6001 gcttaaggtc aagctgcagg agatggaggg cactgtcaag tccaagtaca aggcctccat 6061 caccgccctc gaggccaaga ttgcacagct ggaggagcag ctggacaacg agaccaagga 6121 gcgccaggca gcctgcaaac aggtgcgtcg gaccgagaag aagctgaagg atgtgctgct 6181 gcaggtggat gacgagcgga ggaacgccga gcagtacaag gaccaggccg acaaggcatc 6241 tacccgcctg aagcagctca agcggcagct ggaggaggcc gaagaggagg cccagcgggc 6301 caacgcctcc cgccggaaac tgcagcgcga gctggaggac gccactgaga cggccgatgc 6361 catgaaccgc gaagtcagct ccctaaagaa caagctcagg cgcggggacc tgccgtttgt 6421 cgtgccccgc cgaatggccc ggaaaggcgc cggggatggc tccgacgaag aggtagatgg 6481 caaagcggat ggggctgagg ccaaacctgc cgaaAAGCTT ACGCGTGACT ACAAAGACCA 6541 TGACGGTGAT TATAAAGATC ATGACATCGA CTACAAGGAT GACGATGACA AGTGAGGATC 6601 CGGGCCCGTT TAAACCCGCT GATCAGCCTC GACTGTGCCT TCTAGTTGCC AGCCATCTGT 6661 TGTTTGCCCC TCCCCCGTGC CTTCCTTGAC CCTGGAAGGT GCCACTCCCA CTGTCCTTTC 6721 CTAATAAAAT GAGGAAATTG CATCGCATTG TCTGAGTAGG TGTCATTCTA TTCTGGGGGG 6781 TGGGGTGGGG CAGGACAGCA AGGGGGAGGA TTGGGAAGAC AATAGCAGGC ATGCTGGGGA 6841 TGCGGTGGGC TCTATGGTCT AGAGATATAC TGAGTCATTA GGGACTTTCC AATGGGTTTT 6901 GCCCAGTACA TAAGGTCAAT AGGGGTGAAT CAACAGGAAA GTCCCATTGG AGCCAAGTAC 6961 ACTGAGTCAA TAGGGACTTT CCATTGGGTT TTGCCCAGTA CAAAAGGTCA ATAGGGGGTG 7021 AGTCAATGGG TTTTTCCCAT TATTGGCACG TACATAAGGT CAATAGGGGT GAGTCATTGG 7081 GTTTTTCCAG CCAaTTTAAT TAAAACGCCA TGTACTTTCC CACCATTGAC GTCAATGGGC 7141 TATTGAAACT AATGCAACGT GACCTTTAAA CGGTACTTTC CCATAGCTGA TTAATGGGAA 7201 AGTACCGTTC TCGAGCCAAT ACACGTCAAT GGGAAGTGAA AGGGCAGCCA AAACGTAACA 7261 CCGCCCCGGT TTTCCCCTGG AAATTCCATA TTGGCACGCA TTCTATTGGC TGAGCTGCGT 7321 TCTACGTGGG TATAAGAGGC GCGACCAGCG TCGGTACCGT CGCAGTCTTC GGTCTGACCA 7381 CCGTAGAACG CAGATCaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg 7441 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt 7501 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca 7561 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt 7621 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg 7681 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg 7741 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact 7801 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg 7861 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca 7921 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg 7981 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag 8041 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca 8101 ctctcggcat ggacgagctg tacaagtaaa gcggccgcga ctctagatca taatcagcca 8161 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct 8221 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta 8281 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag 8341 ttgtggtttg tccaaactca tcaatgtatc ttaaggcgta aattgtaagc gttaatattt 8401 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa 8461 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag 8521 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg 8581 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga 8641 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg 8701 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg 8761 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc 8821 cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt 8881 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc 8941 ttcaataata ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg 9001 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca 9061 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat 9121 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc 9181 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat 9241 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt 9301 ttttggaggc ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca 9361 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg 9421 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag 9481 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc 9541 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc 9601 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg 9661 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc 9721 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca 9781 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag 9841 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg 9901 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg 9961 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca 10021 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc 10081 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg 10141 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct 10201 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt 10261 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc 10321 ccaccctagg gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc 10381 tatgacggca ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg 10441 cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca 10501 atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg 10561 gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact 10621 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga 10681 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt 10741 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca 10801 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct 10861 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta 10921 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct 10981 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc 11041 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca 11101 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga 11161 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg 11221 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt 11281 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag 11341 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt 11401 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat 11461 gcat

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.