pCMV-3×FLAG-SREBF2-mCMV-EGFP-N

pCMV-3×FLAG-SREBF2-mCMV-EGFP-Neo plasmid

PVT95802

pCMV-3×FLAG-SREBF2-mCMV-EGFP-Neo plasmid Information

Plasmid name: pCMV-3×FLAG-SREBF2(human)-mCMV-EGFP-Neo
Alias: NM_004599.4;bHLHd2; SREBP-2; SREBP2
Gene lenght: 3426bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-3×FLAG-SREBF2-mCMV-EGFP-Neo plasmid Description

pCMV-3×FLAG-SREBF2-mCMV-EGFP-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-3×FLAG-SREBF2-mCMV-EGFP-Neo plasmid Sequence

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc GCTAGCGCTA 601 CCGGACTCAG ATCTCGAGCT CCGAATTCgc caccatggac tacaaagacc atgacggtga 661 ttataaagat catgacatcg actacaagga tgacgatgac aagggtggag gcggatcaat 721 ggacgacagc ggcgagctgg gtggtctgga gaccatggag accctcacgg agctgggcga 781 cgagctgacc ctgggagaca tcgacgagat gctgcaattt gtcagtaatc aagtgggaga 841 gttccctgac ttgttttcag aacagctgtg tagctccttt cctggcagtg gtggtagtgg 901 tagcagcagc ggcagcagtg gcagcagcag cagcagcagc aatggcaggg gcagcagcag 961 cggagctgtg gacccttcag tgcaacggtc attcacccag gtcacattac cttccttctc 1021 tccctcggcg gcctccccac aggctccaac tctgcaagtc aaggtttctc ccacctcagt 1081 tcccaccaca cccagggcaa ctcctattct tcagccccgc ccccagcccc agcctcaacc 1141 tcaaactcag ctgcaacaac agacggtaat gatcacgcca acattcagca ccactccgca 1201 gacgaggatc atccagcagc ctttgatata ccagaatgca gctactagct ttcaagtcct 1261 tcagcctcaa gtccaaagcc tggtgacatc ctcccaggta cagccggtca ccattcagca 1321 gcaggtgcag acagtacagg cccagcgggt gctgacacaa acggccaatg gcacgctgca 1381 gacccttgcc ccggctacgg tgcagacagt tgctgcgcca caggtgcagc aggtcccggt 1441 cctggtccag cctcagatca tcaagacaga ttcccttgtt ttgaccacac tgaagacaga 1501 tggcagccct gttatggctg cggtccagaa cccggccctc accgccctca ccacccctat 1561 ccagacggct gcccttcaag taccaaccct ggtgggcagc agtgggacca ttctgaccac 1621 aatgcctgta atgatggggc aagagaaagt gcccattaag caggtacctg ggggagtcaa 1681 gcagcttgag ccccccaaag aaggagaaag gcggacaacc cataatatca ttgagaaacg 1741 atatcgctcc tccatcaatg acaaaatcat cgaattgaaa gacctggtca tggggacaga 1801 cgccaagatg cacaagtctg gcgttctgag gaaggccatt gattacatca aatacttgca 1861 gcaggtcaat cataaactgc gccaggagaa catggtgctg aagctggcaa atcaaaagaa 1921 caagcttcta aagggcatcg acctaggcag tctggtggac aatgaggtgg acctgaagat 1981 cgaggacttt aatcagaatg tccttctgat gtccccccca gcctctgact cagggtccca 2041 ggctggcttc tctccctact ccattgactc tgagccagga agccctctat tggatgatgc 2101 aaaggtcaaa gatgagccag actctcctcc tgtggcgctg ggcatggtag accgctcacg 2161 gattcttctg tgtgtcctca ccttcctgtg cctctccttt aaccccctga cttccctgct 2221 gcagtgggga ggggcccacg actctgacca gcacccacac tcaggctctg gccgcagtgt 2281 cctgtcattc gagtcaggtt ctgggggctg gtttgactgg atgatgccta ctcttctctt 2341 atggctggta aatggtgtga ttgtcctgag cgtctttgtg aagctgctgg ttcatgggga 2401 gccagtgatc cggccacact cgcgctcctc ggtcaccttc tggaggcacc ggaaacaggc 2461 agatctggat ctcgccagag gagattttgc agctgctgcc ggcaacctac aaacctgcct 2521 ggcagttttg ggccgggcac tgcccacctc ccgcctggac ctggcctgca gcctctcctg 2581 gaacgtgatc cgctacagcc tgcagaagct acgcctggtg cgctggctgc tcaagaaagt 2641 cttccagtgc cggcgggcca cgccagccac tgaggcaggc tttgaagacg aagctaagac 2701 cagcgcccgg gatgcggctc tggcctatca ccggctgcac cagctgcaca tcacagggaa 2761 gcttcctgca ggatccgcct gttccgatgt acacatggcg ttgtgtgccg tgaacctggc 2821 tgaatgtgca gaggagaaga tcccaccgag cacactggtt gagatccatc tgactgctgc 2881 catggggctc aagacccggt gtggaggcaa gctgggcttc ctggccagct acttcctcag 2941 ccgagcccag agcctgtgtg gccccgagca cagtgctgtt cctgactccc tgcgctggct 3001 ctgccacccc ctgggccaga agtttttcat ggagcggagc tggtctgtga agtcagctgc 3061 caaggagagt ctatactgtg cccagaggaa cccagctgac cccattgcgc aggtccacca 3121 ggccttctgc aagaacctgc tggagcgagc tatagagtcc ttggtgaaac ctcaggccaa 3181 gaagaaggct ggagaccagg aagaagagag ctgtgaattc tccagtgctc tggagtactt 3241 gaaattactt cattcttttg tggactctgt gggggttatg agccccccac tctccaggag 3301 ctccgtgctc aagtccgccc tgggtccaga catcatctgt cggtggtgga cgtctgcaat 3361 cactgtggcc atcagctggc tccagggaga cgatgcagct gtgcgctctc attttaccaa 3421 agtggaacgc atccccaagg ccctggaagt gacagagagc cccctggtga aggccatctt 3481 ccatgcctgc agagccatgc atgcctcact ccctgggaaa gcagatgggc agcagagttc 3541 cttctgccat tgcgagaggg ccagtggcca cctatggagc agcctcaacg tcagtggggc 3601 cacctctgac cctgccctca accacgtggt ccagctgctc acctgtgacc tgctactgtc 3661 gctacggaca gcgctctggc aaaaacaggc cagtgccagc caggctgtgg gggagaccta 3721 ccacgcgtca ggcgctgaac tggcgggctt ccaacgggac ctgggcagcc tgcgcaggct 3781 ggcacacagc ttccgcccag cataccgcaa ggtgttcctg catgaagcca ccgtgcgcct 3841 gatggcagga gccagcccca cccgcaccca ccagctgctg gaacacagcc tgcggcggcg 3901 caccacgcag agcaccaagc acggagaggt ggatgcctgg cccggccagc gagagcgggc 3961 caccgccatc ctgctggcct gccgccacct gcccctctcc ttcctctcct ccccgggcca 4021 gcgggcagtg ctgctggccg aagctgcccg caccctggag aaggtgggcg accggcgctc 4081 ctgcaacgac tgccagcaga tgattgttaa gctgggtggt ggcactgcca ttgccgcctc 4141 ctgaGGATCC GGGCCCGTTT AAACCCGCTG ATCAGCCTCG ACTGTGCCTT CTAGTTGCCA 4201 GCCATCTGTT GTTTGCCCCT CCCCCGTGCC TTCCTTGACC CTGGAAGGTG CCACTCCCAC 4261 TGTCCTTTCC TAATAAAATG AGGAAATTGC ATCGCATTGT CTGAGTAGGT GTCATTCTAT 4321 TCTGGGGGGT GGGGTGGGGC AGGACAGCAA GGGGGAGGAT TGGGAAGACA ATAGCAGGCA 4381 TGCTGGGGAT GCGGTGGGCT CTATGGTCTA GAGATATACT GAGTCATTAG GGACTTTCCA 4441 ATGGGTTTTG CCCAGTACAT AAGGTCAATA GGGGTGAATC AACAGGAAAG TCCCATTGGA 4501 GCCAAGTACA CTGAGTCAAT AGGGACTTTC CATTGGGTTT TGCCCAGTAC AAAAGGTCAA 4561 TAGGGGGTGA GTCAATGGGT TTTTCCCATT ATTGGCACGT ACATAAGGTC AATAGGGGTG 4621 AGTCATTGGG TTTTTCCAGC CAaTTTAATT AAAACGCCAT GTACTTTCCC ACCATTGACG 4681 TCAATGGGCT ATTGAAACTA ATGCAACGTG ACCTTTAAAC GGTACTTTCC CATAGCTGAT 4741 TAATGGGAAA GTACCGTTCT CGAGCCAATA CACGTCAATG GGAAGTGAAA GGGCAGCCAA 4801 AACGTAACAC CGCCCCGGTT TTCCCCTGGA AATTCCATAT TGGCACGCAT TCTATTGGCT 4861 GAGCTGCGTT CTACGTGGGT ATAAGAGGCG CGACCAGCGT CGGTACCGTC GCAGTCTTCG 4921 GTCTGACCAC CGTAGAACGC AGATCaccgg tcgccaccat ggtgagcaag ggcgaggagc 4981 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt 5041 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca 5101 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg 5161 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg 5221 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca 5281 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg 5341 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca 5401 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga 5461 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc 5521 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc 5581 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg 5641 ccgggatcac tctcggcatg gacgagctgt acaagtaaag cggccgcgac tctagatcat 5701 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc 5761 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta 5821 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 5881 gcattctagt tgtggtttgt ccaaactcat caatgtatct taaggcgtaa attgtaagcg 5941 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat 6001 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg 6061 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc 6121 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt 6181 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag 6241 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg 6301 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc 6361 ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc 6421 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct 6481 gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg 6541 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca 6601 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg 6661 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc 6721 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat 6781 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg 6841 aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat 6901 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga 6961 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc 7021 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga 7081 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg 7141 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc 7201 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg 7261 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga 7321 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc 7381 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca 7441 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg 7501 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct 7561 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg 7621 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc 7681 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac 7741 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt 7801 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga 7861 gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg 7921 aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt 7981 tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca 8041 ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga 8101 aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc 8161 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat 8221 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc 8281 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg 8341 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct 8401 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct 8461 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct 8521 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg 8581 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc 8641 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga 8701 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg 8761 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta 8821 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg 8881 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg 8941 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat 9001 taccgccatg cat

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.