pCMV-mEGFP-DDX3X-Neo plasmid

pCMV-mEGFP-DDX3X-Neo plasmid

PVT96770

pCMV-mEGFP-DDX3X-Neo plasmid Information

Plasmid name: pCMV-mEGFP-DDX3X(human)-Neo
Alias: NM_001356.5;CAP-Rf; DBX; DDX14; DDX3; HLP2; MRX102;MRXSSB
Gene lenght: 1985bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: pEGFP-C-5'
Reverse primer:
Copy number: High
Induction:

pCMV-mEGFP-DDX3X-Neo plasmid Description

pCMV-mEGFP-DDX3X-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-mEGFP-DDX3X-Neo plasmid Sequence

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg 661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc 721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg 781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc 841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag 901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag 961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac 1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac 1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc 1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg 1201 cccgacaacc actacctgag cacccagtcc aagctgagca aagaccccaa cgagaagcgc 1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag 1321 ctgtacaagt ccggactcag atctcgaggt ggaggtggat caggtggtgg aggatctaag 1381 cttctgcgtc gagaattcat gagtcatgtg gcagtggaaa atgcgctcgg gctggaccag 1441 cagtttgctg gcctagacct gaactcttca gataatcaga gtggaggaag tacagccagc 1501 aaagggcgct atattcctcc tcatttaagg aaccgagaag ctactaaagg tttctacgat 1561 aaagacagtt cagggtggag ttctagcaaa gataaggatg cgtatagcag ttttggatct 1621 cgtagtgatt caagagggaa gtctagcttc ttcagtgatc gtggaagtgg atcaagggga 1681 aggtttgatg atcgtggacg gagtgattac gatggcattg gcagccgtgg tgacagaagt 1741 ggctttggca aatttgaacg tggtggaaac agtcgctggt gtgacaaatc agatgaagat 1801 gattggtcaa aaccactccc accaagtgaa cgcttggaac aggaactctt ttctggaggc 1861 aacactggga ttaattttga gaaatacgat gacattccag ttgaggcaac aggcaacaac 1921 tgtcctccac atattgaaag tttcagtgat gttgagatgg gagaaattat catgggaaac 1981 attgagctta ctcgttatac tcgcccaact ccagtgcaaa agcatgctat tcctattatc 2041 aaagagaaaa gagacttgat ggcttgtgcc caaacagggt ctggaaaaac tgcagcattt 2101 ctgttgccca tcttgagtca gatttattca gatggtccag gcgaggcttt gagggccatg 2161 aaggaaaatg gaaggtatgg gcgccgcaaa caatacccaa tctccttggt attagcacca 2221 acgagagagt tggcagtaca gatctacgag gaagccagaa aattttcata ccgatctaga 2281 gttcgtcctt gcgtggttta tggtggtgcc gatattggtc agcagattcg agacttggaa 2341 cgtggatgcc atttgttagt agccactcca ggacgtctag tggatatgat ggaaagagga 2401 aagattggat tagacttttg caaatacttg gtgttagatg aagctgatcg gatgttggat 2461 atggggtttg agcctcagat tcgtagaata gtcgaacaag atactatgcc tccaaagggt 2521 gtccgccaca ctatgatgtt tagtgctact tttcctaagg aaatacagat gctggctcgt 2581 gatttcttag atgaatatat cttcttggct gtaggaagag ttggctctac ctctgaaaac 2641 atcacacaga aagtagtttg ggtggaagaa tcagacaaac ggtcatttct gcttgacctc 2701 ctaaatgcaa caggcaagga ttcactgacc ttagtgtttg tggagaccaa aaagggtgca 2761 gattctctgg aggatttctt ataccatgaa ggatacgcat gtaccagcat ccatggagac 2821 cgttctcaga gggatagaga agaggccctt caccagttcc gctcaggaaa aagcccaatt 2881 ttagtggcta cagcagtagc agcaagagga ctggacattt caaatgtgaa acatgttatc 2941 aattttgact tgccaagtga tattgaagaa tatgtacatc gtattggtcg tacgggacgt 3001 gtaggaaacc ttggcctggc aacctcattc tttaacgaga ggaacataaa tattactaag 3061 gatttgttgg atcttcttgt tgaagctaaa caagaagtgc cgtcttggtt agaaaacatg 3121 gcttatgaac accactacaa gggtagcagt cgtggacgtt ctaagagtag cagatttagt 3181 ggagggtttg gtgccagaga ctaccgacaa agtagcggtg ccagcagttc cagcttcagc 3241 agcagccgcg caagcagcag ccgcagtggc ggaggtggcc acggtagcag cagaggattt 3301 ggtggaggtg gctatggagg cttttacaac agtgatggat atggaggaaa ttataactcc 3361 cagggggttg actggtgggg taactgagga tccaccggat ctagataact gatcataatc 3421 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg 3481 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat 3541 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat 3601 tctagttgtg gtttgtccaa actcatcaat gtatcttaac gcgtaaattg taagcgttaa 3661 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc 3721 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt 3781 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa 3841 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg 3901 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg 3961 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc 4021 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa 4081 tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat 4141 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata 4201 aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat 4261 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc 4321 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga 4381 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc 4441 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 4501 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga 4561 ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt 4621 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct 4681 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct 4741 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga 4801 actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc 4861 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg 4921 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc 4981 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca 5041 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga 5101 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc 5161 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga 5221 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca 5281 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg 5341 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct 5401 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc 5461 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga 5521 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc 5581 ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc 5641 cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat 5701 aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg 5761 ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc 5821 ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat 5881 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 5941 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 6001 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 6061 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 6121 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta 6181 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 6241 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 6301 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 6361 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 6421 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 6481 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 6541 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 6601 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 6661 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc 6721 gccatgcat

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.