pLV3-H1-SOX9-shRNA3-CopGFP-Pur

pLV3-H1-SOX9-shRNA3-CopGFP-Puro plasmid

PVT93428

pLV3-H1-SOX9-shRNA3-CopGFP-Puro plasmid Information

Plasmid name: pLV3-H1-SOX9(human)-shRNA3-CopGFP-Puro
Alias: GCAGCGACGTCATCTCCAA
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Interference silence
Fragment type: shRNA
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: H1
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: H1
Reverse primer:
Copy number: High
Induction:

pLV3-H1-SOX9-shRNA3-CopGFP-Puro plasmid Description

pLV3-H1-SOX9-shRNA3-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-H1-SOX9-shRNA3-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtGAACGCTG ACGTCATCAA CCCGCTCCAA GGAATCGCGG GCCCAGTGTC 1981 ACTAGGCGGG AACACCCAGC GCGCGTGCGC CcTGGCAGGA AGATGGCTGT GAGGGACAGG 2041 GGAGTGGCGC CCTGCAATAT TTGCATGTCG CTATGTGTTC TGGGAAATCA CCATAAACGT 2101 GAAATGTCTT TGGATTTGGG AATCTTATAA GTTCTGTATG AGACCACTCA TCgctagcga 2161 attcgGCAGC GACGTCATCT CCAAttcaag agaTTGGAGA TGACGTCGCT GCTTTTTTgg 2221 atccgcggcc gcGaaggatc tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat 2281 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt gcctagagaa 2341 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg 2401 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt 2461 ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca cgcgcccgcc 2521 gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg 2581 gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg agaccgggcc 2641 tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg ctttgcctga 2701 ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt tacagatcca 2761 agctgtgacc ggcgcctacg ctagacgcca ccatggagag cgacgagagc ggcctgcccg 2821 ccatggagat cgagtgccgc atcaccggca ccctgaacgg cgtggagttc gagctggtgg 2881 gcggcggaga gggcaccccc aagcagggcc gcatgaccaa caagatgaag agcaccaaag 2941 gcgccctgac cttcagcccc tacctgctga gccacgtgat gggctacggc ttctaccact 3001 tcggcaccta ccccagcggc tacgagaacc ccttcctgca cgccatcaac aacggcggct 3061 acaccaacac ccgcatcgag aagtacgagg acggcggcgt gctgcacgtg agcttcagct 3121 accgctacga ggccggccgc gtgatcggcg acttcaaggt ggtgggcacc ggcttccccg 3181 aggacagcgt gatcttcacc gacaagatca tccgcagcaa cgccaccgtg gagcacctgc 3241 accccatggg cgataacgtg ctggtgggca gcttcgcccg caccttcagc ctgcgcgacg 3301 gcggctacta cagcttcgtg gtggacagcc acatgcactt caagagcgcc atccacccca 3361 gcatcctgca gaacgggggc cccatgttcg ccttccgccg cgtggaggag ctgcacagca 3421 acaccgagct gggcatcgtg gagtaccagc acgccttcaa gacccccatc gccttcgcca 3481 gatcccgcgc tcagtcgtcc aattctgccg tggacggcac cgccggaccc ggctccaccg 3541 gatctcgcga gggcagagga agtcttctaa catgcggtga cgtggaggag aatcccggcc 3601 ctatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg 3661 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg 3721 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg 3781 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg 3841 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg 3901 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg 3961 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg 4021 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc 4081 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg 4141 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct 4201 gaaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg 4261 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc 4321 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt 4381 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca 4441 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc 4501 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc 4561 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc 4621 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc 4681 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc 4741 tccgccttcg ccctcagacg agtcggatct ccctttggcc gcctccccgc ctggtacctt 4801 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg 4861 gactggaagg gctaattcac tcccaacgaa aataagatct gctttttgct tgtactgggt 4921 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc 4981 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg 5041 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta 5101 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga 5161 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa 5221 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca 5281 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca gttccgccca 5341 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc 5401 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctagact tttgcagaga 5461 cggcccaaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 5521 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 5581 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 5641 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 5701 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 5761 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 5821 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 5881 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 5941 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 6001 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 6061 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 6121 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 6181 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 6241 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 6301 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 6361 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 6421 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 6481 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 6541 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 6601 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 6661 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 6721 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 6781 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 6841 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 6901 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 6961 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 7021 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 7081 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 7141 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 7201 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 7261 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 7321 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 7381 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 7441 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 7501 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 7561 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 7621 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag 7681 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca 7741 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc 7801 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc 7861 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag 7921 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg 7981 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg 8041 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc 8101 caagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.