pTac-GST-TEV-G3BP1 plasmid

pTac-GST-TEV-G3BP1 plasmid

PVT96738

pTac-GST-TEV-G3BP1 plasmid Information

Plasmid name: pTac-GST-TEV-G3BP1(human)
Alias: NM_198395.2;G3BP; HDH-VIII
Gene lenght: 1401bp
Host: E.coli
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: Tac
Replicon: pBR322
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: GST-F2
Reverse primer:
Copy number: High
Induction:

pTac-GST-TEV-G3BP1 plasmid Description

pTac-GST-TEV-G3BP1 is a plasmid suitable for protein expression in Escherichia coli.

pTac-GST-TEV-G3BP1 plasmid Sequence

1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg 61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt 121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc 181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca 241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc 301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc 361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc 421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata 481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc 541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact 601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag 661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt 721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa 781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat 841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc 901 atcctccaaa atcggatgag aatctctact tccagggaat ggtgatggag aagcctagtc 961 ccctgctggt cgggcgggaa tttgtgagac agtattacac actgctgaac caggccccag 1021 acatgctgca tagattttat ggaaagaact cttcttatgt ccatggggga ttggattcaa 1081 atggaaagcc agcagatgca gtctacggac agaaagaaat ccacaggaaa gtgatgtcac 1141 aaaacttcac caactgccac accaagattc gccatgttga tgctcatgcc acgctaaatg 1201 atggtgtggt agtccaggtg atggggcttc tctctaacaa caaccaggct ttgaggagat 1261 tcatgcaaac gtttgtcctt gctcctgagg ggtctgttgc aaataaattc tatgttcaca 1321 atgatatctt cagataccaa gatgaggtct ttggtgggtt tgtcactgag cctcaggagg 1381 agtctgaaga agaagtagag gaacctgaag aaagacagca aacacctgag gtggtacctg 1441 atgattctgg aactttctat gatcaggcag ttgtcagtaa tgacatggaa gaacatttag 1501 aggagcctgt tgctgaacca gagcctgatc ctgaaccaga accagaacaa gaacctgtat 1561 ctgaaatcca agaggaaaag cctgagccag tattagaaga aactgcccct gaggatgctc 1621 agaagagttc ttctccagca cctgcagaca tagctcagac agtacaggaa gacttgagga 1681 cattttcttg ggcatctgtg accagtaaga atcttccacc cagtggagct gttccagtta 1741 ctgggatacc acctcatgtt gttaaagtac cagcttcaca gccccgtcca gagtctaagc 1801 ctgaatctca gattccacca caaagacctc agcgggatca aagagtgcga gaacaacgaa 1861 taaatattcc tccccaaagg ggacccagac caatccgtga ggctggtgag caaggtgaca 1921 ttgaaccccg aagaatggtg agacaccctg acagtcacca actcttcatt ggcaacctgc 1981 ctcatgaagt ggacaaatca gagcttaaag atttctttca aagttatgga aacgtggtgg 2041 agttgcgcat taacagtggt gggaaattac ccaattttgg ttttgttgtg tttgatgatt 2101 ctgagcctgt tcagaaagtc cttagcaaca ggcccatcat gttcagaggt gaggtccgtc 2161 tgaatgtcga agagaagaag actcgagctg ccagggaagg cgaccgacga gataatcgcc 2221 ttcggggacc tggaggccct cgaggtgggc tgggtggtgg aatgagaggc cctccccgtg 2281 gaggcatggt gcagaaacca ggatttggag tgggaagggg gcttgcgcca cggcagtgac 2341 tcgagcggcc gcatcgtgac tgactgacga tctgcctcgc gcgtttcggt gatgacggtg 2401 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg 2461 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca 2521 tgacccagtc acgtagcgat agcggagtgt ataattcttg aagacgaaag ggcctcgtga 2581 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca 2641 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata 2701 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga 2761 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc 2821 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg 2881 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc 2941 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat 3001 cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact 3061 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat 3121 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga 3181 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc 3241 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga 3301 tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag 3361 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc 3421 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt 3481 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct 3541 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg 3601 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg 3661 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca 3721 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga 3781 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa 3841 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga 3901 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt 3961 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt 4021 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat 4081 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct 4141 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca 4201 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag 4261 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc 4321 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga 4381 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca 4441 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag 4501 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg 4561 aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcataa 4621 attccgacac catcgaatgg tgcaaaacct ttcgcggtat ggcatgatag cgcccggaag 4681 agagtcaatt cagggtggtg aatgtgaaac cagtaacgtt atacgatgtc gcagagtatg 4741 ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca ggccagccac gtttctgcga 4801 aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa ttacattccc aaccgcgtgg 4861 cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt tgccacctcc agtctggccc 4921 tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg cgccgatcaa ctgggtgcca 4981 gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaagcg gcggtgcaca 5041 atcttctcgc gcaacgcgtc agtgggctga tcattaacta tccgctggat gaccaggatg 5101 ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt atttcttgat gtctctgacc 5161 agacacccat caacagtatt attttctccc atgaagacgg tacgcgactg ggcgtggagc 5221 atctggtcgc attgggtcac cagcaaatcg cgctgttagc gggcccatta agttctgtct 5281 cggcgcgtct gcgtctggct ggctggcata aatatctcac tcgcaatcaa attcagccga 5341 tagcggaacg ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc atgcaaatgc 5401 tgaatgaggg catcgttccc actgcgatgc tggttgccaa cgatcagatg gcgctgggcg 5461 caatgcgcgc cattaccgag tccgggctgc gcgttggtgc ggatatctcg gtagtgggat 5521 acgacgatac cgaagacagc tcatgttata tcccgccgtt aaccaccatc aaacaggatt 5581 ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca actctctcag ggccaggcgg 5641 tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc ctggcgccca 5701 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg 5761 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat 5821 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc 5881 ggataacaat ttcacacagg aaacagctat gaccatgatt acggattcac tggccgtcgt 5941 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca 6001 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca 6061 gttgcgcagc ctgaatggcg aatggcgctt tgcctggttt ccggcaccag aagcggtgcc 6121 ggaaagctgg ctggagtgcg atcttcctga ggccgatact gtcgtcgtcc cctcaaactg 6181 gcagatgcac ggttacgatg cgcccatcta caccaacgta acctatccca ttacggtcaa 6241 tccgccgttt gttcccacgg agaatccgac gggttgttac tcgctcacat ttaatgttga 6301 tgaaagctgg ctacaggaag gccagacgcg aattattttt gatggcgttg gaatt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.