pCMV-LagBit-AP2A1 plasmid

pCMV-LagBit-AP2A1 plasmid

PVT96728

pCMV-LagBit-AP2A1 plasmid Information

Plasmid name: pCMV-LagBit-AP2A1(human)
Alias: NM_014203.3;ADTAA; AP2-ALPHA; CLAPA1
Gene lenght: 2934bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: LagBit-F1
Reverse primer:
Copy number: High
Induction:

pCMV-LagBit-AP2A1 plasmid Description

pCMV-LagBit-AP2A1 is a plasmid suitable for protein expression in mammalian cells.

pCMV-LagBit-AP2A1 plasmid Sequence

1 TTCGAGCTCG CCCGACATTG ATTATTGACT AGTTATTAAT AGTAATCAAT TACGGGGTCA 61 TTAGTTCATA GCCCATATAT GGAGTTCCGC GTTACATAAC TTACGGTAAA TGGCCCGCCT 121 GGCTGACCGC CCAACGACCC CCGCCCATTG ACGTCAATAA TGACGTATGT TCCCATAGTA 181 ACGCCAATAG GGACTTTCCA TTGACGTCAA TGGGTGGAGT ATTTACGGTA AACTGCCCAC 241 TTGGCAGTAC ATCAAGTGTA TCATATGCCA AGTACGCCCC CTATTGACGT CAATGACGGT 301 AAATGGCCCG CCTGGCATTA TGCCCAGTAC ATGACCTTAT GGGACTTTCC TACTTGGCAG 361 TACATCTACG TATTAGTCAT CGCTATTACC ATGGTGATGC GGTTTTGGCA GTACATCAAT 421 GGGCGTGGAT AGCGGTTTGA CTCACGGGGA TTTCCAAGTC TCCACCCCAT TGACGTCAAT 481 GGGAGTTTGT TTTGGCACCA AAATCAACGG GACTTTCCAA AATGTCGTAA CAACTCCGCC 541 CCATTGACGC AAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG CAGAGCTCGT 601 TTAGTGAACC GTCAGATCGC CTGGAGACGC CATCCACGCT GTTTTGACCT CCATAGAAGA 661 CACCGGGACC GATCCAGCCT CCGCGGCCGG GAACGGTGCA TTGGAACGCG GATTCCCCGT 721 GCCAAGAGTG ACGTAAGTAC CGCCTATAGA GTCTATAGGC CCACCCCCTT GGCTTCGTTA 781 GAACGCGGCT ACAATTAATA CATAACCTTA TGTATCATAC ACATACGATT TAGGTGACAC 841 TATAGAATAA CATCCACTTT GCCTTTCTCT CCACAGGTGT CCACTCCCAG GTCCAACTGC 901 ACCTCGGTTC TATCGATTGA ATTCATGGTC TTCACACTCG AAGATTTCGT TGGGGACTGG 961 GAACAGACAG CCGCCTACAA CCTGGACCAA GTCCTTGAAC AGGGAGGTGT GTCCAGTTTG 1021 CTGCAGAATC TCGCCGTGTC CGTAACTCCG ATCCAAAGGA TTGTCCGGAG CGGTGAAAAT 1081 GCCCTGAAGA TCGACATCCA TGTCATCATC CCGTATGAAG GTCTGAGCGC CGACCAAATG 1141 GCCCAGATCG AAGAGGTGTT TAAGGTGGTG TACCCTGTGG ATGATCATCA CTTTAAGGTG 1201 ATCCTGCCCT ATGGCACACT GGTAATCGAC GGGGTTACGC CGAACATGCT GAACTATTTC 1261 GGACGGCCGT ATGAAGGCAT CGCCGTGTTC GACGGCAAAA AGATCACTGT AACAGGGACC 1321 CTGTGGAACG GCAACAAAAT TATCGACGAG CGCCTGATCA CCCCCGACGG CTCCATGCTG 1381 TTCCGAGTAA CCATCAACAG TAGCGGTGGA GGCGGTTCAG GAGGTGGCGG ATCCatgccg 1441 gccgtgtcca agggcgatgg gatgcggggg ctcgcggtgt tcatctccga catccggaac 1501 tgtaagagca aagaggcgga aattaagaga atcaacaagg aactggccaa catccgctcc 1561 aagttcaaag gagacaaagc cttggatggc tacagtaaga aaaaatatgt gtgtaaactg 1621 cttttcatct tcctgcttgg ccatgacatt gactttgggc acatggaggc tgtgaatctg 1681 ttgagttcca ataaatacac agagaagcaa ataggttacc tgttcatttc tgtgctggtg 1741 aactcgaact cggagctgat ccgcctcatc aacaacgcca tcaagaatga cctggccagc 1801 cgcaacccca ccttcatgtg cctggccctg cactgcatcg ccaacgtggg cagccgggag 1861 atgggcgagg cctttgccgc tgacatcccc cgcatcctgg tggccgggga cagcatggac 1921 agtgtcaagc agagtgcggc cctgtgcctc cttcgactgt acaaggcctc gcctgacctg 1981 gtgcccatgg gcgagtggac ggcgcgtgtg gtacacctgc tcaatgacca gcacatgggt 2041 gtggtcacgg ccgccgtcag cctcatcacc tgtctctgca agaagaaccc agatgacttc 2101 aagacgtgcg tctctctggc tgtgtcgcgc ctgagccgga tcgtctcctc tgcctccacc 2161 gacctccagg actacaccta ctacttcgtc ccagcaccct ggctctcggt gaagctcctg 2221 cggctgctgc agtgctaccc gcctccagag gatgcggctg tgaaggggcg gctggtggaa 2281 tgtctggaga ctgtgctcaa caaggcccag gagcccccca aatccaagaa ggtgcagcat 2341 tccaacgcca agaacgccat cctcttcgag accatcagcc tcatcatcca ctatgacagt 2401 gagcccaacc tcctggttcg ggcctgcaac cagctgggcc agttcctgca gcaccgggag 2461 accaacctgc gctacctggc cctggagagc atgtgcacgc tggccagctc cgagttctcc 2521 catgaagccg tcaagacgca cattgacacc gtcatcaatg ccctcaagac ggagcgggac 2581 gtcagcgtgc ggcagcgggc ggctgacctc ctctacgcca tgtgtgaccg gagcaatgcc 2641 aagcagatcg tgtcggagat gctgcggtac ctggagacgg cagactacgc catccgcgag 2701 gagatcgtcc tgaaggtggc catcctggcc gagaagtacg ccgtggacta cagctggtac 2761 gtggacacca tcctcaacct catccgcatt gcgggcgact acgtgagtga ggaggtgtgg 2821 taccgtgtgc tacagatcgt caccaaccgt gatgacgtcc agggctatgc cgccaagacc 2881 gtctttgagg cgctccaggc ccctgcctgt cacgagaaca tggtgaaggt tggcggctac 2941 atccttgggg agtttgggaa cctgattgct ggggaccccc gctccagccc cccagtgcag 3001 ttctccctgc tccactccaa gttccatctg tgcagcgtgg ccacgcgggc gctgctgctg 3061 tccacctaca tcaagttcat caacctcttc cccgagacca aggccaccat ccagggcgtc 3121 ctgcgggccg gctcccagct gcgcaatgct gacgtggagc tgcagcagcg agccgtggag 3181 tacctcaccc tcagctcagt ggccagcacc gacgtcctgg ccacggtgct ggaggagatg 3241 ccgcccttcc ccgagcgcga gtcgtccatc ctggccaagc tgaaacgcaa gaaggggcca 3301 ggggccggca gcgccctgga cgatggccgg agggacccca gcagcaacga catcaacggg 3361 ggcatggagc ccacccccag cactgtgtcg acgccctcgc cctccgccga cctcctgggg 3421 ctgcgggcag cccctccccc ggcagcaccc ccggcttctg caggagcagg gaaccttctg 3481 gtggacgtct tcgatggccc ggccgcccag cccagcctgg ggcccacccc cgaggaggcc 3541 ttcctcagcg agctggagcc gcctgccccc gagagcccca tggctttgct ggctgaccca 3601 gctccagctg ctgacccagg tcctgaggac atcggccctc ccattccgga agccgatgag 3661 ttgctgaata agtttgtgtg taagaacaac ggggtcctgt tcgagaacca gctgctgcag 3721 atcggagtca agtcagagtt ccgacagaac ctgggccgca tgtatctctt ctatggcaac 3781 aagacctcgg tgcagttcca gaatttctca cccactgtgg ttcacccggg agacctccag 3841 actcagctgg ctgtgcagac caagcgcgtg gcggcgcagg tggacggcgg cgcgcaggtg 3901 cagcaggtgc tcaatatcga gtgcctgcgg gacttcctga cgcccccgct gctgtccgtg 3961 cgcttccggt acggtggcgc cccccaggcc ctcaccctga agctcccagt gaccatcaac 4021 aagttcttcc agcccaccga gatggcggcc caggatttct tccagcgctg gaagcagctg 4081 agcctccctc aacaggaggc gcagaaaatc ttcaaagcca accaccccat ggacgcagaa 4141 gttactaagg ccaagcttct ggggtttggc tctgctctcc tggacaatgt ggaccccaac 4201 cctgagaact tcgtgggggc ggggatcatc cagactaaag ccctgcaggt gggctgtctg 4261 cttcggctgg agcccaatgc ccaggcccag atgtaccggc tgaccctgcg caccagcaag 4321 gagcccgtct cccgtcacct gtgtgagctg ctggcacagc agttctgaAA GCTTGGCCGC 4381 CATGGCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA 4441 ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA 4501 ATGTATCTTA TCATGTCTGG ATCGGGAATT AATTCGGCGC AGCACCATGG CCTGAAATAA 4561 CCTCTGAAAG AGGAACTTGG TTAGGTACCT TCTGAGGCGG AAAGAACCAG CTGTGGAATG 4621 TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA 4681 TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA 4741 GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA 4801 TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT 4861 TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG 4921 GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTGTTAAC AGCTTGGCAC TGGCCGTCGT 4981 TTTACAACGT CGTGACTGGG AAAACCCTGG CGTTACCCAA CTTAATCGCC TTGCAGCACA 5041 TCCCCCTTTC GCCAGCTGGC GTAATAGCGA AGAGGCCCGC ACCGATCGCC CTTCCCAACA 5101 GTTGCGCAGC CTGAATGGCG AATGGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG 5161 CGGTATTTCA CACCGCATAC GTCAAAGCAA CCATAGTACG CGCCCTGTAG CGGCGCATTA 5221 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 5281 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 5341 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 5401 AAAAAACTTG ATTTGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 5461 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 5521 ACACTCAACC CTATCTCGGG CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 5581 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTTAA CAAAATATTA 5641 ACGTTTACAA TTTTATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC 5701 CAGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA 5761 TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG 5821 TCATCACCGA AACGCGCGAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT 5881 GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA 5941 ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA 6001 CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT 6061 GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG 6121 CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG 6181 GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG 6241 AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG 6301 CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA 6361 GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG 6421 AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC 6481 GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG 6541 AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG 6601 TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC 6661 TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG 6721 TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG 6781 GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT 6841 ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA 6901 CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT 6961 AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG 7021 TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT 7081 TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT 7141 TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG 7201 CAGATACCAA ATACTGTTCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT 7261 GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC 7321 GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG 7381 TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA 7441 CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG 7501 GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG 7561 GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA 7621 TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT 7681 TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT 7741 GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA 7801 ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG 7861 CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG 7921 AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG 7981 GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT 8041 CACACAGGAA ACAGCTATGA CATGATTACG AATTAA

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.