pLV2-EF1a-Myc-Opa1-IRES-Puro p

pLV2-EF1a-Myc-Opa1-IRES-Puro plasmid

PVT89209

pLV2-EF1a-Myc-Opa1-IRES-Puro plasmid Information

Plasmid name: pLV2-EF1a-Myc-Opa1(mouse)-IRES-Puro
Alias: NM_001403170.1;1200011N24Rik; lilr3; mKIAA0567
Gene lenght: 3048bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: EF1a
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: PEF-F
Reverse primer:
Copy number: High
Induction:

pLV2-EF1a-Myc-Opa1-IRES-Puro plasmid Description

pLV2-EF1a-Myc-Opa1-IRES-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV2-EF1a-Myc-Opa1-IRES-Puro plasmid Sequence

1 TGGAAGGGCT AATTCACTCC CAAAGAAGAC AAGATATCCT TGATCTGTGG ATCTACCACA 61 CACAAGGCTA CTTCCCTGAT TAGCAGAACT ACACACCAGG GCCAGGGGTC AGATATCCAC 121 TGACCTTTGG ATGGTGCTAC AAGCTAGTAC CAGTTGAGCC AGATAAGGTA GAAGAGGCCA 181 ATAAAGGAGA GAACACCAGC TTGTTACACC CTGTGAGCCT GCATGGGATG GATGACCCGG 241 AGAGAGAAGT GTTAGAGTGG AGGTTTGACA GCCGCCTAGC ATTTCATCAC GTGGCCCGAG 301 AGCTGCATCC GGAGTACTTC AAGAACTGCT GATATCGAGC TTGCTACAAG GGACTTTCCG 361 CTGGGGACTT TCCAGGGAGG CGTGGCCTGG GCGGGACTGG GGAGTGGCGA GCCCTCAGAT 421 CCTGCATATA AGCAGCTGCT TTTTGCCTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA 481 GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT 541 TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC 601 AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACTTGAAAG 661 CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG GCTTGCTGAA GCGCGCACGG 721 CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT TTTGACTAGC GGAGGCTAGA 781 AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG GAGAATTAGA TCGCGATGGG 841 AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA ATTAAAACAT ATAGTATGGG 901 CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT GTTAGAAACA TCAGAAGGCT 961 GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC AGGATCAGAA GAACTTAGAT 1021 CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA AAGGATAGAG ATAAAAGACA 1081 CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA AAGTAAGACC ACCGCACAGC 1141 AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT ATGAGGGACA ATTGGAGAAG 1201 TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA GGAGTAGCAC CCACCAAGGC 1261 AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA ATAGGAGCTT TGTTCCTTGG 1321 GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA ATGACGCTGA CGGTACAGGC 1381 CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT TTGCTGAGGG CTATTGAGGC 1441 GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG CAGCTCCAGG CAAGAATCCT 1501 GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG ATTTGGGGTT GCTCTGGAAA 1561 ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG AGTAATAAAT CTCTGGAACA 1621 GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA ATTAACAATT ACACAAGCTT 1681 AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA AAGAATGAAC AAGAATTATT 1741 GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC ATAACAAATT GGCTGTGGTA 1801 TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT TTAAGAATAG TTTTTGCTGT 1861 ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA TTATCGTTTC AGACCCACCT 1921 CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA GAAGAAGGTG GAGAGAGAGA 1981 CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCTCGACGG TATCGCCTTT AAAAGAAAAG 2041 GGGGGATTGG GGGGTACAGT GCAGGGGAAA GAATAGTAGA CATAATAGCA ACAGACATAC 2101 AAACTAAAGA ATTACAAAAA CAAATTACAA AAATTCAAAA TTTTCGGGTT TATTACAGGG 2161 ACAGCAGAGA TCCAGTTTAT CGATGAGGCC CTTTCGTCTT CACTCGAGGT GCCCGTCAGT 2221 GGGCAGAGCG CACATCGCCC ACAGTCCCCG AGAAGTTGGG GGGAGGGGTC GGCAATTGAA 2281 CCGGTGCCTA GAGAAGGTGG CGCGGGGTAA ACTGGGAAAG TGATGTCGTG TACTGGCTCC 2341 GCCTTTTTCC CGAGGGTGGG GGAGAACCGT ATATAAGTGC AGTAGTCGCC GTGAACGTTC 2401 TTTTTCGCAA CGGGTTTGCC GCCAGAACAC AGGTAAGTGC CGTGTGTGGT TCCCGCGGGC 2461 CTGGCCTCTT TACGGGTTAT GGCCCTTGCG TGCCTTGAAT TACTTCCACC TGGCTGCAGT 2521 ACGTGATTCT TGATCCCGAG CTTCGGGTTG GAAGTGGGTG GGAGAGTTCG AGGCCTTGCG 2581 CTTAAGGAGC CCCTTCGCCT CGTGCTTGAG TTGAGGCCTG GCCTGGGCGC TGGGGCCGCC 2641 GCGTGCGAAT CTGGTGGCAC CTTCGCGCCT GTCTCGCTGC TTTCGATAAG TCTCTAGCCA 2701 TTTAAAATTT TTGATGACCT GCTGCGACGC TTTTTTTCTG GCAAGATAGT CTTGTAAATG 2761 CGGGCCAAGA TCTGCACACT GGTATTTCGG TTTTTGGGGC CGCGGGCGGC GACGGGGCCC 2821 GTGCGTCCCA GCGCACATGT TCGGCGAGGC GGGGCCTGCG AGCGCGGCCA CCGAGAATCG 2881 GACGGGGGTA GTCTCAAGCT GGCCGGCCTG CTCTGGTGCC TGGCCTCGCG CCGCCGTGTA 2941 TCGCCCCGCC CTGGGCGGCA AGGCTGGCCC GGTCGGCACC AGTTGCGTGA GCGGAAAGAT 3001 GGCCGCTTCC CGGCCCTGCT GCAGGGAGCT CAAAATGGAG GACGCGGCGC TCGGGAGAGC 3061 GGGCGGGTGA GTCACCCACA CAAAGGAAAA GGGCCTTTCC GTCCTCAGCC GTCGCTTCAT 3121 GTGACTCCAC GGAGTACCGG GCGCCGTCCA GGCACCTCGA TTAGTTCTCG AGCTTTTGGA 3181 GTACGTCGTC TTTAGGTTGG GGGGAGGGGT TTTATGCGAT GGAGTTTCCC CACACTGAGT 3241 GGGTGGAGAC TGAAGTTAGG CCAGCTTGGC ACTTGATGTA ATTCTCCTTG GAATTTGCCC 3301 TTTTTGAGTT TGGATCTTGG TTCATTCTCA AGCCTCAGAC AGTGGTTCAA AGTTTTTTTC 3361 TTCCATTTCA GGTGTCGTGA ACGCGTGCCA CCatgGAACA AAAACTCATC TCAGAAGAGG 3421 ATCTGATGTG GCGAGCAGGT CGGGCGGCCG TGGCCTGTGA AGTCTGCCAA TCCTTAGTGA 3481 AACACAGTTC TGGAATACAA AGAAACGTAC CGCTCCAAAA ACTCCATCTG GTTTCACGAA 3541 GTATTTATCG TTCACATCAT CCTGCCCTCA AGCTTCAAAG ACCCCAACTA AGGACACCAT 3601 TTCAGCAGTT CTCTTCTCTA ACTCACCTTT CATTACATAA ATTGAAACTT TCTCCAATTA 3661 AATATGGCTA CCAGCCCCGC AGGAACTTTT GGCCAGCAAG GTTAGCTGCA AGACTCTTAA 3721 AACTTCGATA TATCATACTG GGATCTGCTG TTGGAGGTGG CTATACAGCC AAAAAAACCT 3781 TCGATGAATG GAAAGATATG ATACCAGACC TTAGTGACTA TAAGTGGATT GTGCCTGACT 3841 TTATATGGGA AATTGATGAG TATATTGATT TGGAGAAAAT TAGAAAAGCC CTGCCCAGCT 3901 CAGAAGACCT TGCCAGTTTA GCTCCCGACC TGGACAAGAT TACTGAGAGC CTCAGCTTGT 3961 TGAAGGACTT CTTCACTGCA GGTCCCAAAT TGGTTAGTGA AGTCCTAGAA GTTTCTGAGG 4021 CCCTTCTCTT GTTAGGTTCA CCTGGAGAAA CAGCATTTCG AGCAACAGAT CATGGATCTG 4081 AAAGTGACAA GCATTACAGG AAGGGTCTGC TTGGTGAGCT CATTCTCTTA CAACAGCAAA 4141 TTCAAGAGCA CGAAGAGGAA GCACGCAGAG CCGCTGGCCA GTATAGCACG AGCTATGCCC 4201 AACAGAAGCG CAAGGTGTCA GACAAAGAAA AGATTGACCA ACTTCAAGAA GAACTTCTGC 4261 ATACTCAGTT AAAGTATCAG AGGATCTTGG AGCGCCTGGA AAAGGAGAAC AAAGAGCTGC 4321 GGAAGCTGGT GCTGCAGAAG GACGACAAAG GCATCCACCA CAGGAAGCTC AAGAAATCTT 4381 TGATTGATAT GTATTCTGAA GTTCTTGATG TTCTTTCTGA TTATGATGCC AGTTACAATA 4441 CACAAGATCA CCTACCACGG GTTGTTGTGG TTGGAGATCA GAGTGCTGGG AAAACCAGTG 4501 TGCTGGAAAT GATTGCTCAG GCCCGGATCT TCCCGAGAGG GTCCGGCGAG ATGATGACAC 4561 GCTCTCCAGT GAAGGTGACT CTCAGTGAAG GCCCTCACCA TGTGGCCTTG TTTAAAGATA 4621 GCTCTCGGGA ATTTGATCTC ACCAAGGAGG AAGATCTTGC AGCATTAAGA CATGAAATCG 4681 AACTCCGAAT GAGGAAAAAT GTGAAAGAAG GTTGTACTGT TAGCCCCGAG ACCATATCTC 4741 TAAATGTCAA AGGCCCTGGG CTGCAGAGGA TGGTGCTCGT GGACTTGCCT GGTGTCATCA 4801 ACACCGTGAC ATCAGGCATG GCTCCCGACA CAAAGGAAAC TATTTTCAGT ATCAGCAAAG 4861 CTTACATGCA GAATCCTAAC GCCATCATCC TGTGCATCCA AGACGGATCC GTAGATGCTG 4921 AGCGCAGTAT TGTTACAGAC TTGGTCAGTC AAATGGATCC TCATGGAAGA AGAACCATAT 4981 TTGTTTTGAC CAAAGTAGAC CTGGCAGAAA AAAATGTAGC CAGTCCAAGC AGGATACAAC 5041 AGATAATTGA AGGCAAGCTC TTCCCAATGA AAGCTCTGGG TTATTTTGCT GTCGTAACAG 5101 GAAAAGGAAA CAGCTCTGAA AGTATTGAAG CTATAAGAGA GTATGAAGAG GAATTTTTTC 5161 AGAATTCAAA ACTGCTAAAG ACAAGCATGC TAAAGGCACA CCAGGTCACC ACGAGAAATC 5221 TCAGCCTTGC TGTGTCAGAC TGCTTTTGGA AAATGGTTCG AGAGTCAGTT GAACAACAGG 5281 CTGATAGTTT TAAAGCCACG CGCTTTAACC TAGAGACGGA ATGGAAGAAT AACTACCCGC 5341 GCCTGCGAGA GCTCGACAGG AATGAACTCT TTGAAAAAGC TAAAAATGAG ATCCTCGATG 5401 AGGTCATCAG TCTGAGCCAG GTCACGCCAA AGCACTGGGA GGAAATCCTG CAGCAATCCC 5461 TGTGGGAACG AGTGTCAACA CATGTGATTG AGAACATCTA CCTTCCAGCT GCCCAGACCA 5521 TGAATTCGGG AACATTTAAC ACCACAGTAG ACATCAAGCT TAAACAGTGG ACTGACAAGC 5581 AGCTTCCTAA TAAAGCAGTC GAGGTTGCCT GGGAGACTCT ACAAGAGGAA TTTTCCCGCT 5641 TCATGACAGA ACCCAAAGGA AAGGAACACG ACGACATATT TGACAAACTT AAGGAGGCTG 5701 TGAAGGAGGA GAGTATCAAG CGGCACAAGT GGAACGACTT TGCCGAGGAT AGCTTGAGGG 5761 TTATTCAGCA CAATGCTTTG GAAGACCGGT CCATATCAGA TAAGCAACAG TGGGATGCAG 5821 CCATTTACTT CATGGAAGAG GCGCTTCAAG GTCGTCTCAA GGATACTGAA AATGCTATTG 5881 AAAACATGAT TGGGCCAGAC TGGAAAAAGA GGTGGATGTA CTGGAAGAAT CGGACCCAAG 5941 AGCAGTGTGT TCACAACGAA ACCAAGAACG AGTTGGAGAA GATGCTGAAG GTTAATGATG 6001 AGCACCCAGC TTACCTGGCA AGTGATGAGA TTACCACAGT CCGGAAGAAC CTGGAGTCTC 6061 GAGGAGTGGA AGTCGATCCA AGCTTGATTA AGGATACTTG GCATCAAGTT TATAGAAGAC 6121 ATTTCTTAAA AACAGCTCTA AATCATTGTA ACCTTTGTCG CAGAGGTTTT TATTACTACC 6181 AGAGGCATTT TATAGATTCT GAGCTGGAAT GCAATGACGT GGTCCTGTTT TGGCGAATAC 6241 AGCGCATGCT CGCTATCACT GCCAATACAT TAAGGCAGCA GCTTACAAAC ACTGAAGTTA 6301 GGCGACTAGA GAAAAACGTT AAAGAGGTAT TAGAAGATTT TGCAGAAGAC GGTGAGAAGA 6361 AGGTTAAATT GCTCACTGGC AAACGAGTTC AGCTGGCAGA AGATCTCAAG AAAGTTAGAG 6421 AAATTCAAGA AAAGCTTGAT GCTTTCATTG AAGCTCTTCA CCAGGAGAAG TAGTCTAGAG 6481 TCGACGGGCC GCGGTAACAA TTGTTAACTA ACTTAAGCTA GCAACGGTTT CCCTCTAGCG 6541 GGATCAATTC CGCCCCCCCC CCCTAACGTT ACTGGCCGAA GCCGCTTGGA ATAAGGCCGG 6601 TGTGCGTTTG TCTATATGTT ATTTTCCACC ATATTGCCGT CTTTTGGCAA TGTGAGGGCC 6661 CGGAAACCTG GCCCTGTCTT CTTGACGAGC ATTCCTAGGG GTCTTTCCCC TCTCGCCAAA 6721 GGAATGCAAG GTCTGTTGAA TGTCGTGAAG GAAGCAGTTC CTCTGGAAGC TTCTTGAAGA 6781 CAAACAACGT CTGTAGCGAC CCTTTGCAGG CAGCGGAACC CCCCACCTGG CGACAGGTGC 6841 CTCTGCGGCC AAAAGCCACG TGTATAAGAT ACACCTGCAA AGGCGGCACA ACCCCAGTGC 6901 CACGTTGTGA GTTGGATAGT TGTGGAAAGA GTCAAATGGC TCTCCTCAAG CGTATTCAAC 6961 AAGGGGCTGA AGGATGCCCA GAAGGTACCC CATTGTATGG GATCTGATCT GGGGCCTCGG 7021 TGCACATGCT TTACATGTGT TTAGTCGAGG TTAAAAAAAC GTCTAGGCCC CCCGAACCAC 7081 GGGGACGTGG TTTTCCTTTG AAAAACACGA TAATACCATG ACCGAGTACA AGCCCACGGT 7141 GCGCCTCGCC ACCCGCGACG ACGTCCCCAG GGCCGTACGC ACCCTCGCCG CCGCGTTCGC 7201 CGACTACCCC GCCACGCGCC ACACCGTCGA TCCGGACCGC CACATCGAGC GGGTCACCGA 7261 GCTGCAAGAA CTCTTCCTCA CGCGCGTCGG GCTCGACATC GGCAAGGTGT GGGTCGCGGA 7321 CGACGGCGCC GCGGTGGCGG TCTGGACCAC GCCGGAGAGC GTCGAAGCGG GGGCGGTGTT 7381 CGCCGAGATC GGCCCGCGCA TGGCCGAGTT GAGCGGTTCC CGGCTGGCCG CGCAGCAACA 7441 GATGGAAGGC CTCCTGGCGC CGCACCGGCC CAAGGAGCCC GCGTGGTTCC TGGCCACCGT 7501 CGGCGTCTCG CCCGACCACC AGGGCAAGGG TCTGGGCAGC GCCGTCGTGC TCCCCGGAGT 7561 GGAGGCGGCC GAGCGCGCCG GGGTGCCCGC CTTCCTGGAG ACCTCCGCGC CCCGCAACCT 7621 CCCCTTCTAC GAGCGGCTCG GCTTCACCGT CACCGCCGAC GTCGAGGTGC CCGAAGGACC 7681 GCGCACCTGG TGCATGACCC GCAAGCCCGG TGCCTGACCC GGGCGGGGCG CGTCTGGAAC 7741 AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG GTATTCTTAA CTATGTTGCT 7801 CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT ATCATGCTAT TGCTTCCCGT 7861 ATGGCTTTCA TTTTCTCCTC CTTGTATAAA TCCTGGTTGC TGTCTCTTTA TGAGGAGTTG 7921 TGGCCCGTTG TCAGGCAACG TGGCGTGGTG TGCACTGTGT TTGCTGACGC AACCCCCACT 7981 GGTTGGGGCA TTGCCACCAC CTGTCAGCTC CTTTCCGGGA CTTTCGCTTT CCCCCTCCCT 8041 ATTGCCACGG CGGAACTCAT CGCCGCCTGC CTTGCCCGCT GCTGGACAGG GGCTCGGCTG 8101 TTGGGCACTG ACAATTCCGT GGTGTTGTCG GGGAAGCTGA CGTCCTTTCC ATGGCTGCTC 8161 GCCTGTGTTG CCACCTGGAT TCTGCGCGGG ACGTCCTTCT GCTACGTCCC TTCGGCCCTC 8221 AATCCAGCGG ACCTTCCTTC CCGCGGCCTG CTGCCGGCTC TGCGGCCTCT TCCGCGTCTT 8281 CGCCTTCGCC CTCAGACGAG TCGGATCTCC CTTTGGGCCG CCTCCCCGCC TGGAATTAAT 8341 TCTGCAGTCG AGACCTAGAA AAACATGGAG CAATCACAAG TAGCAATACA GCAGCTACCA 8401 ATGCTGATTG TGCCTGGCTA GAAGCACAAG AGGAGGAGGA GGTGGGTTTT CCAGTCACAC 8461 CTCAGGTACC TTTAAGACCA ATGACTTACA AGGCAGCTGT AGATCTTAGC CACTTTTTAA 8521 AAGAAAAGAG GGGACTGGAA GGGCTAATTC ACTCCCAACG AAGACAAGAT ATCCTTGATC 8581 TGTGGATCTA CCACACACAA GGCTACTTCC CTGATTAGCA GAACTACACA CCAGGGCCAG 8641 GGGTCAGATA TCCACTGACC TTTGGATGGT GCTACAAGCT AGTACCAGTT GAGCCAGATA 8701 AGGTAGAAGA GGCCAATAAA GGAGAGAACA CCAGCTTGTT ACACCCTGTG AGCCTGCATG 8761 GGATGGATGA CCCGGAGAGA GAAGTGTTAG AGTGGAGGTT TGACAGCCGC CTAGCATTTC 8821 ATCACGTGGC CCGAGAGCTG CATCCGGAGT ACTTCAAGAA CTGCTGATAT CGAGCTTGCT 8881 ACAAGGGACT TTCCGCTGGG GACTTTCCAG GGAGGCGTGG CCTGGGCGGG ACTGGGGAGT 8941 GGCGAGCCCT CAGATCCTGC ATATAAGCAG CTGCTTTTTG CCTGTACTGG GTCTCTCTGG 9001 TTAGACCAGA TCTGAGCCTG GGAGCTCTCT GGCTAACTAG GGAACCCACT GCTTAAGCCT 9061 CAATAAAGCT TGCCTTGAGT GCTTCAAGTA GTGTGTGCCC GTCTGTTGTG TGACTCTGGT 9121 AACTAGAGAT CCCTCAGACC CTTTTAGTCA GTGTGGAAAA TCTCTAGCAG TAGTAGTTCA 9181 TGTCATCTTA TTATTCAGTA TTTATAACTT GCAAAGAAAT GAATATCAGA GAGTGAGAGG 9241 CCTTGACATT GCTAGCGTTT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA ATCATGGTCA 9301 TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA 9361 AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG 9421 CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC 9481 CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC 9541 TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA 9601 CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA 9661 AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT 9721 GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA 9781 AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG 9841 CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA 9901 CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA 9961 CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG 10021 GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG 10081 TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA 10141 ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC 10201 TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG 10261 ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC 10321 GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC 10381 TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG 10441 TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT 10501 CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG 10561 GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA 10621 GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT 10681 TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA 10741 GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG 10801 TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC 10861 ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG 10921 GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA 10981 TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT 11041 ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC 11101 AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC 11161 TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA 11221 TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA 11281 AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT 11341 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 11401 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGTCGACGGA 11461 TCGGGAGATC AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC 11521 AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT 11581 CAATGTATCT TATCATGTCT GGATCAACTG GATAACTCAA GCTAACCAAA ATCATCCCAA 11641 ACTTCCCACC CCATACCCTA TTACCACTGC CAATTACCTG TGGTTTCATT TACTCTAAAC 11701 CTGTGATTCC TCTGAATTAT TTTCATTTTA AAGAAATTGT ATTTGTTAAA TATGTACTAC 11761 AAACTTAGTA GT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.