pPrdm16-Fluc plasmid

pPrdm16-Fluc plasmid

PVT96637

pPrdm16-Fluc plasmid Information

Plasmid name: pPrdm16(mouse)-Fluc
Alias: -
Gene lenght: 2001bp
Host: Mammalian cells
Use(s):
Promoter detection
Fragment type: Promoter
Species: Mouse
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Fluc
Promoter: Prdm16
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: RVP3
Reverse primer:
Copy number: High
Induction:

pPrdm16-Fluc plasmid Description

pPrdm16-Fluc is a plasmid suitable for promoter detection in mammalian cells.

pPrdm16-Fluc plasmid Sequence

1 ggtaccAGCC TCGGTGCCTG CAAGCTTCAT TAAGGGAACC CCTGTCCTCT GGAGGATAGG 61 AGGGCTGTGA CCCCCTCAAG CTTAGACTTA GTGGGTCTGA GCACTATACA AAGCCATCCT 121 TTCCACTCGG GCACACACCC TGGGTGCGCT CACTCCGTTT GTATTTCACC CAGCTAGGCC 181 TGGAGCTGCC CCAGCAGATG AATGGGGAGA GCAAAAACCC TAGGGCTACT GGGGAGCAGG 241 GGGGCTCTCC TGACTCACCC AGAATGCCTC TCTTATGGAG GCCCAGGGAT ATCCCCCTGC 301 AGTCTGCCTC CTCCACCGTC TGATCCCCAA CTTGATCCTG CCCTGGTGGA GAAGCCTTGG 361 GATGAACTAA CCTGAAAAGG TAAATGCCAA GGTCCAAGTG CAGCTGCCCC AAGTCCGCGT 421 GGCACGCTCC GCGCTCCTGC TCCCACTCCT TCTGCACCTT GTAAGGCTGG GGCTAGCTCA 481 CATCTCTGGT GAATAGTCAC AACAGCCACC ATCCATCTCT GCCCAGGGGC TCTGGTTTTC 541 TCCCTGGCTC TTGGCTCCTC CTTGCCCCAG GCGAGTTACC CATTCTCTCT CTCTTTTCTT 601 CCAAAGCAAG TCTGGCTGTT TTGGGGCAGT AGTGCCAAGC AGTAAATGCT TTTGATGCAA 661 CACCAAGGTT GCAGGTCTGC CTTCCAGCAG CTATCCAGGG TGACCTCTTA AGAGCACTTG 721 TTCAGGGCTG CTGAGAAAGC TCAGAGGTAA AGGTGCTTGA TCCCCGAGGA GCCACAGGAT 781 AGCAGGAAAG AGTTGACTAC CTCAAGTTTT CCTTTAACCT CCACACATAT GCCACTGCAT 841 GCACACACTC ATAGACATGC ACACATAAAT GATGTTGCTT ATTTAAAAGA CCCTGTATTG 901 AGTGGCCTGG GCTAATGAAA TTGACCTTGA AAATGCATGA CACCTGGAGC TGCCCTTTGA 961 CCTCCCACCA CTCTTTGCCT TCTGTGTCCT TTTCCCTACC TTGAATATCC AGCTGCCTCC 1021 GCACTCACTC TAGCCACTGG CCTCACCTTT GGCTTTTCCT CTTCCATCAG ACTTCAGTGA 1081 TGGGTCACAT GAGCCTCACA CCTCCCACCC CACCCCACCA AGTCACCATC CTTCCCCAAG 1141 TGTTACACCA ATACACTGTT GGACACAAGA CAGGTATCTA GACTCTAATC AACAGCTTAG 1201 AAGTGCTTGC AGGTGATTGG GCCTCAAGAC AGAAAGGATC TCCTGACTCA TGGCATCCTC 1261 TGCTCTGGAA GAGGGTCCTC CATGCAGAGC TCTTGGTGCC TGCACCAGCC TAGATACAGA 1321 CAGAGGAAGG CTACTGCACC AGGGAATAAG TGGGTGGGGG AGTGAATGAT GGCAGGGGGT 1381 GAATGAATAG ATGGTAGGTG AAGGGATGGG ATGGGCAGGG CAGTGGAGAA GTGGATGGGT 1441 GGATGGATGG ATGGATGGAT GGATGGATGG ATGGATGGAT GGATGGGTGG AAGGACAGAT 1501 TGAGGATGGA TTTATATATG GATGGGTAGG TGGTGGATGG GAGGATAAAT GGTAAGTGGA 1561 TGGATGGATA GATGAATGGG TGAATAAGTA GATGGATGGA TAGATGGATG GATGGATGGA 1621 TGGATGAATA GGTAGATGGG TGAATGGGTT GTAGATAGAT GGATGGATAG ATGGATGATG 1681 GGTGATTGAT GGATGAATGT ATGGGTAGGT GGATGGATGG GTGGATAGGT GGCTCAATGG 1741 ATAGGATAGG TGGATTGATC AATGGATGGA TGGATGGATG GATGGGTGGG TGGGTGGATG 1801 GATGATGGAT GGATGGGTGG ATATGGATGG ATGGATGGGT GGGTGGGTGG ATGGATGATG 1861 GATGGATGGG TGGATATGGA TGGATGGATG GATGGGTGGG TGGATGGATG GGTGGATGAA 1921 TGGATGGATA TGGATGGATG GATATGGATG GATGGATATG GATGGATGGG TGGATGGATG 1981 GATATGGATG GATGGATGGA TGGATGGctc gagatctgcg atctaagtaa gcttggcatt 2041 ccggtactgt tggtaaagcc accatggaag acgccaaaaa cataaagaaa ggcccggcgc 2101 cattctatcc gctggaagat ggaaccgctg gagagcaact gcataaggct atgaagagat 2161 acgccctggt tcctggaaca attgctttta cagatgcaca tatcgaggtg gacatcactt 2221 acgctgagta cttcgaaatg tccgttcggt tggcagaagc tatgaaacga tatgggctga 2281 atacaaatca cagaatcgtc gtatgcagtg aaaactctct tcaattcttt atgccggtgt 2341 tgggcgcgtt atttatcgga gttgcagttg cgcccgcgaa cgacatttat aatgaacgtg 2401 aattgctcaa cagtatgggc atttcgcagc ctaccgtggt gttcgtttcc aaaaaggggt 2461 tgcaaaaaat tttgaacgtg caaaaaaagc tcccaatcat ccaaaaaatt attatcatgg 2521 attctaaaac ggattaccag ggatttcagt cgatgtacac gttcgtcaca tctcatctac 2581 ctcccggttt taatgaatac gattttgtgc cagagtcctt cgatagggac aagacaattg 2641 cactgatcat gaactcctct ggatctactg gtctgcctaa aggtgtcgct ctgcctcata 2701 gaactgcctg cgtgagattc tcgcatgcca gagatcctat ttttggcaat caaatcattc 2761 cggatactgc gattttaagt gttgttccat tccatcacgg ttttggaatg tttactacac 2821 tcggatattt gatatgtgga tttcgagtcg tcttaatgta tagatttgaa gaagagctgt 2881 ttctgaggag ccttcaggat tacaagattc aaagtgcgct gctggtgcca accctattct 2941 ccttcttcgc caaaagcact ctgattgaca aatacgattt atctaattta cacgaaattg 3001 cttctggtgg cgctcccctc tctaaggaag tcggggaagc ggttgccaag aggttccatc 3061 tgccaggtat caggcaagga tatgggctca ctgagactac atcagctatt ctgattacac 3121 ccgaggggga tgataaaccg ggcgcggtcg gtaaagttgt tccatttttt gaagcgaagg 3181 ttgtggatct ggataccggg aaaacgctgg gcgttaatca aagaggcgaa ctgtgtgtga 3241 gaggtcctat gattatgtcc ggttatgtaa acaatccgga agcgaccaac gccttgattg 3301 acaaggatgg atggctacat tctggagaca tagcttactg ggacgaagac gaacacttct 3361 tcatcgttga ccgcctgaag tctctgatta agtacaaagg ctatcaggtg gctcccgctg 3421 aattggaatc catcttgctc caacacccca acatcttcga cgcaggtgtc gcaggtcttc 3481 ccgacgatga cgccggtgaa cttcccgccg ccgttgttgt tttggagcac ggaaagacga 3541 tgacggaaaa agagatcgtg gattacgtcg ccagtcaagt aacaaccgcg aaaaagttgc 3601 gcggaggagt tgtgtttgtg gacgaagtac cgaaaggtct taccggaaaa ctcgacgcaa 3661 gaaaaatcag agagatcctc ataaaggcca agaagggcgg aaagatcgcc gtgtaattct 3721 agagtcgggg cggccggccg cttcgagcag acatgataag atacattgat gagtttggac 3781 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg 3841 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt 3901 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca 3961 aatgtggtaa aatcgataag gatccgtcga ccgatgccct tgagagcctt caacccagtc 4021 agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt 4081 atcatgcaac tcgtaggaca ggtgccggca gcgctcttcc gcttcctcgc tcactgactc 4141 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 4201 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 4261 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 4321 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 4381 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 4441 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg 4501 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 4561 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 4621 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 4681 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac 4741 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 4801 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 4861 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 4921 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 4981 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 5041 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 5101 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 5161 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 5221 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 5281 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 5341 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 5401 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 5461 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 5521 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 5581 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 5641 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 5701 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 5761 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 5821 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 5881 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 5941 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 6001 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag 6061 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag 6121 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt 6181 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca 6241 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata 6301 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca 6361 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc 6421 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa 6481 caaaatatta acgtttacaa tttcccattc gccattcagg ctgcgcaact gttgggaagg 6541 gcgatcggtg cgggcctctt cgctattacg ccagcccaag ctaccatgat aagtaagtaa 6601 tattaaggta cgggaggtac ttggagcggc cgcaataaaa tatctttatt ttcattacat 6661 ctgtgtgttg gttttttgtg tgaatcgata gtactaacat acgctctcca tcaaaacaaa 6721 acgaaacaaa acaaactagc aaaataggct gtccccagtg caagtgcagg tgccagaaca 6781 tttctctatc gata

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.