pPRDM12-Fluc plasmid

pPRDM12-Fluc plasmid

PVT91829

pPRDM12-Fluc plasmid Information

Plasmid name: pPRDM12(human)-Fluc
Alias: -
Gene lenght: 326bp
Host: Mammalian cells
Use(s):
Promoter detection
Fragment type: Promoter
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Fluc
Promoter: PRDM12
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: Fluc
Reverse primer:
Copy number: High
Induction:

pPRDM12-Fluc plasmid Description

pPRDM12-Fluc is a plasmid suitable for promoter detection in mammalian cells.

pPRDM12-Fluc plasmid Sequence

1 ggtaccaact agggctgcgc gcgagctcgg ggcgactgtg gcggcccggg gagtgcaggg 61 ccggagaggg aagaggacgc gggctctggc cggggaggga gagcggtgca gggctgggcc 121 ggccagccgg ggcagcaaag taaaggtgaa cgccgggctg ggccaggccg gtgaacatag 181 atcaggcctg aggggagcgc ggggcccgaa gccaggcgtc ccctcttgtc tagcctggtc 241 cctggagtcc tagagagcct cgccgcaccc cctccccttc tccgtcccct cctctcctca 301 gagccggctg agcctccctc cctgccctgc gcaagcttgg cattccggta ctgttggtaa 361 agccaccatg gaagacgcca aaaacataaa gaaaggcccg gcgccattct atccgctgga 421 agatggaacc gctggagagc aactgcataa ggctatgaag agatacgccc tggttcctgg 481 aacaattgct tttacagatg cacatatcga ggtggacatc acttacgctg agtacttcga 541 aatgtccgtt cggttggcag aagctatgaa acgatatggg ctgaatacaa atcacagaat 601 cgtcgtatgc agtgaaaact ctcttcaatt ctttatgccg gtgttgggcg cgttatttat 661 cggagttgca gttgcgcccg cgaacgacat ttataatgaa cgtgaattgc tcaacagtat 721 gggcatttcg cagcctaccg tggtgttcgt ttccaaaaag gggttgcaaa aaattttgaa 781 cgtgcaaaaa aagctcccaa tcatccaaaa aattattatc atggattcta aaacggatta 841 ccagggattt cagtcgatgt acacgttcgt cacatctcat ctacctcccg gttttaatga 901 atacgatttt gtgccagagt ccttcgatag ggacaagaca attgcactga tcatgaactc 961 ctctggatct actggtctgc ctaaaggtgt cgctctgcct catagaactg cctgcgtgag 1021 attctcgcat gccagagatc ctatttttgg caatcaaatc attccggata ctgcgatttt 1081 aagtgttgtt ccattccatc acggttttgg aatgtttact acactcggat atttgatatg 1141 tggatttcga gtcgtcttaa tgtatagatt tgaagaagag ctgtttctga ggagccttca 1201 ggattacaag attcaaagtg cgctgctggt gccaacccta ttctccttct tcgccaaaag 1261 cactctgatt gacaaatacg atttatctaa tttacacgaa attgcttctg gtggcgctcc 1321 cctctctaag gaagtcgggg aagcggttgc caagaggttc catctgccag gtatcaggca 1381 aggatatggg ctcactgaga ctacatcagc tattctgatt acacccgagg gggatgataa 1441 accgggcgcg gtcggtaaag ttgttccatt ttttgaagcg aaggttgtgg atctggatac 1501 cgggaaaacg ctgggcgtta atcaaagagg cgaactgtgt gtgagaggtc ctatgattat 1561 gtccggttat gtaaacaatc cggaagcgac caacgccttg attgacaagg atggatggct 1621 acattctgga gacatagctt actgggacga agacgaacac ttcttcatcg ttgaccgcct 1681 gaagtctctg attaagtaca aaggctatca ggtggctccc gctgaattgg aatccatctt 1741 gctccaacac cccaacatct tcgacgcagg tgtcgcaggt cttcccgacg atgacgccgg 1801 tgaacttccc gccgccgttg ttgttttgga gcacggaaag acgatgacgg aaaaagagat 1861 cgtggattac gtcgccagtc aagtaacaac cgcgaaaaag ttgcgcggag gagttgtgtt 1921 tgtggacgaa gtaccgaaag gtcttaccgg aaaactcgac gcaagaaaaa tcagagagat 1981 cctcataaag gccaagaagg gcggaaagat cgccgtgtaa ttctagagtc ggggcggccg 2041 gccgcttcga gcagacatga taagatacat tgatgagttt ggacaaacca caactagaat 2101 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat 2161 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca 2221 gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga 2281 taaggatccg tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg 2341 cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag 2401 gacaggtgcc ggcagcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt 2461 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca 2521 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 2581 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 2641 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 2701 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 2761 gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt 2821 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 2881 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 2941 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 3001 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc 3061 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 3121 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 3181 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 3241 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta 3301 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt 3361 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata 3421 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc 3481 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac 3541 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag 3601 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac 3661 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc 3721 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg 3781 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc 3841 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct 3901 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc 3961 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc 4021 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc 4081 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc 4141 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca 4201 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt 4261 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt 4321 ccgcgcacat ttccccgaaa agtgccacct gacgcgccct gtagcggcgc attaagcgcg 4381 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct 4441 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta 4501 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa 4561 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct 4621 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc 4681 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg 4741 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt 4801 acaatttccc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc 4861 tcttcgctat tacgccagcc caagctacca tgataagtaa gtaatattaa ggtacgggag 4921 gtacttggag cggccgcaat aaaatatctt tattttcatt acatctgtgt gttggttttt 4981 tgtgtgaatc gatagtacta acatacgctc tccatcaaaa caaaacgaaa caaaacaaac 5041 tagcaaaata ggctgtcccc agtgcaagtg caggtgccag aacatttctc tatcgata

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.