pLV2-CCN1-3×FLAG-Puro plasmid

pLV2-CCN1-3×FLAG-Puro plasmid

PVT96627

pLV2-CCN1-3×FLAG-Puro plasmid Information

Plasmid name: pLV2-CCN1(human)-3×FLAG-Puro
Alias: NM_001554.5;CYR61; GIG1; IGFBP10;
Gene lenght: 1143bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-CCN1-3×FLAG-Puro plasmid Description

pLV2-CCN1-3×FLAG-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV2-CCN1-3×FLAG-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcGCCA CCatgagctc ccgcatcgcc agggcgctcg ccttagtcgt 2881 cacccttctc cacttgacca ggctggcgct ctccacctgc cccgctgcct gccactgccc 2941 cctggaggcg cccaagtgcg cgccgggagt cgggctggtc cgggacggct gcggctgctg 3001 taaggtctgc gccaagcagc tcaacgagga ctgcagcaaa acgcagccct gcgaccacac 3061 caaggggctg gaatgcaact tcggcgccag ctccaccgct ctgaagggga tctgcagagc 3121 tcagtcagag ggcagaccct gtgaatataa ctccagaatc taccaaaacg gggaaagttt 3181 ccagcccaac tgtaaacatc agtgcacatg tattgatggc gccgtgggct gcattcctct 3241 gtgtccccaa gaactatctc tccccaactt gggctgtccc aaccctcggc tggtcaaagt 3301 taccgggcag tgctgcgagg agtgggtctg tgacgaggat agtatcaagg accccatgga 3361 ggaccaggac ggcctccttg gcaaggagct gggattcgat gcctccgagg tggagttgac 3421 gagaaacaat gaattgattg cagttggaaa aggcagctca ctgaagcggc tccctgtttt 3481 tggaatggag cctcgcatcc tatacaaccc tttacaaggc cagaaatgta ttgttcaaac 3541 aacttcatgg tcccagtgct caaagacctg tggaactggt atctccacac gagttaccaa 3601 tgacaaccct gagtgccgcc ttgtgaaaga aacccggatt tgtgaggtgc ggccttgtgg 3661 acagccagtg tacagcagcc tgaaaaaggg caagaaatgc agcaagacca agaaatcccc 3721 cgaaccagtc aggtttactt acgctggatg tttgagtgtg aagaaatacc ggcccaagta 3781 ctgcggttcc tgcgtggacg gccgatgctg cacgccccag ctgaccagga ctgtgaagat 3841 gcggttccgc tgcgaagatg gggagacatt ttccaagaac gtcatgatga tccagtcctg 3901 caaatgcaac tacaactgcc cgcatgccaa tgaagcagcg tttcccttct acaggctgtt 3961 caatgacatt cacaaattta gggacggatc cGACTACAAA GACCATGACG GTGATTATAA 4021 AGATCATGAC ATCGACTACA AGGATGACGA TGACAAGtag tctagataat tctaccgggt 4081 aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac 4141 ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca 4201 accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa 4261 gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct 4321 cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg 4381 cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg 4441 gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg 4501 aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc 4561 atctccgggc ctttcgacct gcagcccaag cttaccatga ccgagtacaa gcccacggtg 4621 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc 4681 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag 4741 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac 4801 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc 4861 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag 4921 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc 4981 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg 5041 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc 5101 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg 5161 cgcacctggt gcatgacccg caagcccggt gcctgaccgc gtctggaaca atcaacctct 5221 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct 5281 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat 5341 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt 5401 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat 5461 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc 5521 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga 5581 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc 5641 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga 5701 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc 5761 tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattaatt ctgcagtcga 5821 gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt 5881 gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct 5941 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagagg 6001 ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac 6061 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat 6121 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag 6181 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac 6241 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc 6301 cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt 6361 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc 6421 agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat 6481 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt 6541 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc 6601 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat 6661 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc cttgacattg 6721 ctagcgtttt accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc 6781 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt 6841 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc 6901 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg 6961 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct 7021 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 7081 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 7141 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 7201 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 7261 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 7321 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 7381 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 7441 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 7501 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 7561 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg 7621 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 7681 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 7741 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 7801 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 7861 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 7921 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 7981 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 8041 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 8101 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 8161 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 8221 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg 8281 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 8341 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 8401 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 8461 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 8521 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa 8581 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 8641 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 8701 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 8761 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 8821 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 8881 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc 8941 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca 9001 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct 9061 tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc 9121 ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc 9181 tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta 9241 gtttttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.