PiggyBac-CMV-ASIC1-Puro plasmi

PiggyBac-CMV-ASIC1-Puro plasmid

PVT94487

PiggyBac-CMV-ASIC1-Puro plasmid Information

Plasmid name: PiggyBac-CMV-ASIC1(human)-Puro
Alias: NM_020039.4;ACCN2; ASIC; BNaC2
Gene lenght: 1725bp
Host: Mammalian cells
Use(s): Protein Expression,Transposition
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

PiggyBac-CMV-ASIC1-Puro plasmid Description

PiggyBac-CMV-ASIC1-Puro is a plasmid suitable for protein expression and transposition in mammalian cells.

PiggyBac-CMV-ASIC1-Puro plasmid Sequence

1 ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA 61 CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA 121 AGTGCCACCT AAATTGTAAG CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA 181 ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA 241 TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC 301 GTGGACTCCA ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA 361 CCATCACCCT AATCAAGTTT TTTGGGGTCG AGGTGCCGTA AAGCACTAAA TCGGAACCCT 421 AAAGGGAGCC CCCGATTTAG AGCTTGACGG GGAAAGCCGG CGAACGTGGC GAGAAAGGAA 481 GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT CACGCTGCGC 541 GTAACCACCA CACCCGCCGC GCTTAATGCG CCGCTACAGG GCGCGTCCCA TTCGCCATTC 601 AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG 661 GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA 721 CGACGTTGTA AAACGACGGC CAGTGAGCGC GCCTCGTTCA TTCACGTTTT TGAACCCGTG 781 GAGGACGGGC AGACTCGCGG TGCAAATGTG TTTTACAGCG TGATGGAGCA GATGAAGATG 841 CTCGACACGC TGCAGAACAC GCAGCTAGAT TAACCCTAGA AAGATAATCA TATTGTGACG 901 TACGTTAAAG ATAATCATGC GTAAAATTGA CGCATGTGTT TTATCGGTCT GTATATCGAG 961 GTTTATTTAT TAATTTGAAT AGATATTAAG TTTTATTATA TTTACACTTA CATACTAATA 1021 ATAAATTCAA CAAACAATTT ATTTATGTTT ATTTATTTAT TAAAAAAAAA CAAAAACTCA 1081 AAATTTCTTC TATAAAGTAA CAAAACTTTT ATGAGGGACA GCCCCCCCCC AAAGCCCCCA 1141 GGGATGTAAT TACGTCCCTC CCCCGCTAGG GGGCAGCAGC GAGCCGCCCG GGGCTCCGCT 1201 CCGGTCCGGC GCTCCCCCCG CATCCCCGAG CCGGCAGCGT GCGGGGACAG CCCGGGCACG 1261 GGGAAGGTGG CACGGGATCG CTTTCCTCTG AACGCTTCTC GCTGCTCTTT GAGCCTGCAG 1321 ACACCTGGGG GGATACGGGG AAAAGGCCTC CAAGGCCTAC TAGTATTATG CCCAGTACAT 1381 GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT 1441 GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT 1501 TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA 1561 CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG 1621 GTGGGAGGTC TATATAAGCA GAGCTCGTTT AGTGAACCGT CAGATCGCCT GGAGACGCCA 1681 TCCACGCTGT TTTGACCTCC ATAGAAGATT CTAGAGCTAG CGAATTCGCC ACCatggaac 1741 tgaaggccga ggaggaggag gtgggtggcg tccagccggt gagcatccag gccttcgcca 1801 gcagctccac actgcacggc ctggcccaca tcttctccta cgagcggctg tctctgaagc 1861 gggcactgtg ggccctgtgc ttcctgggct ccctggctgt gctgctgtgt gtgtgcacgg 1921 agcgtgtgca gtactacttc cactaccacc atgtcaccaa gctcgacgag gtggctgcct 1981 ctcagcttac cttccctgct gtcacgctgt gcaacctcaa cgagttccgc tttagccaag 2041 tctccaagaa tgacctgtat catgctgggg agctgctggc cctgctcaac aacaggtatg 2101 agataccaga cacacagatg gcagatgaaa agcagctgga gatactgcag gacaaagcca 2161 acttccgcag cttcaaaccc aaacccttca acatgcgtga gttctacgac cgagctgggc 2221 acgacattcg agacatgctg ctctcctgcc acttccgggg ggaggtctgc agcgctgaag 2281 acttcaaggt ggtcttcaca cgctatggaa agtgctacac gttcaactcg ggccgagatg 2341 ggcggccgcg gctgaagacc atgaagggtg ggacgggcaa tgggctggaa atcatgctgg 2401 acatccagca ggacgagtac ctgcctgtgt ggggggagac tgacgagacg tccttcgaag 2461 caggcatcaa agtgcagatc catagtcagg atgaacctcc tttcatcgac cagctgggct 2521 ttggcgtggc cccaggcttc cagacctttg tggcctgcca ggagcagcgg ctcatctacc 2581 tgcccccacc ctggggcacc tgcaaagctg ttaccatgga ctcggatttg gatttcttcg 2641 actcctacag catcactgcc tgccgcatcg actgtgagac gcgctacctg gtggagaact 2701 gcaactgccg catggtgcac atgccagggg atgccccata ctgtactcca gagcagtaca 2761 aggagtgtgc agatcctgct ctggacttcc tggtggagaa ggaccaggag tactgcgtgt 2821 gtgaaatgcc ttgcaacctg acccgctatg gcaaagagct gtccatggtc aagatcccca 2881 gcaaagcctc agccaagtac ctggccaaga agttcaacaa atctgagcaa tacatagggg 2941 agaacatcct ggtgctggac attttctttg aagtcctcaa ctatgagacc attgaacaga 3001 agaaggccta tgagattgca gggctcctgg gtgagctgct gatgacacct gtccccttct 3061 catgccatgg gcatggcgtg gctccctatc atccaaaagc agggtgctca cttctgtccc 3121 atgagggtcc tccaccccag aggcccttcc ccaaaccctg ttgtcttggt gacatcgggg 3181 gccagatggg gctgttcatc ggggccagca tcctcacggt gctggagctc tttgactacg 3241 cctacgaggt cattaagcac aagctgtgcc gacgaggaaa atgccagaag gaggccaaaa 3301 ggagcagtgc ggacaagggc gtggccctca gcctggacga cgtcaaaaga cacaacccgt 3361 gcgagagcct tcggggccac cctgccggga tgacatacgc tgccaacatc ctacctcacc 3421 atccggcccg aggcacgttc gaggacttta cctgctgaGG ATCCGCGGCC GCGAAGGATC 3481 TGCGATCGCT CCGGTGCCCG TCAGTGGGCA GAGCGCACAT CGCCCACAGT CCCCGAGAAG 3541 TTGGGGGGAG GGGTCGGCAA TTGAACGGGT GCCTAGAGAA GGTGGCGCGG GGTAAACTGG 3601 GAAAGTGATG TCGTGTACTG GCTCCGCCTT TTTCCCGAGG GTGGGGGAGA ACCGTATATA 3661 AGTGCAGTAG TCGCCGTGAA CGTTCTTTTT CGCAACGGGT TTGCCGCCAG AACACAGCTG 3721 AAGCTTCGAG GGGCTCGCAT CTCTCCTTCA CGCGCCCGCC GCCCTACCTG AGGCCGCCAT 3781 CCACGCCGGT TGAGTCGCGT TCTGCCGCCT CCCGCCTGTG GTGCCTCCTG AACTGCGTCC 3841 GCCGTCTAGG TAAGTTTAAA GCTCAGGTCG AGACCGGGCC TTTGTCCGGC GCTCCCTTGG 3901 AGCCTACCTA GACTCAGCCG GCTCTCCACG CTTTGCCTGA CCCTGCTTGC TCAACTCTAC 3961 GTCTTTGTTT CGTTTTCTGT TCTGCGCCGT TACAGATCCA AGCTGTGACC GGCGCCTACG 4021 CTAGACGCCA CCATGACCGA GTACAAGCCC ACGGTGCGCC TCGCCACCCG CGACGACGTC 4081 CCCAGGGCCG TACGCACCCT CGCCGCCGCG TTCGCCGACT ACCCCGCCAC GCGCCACACC 4141 GTCGATCCGG ACCGCCACAT CGAGCGGGTC ACCGAGCTGC AAGAACTCTT CCTCACGCGC 4201 GTCGGGCTCG ACATCGGCAA GGTGTGGGTC GCGGACGACG GCGCCGCGGT GGCGGTCTGG 4261 ACCACGCCGG AGAGCGTCGA AGCGGGGGCG GTGTTCGCCG AGATCGGCCC GCGCATGGCC 4321 GAGTTGAGCG GTTCCCGGCT GGCCGCGCAG CAACAGATGG AAGGCCTCCT GGCGCCGCAC 4381 CGGCCCAAGG AGCCCGCGTG GTTCCTGGCC ACCGTCGGCG TCTCGCCCGA CCACCAGGGC 4441 AAGGGTCTGG GCAGCGCCGT CGTGCTCCCC GGAGTGGAGG CGGCCGAGCG CGCCGGGGTG 4501 CCCGCCTTCC TGGAGACCTC CGCGCCCCGC AACCTCCCCT TCTACGAGCG GCTCGGCTTC 4561 ACCGTCACCG CCGACGTCGA GGTGCCCGAA GGACCGCGCA CCTGGTGCAT GACCCGCAAG 4621 CCCGGTGCCT GAAATCAACC TCTGGATTAC AAAATTTGTG AAAGATTGAC TGGTATTCTT 4681 AACTATGTTG CTCCTTTTAC GCTATGTGGA TACGCTGCTT TAATGCCTTT GTATCAGTTA 4741 ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA 4801 ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT 4861 ATCATGTCTG GAATTGACTC AAATGATGTC AATTAGTCTA TCAGAAGCTA TCTGGTCTCC 4921 CTTCCGGGGG ACAAGACATC CCTGTTTAAT ATTTAAACAG CAGTGTTCCC AAACTGGGTT 4981 CTTATATCCC TTGCTCTGGT CAACCAGGTT GCAGGGTTTC CTGTCCTCAC AGGAACGAAG 5041 TCCCTAAAGA AACAGTGGCA GCCAGGTTTA GCCCCGGAAT TGACTGGATT CCTTTTTTAG 5101 GGCCCATTGG TATGGCTTTT TCCCCGTATC CCCCCAGGTG TCTGCAGGCT CAAAGAGCAG 5161 CGAGAAGCGT TCAGAGGAAA GCGATCCCGT GCCACCTTCC CCGTGCCCGG GCTGTCCCCG 5221 CACGCTGCCG GCTCGGGGAT GCGGGGGGAG CGCCGGACCG GAGCGGAGCC CCGGGCGGCT 5281 CGCTGCTGCC CCCTAGCGGG GGAGGGACGT AATTACATCC CTGGGGGCTT TGGGGGGGGG 5341 CTGTCCCTGA TATCTATAAC AAGAAAATAT ATATATAATA AGTTATCACG TAAGTAGAAC 5401 ATGAAATAAC AATATAATTA TCGTATGAGT TAAATCTTAA AAGTCACGTA AAAGATAATC 5461 ATGCGTCATT TTGACTCACG CGGTCGTTAT AGTTCAAAAT CAGTGACACT TACCGCATTG 5521 ACAAGCACGC CTCACGGGAG CTCCAAGCGG CGACTGAGAT GTCCTAAATG CACAGCGACG 5581 GATTCGCGCT ATTTAGAAAG AGAGAGCAAT ATTTCAAGAA TGCATGCGTC AATTTTACGC 5641 AGACTATCTT TCTAGGGTTA ATCTAGCTGC ATCAGGATCA TATCGTCGGG TCTTTTTTCC 5701 GGCTCAGTCA TCGCCCAAGC TGGCGCTATC TGGGCATCGG GGAGGAAGAA GCCCGTGCCT 5761 TTTCCCGCGA GGTTGAAGCG GCATGGAAAG AGTTTGCCGA GGATGACTGC TGCTGCATTG 5821 ACGTTGAGCG AAAACGCACG TTTACCATGA TGATTCGGGA AGGTGTGGCC ATGCACGCCT 5881 TTAACGGTGA ACTGTTCGTT CAGGCCACCT GGGATACCAG TTCGTCGCGG CTTTTCCGGA 5941 CACAGTTCCG GATGGTCAGC CCGAAGCGCA TCAGCAACCC GAACAATACC GGCGACAGCC 6001 GGAACTGCCG TGCCGGTGTG CAGATTAATG ACAGCGGTGC GGCGCTGGGA TATTACGTCA 6061 GCGAGGACGG GTATCCTGGC TGGATGCCGC AGAAATGGAC ATGGATACCC CGTGAGTTAC 6121 CCGGCGGGCG CGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC 6181 GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA 6241 ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA 6301 CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT 6361 TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG 6421 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 6481 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 6541 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 6601 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 6661 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 6721 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 6781 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 6841 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 6901 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 6961 GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA 7021 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 7081 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 7141 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 7201 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 7261 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT 7321 AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT 7381 CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT 7441 GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG 7501 AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG 7561 TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT 7621 TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC 7681 CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT 7741 CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC 7801 AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA 7861 GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC 7921 GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA 7981 ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA 8041 ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG 8101 AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG 8161 AATACTCAT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.