pMMLV-3×FLAG-Ripk3-BSD plasmid

pMMLV-3×FLAG-Ripk3-BSD plasmid

PVT96610

pMMLV-3×FLAG-Ripk3-BSD plasmid Information

Plasmid name: pMMLV-3×FLAG-Ripk3(mouse)-BSD
Alias: NM_019955.2;2610528K09Rik; Rip3
Gene lenght: 1461bp
Host: Mammalian cells,Retrovirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Blast
Fluorescent labeling:
Promoter: Non
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: pMMLV-F：CCTCAAAGTAGACGGCATCGCAG
Reverse primer:
Copy number: High
Induction:

pMMLV-3×FLAG-Ripk3-BSD plasmid Description

pMMLV-3×FLAG-Ripk3-BSD This is a plasmid suitable for protein expression in mammalian cells and can also be used for retrovirus packaging.

pMMLV-3×FLAG-Ripk3-BSD plasmid Sequence

1 AATGAAAGAC CCCACCTGTA GGTTTGGCAA GCTAGCTTAA GTAACGCCAT TTTGCAAGGC 61 ATGGAAAAAT ACATAACTGA GAATAGAGAA GTTCAGATCA AGGTCAGGAA CAGATGGAAC 121 AGCTGAATAT GGGCCAAACA GGATATCTGT GGTAAGCAGT TCCTGCCCCG GCTCAGGGCC 181 AAGAACAGAT GGAACAGCTG AATATGGGCC AAACAGGATA TCTGTGGTAA GCAGTTCCTG 241 CCCCGGCTCA GGGCCAAGAA CAGATGGTCC CCAGATGCGG TCCAGCCCTC AGCAGTTTCT 301 AGAGAACCAT CAGATGTTTC CAGGGTGCCC CAAGGACCTG AAATGACCCT GTGCCTTATT 361 TGAACTAACC AATCAGTTCG CTTCTCGCTT CTGTTCGCGC GCTTCTGCTC CCCGAGCTCA 421 ATAAAAGAGC CCACAACCCC TCACTCGGCG CGCCAGTCCT CCGATTGACT GAGTCGCCCG 481 GGTACCCGTG TATCCAATAA ACCCTCTTGC AGTTGCATCC GACTTGTGGT CTCGCTGTTC 541 CTTGGGAGGG TCTCCTCTGA GTGATTGACT ACCCGTCAGC GGGGGTCTTT CATTTGGGGG 601 CTCGTCCGGG ATCGGGAGAC CCCTGCCCAG GGACCACCGA CCCACCACCG GGAGGTAAGC 661 TGGCCAGCAA CTTATCTGTG TCTGTCCGAT TGTCTAGTGT CTATGACTGA TTTTATGCGC 721 CTGCGTCGGT ACTAGTTAGC TAACTAGCTC TGTATCTGGC GGACCCGTGG TGGAACTGAC 781 GAGTTCGGAA CACCCGGCCG CAACCCTGGG AGACGTCCCA GGGACTTCGG GGGCCGTTTT 841 TGTGGCCCGA CCTGAGTCCA AAAATCCCGA TCGTTTTGGA CTCTTTGGTG CACCCCCCTT 901 AGAGGAGGGA TATGTGGTTC TGGTAGGAGA CGAGAACCTA AAACAGTTCC CGCCTCCGTC 961 TGAATTTTTG CTTTCGGTTT GGGACCGAAG CCGCGCCGCG CGTCTTGTCT GCTGCAGCAT 1021 CGTTCTGTGT TGTCTCTGTC TGACTGTGTT TCTGTATTTG TCTGAAAATA TGGGCCCGGG 1081 CCAGACTGTT ACCACTCCCT TAAGTTTGAC CTTAGGTCAC TGGAAAGATG TCGAGCGGAT 1141 CGCTCACAAC CAGTCGGTAG ATGTCAAGAA GAGACGTTGG GTTACCTTCT GCTCTGCAGA 1201 ATGGCCAACC TTTAACGTCG GATGGCCGCG AGACGGCACC TTTAACCGAG ACCTCATCAC 1261 CCAGGTTAAG ATCAAGGTCT TTTCACCTGG CCCGCATGGA CACCCAGACC AGGTCCCCTA 1321 CATCGTGACC TGGGAAGCCT TGGCTTTTGA CCCCCCTCCC TGGGTCAAGC CCTTTGTACA 1381 CCCTAAGCCT CCGCCTCCTC TTCCTCCATC CGCCCCGTCT CTCCCCCTTG AACCTCCTCG 1441 TTCGACCCCG CCTCGATCCT CCCTTTATCC AGCCCTCACT CCTTCTCTAG GCGCCCCCAT 1501 ATGGCCATAT GAGATCTTAT ATGGGGCACC CCCGCCCCTT GTAAACTTCC CTGACCCTGA 1561 CATGACAAGA GTTACTAACA GCCCCTCTCT CCAAGCTCAC TTACAGGCTC TCTACTTAGT 1621 CCAGCACGAA GTCTGGAGAC CTCTGGCGGC AGCCTACCAA GAACAACTGG ACCGACCGGT 1681 GGTACCTCAC CCTTACCGAG TCGGCGACAC AGTGTGGGTC CGCCGACACC AGACTAAGAA 1741 CCTAGAACCT CGCTGGAAAG GACCTTACAC AGTCCTGCTG ACCACCCCCA CCGCCCTCAA 1801 AGTAGACGGC ATCGCAGCTT GGATACACGC CGCCCACGTG AAGGCTGCCG ACCCCGGGGG 1861 TGGACCATCC TCTAGACTGC CGGATCTCCC GCCaccatgg actacaaaga ccatgacggt 1921 gattataaag atcatgacat cgactacaag gatgacgatg acaagatgtc ttctgtcaag 1981 ttatggccta ctggtgcgtc agcggttcct ctggtgagcc gtgaagaact gaagaagctg 2041 gagtttgtgg gtaaaggagg gttcggagtc gtgttccggg cacaccacag aacatggaac 2101 catgatgtag cagtcaagat cgtgaactcg aagaagatat cctgggaggt gaaggctatg 2161 gttaatcttc gtaatgagaa cgttctgctc ctgctggggg tcactgagga cctccagtgg 2221 gacttcgtgt ccgggcaggc tctggtgaca agattcatgg agaatggctc cctcgcaggg 2281 ctgctgcaac ccgagtgccc tcggccctgg ccactcctct gtcgcctgct gcaggaagtg 2341 gtgctgggga tgtgctacct acacagcttg aaccctccgc tcctgcaccg ggacctcaag 2401 ccctctaaca ttctgctgga tccagagctc cacgccaagc tagcagattt tggcctgtcc 2461 acgtttcagg gagggtccca gtcagggtca ggatcaggat caggatccag ggactctggg 2521 ggcaccctag cgtacttgga cccagagctg ttatttgatg tcaacctgaa ggcttctaaa 2581 gcgagtgatg tctacagctt tgggatcctc gtgtgggcag tgctggctgg cagagaagct 2641 gagttggtag acaagacttc actaatccgg gaaacagtgt gtgacaggca gagtcgtcct 2701 ccactgacag agctgcctcc aggtagccct gagactcccg gcttggaaaa actgaaggag 2761 ttaatgattc attgctgggg ttcccagtcc gaaaacaggc catccttcca ggactgcgaa 2821 ccaaaaacca atgaagttta caatctggta aaggacaagg tagatgctgc tgtctccgag 2881 gtaaagcatt atctgtctca gcacagaagc agcggcagaa acttgtctgc cagagagcca 2941 agccaaagag gcacagaaat ggattgcccg agggaaacca tggtttctaa aatgctggac 3001 cgcctgcatt tggaggaacc ctccggacca gttcctggaa aatgtcctga gaggcaagca 3061 caggacacat cagttgggcc tgccacacca gcaaggacat cttctgaccc cgtggctggc 3121 actcctcaga ttccacatac tttacccttc agaggcacaa cacctgggcc agtctttact 3181 gagactcccg gtcctcaccc ccaaaggaat cagggagatg gaagacacgg cactccttgg 3241 tatccctgga ccccaccgaa tccaatgaca gggccaccgg ctctcgtctt caacaactgt 3301 tctgaagtgc agattgggaa ctacaactcc ttggtagcac caccaagaac tactgcctca 3361 agttcggcca agtatgacca agcacagttc ggcaggggta ggggctggca gcccttccac 3421 aagtagGGAT CCCAGTGTGG TGGTACGGGA ATTCCTGCAG GCCTCGAGGG CCGGCGCGCC 3481 GCGGCCGCTA CGTAAATTCC GCCCCCCCCC CCCCTCCCCC CCCCCTAACG TTACTGGCCG 3541 AAGCCGCTTG GAATAAGGCC GGTGTGCGTT TGTCTATATG TTATTTTCCA CCATATTGCC 3601 GTCTTTTGGC AATGTGAGGG CCCGGAAACC TGGCCCTGTC TTCTTGACGA GCATTCCTAG 3661 GGGTCTTTCC CCTCTCGCCA AAGGAATGCA AGGTCTGTTG AATGTCGTGA AGGAAGCAGT 3721 TCCTCTGGAA GCTTCTTGAA GACAAACAAC GTCTGTAGCG ACCCTTTGCA GGCAGCGGAA 3781 CCCCCCACCT GGCGACAGGT GCCTCTGCGG CCAAAAGCCA CGTGTATAAG ATACACCTGC 3841 AAAGGCGGCA CAACCCCAGT GCCACGTTGT GAGTTGGATA GTTGTGGAAA GAGTCAAATG 3901 GCTCTCCTCA AGCGTATTCA ACAAGGGGCT GAAGGATGCC CAGAAGGTAC CCCATTGTAT 3961 GGGATCTGAT CTGGGGCCTC GGTGCACATG CTTTACATGT GTTTAGTCGA GGTTAAAAAA 4021 ACGTCTAGGC CCCCCGAACC ACGGGGACGT GGTTTTCCTT TGAAAAACAC GATGATAATA 4081 TGGCCACAAC CATGGCCAAG CCTTTGTCTC AAGAAGAATC CACCCTCATT GAAAGAGCAA 4141 CGGCTACAAT CAACAGCATC CCCATCTCTG AAGACTACAG CGTCGCCAGC GCAGCTCTCT 4201 CTAGCGACGG CCGCATCTTC ACTGGTGTCA ATGTATATCA TTTTACTGGG GGACCTTGTG 4261 CAGAACTCGT GGTGCTGGGC ACTGCTGCTG CTGCGGCAGC TGGCAACCTG ACTTGTATCG 4321 TCGCGATCGG AAATGAGAAC AGGGGCATCT TGAGCCCCTG CGGACGGTGC CGACAGGTGC 4381 TTCTCGATCT GCATCCTGGG ATCAAAGCCA TAGTGAAGGA CAGTGATGGA CAGCCGACGG 4441 CAGTTGGGAT TCGTGAATTG CTGCCCTCTG GTTATGTGTG GGAGGGCTAA CGCCCGCCCC 4501 ACGACCCGCA GCGCCCGACC GAAAGGAGCG CACGACCCCA TGGCTCGACG ATAAAATAAA 4561 AGATTTTATT TAGTCTCCAG AAAAAGGGGG GAATGAAAGA CCCCACCTGT AGGTTTGGCA 4621 AGCTAGCTTA AGTAACGCCA TTTTGCAAGG CATGGAAAAA TACATAACTG AGAATAGAGA 4681 AGTTCAGATC AAGGTCAGGA ACAGATGGAA CAGCTGAATA TGGGCCAAAC AGGATATCTG 4741 TGGTAAGCAG TTCCTGCCCC GGCTCAGGGC CAAGAACAGA TGGAACAGCT GAATATGGGC 4801 CAAACAGGAT ATCTGTGGTA AGCAGTTCCT GCCCCGGCTC AGGGCCAAGA ACAGATGGTC 4861 CCCAGATGCG GTCCAGCCCT CAGCAGTTTC TAGAGAACCA TCAGATGTTT CCAGGGTGCC 4921 CCAAGGACCT GAAATGACCC TGTGCCTTAT TTGAACTAAC CAATCAGTTC GCTTCTCGCT 4981 TCTGTTCGCG CGCTTCTGCT CCCCGAGCTC AATAAAAGAG CCCACAACCC CTCACTCGGG 5041 GCGCCAGTCC TCCGATTGAC TGAGTCGCCC GGGTACCCGT GTATCCAATA AACCCTCTTG 5101 CAGTTGCATC CGACTTGTGG TCTCGCTGTT CCTTGGGAGG GTCTCCTCTG AGTGATTGAC 5161 TACCCGTCAG CGGGGGTCTT TCACATGCAG CATGTATCAA AATTAATTTG GTTTTTTTTC 5221 TTAAGTATTT ACATTAAATG GCCATAGTTG CATTAATGAA TCGGCCAACG CGCGGGGAGA 5281 GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC 5341 GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA 5401 TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT 5461 AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA 5521 AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT 5581 CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG 5641 TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC 5701 AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC 5761 GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA 5821 TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT 5881 ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC 5941 TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA 6001 CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA 6061 AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA 6121 AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT 6181 TTGCGGCCGG CCGCAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC 6241 AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG 6301 CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG 6361 CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC 6421 CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA 6481 TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG 6541 TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT 6601 CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA 6661 GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG 6721 TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA 6781 CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT 6841 GCCCGGCGTC AACACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA 6901 TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT 6961 CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT 7021 CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA 7081 AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT 7141 GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC 7201 GCACATTTCC CCGAAAAGTG CCACCTGACG TCTAAGAAAC CATTATTATC ATGACATTAA 7261 CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGTCTTCA CTCGAGGAGC TTGGCCCATT 7321 GCATACGTTG TATCCATATC ATAATATGTA CATTTATATT GGCTCATGTC CAACATTACC 7381 GCCATGTTGA CATTGATTAT TGACTAGTTA TTAATAGTAA TCAATTACGG GGTCATTAGT 7441 TCATAGCCCA TATATGGAGT TCCGCGTTAC ATAACTTACG GTAAATGGCC CGCCTGGCTG 7501 ACCGCCCAAC GACCCCCGCC CATTGACGTC AATAATGACG TATGTTCCCA TAGTAACGCC 7561 AATAGGGACT TTCCATTGAC GTCAATGGGT GGAGTATTTA CGGTAAACTG CCCACTTGGC 7621 AGTACATCAA GTGTATCATA TGCCAAGTAC GCCCCCTATT GACGTCAATG ACGGTAAATG 7681 GCCCGCCTGG CATTATGCCC AGTACATGAC CTTATGGGAC TTTCCTACTT GGCAGTACAT 7741 CTACGTATTA GTCATCGCTA TTACCATGCT AGAGAACCAT CAGATGTTTC CAGGGTGCCC 7801 CAAGGACCTG AAATGACCCT GTGCCTTATT TGAACTAACC AATCAGTTCG CTTCTCGCTT 7861 CTGTTCGCGC GCTTCTGCTC CCCGAGCTCA ATAAAAGAGC CCACAACCCC TCACTCGGCG 7921 CGCCAGTCCT CCGATTGACT GAGTCGCCCG GGTACCCGTG TATCCAATAA ACCCTCTTGC 7981 AGTTGCATCC GACTTGTGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.