pMMLV-Ripk3-mCherry-BSD plasmi

pMMLV-Ripk3-mCherry-BSD plasmid

PVT96317

pMMLV-Ripk3-mCherry-BSD plasmid Information

Plasmid name: pMMLV-Ripk3(mouse)-mCherry-BSD
Alias: NM_019955.2;2610528K09Rik; Rip3
Gene lenght: 1458bp
Host: Mammalian cells,Retrovirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Blast
Fluorescent labeling: Red
Promoter: Non
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: IRES-F1
Reverse primer:
Copy number: High
Induction:

pMMLV-Ripk3-mCherry-BSD plasmid Description

pMMLV-Ripk3-mCherry-BSD This is a plasmid suitable for protein expression in mammalian cells and can also be used for retrovirus packaging.

pMMLV-Ripk3-mCherry-BSD plasmid Sequence

1 AATGAAAGAC CCCACCTGTA GGTTTGGCAA GCTAGCTTAA GTAACGCCAT TTTGCAAGGC 61 ATGGAAAAAT ACATAACTGA GAATAGAGAA GTTCAGATCA AGGTCAGGAA CAGATGGAAC 121 AGCTGAATAT GGGCCAAACA GGATATCTGT GGTAAGCAGT TCCTGCCCCG GCTCAGGGCC 181 AAGAACAGAT GGAACAGCTG AATATGGGCC AAACAGGATA TCTGTGGTAA GCAGTTCCTG 241 CCCCGGCTCA GGGCCAAGAA CAGATGGTCC CCAGATGCGG TCCAGCCCTC AGCAGTTTCT 301 AGAGAACCAT CAGATGTTTC CAGGGTGCCC CAAGGACCTG AAATGACCCT GTGCCTTATT 361 TGAACTAACC AATCAGTTCG CTTCTCGCTT CTGTTCGCGC GCTTCTGCTC CCCGAGCTCA 421 ATAAAAGAGC CCACAACCCC TCACTCGGCG CGCCAGTCCT CCGATTGACT GAGTCGCCCG 481 GGTACCCGTG TATCCAATAA ACCCTCTTGC AGTTGCATCC GACTTGTGGT CTCGCTGTTC 541 CTTGGGAGGG TCTCCTCTGA GTGATTGACT ACCCGTCAGC GGGGGTCTTT CATTTGGGGG 601 CTCGTCCGGG ATCGGGAGAC CCCTGCCCAG GGACCACCGA CCCACCACCG GGAGGTAAGC 661 TGGCCAGCAA CTTATCTGTG TCTGTCCGAT TGTCTAGTGT CTATGACTGA TTTTATGCGC 721 CTGCGTCGGT ACTAGTTAGC TAACTAGCTC TGTATCTGGC GGACCCGTGG TGGAACTGAC 781 GAGTTCGGAA CACCCGGCCG CAACCCTGGG AGACGTCCCA GGGACTTCGG GGGCCGTTTT 841 TGTGGCCCGA CCTGAGTCCA AAAATCCCGA TCGTTTTGGA CTCTTTGGTG CACCCCCCTT 901 AGAGGAGGGA TATGTGGTTC TGGTAGGAGA CGAGAACCTA AAACAGTTCC CGCCTCCGTC 961 TGAATTTTTG CTTTCGGTTT GGGACCGAAG CCGCGCCGCG CGTCTTGTCT GCTGCAGCAT 1021 CGTTCTGTGT TGTCTCTGTC TGACTGTGTT TCTGTATTTG TCTGAAAATA TGGGCCCGGG 1081 CCAGACTGTT ACCACTCCCT TAAGTTTGAC CTTAGGTCAC TGGAAAGATG TCGAGCGGAT 1141 CGCTCACAAC CAGTCGGTAG ATGTCAAGAA GAGACGTTGG GTTACCTTCT GCTCTGCAGA 1201 ATGGCCAACC TTTAACGTCG GATGGCCGCG AGACGGCACC TTTAACCGAG ACCTCATCAC 1261 CCAGGTTAAG ATCAAGGTCT TTTCACCTGG CCCGCATGGA CACCCAGACC AGGTCCCCTA 1321 CATCGTGACC TGGGAAGCCT TGGCTTTTGA CCCCCCTCCC TGGGTCAAGC CCTTTGTACA 1381 CCCTAAGCCT CCGCCTCCTC TTCCTCCATC CGCCCCGTCT CTCCCCCTTG AACCTCCTCG 1441 TTCGACCCCG CCTCGATCCT CCCTTTATCC AGCCCTCACT CCTTCTCTAG GCGCCCCCAT 1501 ATGGCCATAT GAGATCTTAT ATGGGGCACC CCCGCCCCTT GTAAACTTCC CTGACCCTGA 1561 CATGACAAGA GTTACTAACA GCCCCTCTCT CCAAGCTCAC TTACAGGCTC TCTACTTAGT 1621 CCAGCACGAA GTCTGGAGAC CTCTGGCGGC AGCCTACCAA GAACAACTGG ACCGACCGGT 1681 GGTACCTCAC CCTTACCGAG TCGGCGACAC AGTGTGGGTC CGCCGACACC AGACTAAGAA 1741 CCTAGAACCT CGCTGGAAAG GACCTTACAC AGTCCTGCTG ACCACCCCCA CCGCCCTCAA 1801 AGTAGACGGC ATCGCAGCTT GGATACACGC CGCCCACGTG AAGGCTGCCG ACCCCGGGGG 1861 TGGACCATCC TCTAGACTGC CGGATCTCCC GCCGCCACCa tgtcttctgt caagttatgg 1921 cctactggtg cgtcagcggt tcctctggtg agccgtgaag aactgaagaa gctggagttt 1981 gtgggtaaag gagggttcgg agtcgtgttc cgggcacacc acagaacatg gaaccatgat 2041 gtagcagtca agatcgtgaa ctcgaagaag atatcctggg aggtgaaggc tatggttaat 2101 cttcgtaatg agaacgttct gctcctgctg ggggtcactg aggacctcca gtgggacttc 2161 gtgtccgggc aggctctggt gacaagattc atggagaatg gctccctcgc agggctgctg 2221 caacccgagt gccctcggcc ctggccactc ctctgtcgcc tgctgcagga agtggtgctg 2281 gggatgtgct acctacacag cttgaaccct ccgctcctgc accgggacct caagccctct 2341 aacattctgc tggatccaga gctccacgcc aagctagcag attttggcct gtccacgttt 2401 cagggagggt cccagtcagg gtcaggatca ggatcaggat ccagggactc tgggggcacc 2461 ctagcgtact tggacccaga gctgttattt gatgtcaacc tgaaggcttc taaagcgagt 2521 gatgtctaca gctttgggat cctcgtgtgg gcagtgctgg ctggcagaga agctgagttg 2581 gtagacaaga cttcactaat ccgggaaaca gtgtgtgaca ggcagagtcg tcctccactg 2641 acagagctgc ctccaggtag ccctgagact cccggcttgg aaaaactgaa ggagttaatg 2701 attcattgct ggggttccca gtccgaaaac aggccatcct tccaggactg cgaaccaaaa 2761 accaatgaag tttacaatct ggtaaaggac aaggtagatg ctgctgtctc cgaggtaaag 2821 cattatctgt ctcagcacag aagcagcggc agaaacttgt ctgccagaga gccaagccaa 2881 agaggcacag aaatggattg cccgagggaa accatggttt ctaaaatgct ggaccgcctg 2941 catttggagg aaccctccgg accagttcct ggaaaatgtc ctgagaggca agcacaggac 3001 acatcagttg ggcctgccac accagcaagg acatcttctg accccgtggc tggcactcct 3061 cagattccac atactttacc cttcagaggc acaacacctg ggccagtctt tactgagact 3121 cccggtcctc acccccaaag gaatcaggga gatggaagac acggcactcc ttggtatccc 3181 tggaccccac cgaatccaat gacagggcca ccggctctcg tcttcaacaa ctgttctgaa 3241 gtgcagattg ggaactacaa ctccttggta gcaccaccaa gaactactgc ctcaagttcg 3301 gccaagtatg accaagcaca gttcggcagg ggtaggggct ggcagccctt ccacaagggt 3361 ggaggcggat caggaggcgg tggatctatg gtgagcaagg gcgaggagga taacatggcc 3421 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag 3481 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg 3541 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg 3601 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc 3661 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc 3721 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc 3781 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc 3841 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag 3901 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc 3961 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag 4021 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg 4081 gacgagctgt acaagtagGG ATCCCAGTGT GGTGGTACGG GAATTCCTGC AGGCCTCGAG 4141 GGCCGGCGCG CCGCGGCCGC TACGTAAATT CCGCCCCCCC CCCCCCTCCC CCCCCCCTAA 4201 CGTTACTGGC CGAAGCCGCT TGGAATAAGG CCGGTGTGCG TTTGTCTATA TGTTATTTTC 4261 CACCATATTG CCGTCTTTTG GCAATGTGAG GGCCCGGAAA CCTGGCCCTG TCTTCTTGAC 4321 GAGCATTCCT AGGGGTCTTT CCCCTCTCGC CAAAGGAATG CAAGGTCTGT TGAATGTCGT 4381 GAAGGAAGCA GTTCCTCTGG AAGCTTCTTG AAGACAAACA ACGTCTGTAG CGACCCTTTG 4441 CAGGCAGCGG AACCCCCCAC CTGGCGACAG GTGCCTCTGC GGCCAAAAGC CACGTGTATA 4501 AGATACACCT GCAAAGGCGG CACAACCCCA GTGCCACGTT GTGAGTTGGA TAGTTGTGGA 4561 AAGAGTCAAA TGGCTCTCCT CAAGCGTATT CAACAAGGGG CTGAAGGATG CCCAGAAGGT 4621 ACCCCATTGT ATGGGATCTG ATCTGGGGCC TCGGTGCACA TGCTTTACAT GTGTTTAGTC 4681 GAGGTTAAAA AAACGTCTAG GCCCCCCGAA CCACGGGGAC GTGGTTTTCC TTTGAAAAAC 4741 ACGATGATAA TATGGCCACA ACCATGGCCA AGCCTTTGTC TCAAGAAGAA TCCACCCTCA 4801 TTGAAAGAGC AACGGCTACA ATCAACAGCA TCCCCATCTC TGAAGACTAC AGCGTCGCCA 4861 GCGCAGCTCT CTCTAGCGAC GGCCGCATCT TCACTGGTGT CAATGTATAT CATTTTACTG 4921 GGGGACCTTG TGCAGAACTC GTGGTGCTGG GCACTGCTGC TGCTGCGGCA GCTGGCAACC 4981 TGACTTGTAT CGTCGCGATC GGAAATGAGA ACAGGGGCAT CTTGAGCCCC TGCGGACGGT 5041 GCCGACAGGT GCTTCTCGAT CTGCATCCTG GGATCAAAGC CATAGTGAAG GACAGTGATG 5101 GACAGCCGAC GGCAGTTGGG ATTCGTGAAT TGCTGCCCTC TGGTTATGTG TGGGAGGGCT 5161 AACGCCCGCC CCACGACCCG CAGCGCCCGA CCGAAAGGAG CGCACGACCC CATGGCTCGA 5221 CGATAAAATA AAAGATTTTA TTTAGTCTCC AGAAAAAGGG GGGAATGAAA GACCCCACCT 5281 GTAGGTTTGG CAAGCTAGCT TAAGTAACGC CATTTTGCAA GGCATGGAAA AATACATAAC 5341 TGAGAATAGA GAAGTTCAGA TCAAGGTCAG GAACAGATGG AACAGCTGAA TATGGGCCAA 5401 ACAGGATATC TGTGGTAAGC AGTTCCTGCC CCGGCTCAGG GCCAAGAACA GATGGAACAG 5461 CTGAATATGG GCCAAACAGG ATATCTGTGG TAAGCAGTTC CTGCCCCGGC TCAGGGCCAA 5521 GAACAGATGG TCCCCAGATG CGGTCCAGCC CTCAGCAGTT TCTAGAGAAC CATCAGATGT 5581 TTCCAGGGTG CCCCAAGGAC CTGAAATGAC CCTGTGCCTT ATTTGAACTA ACCAATCAGT 5641 TCGCTTCTCG CTTCTGTTCG CGCGCTTCTG CTCCCCGAGC TCAATAAAAG AGCCCACAAC 5701 CCCTCACTCG GGGCGCCAGT CCTCCGATTG ACTGAGTCGC CCGGGTACCC GTGTATCCAA 5761 TAAACCCTCT TGCAGTTGCA TCCGACTTGT GGTCTCGCTG TTCCTTGGGA GGGTCTCCTC 5821 TGAGTGATTG ACTACCCGTC AGCGGGGGTC TTTCACATGC AGCATGTATC AAAATTAATT 5881 TGGTTTTTTT TCTTAAGTAT TTACATTAAA TGGCCATAGT TGCATTAATG AATCGGCCAA 5941 CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG 6001 CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG 6061 TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG 6121 GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC 6181 GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA 6241 TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT 6301 ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC 6361 TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC 6421 CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA 6481 AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT 6541 GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA 6601 GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT 6661 TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT 6721 ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT 6781 CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC 6841 ACCTAGATCC TTTTGCGGCC GGCCGCAAAT CAATCTAAAG TATATATGAG TAAACTTGGT 6901 CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT 6961 CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT 7021 CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG 7081 CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT 7141 CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT 7201 TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG 7261 CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA 7321 AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT 7381 TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT 7441 GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC 7501 CGAGTTGCTC TTGCCCGGCG TCAACACGGG ATAATACCGC GCCACATAGC AGAACTTTAA 7561 AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT 7621 TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT 7681 TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA 7741 GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT 7801 ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA 7861 TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGTCTAAGAA ACCATTATTA 7921 TCATGACATT AACCTATAAA AATAGGCGTA TCACGAGGCC CTTTCGTCTT CACTCGAGGA 7981 GCTTGGCCCA TTGCATACGT TGTATCCATA TCATAATATG TACATTTATA TTGGCTCATG 8041 TCCAACATTA CCGCCATGTT GACATTGATT ATTGACTAGT TATTAATAGT AATCAATTAC 8101 GGGGTCATTA GTTCATAGCC CATATATGGA GTTCCGCGTT ACATAACTTA CGGTAAATGG 8161 CCCGCCTGGC TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAATGA CGTATGTTCC 8221 CATAGTAACG CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC 8281 TGCCCACTTG GCAGTACATC AAGTGTATCA TATGCCAAGT ACGCCCCCTA TTGACGTCAA 8341 TGACGGTAAA TGGCCCGCCT GGCATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC 8401 TTGGCAGTAC ATCTACGTAT TAGTCATCGC TATTACCATG CTAGAGAACC ATCAGATGTT 8461 TCCAGGGTGC CCCAAGGACC TGAAATGACC CTGTGCCTTA TTTGAACTAA CCAATCAGTT 8521 CGCTTCTCGC TTCTGTTCGC GCGCTTCTGC TCCCCGAGCT CAATAAAAGA GCCCACAACC 8581 CCTCACTCGG CGCGCCAGTC CTCCGATTGA CTGAGTCGCC CGGGTACCCG TGTATCCAAT 8641 AAACCCTCTT GCAGTTGCAT CCGACTTGTG G

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.