pLV3-CMV-TNFSF9 plasmid

pLV3-CMV-TNFSF9 plasmid

PVT96597

pLV3-CMV-TNFSF9 plasmid Information

Plasmid name: pLV3-CMV-TNFSF9(human)
Alias: NM_003811.4;4-1BB-L; CD137L; TNLG5A
Gene lenght: 765bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-TNFSF9 plasmid Description

pLV3-CMV-TNFSF9 It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-TNFSF9 plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga 1981 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt 2041 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt 2101 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca 2161 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt 2221 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt 2281 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca 2341 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg 2401 cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat 2461 ccgctagcgc taccggactc agatctcgag ctcaagcttc GATATCCAgt cgaccAGAga 2521 attcGCCACC ATGGAATACG CCTCTGACGC TTCACTGGAC CCCGAAGCCC CGTGGCCTCC 2581 CGCGCCCCGC GCTCGCGCCT GCCGCGTACT GCCTTGGGCC CTGGTCGCGG GGCTGCTGCT 2641 GCTGCTGCTG CTCGCTGCCG CCTGCGCCGT CTTCCTCGCC TGCCCCTGGG CCGTGTCCGG 2701 GGCTCGCGCC TCGCCCGGCT CCGCGGCCAG CCCGAGACTC CGCGAGGGTC CCGAGCTTTC 2761 GCCCGACGAT CCCGCCGGCC TCTTGGACCT GCGGCAGGGC ATGTTTGCGC AGCTGGTGGC 2821 CCAAAATGTT CTGCTGATCG ATGGGCCCCT GAGCTGGTAC AGTGACCCAG GCCTGGCAGG 2881 CGTGTCCCTG ACGGGGGGCC TGAGCTACAA AGAGGACACG AAGGAGCTGG TGGTGGCCAA 2941 GGCTGGAGTC TACTATGTCT TCTTTCAACT AGAGCTGCGG CGCGTGGTGG CCGGCGAGGG 3001 CTCAGGCTCC GTTTCACTTG CGCTGCACCT GCAGCCACTG CGCTCTGCTG CTGGGGCCGC 3061 CGCCCTGGCT TTGACCGTGG ACCTGCCACC CGCCTCCTCC GAGGCTCGGA ACTCGGCCTT 3121 CGGTTTCCAG GGCCGCTTGC TGCACCTGAG TGCCGGCCAG CGCCTGGGCG TCCATCTTCA 3181 CACTGAGGCC AGGGCACGCC ATGCCTGGCA GCTTACCCAG GGCGCCACAG TCTTGGGACT 3241 CTTCCGGGTG ACCCCCGAAA TCCCAGCCGG ACTCCCTTCA CCGAGGTCGG AATAAGGATC 3301 Caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc 3361 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg 3421 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt 3481 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac 3541 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc 3601 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct 3661 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct 3721 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct 3781 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct 3841 ccgccttcgc cctcagacga gtcggatctc cctttggccg cctccccgcc tggtaccttt 3901 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg 3961 actggaaggg ctaattcact cccaacgaaG ataagatctg ctttttgctt gtactgggtc 4021 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 4081 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 4141 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag 4201 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag 4261 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 4321 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 4381 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccag ttccgcccat 4441 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc 4501 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagactt ttgcagagac 4561 ggcccaaatt cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca 4621 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg 4681 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg 4741 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc 4801 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta 4861 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag 4921 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 4981 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 5041 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 5101 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 5161 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 5221 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 5281 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 5341 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 5401 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt 5461 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 5521 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 5581 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg 5641 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt 5701 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt 5761 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc 5821 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg 5881 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc 5941 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg 6001 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca 6061 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga 6121 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct 6181 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg 6241 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca 6301 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata 6361 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct 6421 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact 6481 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa 6541 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc 6601 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga 6661 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga 6721 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg 6781 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac 6841 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc 6901 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca 6961 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg 7021 agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga 7081 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga 7141 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc 7201 aagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.