pLV2-U6-AURKB-shRNA1-CMV-mCher

pLV2-U6-AURKB-shRNA1-CMV-mCherry-Luc-Puro plasmid

PVT96887

pLV2-U6-AURKB-shRNA1-CMV-mCherry-Luc-Puro plasmid Information

Plasmid name: pLV2-U6-AURKB(human)-shRNA1-CMV-mCherry-Luc-Puro
Alias: GGTGATTCACAGAGACATA
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Interference silence
Fragment type: shRNA
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Red
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction:

pLV2-U6-AURKB-shRNA1-CMV-mCherry-Luc-Puro plasmid Description

pLV2-U6-AURKB-shRNA1-CMV-mCherry-Luc-Puro This is a plasmid suitable for interference silencing in mammalian cells, and it can also be used for lentivirus packaging.

pLV2-U6-AURKB-shRNA1-CMV-mCherry-Luc-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgatctgggc aggaagaggg cctatttccc atgattcctt 2221 catatttgca tatacgatac aaggctgtta gagagataat tagaattaat ttgactgtaa 2281 acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg 2341 cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt 2401 tcgatttctt ggctttatat atcttgtgga aaggacgagg atccGGGTGA TTCACAGAGA 2461 CATAttcaag agaTATGTCT CTGTGAATCA CCttttttga attctagtta ttaatagtaa 2521 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg 2581 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg 2641 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta 2701 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt 2761 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac 2821 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt 2881 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac 2941 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt 3001 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat 3061 ataagcagag ctggtttagt gaaccgtcag atccgctagc gctaccggtc gccaccatgg 3121 tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc 3181 acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc 3241 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg 3301 cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg 3361 ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga 3421 tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg 3481 agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc 3541 agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc 3601 tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg 3661 tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca 3721 tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg 3781 ccgagggccg ccactccacc ggcggcatgg acgagctgta caaggGaGGT GGAGGTAGTG 3841 GAGGAGGCGG AtcAatggaa gatgccaaaa acattaagaa gggcccagcg ccattctacc 3901 cactcgaaga cgggaccgcc ggcgagcagc tgcacaaagc catgaagcgc tacgccctgg 3961 tgcccggcac catcgccttt accgacgcac atatcgaggt ggacattacc tacgccgagt 4021 acttcgagat gagcgttcgg ctggcagaag ctatgaagcg ctatgggctg aatacaaacc 4081 atcggatcgt ggtgtgcagc gagaatagct tgcagttctt catgcccgtg ttgggtgccc 4141 tgttcatcgg tgtggctgtg gccccagcta acgacatcta caacgagcgc gagctgctga 4201 acagcatggg catcagccag cccaccgtcg tattcgtgag caagaaaggg ctgcaaaaga 4261 tcctcaacgt gcaaaagaag ctaccgatca tacaaaagat catcatcatg gatagcaaga 4321 ccgactacca gggcttccaa agcatgtaca ccttcgtgac ttcccatttg ccacccggct 4381 tcaacgagta cgacttcgtg cccgagagct tcgaccggga caaaaccatc gccctgatca 4441 tgaacagtag tggcagtacc ggattgccca agggcgtagc cctaccgcac cgcaccgctt 4501 gtgtccgatt cagtcatgcc cgcgacccca tcttcggcaa ccagatcatc cccgacaccg 4561 ctatcctcag cgtggtgcca tttcaccacg gcttcggcat gttcaccacg ctgggctact 4621 tgatctgcgg ctttcgggtc gtgctcatgt accgcttcga ggaggagcta ttcttgcgca 4681 gcttgcaaga ctataagatt caatctgccc tgctggtgcc cacactattt agcttcttcg 4741 ctaagagcac tctcatcgac aagtacgacc taagcaactt gcacgagatc gccagcggcg 4801 gggcgccgct cagcaaggag gtaggtgagg ccgtggccaa acgcttccac ctaccaggca 4861 tccgccaggg ctacggcctg acagaaacaa ccagcgccat tctgatcacc cccgaagggg 4921 acgacaagcc tggcgcagta ggcaaggtgg tgcccttctt cgaggctaag gtggtggact 4981 tggacaccgg taagacactg ggtgtgaacc agcgcggcga gctgtgcgtc cgtggcccca 5041 tgatcatgag cggctacgtt aacaaccccg aggctacaaa cgctctcatc gacaaggacg 5101 gctggctgca cagcggcgac atcgcctact gggacgagga cgagcacttc ttcatcgtgg 5161 accggctgaa gagcctgatc aaatacaagg gctaccaggt agccccagcc gaactggaga 5221 gcatcctgct gcaacacccc aacatcttcg acgccggggt cgccggcctg cccgacgacg 5281 atgccggcga gctgcccgcc gcagtcgtcg tgctggaaca cggtaaaacc atgaccgaga 5341 aggagatcgt ggactatgtg gccagccagg ttacaaccgc caagaagctg cgcggtggtg 5401 ttgtgttcgt ggacgaggtg cctaaaggac tgaccggcaa gttggacgcc cgcaagatcc 5461 gcgagattct cattaaggcc aagaagggcg gcaagatcgc cgtgtaactc gagataattc 5521 taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg 5581 ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt 5641 aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta 5701 gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta 5761 gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc 5821 ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga 5881 aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg 5941 tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc 6001 tcttcctcat ctccgggcct ttcgacctgc agcccaagct taccatgacc gagtacaagc 6061 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg 6121 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg 6181 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg 6241 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg 6301 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc 6361 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg 6421 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc 6481 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc 6541 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg 6601 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaacgcgt ctggaacaat 6661 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct 6721 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg 6781 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg 6841 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt 6901 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt 6961 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg 7021 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc 7081 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat 7141 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc 7201 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattaattct 7261 gcagtcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg 7321 ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc 7381 aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag 7441 aaaagagggg actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt 7501 ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg 7561 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg 7621 tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga 7681 tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc 7741 acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca 7801 agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc 7861 gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta 7921 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa 7981 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac 8041 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt 8101 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggcct 8161 tgacattgct agcgtttacc gtcgacctct agctagagct tggcgtaatc atggtcatag 8221 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc 8281 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc 8341 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa 8401 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg 8461 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 8521 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 8581 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 8641 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 8701 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 8761 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 8821 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 8881 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 8941 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 9001 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca 9061 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 9121 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 9181 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 9241 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 9301 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 9361 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 9421 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 9481 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat 9541 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 9601 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 9661 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt 9721 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg 9781 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc 9841 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc 9901 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg 9961 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga 10021 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta 10081 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct 10141 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag 10201 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga 10261 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat 10321 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg 10381 ggagatcaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 10441 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 10501 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact 10561 tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg 10621 tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa 10681 cttagtagtt tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.