pX330-U6-sgRNA-Cas9-Neo plasmi

pX330-U6-sgRNA-Cas9-Neo plasmid

PVT96577

pX330-U6-sgRNA-Cas9-Neo plasmid Information

Plasmid name: pX330-U6-sgRNA-Cas9-Neo
Alias: -
Gene lenght:
Host: Mammalian cells
Use(s): Gene editing
Fragment type: CRISPR
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction:

pX330-U6-sgRNA-Cas9-Neo plasmid Description

pX330-U6-sgRNA-Cas9-Neo is a plasmid suitable for gene editing in mammalian cells.

pX330-U6-sgRNA-Cas9-Neo plasmid Sequence

1 TTTATTTTTT AATTATTTTG TGCAGCGATG GGGGCGGGGG GGGGGGGGGG GCGCGCGCCA 61 GGCGGGGCGG GGCGGGGCGA GGGGCGGGGC GGGGCGAGGC GGAGAGGTGC GGCGGCAGCC 121 AATCAGAGCG GCGCGCTCCG AAAGTTTCCT TTTATGGCGA GGCGGCGGCG GCGGCGGCCC 181 TATAAAAAGC GAAGCGCGCG GCGGGCGGGA GTCGCTGCGC GCTGCCTTCG CCCCGTGCCC 241 CGCTCCGCCG CCGCCTCGCG CCGCCCGCCC CGGCTCTGAC TGACCGCGTT ACTCCCACAG 301 GTGAGCGGGC GGGACGGCCC TTCTCCTCCG GGCTGTAATT AGCTGAGCAA GAGGTAAGGG 361 TTTAAGGGAT GGTTGGTTGG TGGGGTATTA ATGTTTAATT ACCTGGAGCA CCTGCCTGAA 421 ATCACTTTTT TTCAGGTTGG ACCGGTGCCA CCATGGACTA TAAGGACCAC GACGGAGACT 481 ACAAGGATCA TGATATTGAT TACAAAGACG ATGACGATAA GATGGCCCCA AAGAAGAAGC 541 GGAAGGTCGG TATCCACGGA GTCCCAGCAG CCGACAAGAA GTACAGCATC GGCCTGGACA 601 TCGGCACCAA CTCTGTGGGC TGGGCCGTGA TCACCGACGA GTACAAGGTG CCCAGCAAGA 661 AATTCAAGGT GCTGGGCAAC ACCGACCGGC ACAGCATCAA GAAGAACCTG ATCGGAGCCC 721 TGCTGTTCGA CAGCGGCGAA ACAGCCGAGG CCACCCGGCT GAAGAGAACC GCCAGAAGAA 781 GATACACCAG ACGGAAGAAC CGGATCTGCT ATCTGCAAGA GATCTTCAGC AACGAGATGG 841 CCAAGGTGGA CGACAGCTTC TTCCACAGAC TGGAAGAGTC CTTCCTGGTG GAAGAGGATA 901 AGAAGCACGA GCGGCACCCC ATCTTCGGCA ACATCGTGGA CGAGGTGGCC TACCACGAGA 961 AGTACCCCAC CATCTACCAC CTGAGAAAGA AACTGGTGGA CAGCACCGAC AAGGCCGACC 1021 TGCGGCTGAT CTATCTGGCC CTGGCCCACA TGATCAAGTT CCGGGGCCAC TTCCTGATCG 1081 AGGGCGACCT GAACCCCGAC AACAGCGACG TGGACAAGCT GTTCATCCAG CTGGTGCAGA 1141 CCTACAACCA GCTGTTCGAG GAAAACCCCA TCAACGCCAG CGGCGTGGAC GCCAAGGCCA 1201 TCCTGTCTGC CAGACTGAGC AAGAGCAGAC GGCTGGAAAA TCTGATCGCC CAGCTGCCCG 1261 GCGAGAAGAA GAATGGCCTG TTCGGAAACC TGATTGCCCT GAGCCTGGGC CTGACCCCCA 1321 ACTTCAAGAG CAACTTCGAC CTGGCCGAGG ATGCCAAACT GCAGCTGAGC AAGGACACCT 1381 ACGACGACGA CCTGGACAAC CTGCTGGCCC AGATCGGCGA CCAGTACGCC GACCTGTTTC 1441 TGGCCGCCAA GAACCTGTCC GACGCCATCC TGCTGAGCGA CATCCTGAGA GTGAACACCG 1501 AGATCACCAA GGCCCCCCTG AGCGCCTCTA TGATCAAGAG ATACGACGAG CACCACCAGG 1561 ACCTGACCCT GCTGAAAGCT CTCGTGCGGC AGCAGCTGCC TGAGAAGTAC AAAGAGATTT 1621 TCTTCGACCA GAGCAAGAAC GGCTACGCCG GCTACATTGA CGGCGGAGCC AGCCAGGAAG 1681 AGTTCTACAA GTTCATCAAG CCCATCCTGG AAAAGATGGA CGGCACCGAG GAACTGCTCG 1741 TGAAGCTGAA CAGAGAGGAC CTGCTGCGGA AGCAGCGGAC CTTCGACAAC GGCAGCATCC 1801 CCCACCAGAT CCACCTGGGA GAGCTGCACG CCATTCTGCG GCGGCAGGAA GATTTTTACC 1861 CATTCCTGAA GGACAACCGG GAAAAGATCG AGAAGATCCT GACCTTCCGC ATCCCCTACT 1921 ACGTGGGCCC TCTGGCCAGG GGAAACAGCA GATTCGCCTG GATGACCAGA AAGAGCGAGG 1981 AAACCATCAC CCCCTGGAAC TTCGAGGAAG TGGTGGACAA GGGCGCTTCC GCCCAGAGCT 2041 TCATCGAGCG GATGACCAAC TTCGATAAGA ACCTGCCCAA CGAGAAGGTG CTGCCCAAGC 2101 ACAGCCTGCT GTACGAGTAC TTCACCGTGT ATAACGAGCT GACCAAAGTG AAATACGTGA 2161 CCGAGGGAAT GAGAAAGCCC GCCTTCCTGA GCGGCGAGCA GAAAAAGGCC ATCGTGGACC 2221 TGCTGTTCAA GACCAACCGG AAAGTGACCG TGAAGCAGCT GAAAGAGGAC TACTTCAAGA 2281 AAATCGAGTG CTTCGACTCC GTGGAAATCT CCGGCGTGGA AGATCGGTTC AACGCCTCCC 2341 TGGGCACATA CCACGATCTG CTGAAAATTA TCAAGGACAA GGACTTCCTG GACAATGAGG 2401 AAAACGAGGA CATTCTGGAA GATATCGTGC TGACCCTGAC ACTGTTTGAG GACAGAGAGA 2461 TGATCGAGGA ACGGCTGAAA ACCTATGCCC ACCTGTTCGA CGACAAAGTG ATGAAGCAGC 2521 TGAAGCGGCG GAGATACACC GGCTGGGGCA GGCTGAGCCG GAAGCTGATC AACGGCATCC 2581 GGGACAAGCA GTCCGGCAAG ACAATCCTGG ATTTCCTGAA GTCCGACGGC TTCGCCAACA 2641 GAAACTTCAT GCAGCTGATC CACGACGACA GCCTGACCTT TAAAGAGGAC ATCCAGAAAG 2701 CCCAGGTGTC CGGCCAGGGC GATAGCCTGC ACGAGCACAT TGCCAATCTG GCCGGCAGCC 2761 CCGCCATTAA GAAGGGCATC CTGCAGACAG TGAAGGTGGT GGACGAGCTC GTGAAAGTGA 2821 TGGGCCGGCA CAAGCCCGAG AACATCGTGA TCGAAATGGC CAGAGAGAAC CAGACCACCC 2881 AGAAGGGACA GAAGAACAGC CGCGAGAGAA TGAAGCGGAT CGAAGAGGGC ATCAAAGAGC 2941 TGGGCAGCCA GATCCTGAAA GAACACCCCG TGGAAAACAC CCAGCTGCAG AACGAGAAGC 3001 TGTACCTGTA CTACCTGCAG AATGGGCGGG ATATGTACGT GGACCAGGAA CTGGACATCA 3061 ACCGGCTGTC CGACTACGAT GTGGACCATA TCGTGCCTCA GAGCTTTCTG AAGGACGACT 3121 CCATCGACAA CAAGGTGCTG ACCAGAAGCG ACAAGAACCG GGGCAAGAGC GACAACGTGC 3181 CCTCCGAAGA GGTCGTGAAG AAGATGAAGA ACTACTGGCG GCAGCTGCTG AACGCCAAGC 3241 TGATTACCCA GAGAAAGTTC GACAATCTGA CCAAGGCCGA GAGAGGCGGC CTGAGCGAAC 3301 TGGATAAGGC CGGCTTCATC AAGAGACAGC TGGTGGAAAC CCGGCAGATC ACAAAGCACG 3361 TGGCACAGAT CCTGGACTCC CGGATGAACA CTAAGTACGA CGAGAATGAC AAGCTGATCC 3421 GGGAAGTGAA AGTGATCACC CTGAAGTCCA AGCTGGTGTC CGATTTCCGG AAGGATTTCC 3481 AGTTTTACAA AGTGCGCGAG ATCAACAACT ACCACCACGC CCACGACGCC TACCTGAACG 3541 CCGTCGTGGG AACCGCCCTG ATCAAAAAGT ACCCTAAGCT GGAAAGCGAG TTCGTGTACG 3601 GCGACTACAA GGTGTACGAC GTGCGGAAGA TGATCGCCAA GAGCGAGCAG GAAATCGGCA 3661 AGGCTACCGC CAAGTACTTC TTCTACAGCA ACATCATGAA CTTTTTCAAG ACCGAGATTA 3721 CCCTGGCCAA CGGCGAGATC CGGAAGCGGC CTCTGATCGA GACAAACGGC GAAACCGGGG 3781 AGATCGTGTG GGATAAGGGC CGGGATTTTG CCACCGTGCG GAAAGTGCTG AGCATGCCCC 3841 AAGTGAATAT CGTGAAAAAG ACCGAGGTGC AGACAGGCGG CTTCAGCAAA GAGTCTATCC 3901 TGCCCAAGAG GAACAGCGAT AAGCTGATCG CCAGAAAGAA GGACTGGGAC CCTAAGAAGT 3961 ACGGCGGCTT CGACAGCCCC ACCGTGGCCT ATTCTGTGCT GGTGGTGGCC AAAGTGGAAA 4021 AGGGCAAGTC CAAGAAACTG AAGAGTGTGA AAGAGCTGCT GGGGATCACC ATCATGGAAA 4081 GAAGCAGCTT CGAGAAGAAT CCCATCGACT TTCTGGAAGC CAAGGGCTAC AAAGAAGTGA 4141 AAAAGGACCT GATCATCAAG CTGCCTAAGT ACTCCCTGTT CGAGCTGGAA AACGGCCGGA 4201 AGAGAATGCT GGCCTCTGCC GGCGAACTGC AGAAGGGAAA CGAACTGGCC CTGCCCTCCA 4261 AATATGTGAA CTTCCTGTAC CTGGCCAGCC ACTATGAGAA GCTGAAGGGC TCCCCCGAGG 4321 ATAATGAGCA GAAACAGCTG TTTGTGGAAC AGCACAAGCA CTACCTGGAC GAGATCATCG 4381 AGCAGATCAG CGAGTTCTCC AAGAGAGTGA TCCTGGCCGA CGCTAATCTG GACAAAGTGC 4441 TGTCCGCCTA CAACAAGCAC CGGGATAAGC CCATCAGAGA GCAGGCCGAG AATATCATCC 4501 ACCTGTTTAC CCTGACCAAT CTGGGAGCCC CTGCCGCCTT CAAGTACTTT GACACCACCA 4561 TCGACCGGAA GAGGTACACC AGCACCAAAG AGGTGCTGGA CGCCACCCTG ATCCACCAGA 4621 GCATCACCGG CCTGTACGAG ACACGGATCG ACCTGTCTCA GCTGGGAGGC GACAAAAGGC 4681 CGGCGGCCAC GAAAAAGGCC GGCCAGGCAA AAAAGAAAAA GGAATTCGCA ACAAACTTCT 4741 CTCTGCTGAA ACAAGCCGGA GATGTCGAAG AGAATCCTGG ACCGATTGAA CAAGATGGAT 4801 TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC 4861 AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC 4921 TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC 4981 TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG 5041 CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC 5101 TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG 5161 ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC 5221 GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC 5281 CAGCCGAACT GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA 5341 CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA 5401 TCGACTGTGG CCGGCTGGGT GTGGCGGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG 5461 ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG 5521 CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGACTAG 5581 AGCTCGCTGA TCAGCCTCGA CTGTGCCTTC TAGTTGCCAG CCATCTGTTG TTTGCCCCTC 5641 CCCCGTGCCT TCCTTGACCC TGGAAGGTGC CACTCCCACT GTCCTTTCCT AATAAAATGA 5701 GGAAATTGCA TCGCATTGTC TGAGTAGGTG TCATTCTATT CTGGGGGGTG GGGTGGGGCA 5761 GGACAGCAAG GGGGAGGATT GGGAAGAGAA TAGCAGGCAT GCTGGGGAGC GGCCGCAGGA 5821 ACCCCTAGTG ATGGAGTTGG CCACTCCCTC TCTGCGCGCT CGCTCGCTCA CTGAGGCCGG 5881 GCGACCAAAG GTCGCCCGAC GCCCGGGCTT TGCCCGGGCG GCCTCAGTGA GCGAGCGAGC 5941 GCGCAGCTGC CTGCAGGGGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT 6001 TTCACACCGC ATACGTCAAA GCAACCATAG TACGCGCCCT GTAGCGGCGC ATTAAGCGCG 6061 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCTT AGCGCCCGCT 6121 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 6181 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 6241 CTTGATTTGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 6301 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 6361 AACTCTATCT CGGGCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGTCTATTGG 6421 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT 6481 ACAATTTTAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGCCC 6541 CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT 6601 TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA 6661 CCGAAACGCG CGAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG 6721 ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT 6781 ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA 6841 TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC 6901 CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG 6961 AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC 7021 AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT 7081 TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC 7141 GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG 7201 CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT 7261 AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT 7321 TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA 7381 GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC 7441 AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG 7501 GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT 7561 GCTGATAAAT CTGGAGCCGG TGAGCGTGGA AGCCGCGGTA TCATTGCAGC ACTGGGGCCA 7621 GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT 7681 GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA 7741 GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG 7801 ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG 7861 TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT 7921 CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG 7981 CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA 8041 CCAAATACTG TTCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA 8101 CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG 8161 TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC 8221 TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA 8281 TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG 8341 TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC 8401 GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG 8461 TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG 8521 TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTGAGGGC CTATTTCCCA TGATTCCTTC 8581 ATATTTGCAT ATACGATACA AGGCTGTTAG AGAGATAATT GGAATTAATT TGACTGTAAA 8641 CACAAAGATA TTAGTACAAA ATACGTGACG TAGAAAGTAA TAATTTCTTG GGTAGTTTGC 8701 AGTTTTAAAA TTATGTTTTA AAATGGACTA TCATATGCTT ACCGTAACTT GAAAGTATTT 8761 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAAAC ACCGGGTCTT CGAGAAGACC 8821 TGTTTTAGAG CTAGAAATAG CAAGTTAAAA TAAGGCTAGT CCGTTATCAA CTTGAAAAAG 8881 TGGCACCGAG TCGGTGCTTT TTTGTTTTAG AGCTAGAAAT AGCAAGTTAA AATAAGGCTA 8941 GTCCGTTTTT AGCGCGTGCG CCAATTCTGC AGACAAATGG CTCTAGAGGT ACCCGTTACA 9001 TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG ACCCCCGCCC ATTGACGTCA 9061 ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT 9121 GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT 9181 GACGGTAAAT GGCCCGCCTG GCATTGTGCC CAGTACATGA CCTTATGGGA CTTTCCTACT 9241 TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TCGAGGTGAG CCCCACGTTC 9301 TGCTTCACTC TCCCCATCTC CCCCCCCTCC CCACCCCCAA TTTTGTATTT A

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.