pX330-U6-CALCA-sgRNA1-CBH-Cas9

pX330-U6-CALCA-sgRNA1-CBH-Cas9-P2A-EGFP plasmid

PVT96074

pX330-U6-CALCA-sgRNA1-CBH-Cas9-P2A-EGFP plasmid Information

Plasmid name: pX330-U6-CALCA(human)-sgRNA1-CBH-Cas9-P2A-EGFP
Alias: AGACCCGGCCACGCTCAGTG
Gene lenght:
Host: Mammalian cells
Use(s): Gene editing
Fragment type: CRISPR
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Green
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction:

pX330-U6-CALCA-sgRNA1-CBH-Cas9-P2A-EGFP plasmid Description

pX330-U6-CALCA-sgRNA1-CBH-Cas9-P2A-EGFP is a plasmid suitable for gene editing in mammalian cells.

pX330-U6-CALCA-sgRNA1-CBH-Cas9-P2A-EGFP plasmid Sequence

1 TTTATTTTTT AATTATTTTG TGCAGCGATG GGGGCGGGGG GGGGGGGGGG GCGCGCGCCA 61 GGCGGGGCGG GGCGGGGCGA GGGGCGGGGC GGGGCGAGGC GGAGAGGTGC GGCGGCAGCC 121 AATCAGAGCG GCGCGCTCCG AAAGTTTCCT TTTATGGCGA GGCGGCGGCG GCGGCGGCCC 181 TATAAAAAGC GAAGCGCGCG GCGGGCGGGA GTCGCTGCGC GCTGCCTTCG CCCCGTGCCC 241 CGCTCCGCCG CCGCCTCGCG CCGCCCGCCC CGGCTCTGAC TGACCGCGTT ACTCCCACAG 301 GTGAGCGGGC GGGACGGCCC TTCTCCTCCG GGCTGTAATT AGCTGAGCAA GAGGTAAGGG 361 TTTAAGGGAT GGTTGGTTGG TGGGGTATTA ATGTTTAATT ACCTGGAGCA CCTGCCTGAA 421 ATCACTTTTT TTCAGGTTGG ACCGGTGCCA CCATGGACTA TAAGGACCAC GACGGAGACT 481 ACAAGGATCA TGATATTGAT TACAAAGACG ATGACGATAA GATGGCCCCA AAGAAGAAGC 541 GGAAGGTCGG TATCCACGGA GTCCCAGCAG CCGACAAGAA GTACAGCATC GGCCTGGACA 601 TCGGCACCAA CTCTGTGGGC TGGGCCGTGA TCACCGACGA GTACAAGGTG CCCAGCAAGA 661 AATTCAAGGT GCTGGGCAAC ACCGACCGGC ACAGCATCAA GAAGAACCTG ATCGGAGCCC 721 TGCTGTTCGA CAGCGGCGAA ACAGCCGAGG CCACCCGGCT GAAGAGAACC GCCAGAAGAA 781 GATACACCAG ACGGAAGAAC CGGATCTGCT ATCTGCAAGA GATCTTCAGC AACGAGATGG 841 CCAAGGTGGA CGACAGCTTC TTCCACAGAC TGGAAGAGTC CTTCCTGGTG GAAGAGGATA 901 AGAAGCACGA GCGGCACCCC ATCTTCGGCA ACATCGTGGA CGAGGTGGCC TACCACGAGA 961 AGTACCCCAC CATCTACCAC CTGAGAAAGA AACTGGTGGA CAGCACCGAC AAGGCCGACC 1021 TGCGGCTGAT CTATCTGGCC CTGGCCCACA TGATCAAGTT CCGGGGCCAC TTCCTGATCG 1081 AGGGCGACCT GAACCCCGAC AACAGCGACG TGGACAAGCT GTTCATCCAG CTGGTGCAGA 1141 CCTACAACCA GCTGTTCGAG GAAAACCCCA TCAACGCCAG CGGCGTGGAC GCCAAGGCCA 1201 TCCTGTCTGC CAGACTGAGC AAGAGCAGAC GGCTGGAAAA TCTGATCGCC CAGCTGCCCG 1261 GCGAGAAGAA GAATGGCCTG TTCGGAAACC TGATTGCCCT GAGCCTGGGC CTGACCCCCA 1321 ACTTCAAGAG CAACTTCGAC CTGGCCGAGG ATGCCAAACT GCAGCTGAGC AAGGACACCT 1381 ACGACGACGA CCTGGACAAC CTGCTGGCCC AGATCGGCGA CCAGTACGCC GACCTGTTTC 1441 TGGCCGCCAA GAACCTGTCC GACGCCATCC TGCTGAGCGA CATCCTGAGA GTGAACACCG 1501 AGATCACCAA GGCCCCCCTG AGCGCCTCTA TGATCAAGAG ATACGACGAG CACCACCAGG 1561 ACCTGACCCT GCTGAAAGCT CTCGTGCGGC AGCAGCTGCC TGAGAAGTAC AAAGAGATTT 1621 TCTTCGACCA GAGCAAGAAC GGCTACGCCG GCTACATTGA CGGCGGAGCC AGCCAGGAAG 1681 AGTTCTACAA GTTCATCAAG CCCATCCTGG AAAAGATGGA CGGCACCGAG GAACTGCTCG 1741 TGAAGCTGAA CAGAGAGGAC CTGCTGCGGA AGCAGCGGAC CTTCGACAAC GGCAGCATCC 1801 CCCACCAGAT CCACCTGGGA GAGCTGCACG CCATTCTGCG GCGGCAGGAA GATTTTTACC 1861 CATTCCTGAA GGACAACCGG GAAAAGATCG AGAAGATCCT GACCTTCCGC ATCCCCTACT 1921 ACGTGGGCCC TCTGGCCAGG GGAAACAGCA GATTCGCCTG GATGACCAGA AAGAGCGAGG 1981 AAACCATCAC CCCCTGGAAC TTCGAGGAAG TGGTGGACAA GGGCGCTTCC GCCCAGAGCT 2041 TCATCGAGCG GATGACCAAC TTCGATAAGA ACCTGCCCAA CGAGAAGGTG CTGCCCAAGC 2101 ACAGCCTGCT GTACGAGTAC TTCACCGTGT ATAACGAGCT GACCAAAGTG AAATACGTGA 2161 CCGAGGGAAT GAGAAAGCCC GCCTTCCTGA GCGGCGAGCA GAAAAAGGCC ATCGTGGACC 2221 TGCTGTTCAA GACCAACCGG AAAGTGACCG TGAAGCAGCT GAAAGAGGAC TACTTCAAGA 2281 AAATCGAGTG CTTCGACTCC GTGGAAATCT CCGGCGTGGA AGATCGGTTC AACGCCTCCC 2341 TGGGCACATA CCACGATCTG CTGAAAATTA TCAAGGACAA GGACTTCCTG GACAATGAGG 2401 AAAACGAGGA CATTCTGGAA GATATCGTGC TGACCCTGAC ACTGTTTGAG GACAGAGAGA 2461 TGATCGAGGA ACGGCTGAAA ACCTATGCCC ACCTGTTCGA CGACAAAGTG ATGAAGCAGC 2521 TGAAGCGGCG GAGATACACC GGCTGGGGCA GGCTGAGCCG GAAGCTGATC AACGGCATCC 2581 GGGACAAGCA GTCCGGCAAG ACAATCCTGG ATTTCCTGAA GTCCGACGGC TTCGCCAACA 2641 GAAACTTCAT GCAGCTGATC CACGACGACA GCCTGACCTT TAAAGAGGAC ATCCAGAAAG 2701 CCCAGGTGTC CGGCCAGGGC GATAGCCTGC ACGAGCACAT TGCCAATCTG GCCGGCAGCC 2761 CCGCCATTAA GAAGGGCATC CTGCAGACAG TGAAGGTGGT GGACGAGCTC GTGAAAGTGA 2821 TGGGCCGGCA CAAGCCCGAG AACATCGTGA TCGAAATGGC CAGAGAGAAC CAGACCACCC 2881 AGAAGGGACA GAAGAACAGC CGCGAGAGAA TGAAGCGGAT CGAAGAGGGC ATCAAAGAGC 2941 TGGGCAGCCA GATCCTGAAA GAACACCCCG TGGAAAACAC CCAGCTGCAG AACGAGAAGC 3001 TGTACCTGTA CTACCTGCAG AATGGGCGGG ATATGTACGT GGACCAGGAA CTGGACATCA 3061 ACCGGCTGTC CGACTACGAT GTGGACCATA TCGTGCCTCA GAGCTTTCTG AAGGACGACT 3121 CCATCGACAA CAAGGTGCTG ACCAGAAGCG ACAAGAACCG GGGCAAGAGC GACAACGTGC 3181 CCTCCGAAGA GGTCGTGAAG AAGATGAAGA ACTACTGGCG GCAGCTGCTG AACGCCAAGC 3241 TGATTACCCA GAGAAAGTTC GACAATCTGA CCAAGGCCGA GAGAGGCGGC CTGAGCGAAC 3301 TGGATAAGGC CGGCTTCATC AAGAGACAGC TGGTGGAAAC CCGGCAGATC ACAAAGCACG 3361 TGGCACAGAT CCTGGACTCC CGGATGAACA CTAAGTACGA CGAGAATGAC AAGCTGATCC 3421 GGGAAGTGAA AGTGATCACC CTGAAGTCCA AGCTGGTGTC CGATTTCCGG AAGGATTTCC 3481 AGTTTTACAA AGTGCGCGAG ATCAACAACT ACCACCACGC CCACGACGCC TACCTGAACG 3541 CCGTCGTGGG AACCGCCCTG ATCAAAAAGT ACCCTAAGCT GGAAAGCGAG TTCGTGTACG 3601 GCGACTACAA GGTGTACGAC GTGCGGAAGA TGATCGCCAA GAGCGAGCAG GAAATCGGCA 3661 AGGCTACCGC CAAGTACTTC TTCTACAGCA ACATCATGAA CTTTTTCAAG ACCGAGATTA 3721 CCCTGGCCAA CGGCGAGATC CGGAAGCGGC CTCTGATCGA GACAAACGGC GAAACCGGGG 3781 AGATCGTGTG GGATAAGGGC CGGGATTTTG CCACCGTGCG GAAAGTGCTG AGCATGCCCC 3841 AAGTGAATAT CGTGAAAAAG ACCGAGGTGC AGACAGGCGG CTTCAGCAAA GAGTCTATCC 3901 TGCCCAAGAG GAACAGCGAT AAGCTGATCG CCAGAAAGAA GGACTGGGAC CCTAAGAAGT 3961 ACGGCGGCTT CGACAGCCCC ACCGTGGCCT ATTCTGTGCT GGTGGTGGCC AAAGTGGAAA 4021 AGGGCAAGTC CAAGAAACTG AAGAGTGTGA AAGAGCTGCT GGGGATCACC ATCATGGAAA 4081 GAAGCAGCTT CGAGAAGAAT CCCATCGACT TTCTGGAAGC CAAGGGCTAC AAAGAAGTGA 4141 AAAAGGACCT GATCATCAAG CTGCCTAAGT ACTCCCTGTT CGAGCTGGAA AACGGCCGGA 4201 AGAGAATGCT GGCCTCTGCC GGCGAACTGC AGAAGGGAAA CGAACTGGCC CTGCCCTCCA 4261 AATATGTGAA CTTCCTGTAC CTGGCCAGCC ACTATGAGAA GCTGAAGGGC TCCCCCGAGG 4321 ATAATGAGCA GAAACAGCTG TTTGTGGAAC AGCACAAGCA CTACCTGGAC GAGATCATCG 4381 AGCAGATCAG CGAGTTCTCC AAGAGAGTGA TCCTGGCCGA CGCTAATCTG GACAAAGTGC 4441 TGTCCGCCTA CAACAAGCAC CGGGATAAGC CCATCAGAGA GCAGGCCGAG AATATCATCC 4501 ACCTGTTTAC CCTGACCAAT CTGGGAGCCC CTGCCGCCTT CAAGTACTTT GACACCACCA 4561 TCGACCGGAA GAGGTACACC AGCACCAAAG AGGTGCTGGA CGCCACCCTG ATCCACCAGA 4621 GCATCACCGG CCTGTACGAG ACACGGATCG ACCTGTCTCA GCTGGGAGGC GACAAAAGGC 4681 CGGCGGCCAC GAAAAAGGCC GGCCAGGCAA AAAAGAAAAA GGAATTCGCA ACAAACTTCT 4741 CTCTGCTGAA ACAAGCCGGA GATGTCGAAG AGAATCCTGG ACCGATGGTG TCTAAGGGCG 4801 AAGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC 4861 ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG CTGACCCTGA 4921 AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCCTGA 4981 CCTACGGCGT GCAGTGCTTC AGCCGCTACC CCGACCACAT GAAGCAGCAC GACTTCTTCA 5041 AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT CTTCTTCAAG GACGACGGCA 5101 ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC CCTGGTGAAC CGCATCGAGC 5161 TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG GCACAAGCTG GAGTACAACT 5221 ACAACAGCCA CAACGTCTAT ATCATGGCCG ACAAGCAGAA GAACGGCATC AAGGTGAACT 5281 TCAAGATCCG CCACAACATC GAGGACGGCA GCGTGCAGCT CGCCGACCAC TACCAGCAGA 5341 ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG AGCACCCAGT 5401 CCGCCCTGAG CAAAGACCCC AACGAGAAGC GCGATCACAT GGTCCTGCTG GAGTTCGTGA 5461 CCGCCGCCGG GATCACTCTC GGCATGGACG AGCTGTACAA GTAACTAGAG CTCGCTGATC 5521 AGCCTCGACT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC CCGTGCCTTC 5581 CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA TAAAATGAGG AAATTGCATC 5641 GCATTGTCTG AGTAGGTGTC ATTCTATTCT GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG 5701 GGAGGATTGG GAAGAGAATA GCAGGCATGC TGGGGAGCGG CCGCAGGAAC CCCTAGTGAT 5761 GGAGTTGGCC ACTCCCTCTC TGCGCGCTCG CTCGCTCACT GAGGCCGGGC GACCAAAGGT 5821 CGCCCGACGC CCGGGCTTTG CCCGGGCGGC CTCAGTGAGC GAGCGAGCGC GCAGCTGCCT 5881 GCAGGGGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT 5941 ACGTCAAAGC AACCATAGTA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 6001 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCTTAG CGCCCGCTCC TTTCGCTTTC 6061 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 6121 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTTGGGT 6181 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 6241 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CTCTATCTCG 6301 GGCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG TCTATTGGTT AAAAAATGAG 6361 CTGATTTAAC AAAAATTTAA CGCGAATTTT AACAAAATAT TAACGTTTAC AATTTTATGG 6421 TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGCCCCG ACACCCGCCA 6481 ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT 6541 GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG 6601 AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT 6661 TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT 6721 TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA 6781 TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT 6841 TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT 6901 GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG 6961 ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG 7021 CTATGTGGCG CGGTATTATC CCGTATTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA 7081 CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT 7141 GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC 7201 AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG 7261 GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC 7321 GACGAGCGTG ACACCACGAT GCCTGTAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT 7381 GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA 7441 GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT 7501 GGAGCCGGTG AGCGTGGAAG CCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC 7561 TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA 7621 CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC 7681 TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG 7741 ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG 7801 TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC 7861 TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG 7921 CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTT 7981 CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC 8041 CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC 8101 GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT 8161 TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT 8221 GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC 8281 GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT 8341 TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA 8401 GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT 8461 TGCTGGCCTT TTGCTCACAT GTGAGGGCCT ATTTCCCATG ATTCCTTCAT ATTTGCATAT 8521 ACGATACAAG GCTGTTAGAG AGATAATTGG AATTAATTTG ACTGTAAACA CAAAGATATT 8581 AGTACAAAAT ACGTGACGTA GAAAGTAATA ATTTCTTGGG TAGTTTGCAG TTTTAAAATT 8641 ATGTTTTAAA ATGGACTATC ATATGCTTAC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 8701 TTTATATATC TTGTGGAAAG GACGAAACAC CGGAGACCCG GCCACGCTCA GTGGTTTTAG 8761 AGCTAGAAAT AGCAAGTTAA AATAAGGCTA GTCCGTTATC AACTTGAAAA AGTGGCACCG 8821 AGTCGGTGCT TTTTTGTTTT AGAGCTAGAA ATAGCAAGTT AAAATAAGGC TAGTCCGTTT 8881 TTAGCGCGTG CGCCAATTCT GCAGACAAAT GGCTCTAGAG GTACCCGTTA CATAACTTAC 8941 GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAGTAAC 9001 GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT 9061 GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA 9121 ATGGCCCGCC TGGCATTGTG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA 9181 CATCTACGTA TTAGTCATCG CTATTACCAT GGTCGAGGTG AGCCCCACGT TCTGCTTCAC 9241 TCTCCCCATC TCCCCCCCCT CCCCACCCCC AATTTTGTAT TTA

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.