pBAMD1-4 plasmid

pBAMD1-4 plasmid

PVT91194

pBAMD1-4 plasmid Information

Plasmid name: pBAMD1-4
Alias: Plasmid
Gene lenght:
Host: bacteria
Use(s): Gene editing
Fragment type: CRISPR
Species: Empty Vector
Prokaryotic resistance: Str
Screening marker: SmR
Fluorescent labeling:
Promoter: Non
Replicon: R6K γ
Growth Strain(s): Pir1
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: pQE-R
Copy number: Low
Induction:

pBAMD1-4 plasmid Description

pBAMD1-4 A plasmid suitable for gene editing in bacteria.

pBAMD1-4 plasmid Sequence

1 tttaattaac tgtctcttat acacatcttt gtgtctcagg ccgcctaggc cgcggccgcg 61 cgaattcgag ctcggtaccc ggggatcctc tagagtcgac ctgcaggcat gcaagcttgc 121 ggccgccaaa gcccgccgaa aggcgggctt ttctgtattt aaatgaacct tgaccgaacg 181 cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttggggt 241 acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat 301 gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat 361 catgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat 421 cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg 481 cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga 541 aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag 601 cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg 661 ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg 721 tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga 781 acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca 841 ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc 901 tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg 961 caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta 1021 tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc 1081 ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt 1141 agtcggcaaa taagacaatt gtctaattaa ttgcggaccc tagaggtccc cttttttatt 1201 ttaaaaattt tttcacaaaa cggtttacaa gcataaaatc tctgaagatg tgtataagag 1261 acagactagt cttggactcc tgttgataga tccagtaatg acctcagaac tccatctgga 1321 tttgttcaga acgctcggtt gccgccgggc gttttttatt ggtgagaatc caggggtccc 1381 ctggtttaaa ctacacaagt agcgtcctga acggaacctt tcccgttttc cagaatctga 1441 tgttccatgt gacctcctaa catggtaacg ttcatgatta ccagtgcact gcatcgtgcg 1501 gcggattggg cgaaaagcgt gttttctagt gctgcgctgg gtgatccgcg tcgtaccgcg 1561 cgtctggtga atgttgcggc gcaactggcc aaatatagcg gcaaaagcat taccattagc 1621 agcgaaggca gcaaagccat gcaggaaggc gcgtatcgtt ttattcgtaa tccgaacgtg 1681 agcgcggaag cgattcgtaa agcgggtgcc atgcagaccg tgaaactggc ccaggaattt 1741 ccggaactgc tggcaattga agataccacc tctctgagct atcgtcatca ggtggcggaa 1801 gaactgggca aactgggtag cattcaggat aaaagccgtg gttggtgggt gcatagcgtg 1861 ctgctgctgg aagcgaccac ctttcgtacc gtgggcctgc tgcatcaaga atggtggatg 1921 cgtccggatg atccggcgga tgcggatgaa aaagaaagcg gcaaatggct ggccgctgct 1981 gcaacttcgc gtctgagaat gggcagcatg atgagcaacg tgattgcggt gtgcgatcgt 2041 gaagcggata ttcatgcgta tctgcaagat aaactggccc ataacgaacg ttttgtggtg 2101 cgtagcaaac atccgcgtaa agatgtggaa agcggcctgt atctgtatga tcacctgaaa 2161 aaccagccgg aactgggcgg ctatcagatt agcattccgc agaaaggcgt ggtggataaa 2221 cgtggcaaac gtaaaaaccg tccggcgcgt aaagcgagcc tgagcctgcg tagcggccgt 2281 attaccctga aacagggcaa cattaccctg aacgcggtgc tggccgaaga aattaatccg 2341 ccgaaaggcg aaaccccgct gaaatggctg ctgctgacca gcgagccggt ggaaagtctg 2401 gcccaagcgc tgcgtgtgat tgatatttat acccatcgtt ggcgcattga agaatttcac 2461 aaagcgtgga aaacgggtgc gggtgcggaa cgtcagcgta tggaagaacc ggataacctg 2521 gaacgtatgg tgagcattct gagctttgtg gcggtgcgtc tgctgcaact gcgtgaatct 2581 tttactccgc cgcaagcact gcgtgcgcag ggcctgctga aagaagcgga acacgttgaa 2641 agccagagcg cggaaaccgt gctgaccccg gatgaatgcc aactgctggg ctatctggat 2701 aaaggcaaac gcaaacgcaa agaaaaagcg ggcagcctgc aatgggcgta tatggcgatt 2761 gcgcgtctgg gcggctttat ggatagcaaa cgtaccggca ttgcgagctg gggtgcgctg 2821 tgggaaggtt gggaagcgct gcaaagcaaa ctggatggct ttctggccgc gaaagacctg 2881 atggcgcagg gcattaaaat ctaatcggcg atcgctagta gcccgcctaa tgagcgggct 2941 tttttttaat tcccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat 3001 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagcattca 3061 gcattttcgt gtggcgctga ttccgttttt tgcggcgttt tgcctgccgg tgtttgcgca 3121 tccggaaacc ctggtgaaag tgaaagatgc ggaagatcaa ctgggtgcgc gcgtgggcta 3181 tattgaactg gatctgaaca gcggcaaaat tctggaatct tttcgtccgg aagaacgttt 3241 tccgatgatg agcaccttta aagtgctgct gtgcggtgcg gttctgagcc gtgtggatgc 3301 gggccaggaa caactgggcc gtcgtattca ttatagccag aacgatctgg tggaatatag 3361 cccggtgacc gaaaaacatc tgaccgatgg catgaccgtg cgtgaactgt gcagcgcggc 3421 gattaccatg agcgataaca ccgcggcgaa cctgctgctg acgaccattg gcggtccgaa 3481 agaactgacc gcgtttctgc ataacatggg cgatcatgtg acccgtctgg atcgttggga 3541 accggaactg aacgaagcga ttccgaacga tgaacgtgat accaccatgc cggcagcaat 3601 ggcgaccacc ctgcgtaaac tgctgacggg tgagctgctg accctggcaa gccgccagca 3661 actgattgat tggatggaag cggataaagt ggcgggtccg ctgctgcgta gcgcgctgcc 3721 ggctggctgg tttattgcgg ataaaagcgg tgcgggcgaa cgtggcagcc gtggcattat 3781 tgcggcgctg ggcccggatg gtaaaccgag ccgtattgtg gtgatttata ccaccggcag 3841 ccaggcgacg atggatgaac gtaaccgtca gattgcggaa attggcgcga gcctgattaa 3901 acattggtaa accgatacaa ttaaaggctc cttttggagc cttttttttt ggacacgcgt 3961 ttgtcctttt ccgctgcata accctgcttc ggggtcatta tagcgatttt ttcggtatat 4021 ccatcctttt tcgcacgata tacaggattt tgccaaaggg ttcgtgtaga ctttccttgg 4081 tgtatccaac ggcgtcagcc gggcaggata ggtgaagtag gcccacccgc gagcgggtgt 4141 tccttcttca ctgtccctta ttcgcacctg gcggtgctca acgggaatcc tgctctgcga 4201 ggctggccgt aggccggccg atctgaagat cagcagttca acctgttgat agtacgtact 4261 aagctctcat gtttcacgta ctaagctctc atgtttaacg tactaagctc tcatgtttaa 4321 cgaactaaac cctcatggct aacgtactaa gctctcatgg ctaacgtact aagctctcat 4381 gtttcacgta ctaagctctc atgtttgaac aataaaatta atataaatca gcaacttaaa 4441 tagcctctaa ggttttaagt tttataagaa aaaaaagaat atataaggct tttaaagcct 4501 ttaaggttta acggttgtgg acaacaagcc agggatgtaa cgcactgaga agcccttaga 4561 gcctctcaaa gcaattttga gtgacacagg aacacttaac ggctgacatg gggcgcgccc 4621 agctgtctag ggcggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat 4681 aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt atttgatgcc

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.