pLV2-EF1a-Tigit-Puro plasmid

pLV2-EF1a-Tigit-Puro plasmid

PVT96125

pLV2-EF1a-Tigit-Puro plasmid Information

Plasmid name: pLV2-EF1a-Tigit(mouse)-Puro
Alias: NM_001146325.2;Vstm3
Gene lenght: 723bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: EF1a
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: EF1a-R-F
Reverse primer:
Copy number: High
Induction:

pLV2-EF1a-Tigit-Puro plasmid Description

pLV2-EF1a-Tigit-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV2-EF1a-Tigit-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg 2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc 2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct 2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag 2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg 2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag 2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt 2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt 2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg 2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg 2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct 2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg 2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc 2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga 3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct 3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca 3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg 3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg 3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat 3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg 3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa 3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca 3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatctatttc cggtgaattc 3541 ctcgagacta gttctagaGC CACCatgcat ggctggctgc tcctggtctg ggtccagggg 3601 ctgatacagg ctgccttcct cgctacagga gccacagcag gcacgataga tacaaagagg 3661 aacatctctg cagaggaagg tggctctgtc atcttacagt gtcacttctc ctctgacaca 3721 gctgaagtga cccaagtcga ctggaagcag caggaccagc ttctggccat ttatagtgtt 3781 gacctggggt ggcatgtcgc ttcagtcttc agtgatcggg tggtcccagg ccccagccta 3841 ggcctcacct tccagtctct gacaatgaat gacacgggag agtacttctg tacctatcat 3901 acgtatcctg gtgggattta caaggggaga atattcctga aggtccaaga aagctcagtg 3961 gctcagttcc agactgcccc gcttggagga accatggctg ctgtgctggg actcatttgc 4021 ttaatggtca caggagtgac tgtactggct agaaagaagt ctattagaat gcattctata 4081 gaaagtggcc ttgggagaac agaagcggag ccacaggaat ggaacctgag gagtctctca 4141 tcccctggaa gccctgtcca gacacaaact gcccctgctg gtccctgtgg agagcaggca 4201 gaagatgact atgctgaccc acaggaatac tttaatgtcc tgagctacag aagcctagag 4261 agcttcattg ctgtatcgaa gactggctaa gcggccgcgg atcccgcccc tctccctccc 4321 ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata 4381 tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg 4441 tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt 4501 tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag 4561 cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc 4621 cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga 4681 tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg 4741 cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat 4801 gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc 4861 tttgaaaaac acgatgataa gcttgccaca acccacaagg agacgacctt ccatgaccga 4921 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg tacgcaccct 4981 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgacccgg accgccacat 5041 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa 5101 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga 5161 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct 5221 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg 5281 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt 5341 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc 5401 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga 5461 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct agacgcgtct 5521 ggaacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg 5581 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt 5641 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg 5701 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc 5761 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc 5821 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc 5881 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc 5941 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg 6001 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc 6061 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa 6121 ttaattctgc agtcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc 6181 taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt 6241 cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt 6301 tttaaaagaa aagaggggac tggaagggct aattcactcc caacgaagac aagatatcct 6361 tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg 6421 gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc 6481 agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct 6541 gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc 6601 atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc 6661 ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg 6721 ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc 6781 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 6841 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 6901 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta 6961 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg 7021 agaggccttg acattgctag cgtttaccgt cgacctctag ctagagcttg gcgtaatcat 7081 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag 7141 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg 7201 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa 7261 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca 7321 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 7381 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 7441 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 7501 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 7561 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 7621 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 7681 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 7741 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 7801 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 7861 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 7921 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 7981 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 8041 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 8101 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 8161 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 8221 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 8281 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 8341 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 8401 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 8461 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 8521 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 8581 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 8641 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 8701 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 8761 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 8821 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac 8881 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 8941 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 9001 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 9061 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 9121 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 9181 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg 9241 acggatcggg agatcaactt gtttattgca gcttataatg gttacaaata aagcaatagc 9301 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 9361 ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat 9421 cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc 9481 taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt 9541 actacaaact tagtagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.