pEF1a-G-CEPIA1er plasmid

pEF1a-G-CEPIA1er plasmid

PVT94493

pEF1a-G-CEPIA1er plasmid Information

Plasmid name: pEF1a-G-CEPIA1er
Alias:
Gene lenght: 1392bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species:
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: EF1a
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: BGH-R
Copy number: High
Induction:

pEF1a-G-CEPIA1er plasmid Description

pEF1a-G-CEPIA1er is a plasmid suitable for protein expression in mammalian cells.

pEF1a-G-CEPIA1er plasmid Sequence

1 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 61 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 121 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 181 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 241 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgta tgcacgaacc 301 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 361 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 421 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac 481 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 541 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 601 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 661 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 721 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 781 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 841 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 901 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 961 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 1021 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 1081 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 1141 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 1201 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 1261 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 1321 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 1381 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 1441 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 1501 accgctgttg agatccagtt cgatgtaacc cactcgggca cccaactgat cttcagcatc 1561 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 1621 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 1681 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 1741 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcagatcgac 1801 ggatcgggag atcggtaccg aattcGGCTC CGGTGCCCGT CAGTGGGCAG AGCGCACATC 1861 GCCCACAGTC CCCGAGAAGT TGGGGGGAGG GGTCGGCAAT TGAACCGGTG CCTAGAGAAG 1921 GTGGCGCGGG GTAAACTGGG AAAGTGATGT CGTGTACTGG CTCCGCCTTT TTCCCGAGGG 1981 TGGGGGAGAA CCGTATATAA GTGCAGTAGT CGCCGTGAAC GTTCTTTTTC GCAACGGGTT 2041 TGCCGCCAGA ACACAGGTAA GTGCCGTGTG TGGTTCCCGC GGGCCTGGCC TCTTTACGGG 2101 TTATGGCCCT TGCGTGCCTT GAATTACTTC CACCTGGCTG CAGTACGTGA TTCTTGATCC 2161 CGAGCTTCGG GTTGGAAGTG GGTGGGAGAG TTCGAGGCCT TGCGCTTAAG GAGCCCCTTC 2221 GCCTCGTGCT TGAGTTGAGG CCTGGCCTGG GCGCTGGGGC CGCCGCGTGC GAATCTGGTG 2281 GCACCTTCGC GCCTGTCTCG CTGCTTTCGA TAAGTCTCTA GCCATTTAAA ATTTTTGATG 2341 ACCTGCTGCG ACGCTTTTTT TCTGGCAAGA TAGTCTTGTA AATGCGGGCC AAGATCTGCA 2401 CACTGGTATT TCGGTTTTTG GGGCCGCGGG CGGCGACGGG GCCCGTGCGT CCCAGCGCAC 2461 ATGTTCGGCG AGGCGGGGCC TGCGAGCGCG GCCACCGAGA ATCGGACGGG GGTAGTCTCA 2521 AGCTGGCCGG CCTGCTCTGG TGCCTGGCCT CGCGCCGCCG TGTATCGCCC CGCCCTGGGC 2581 GGCAAGGCTG GCCCGGTCGG CACCAGTTGC GTGAGCGGAA AGATGGCCGC TTCCCGGCCC 2641 TGCTGCAGGG AGCTCAAAAT GGAGGACGCG GCGCTCGGGA GAGCGGGCGG GTGAGTCACC 2701 CACACAAAGG AAAAGGGCCT TTCCGTCCTC AGCCGTCGCT TCATGTGACT CCACGGAGTA 2761 CCGGGCGCCG TCCAGGCACC TCGATTAGTT CTCGAGCTTT TGGAGTACGT CGTCTTTAGG 2821 TTGGGGGGAG GGGTTTTATG CGATGGAGTT TCCCCACACT GAGTGGGTGG AGACTGAAGT 2881 TAGGCCAGCT TGGCACTTGA TGTAATTCTC CTTGGAATTT GCCCTTTTTG AGTTTGGATC 2941 TTGGTTCATT CTCAAGCCTC AGACAGTGGT TCAAAGTTTT TTTCTTCCAT TTCAGGTGTC 3001 GTGAcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca 3061 tagaagacac cgactctact agaggatcgc tagcgctacc ggactcagat ctcgagGCCA 3121 CCATGGGATG GAGCTGTATC ATCCTCTTCT TGGTAGCAAC AGCTACAGGT AAGGGGTTAA 3181 CAGTAGCAGG CTTGAGGTCT GGACATATAT ATGGGTGACA ATGACATCCA CTTTGCCTTT 3241 CTCTCCACAG GCGCGCACTC CCAGGTCCAA CTGCAGGGAT CCATGGTCGA CTCTTCACGT 3301 CGTAAGTGGA ATAAGACAGG TCACGCAGTC AGAGCTATAG GTCGGCTGAG CTCACTCGAG 3361 AACGTCTATA TCAAGGCCGA CGAGCAGAAG AACGGCATCA AGGCGTACTT CAAGATCCGC 3421 CACAACATCG AGGGCGGCGG CGTGCAGCTC GCCTACCACT ACCAGCAGAA CACCCCCATC 3481 GGCGACGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA GCGTGCAGTC CATACTTTCG 3541 AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG 3601 ATCACTCTCG GCATGGACGA GCTGTACAAG GGCGGTACCG GAGGGAGCAT GGTGAGCAAG 3661 GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCAGGTCG AGCTGGACGG CGACGTAAAC 3721 GGCCACAAGT TCAGCGTGTC CGGCGAGGGT GAGGGCGATG CCACCTACGG CAAGCTGACC 3781 CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC 3841 CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC 3901 TTCAAGTCCG CCATGCCCGA AGGCTACATC CAGGAGCGCA CCATCTTCTT CAAGGACGAC 3961 GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC 4021 GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC 4081 AACACGCGTG ACCAACTGAC TGAAGAGCAG ATCGCAGAAT TTAAAGAGGC TTTCTCCCTA 4141 TTTGACAAGG ACGGGGATGG GACGATAACA ACCAAGGATC TGGGGACGGT GCTGCGGTCT 4201 CTGGGGCAGA ACCCCACAGA AGCAGAGCTC CAGGACATGA TCAATGAAGT AGATGCCGAC 4261 GGTAATGGCA CAATCGACTT CCCTGAGTTC CTGACAATGA TGGCAAGAAA AATGAAAGAC 4321 ACAGACAGTG AAGAAGAAAT TCGCGAAGCG TTCCGTGTGT GGGATAAGGA TGGCAATGGC 4381 TACATCTCTG CAGCAGACCT TCGCCACGTG ATGACAAACC TTGGAGAGAA GTTAACAGAT 4441 GAAGAGGTTG ATGAAATGAT CAGGGAAGCA GATATCGATG GAGAAGGTCA GGTAAACTAC 4501 GAAGAGTTTG TACAAATGAT GACAGCGAAG GCGGCCGCAG AACAAAAACT CATCTCAGAA 4561 GAGGATCTGA ATGGGGCCGC AAGCGAGAAG GACGAGCTGT AGtctagaag ctcgctgatc 4621 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc 4681 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc 4741 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg 4801 ggaggattgg gaagacaata gcaggcatgc tggggatggc ccgggctcta tggcttctga 4861 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt 4921 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc 4981 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca 5041 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctctcccc 5101 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt 5161 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac 5221 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc 5281 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat 5341 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc 5401 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga 5461 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc 5521 atcccgcccc taactccgcc cagttccgcc cattctccgc ccctaggctg actaattttt 5581 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga 5641 ggcttttttg gaggcctagg cttttgcaaa aagctccccc cccgggaggt ccacaatggt 5701 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta 5761 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca 5821 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactccagga 5881 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga 5941 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct 6001 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg 6061 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga 6121 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca 6181 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgtatgc ccgacggcga 6241 ggatctcgtc gtgactcatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg 6301 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc 6361 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt 6421 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga 6481 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca 6541 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc 6601 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac 6661 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 6721 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 6781 tcttatcatg tctgtatacc gtcgatcttt ccgcttcctc gctcactgac tcgctgcgct 6841 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 6901 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aa

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.