pLV3-CMV-UBE2C-3×FLAG-CopGFP-P

pLV3-CMV-UBE2C-3×FLAG-CopGFP-Puro plasmid

PVT96564

pLV3-CMV-UBE2C-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-UBE2C(human)-3×FLAG-CopGFP-Puro
Alias: NM_007019.4;dJ447F3.2; UBCH10
Gene lenght: 537bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-UBE2C-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-UBE2C-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-UBE2C-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg cttcccaaaa ccgcgaccca 2341 gccgccacta gcgtcgccgc cgcccgtaaa ggagctgagc cgagcggggg cgccgcccgg 2401 ggtccggtgg gcaaaaggct acagcaggag ctgatgaccc tcatgatgtc tggcgataaa 2461 gggatttctg ccttccctga atcagacaac cttttcaaat gggtagggac catccatgga 2521 gcagctggaa cagtatatga agacctgagg tataagctct cgctagagtt ccccagtggc 2581 tacccttaca atgcgcccac agtgaagttc ctcacgccct gctatcaccc caacgtggac 2641 acccagggta acatatgcct ggacatcctg aaggaaaagt ggtctgccct gtatgatgtc 2701 aggaccattc tgctctccat ccagagcctt ctaggagaac ccaacattga tagtcccttg 2761 aacacacatg ctgccgagct ctggaaaaac cccacagctt ttaagaagta cctgcaagaa 2821 acctactcaa agcaggtcac cagccaggag cccggatccG ACTACAAAGA CCATGACGGT 2881 GATTATAAAG ATCATGACAT CGACTACAAG GATGACGATG ACAAGtagcg gccgcGaagg 2941 atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag 3001 aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac 3061 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat 3121 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag 3181 ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc 3241 catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg 3301 tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct 3361 tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc 3421 tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct 3481 acgctagacg ccaccatgga gagcgacgag agcggcctgc ccgccatgga gatcgagtgc 3541 cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg agagggcacc 3601 cccaagcagg gccgcatgac caacaagatg aagagcacca aaggcgccct gaccttcagc 3661 ccctacctgc tgagccacgt gatgggctac ggcttctacc acttcggcac ctaccccagc 3721 ggctacgaga accccttcct gcacgccatc aacaacggcg gctacaccaa cacccgcatc 3781 gagaagtacg aggacggcgg cgtgctgcac gtgagcttca gctaccgcta cgaggccggc 3841 cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc ccgaggacag cgtgatcttc 3901 accgacaaga tcatccgcag caacgccacc gtggagcacc tgcaccccat gggcgataac 3961 gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta ctacagcttc 4021 gtggtggaca gccacatgca cttcaagagc gccatccacc ccagcatcct gcagaacggg 4081 ggccccatgt tcgccttccg ccgcgtggag gagctgcaca gcaacaccga gctgggcatc 4141 gtggagtacc agcacgcctt caagaccccc atcgccttcg ccagatcccg cgctcagtcg 4201 tccaattctg ccgtggacgg caccgccgga cccggctcca ccggatctcg cgagggcaga 4261 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctatgac cgagtacaag 4321 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc 4381 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg 4441 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg 4501 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg 4561 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg 4621 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg 4681 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc 4741 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc 4801 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc 4861 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaaatca acctctggat 4921 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt 4981 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc 5041 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg 5101 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc 5161 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa 5221 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat 5281 tccgtggtgt tgtcggggaa ATCATCGTCC TTTCCTtggc tgctcgcctg tgttgccacc 5341 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt 5401 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtctTcgcct tcgccctcag 5461 acgagtcgga tctccctttg gGccgcctcc ccgcctggta cctttaagac caatgactta 5521 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat 5581 tcactcccaa cgaaGataag atctgctttt tgcttgtact gggtctctct ggttagacca 5641 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag 5701 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag 5761 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct 5821 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt 5881 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc 5941 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt 6001 ctggctctag ctaTCCCGCC CCTAACTCCG CCCAtcccgc ccctaactcc gcccagttcc 6061 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc 6121 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc 6181 agagaccaaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac 6241 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 6301 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 6361 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 6421 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 6481 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 6541 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 6601 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 6661 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 6721 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 6781 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 6841 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 6901 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 6961 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 7021 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 7081 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 7141 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 7201 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 7261 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 7321 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 7381 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 7441 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 7501 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 7561 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 7621 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 7681 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 7741 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 7801 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 7861 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 7921 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 7981 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 8041 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 8101 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 8161 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 8221 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 8281 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 8341 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag 8401 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca 8461 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc 8521 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc 8581 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag 8641 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg 8701 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg 8761 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc 8821 caagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.