pEF1a-CMV-CXCL10-Neo plasmid

pEF1a-CMV-CXCL10-Neo plasmid

PVT96560

pEF1a-CMV-CXCL10-Neo plasmid Information

Plasmid name: pEF1a-CMV-CXCL10(human)-Neo
Alias: NM_001565.4;C7; crg-2; gIP-10; IFI10; INP10; IP-10;mob-1; SCYB10
Gene lenght: 297bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 30°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pEF1a-CMV-CXCL10-Neo plasmid Description

pEF1a-CMV-CXCL10-Neo is a plasmid suitable for protein expression in mammalian cells.

pEF1a-CMV-CXCL10-Neo plasmid Sequence

1 CGCGTTGACA TTGATTATTG ACTAGGCTTT TGCAAAAAGC TTTGCAAAGA TGGATAAAGT 61 TTTAAACAGA GAGGAATCTT TGCAGCTAAT GGACCTTCTA GGTCTTGAAA GGAGTGGGAA 121 TTGGCTCCGG TGCCCGTCAG TGGGCAGAGC GCACATCGCC CACAGTCCCC GAGAAGTTGG 181 GGGGAGGGGT CGGCAATTGA ACCGGTGCCT AGAGAAGGTG GCGCGGGGTA AACTGGGAAA 241 GTGATGTCGT GTACTGGCTC CGCCTTTTTC CCGAGGGTGG GGGAGAACCG TATATAAGTG 301 CAGTAGTCGC CGTGAACGTT CTTTTTCGCA ACGGGTTTGC CGCCAGAACA CAGGTAAGTG 361 CCGTGTGTGG TTCCCGCGGG CCTGGCCTCT TTACGGGTTA TGGCCCTTGC GTGCCTTGAA 421 TTACTTCCAC CTGGCTGCAG TACGTGATTC TTGATCCCGA GCTTCGGGTT GGAAGTGGGT 481 GGGAGAGTTC GAGGCCTTGC GCTTAAGGAG CCCCTTCGCC TCGTGCTTGA GTTGAGGCCT 541 GGCCTGGGCG CTGGGGCCGC CGCGTGCGAA TCTGGTGGCA CCTTCGCGCC TGTCTCGCTG 601 CTTTCGATAA GTCTCTAGCC ATTTAAAATT TTTGATGACC TGCTGCGACG CTTTTTTTCT 661 GGCAAGATAG TCTTGTAAAT GCGGGCCAAG ATCTGCACAC TGGTATTTCG GTTTTTGGGG 721 CCGCGGGCGG CGACGGGGCC CGTGCGTCCC AGCGCACATG TTCGGCGAGG CGGGGCCTGC 781 GAGCGCGGCC ACCGAGAATC GGACGGGGGT AGTCTCAAGC TGGCCGGCCT GCTCTGGTGC 841 CTGGCCTCGC GCCGCCGTGT ATCGCCCCGC CCTGGGCGGC AAGGCTGGCC CGGTCGGCAC 901 CAGTTGCGTG AGCGGAAAGA TGGCCGCTTC CCGGCCCTGC TGCAGGGAGC TCAAAATGGA 961 GGACGCGGCG CTCGGGAGAG CGGGCGGGTG AGTCACCCAC ACAAAGGAAA AGGGCCTTTC 1021 CGTCCTCAGC CGTCGCTTCA TGTGACTCCA CGGAGTACCG GGCGCCGTCC AGGCACCTCG 1081 ATTAGTTCTC GAGCTTTTGG AGTACGTCGT CTTTAGGTTG GGGGGAGGGG TTTTATGCGA 1141 TGGAGTTTCC CCACACTGAG TGGGTGGAGA CTGAAGTTAG GCCAGCTTGG CACTTGATGT 1201 AATTCTCCTT GGAATTTGCC CTTTTTGAGT TTGGATCTTG GTTCATTCTC AAGCCTCAGA 1261 CAGTGGTTCA AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AGGAATTAGC TTGGTACGCT 1321 AGCGTTTAAA CTTAAGCTTG GTACCgaagt gctccggggt gatagtgttg agaagacctc 1381 tcgggtatgg tcaggtaatg aacgtgacca ggagctgctt actgaggacg cactggatga 1441 tctcatccct tcttttctac tgactggtca acagacaccg gcgttcggtc gaagagtatc 1501 tggtgtcata gaaattgccg atgggagtcg ccgtcgtaaa gctgctgcac ttaccgaaag 1561 tgattatcgt gttctggttg gcgagctgga tgatgagcag atggctgcat tatccagatt 1621 gggtaacgat tatcgcccaa caagtgctta tgaacgtggt cagcgttatg caagccgatt 1681 gcagaatgaa tttgctggaa atatttctgc gctggctgat gcggaaaata tttcacgtaa 1741 gattattacc cgctgtatca acaccgccaa attgcctaaa tcagttgttg ctcttttttc 1801 tcaccccggt gaactatctg cccggtcagg tgatgcactt caaaaagcct ttacagataa 1861 agaggaatta cttaagcagc aggcatctaa ccttcatgag cagaaaaaag ctggggtgat 1921 atttgaagct gaagaagtta tcactctttt aacttctgtg cttaaaacgt catctgcatc 1981 aagaactagt ttaagctcac gacatcagtt tgctcctgga gcgacagtat tgtataaggg 2041 cgataaaatg gtgcttaacc tggacaggtc tcgtgttcca actgagtgta tagagaaaat 2101 tgaggccatt cttaaggaac ttgaaaagcc agcaccctga tgcgaccacg ttttagtcta 2161 cgtttatctg tctttactta atgtcctttg ttacaggcca gaaagcataa ctggcctgaa 2221 tattctctct gggcccactg ttccacttgt atcgtcggtc tgataatcag actgggacca 2281 cggtcccact cgtatcgtcg gtctgattat tagtctggga ccacggtccc actcgtatcg 2341 tcggtctgat tattagtctg ggaccacggt cccactcgta tcgtcggtct gataatcaga 2401 ctgggaccac ggtcccactc gtatcgtcgg tctgattatt agtctgggac catggtccca 2461 ctcgtatcgt cggtctgatt attagtctgg gaccacggtc ccactcgtat cgtcggtctg 2521 attattagtc tggaaccacg gtcccactcg tatcgtcggt ctgattatta gtctgggacc 2581 acggtcccac tcgtatcgtc ggtctgatta ttagtctggg accacgatcc cactcgtgtt 2641 gtcggtctga ttatcggtct gggaccacgg tcccacttgt attgtcgatc agactatcag 2701 cgtgagacta cgattccatc aatgcctgtc aagggcaagt attgacatgt cgtcgtaacc 2761 tgtagaacgg agtaacctcg gtgtgcggtt gtatgcctgc tgtggattgc tgctgtgtcc 2821 tgcttatcca caacattttg cgcacggtta tgtggacaaa atacctggtt acccaggccg 2881 tgccggcacg ttccaagacg tccaaagccc aggcaaacac gttatacagg tcgccgttgg 2941 gggccagcaa ctcgggggcc cgaaacaggg taaataacgt gtccccgata tggggtcgtg 3001 ggcccgcgtt gctctggggc tcggcaccct ggggcggcac ggccgccccc gaaagctgtc 3061 cccaatcctc ccgccacgac ccgccgccct gcagataccg caccgGACAT TGATTATTGA 3121 CTAGTTATTA ATAGTAATCA ATTACGGGGT CATTAGTTCA TAGCCCATAT ATGGAGTTCC 3181 GCGTTACATA ACTTACGGTA AATGGCCCGC CTGGCTGACC GCCCAACGAC CCCCGCCCAT 3241 TGACGTCAAT AATGACGTAT GTTCCCATAG TAACGCCAAT AGGGACTTTC CATTGACGTC 3301 AATGGGTGGA GTATTTACGG TAAACTGCCC ACTTGGCAGT ACATCAAGTG TATCATATGC 3361 CAAGTACGCC CCCTATTGAC GTCAATGACG GTAAATGGCC CGCCTGGCAT TATGCCCAGT 3421 ACATGACCTT ATGGGACTTT CCTACTTGGC AGTACATCTA CGTATTAGTC ATCGCTATTA 3481 CCATGGTGAT GCGGTTTTGG CAGTACATCA ATGGGCGTGG ATAGCGGTTT GACTCACGGG 3541 GATTTCCAAG TCTCCACCCC ATTGACGTCA ATGGGAGTTT GTTTTGGCAC CAAAATCAAC 3601 GGGACTTTCC AAAATGTCGT AACAACTCCG CCCCATTGAC GCAAATGGGC GGTAGGCGTG 3661 TACGGTGGGA GGTCTATATA AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT 3721 GGCTTATCGA AATTAATACG ACTCACTATA GGGAGACCCA AGCTGGCTAG Cgccaccatg 3781 aatcaaactg ccattctgat ttgctgcctt atctttctga ctctaagtgg cattcaagga 3841 gtacctctct ctagaactgt acgctgtacc tgcatcagca ttagtaatca acctgttaat 3901 ccaaggtctt tagaaaaact tgaaattatt cctgcaagcc aattttgtcc acgtgttgag 3961 atcattgcta caatgaaaaa gaagggtgag aagagatgtc tgaatccaga atcgaaggcc 4021 atcaagaatt tactgaaagc agttagcaag gaaaggtcta aaagatctcc ttaaaacttg 4081 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa 4141 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatatt 4201 ggcaagcagc ccataaacgc ggcgaatcgc ggccagcata gccaggtcaa gccgctcgcc 4261 ggggcgctgg cgtttggcca ggcggtcgat gtgtctgtcc tccggaaggg cccccaacac 4321 gatgtttgtg ccgggcaagg tcggcgggat gagggccacg aacgccagca cggcctgggg 4381 ggtcatgctg cccataaggt atcgcgcggc cgggtagcac aggagggcgg cgatgggatg 4441 gcggtcgaag atgagggtga gggccggggg cggggcatgt gagctcccag cctccccccc 4501 gatatgagga gccagaacgg cgtcggtcac ggcataaggc atgcccattg ttatctgggc 4561 gcttgtcatt accaccgccg cgtccccggc cgatatctca ccctggtcga ggcggtgttg 4621 tgtggtgtag atgttcgcga ttgtctcgga agcccccaac acccgccagt aagtcatcgg 4681 ctcgggtacg tagacgatat cgtcgcgcga acccagggcc accagcagtt gcgtggtggt 4741 ggttttcccc atcccgtggg gaccgtctat ataaacccgc agtagcgtgg gcattttctg 4801 ctccaggcgg acttccgtgg ctttttgctg ccggcgaggg cgcaacgccg tacgtcggtt 4861 gttatggccg cgagaacgcg cagcctggtc gaacgcagac gcgtgttgat ggcaggggta 4921 cgaagccata cgcgcttcta caaggcgctt gccgaagagg tgcgggagtt tcacgccacc 4981 aagatctgcg gcacgctgtt gacgctgtta agcgggtcgc tgcagggtcg ctcggtgttc 5041 gaggccacac gcgtcacctt aatatgcgaa gtggacctgg gaccgcgccg ccccgactgc 5101 atctgcgtgt tcgaattcgc caatgacaag acgctgggcg gggtttgtgt catcatagaa 5161 ctaaagacat gcaaatatat ttcttccggg gacaccgcca gcaaacgcga gcaacgggcc 5221 acggggatga agcagctgcg ccactccctg aagctcctgc agtccctcgc gcctccgggt 5281 gacaagatag tgtacctgtg ccccgtcctg gtgtttgtcg cccaacggac gctccgcgtc 5341 agccgcgtga cccggctcgt cccgcagaag gtctccggta atatcaccgc agtcgtgcgg 5401 atgctccaga gcctgtccac gtatacggtc cccatggagc ctaggaccca gcgagcccgt 5461 cgccgccgcg gcggcgccgc ccgggggtct gcgagcagac cgaaaaggtc acactctggg 5521 gcgcgcgacc cgcccgagtc agcggcccgc cagttaccac ccgccgacca aacccccgcc 5581 tccacggagg gcgggggggt gcttaagagg atcgcggcgc tcttctgcgt gcccgtggcc 5641 accaagacca aaccccgagc cgcctccgaa tgagagtgtt tcgttccttc cccctccccc 5701 cgcgtcagac aaaccctaac caccgcttaa gcggcccccg cgaggtccga agactcattt 5761 ggatccggcg ggagccaccc gacaacagcc cccgggtttt cccacgccag acgccggtcc 5821 gctgtgccat cgcgccccct catcccaccc cccatcttgt ccccaaataa aacaaggtct 5881 ggtagttagg acaacgaccg cagttctcgt gtgttatttt cgctctccgc ctctcgcaga 5941 tggacccgta ctgcccGGAT CCtcagacGC GGCCGCGACT CTAGATCATA ATCAGCCATA 6001 CCACATTTGT AGAGGTTTTA CTTGCTTTAA AAAACCTCCC ACACCTCCCC CTGAACCTGA 6061 AACATAAAAT GAATGCAATT GTTGTTGTTA ACTTGTTTAT TGCAGCTTAT AATGGTTACA 6121 AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT 6181 GTGGTTTGTC CAAACTCATC AATGTATCTT AAGGCGTAAA TTGTAAGCGT TAATATTTTG 6241 TTAAAATTCG CGTTAAATTT TTGTTAAATC AGCTCATTTT TTAACCAATA GGCCGAAATC 6301 GGCAAAATCC CTTATAAATC AAAAGAATAG ACCGAGATAG GGTTGAGTGT TGTTCCAGTT 6361 TGGAACAAGA GTCCACTATT AAAGAACGTG GACTCCAACG TCAAAGGGCG AAAAACCGTC 6421 TATCAGGGCG ATGGCCCACT ACGTGAACCA TCACCCTAAT CAAGTTTTTT GGGGTCGAGG 6481 TGCCGTAAAG CACTAAATCG GAACCCTAAA GGGAGCCCCC GATTTAGAGC TTGACGGGGA 6541 AAGCCGGCGA ACGTGGCGAG AAAGGAAGGG AAGAAAGCGA AAGGAGCGGG CGCTAGGGCG 6601 CTGGCAAGTG TAGCGGTCAC GCTGCGCGTA ACCACCACAC CCGCCGCGCT TAATGCGCCG 6661 CTACAGGGCG CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA 6721 TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT 6781 CAATAATATT GAAAAAGGAA GAGTCCTGAG GCGGAAAGAA CCAGCTGTGG AATGTGTGTC 6841 AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC 6901 TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC 6961 AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC 7021 CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT 7081 ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT 7141 TTGGAGGCCT AGGCTTTTGC AAAGATCGAT CAAGAGACAG GATGAGGATC GTTTCGCATG 7201 ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC 7261 TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG 7321 CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAA 7381 GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC 7441 GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT 7501 CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG 7561 CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC 7621 GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG 7681 CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT GCCCGACGGC 7741 GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC 7801 CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA 7861 GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC 7921 GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC 7981 GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC 8041 CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT 8101 TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC 8161 ACCCTAGGGG GAGGCTAACT GAAACACGGA AGGAGACAAT ACCGGAAGGA ACCCGCGCTA 8221 TGACGGCAAT AAAAAGACAG AATAAAACGC ACGGTGTTGG GTCGTTTGTT CATAAACGCG 8281 GGGTTCGGTC CCAGGGCTGG CACTCTGTCG ATACCCCACC GAGACCCCAT TGGGGCCAAT 8341 ACGCCCGCGT TTCTTCCTTT TCCCCACCCC ACCCCCCAAG TTCGGGTGAA GGCCCAGGGC 8401 TCGCAGCCAA CGTCGGGGCG GCAGGCCCTG CCATAGCCTC AGGTTACTCA TATATACTTT 8461 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 8521 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 8581 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 8641 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 8701 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC 8761 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 8821 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 8881 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 8941 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 9001 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 9061 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 9121 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 9181 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 9241 CTCACATGT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.