pEF1a-CMV-CXCL10-EGFP-Neo plas

pEF1a-CMV-CXCL10-EGFP-Neo plasmid

PVT96111

pEF1a-CMV-CXCL10-EGFP-Neo plasmid Information

Plasmid name: pEF1a-CMV-CXCL10(human)-EGFP-Neo
Alias: NM_001565.4;C7; crg-2; gIP-10; IFI10; INP10; IP-10;mob-1; SCYB10
Gene lenght: 297bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 30°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pEF1a-CMV-CXCL10-EGFP-Neo plasmid Description

pEF1a-CMV-CXCL10-EGFP-Neo is a plasmid suitable for protein expression in mammalian cells.

pEF1a-CMV-CXCL10-EGFP-Neo plasmid Sequence

1 CGCGTTGACA TTGATTATTG ACTAGGCTTT TGCAAAAAGC TTTGCAAAGA TGGATAAAGT 61 TTTAAACAGA GAGGAATCTT TGCAGCTAAT GGACCTTCTA GGTCTTGAAA GGAGTGGGAA 121 TTGGCTCCGG TGCCCGTCAG TGGGCAGAGC GCACATCGCC CACAGTCCCC GAGAAGTTGG 181 GGGGAGGGGT CGGCAATTGA ACCGGTGCCT AGAGAAGGTG GCGCGGGGTA AACTGGGAAA 241 GTGATGTCGT GTACTGGCTC CGCCTTTTTC CCGAGGGTGG GGGAGAACCG TATATAAGTG 301 CAGTAGTCGC CGTGAACGTT CTTTTTCGCA ACGGGTTTGC CGCCAGAACA CAGGTAAGTG 361 CCGTGTGTGG TTCCCGCGGG CCTGGCCTCT TTACGGGTTA TGGCCCTTGC GTGCCTTGAA 421 TTACTTCCAC CTGGCTGCAG TACGTGATTC TTGATCCCGA GCTTCGGGTT GGAAGTGGGT 481 GGGAGAGTTC GAGGCCTTGC GCTTAAGGAG CCCCTTCGCC TCGTGCTTGA GTTGAGGCCT 541 GGCCTGGGCG CTGGGGCCGC CGCGTGCGAA TCTGGTGGCA CCTTCGCGCC TGTCTCGCTG 601 CTTTCGATAA GTCTCTAGCC ATTTAAAATT TTTGATGACC TGCTGCGACG CTTTTTTTCT 661 GGCAAGATAG TCTTGTAAAT GCGGGCCAAG ATCTGCACAC TGGTATTTCG GTTTTTGGGG 721 CCGCGGGCGG CGACGGGGCC CGTGCGTCCC AGCGCACATG TTCGGCGAGG CGGGGCCTGC 781 GAGCGCGGCC ACCGAGAATC GGACGGGGGT AGTCTCAAGC TGGCCGGCCT GCTCTGGTGC 841 CTGGCCTCGC GCCGCCGTGT ATCGCCCCGC CCTGGGCGGC AAGGCTGGCC CGGTCGGCAC 901 CAGTTGCGTG AGCGGAAAGA TGGCCGCTTC CCGGCCCTGC TGCAGGGAGC TCAAAATGGA 961 GGACGCGGCG CTCGGGAGAG CGGGCGGGTG AGTCACCCAC ACAAAGGAAA AGGGCCTTTC 1021 CGTCCTCAGC CGTCGCTTCA TGTGACTCCA CGGAGTACCG GGCGCCGTCC AGGCACCTCG 1081 ATTAGTTCTC GAGCTTTTGG AGTACGTCGT CTTTAGGTTG GGGGGAGGGG TTTTATGCGA 1141 TGGAGTTTCC CCACACTGAG TGGGTGGAGA CTGAAGTTAG GCCAGCTTGG CACTTGATGT 1201 AATTCTCCTT GGAATTTGCC CTTTTTGAGT TTGGATCTTG GTTCATTCTC AAGCCTCAGA 1261 CAGTGGTTCA AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AGGAATTAGC TTGGTACGCT 1321 AGCGTTTAAA CTTAAGCTTG GTACCgaagt gctccggggt gatagtgttg agaagacctc 1381 tcgggtatgg tcaggtaatg aacgtgacca ggagctgctt actgaggacg cactggatga 1441 tctcatccct tcttttctac tgactggtca acagacaccg gcgttcggtc gaagagtatc 1501 tggtgtcata gaaattgccg atgggagtcg ccgtcgtaaa gctgctgcac ttaccgaaag 1561 tgattatcgt gttctggttg gcgagctgga tgatgagcag atggctgcat tatccagatt 1621 gggtaacgat tatcgcccaa caagtgctta tgaacgtggt cagcgttatg caagccgatt 1681 gcagaatgaa tttgctggaa atatttctgc gctggctgat gcggaaaata tttcacgtaa 1741 gattattacc cgctgtatca acaccgccaa attgcctaaa tcagttgttg ctcttttttc 1801 tcaccccggt gaactatctg cccggtcagg tgatgcactt caaaaagcct ttacagataa 1861 agaggaatta cttaagcagc aggcatctaa ccttcatgag cagaaaaaag ctggggtgat 1921 atttgaagct gaagaagtta tcactctttt aacttctgtg cttaaaacgt catctgcatc 1981 aagaactagt ttaagctcac gacatcagtt tgctcctgga gcgacagtat tgtataaggg 2041 cgataaaatg gtgcttaacc tggacaggtc tcgtgttcca actgagtgta tagagaaaat 2101 tgaggccatt cttaaggaac ttgaaaagcc agcaccctga tgcgaccacg ttttagtcta 2161 cgtttatctg tctttactta atgtcctttg ttacaggcca gaaagcataa ctggcctgaa 2221 tattctctct gggcccactg ttccacttgt atcgtcggtc tgataatcag actgggacca 2281 cggtcccact cgtatcgtcg gtctgattat tagtctggga ccacggtccc actcgtatcg 2341 tcggtctgat tattagtctg ggaccacggt cccactcgta tcgtcggtct gataatcaga 2401 ctgggaccac ggtcccactc gtatcgtcgg tctgattatt agtctgggac catggtccca 2461 ctcgtatcgt cggtctgatt attagtctgg gaccacggtc ccactcgtat cgtcggtctg 2521 attattagtc tggaaccacg gtcccactcg tatcgtcggt ctgattatta gtctgggacc 2581 acggtcccac tcgtatcgtc ggtctgatta ttagtctggg accacgatcc cactcgtgtt 2641 gtcggtctga ttatcggtct gggaccacgg tcccacttgt attgtcgatc agactatcag 2701 cgtgagacta cgattccatc aatgcctgtc aagggcaagt attgacatgt cgtcgtaacc 2761 tgtagaacgg agtaacctcg gtgtgcggtt gtatgcctgc tgtggattgc tgctgtgtcc 2821 tgcttatcca caacattttg cgcacggtta tgtggacaaa atacctggtt acccaggccg 2881 tgccggcacg ttccaagacg tccaaagccc aggcaaacac gttatacagg tcgccgttgg 2941 gggccagcaa ctcgggggcc cgaaacaggg taaataacgt gtccccgata tggggtcgtg 3001 ggcccgcgtt gctctggggc tcggcaccct ggggcggcac ggccgccccc gaaagctgtc 3061 cccaatcctc ccgccacgac ccgccgccct gcagataccg caccgGACAT TGATTATTGA 3121 CTAGTTATTA ATAGTAATCA ATTACGGGGT CATTAGTTCA TAGCCCATAT ATGGAGTTCC 3181 GCGTTACATA ACTTACGGTA AATGGCCCGC CTGGCTGACC GCCCAACGAC CCCCGCCCAT 3241 TGACGTCAAT AATGACGTAT GTTCCCATAG TAACGCCAAT AGGGACTTTC CATTGACGTC 3301 AATGGGTGGA GTATTTACGG TAAACTGCCC ACTTGGCAGT ACATCAAGTG TATCATATGC 3361 CAAGTACGCC CCCTATTGAC GTCAATGACG GTAAATGGCC CGCCTGGCAT TATGCCCAGT 3421 ACATGACCTT ATGGGACTTT CCTACTTGGC AGTACATCTA CGTATTAGTC ATCGCTATTA 3481 CCATGGTGAT GCGGTTTTGG CAGTACATCA ATGGGCGTGG ATAGCGGTTT GACTCACGGG 3541 GATTTCCAAG TCTCCACCCC ATTGACGTCA ATGGGAGTTT GTTTTGGCAC CAAAATCAAC 3601 GGGACTTTCC AAAATGTCGT AACAACTCCG CCCCATTGAC GCAAATGGGC GGTAGGCGTG 3661 TACGGTGGGA GGTCTATATA AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT 3721 GGCTTATCGA AATTAATACG ACTCACTATA GGGAGACCCA AGCTGGCTAG Cgccaccatg 3781 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc 3841 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc 3901 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc 3961 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag 4021 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc 4081 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg 4141 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag 4201 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc 4261 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac 4321 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac 4381 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg 4441 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caaggGaGGC 4501 GGAGGTAGTG GAGGAGGGGG AAGCggaggc ggagggagtG AATTCatgaa tcaaactgcc 4561 attctgattt gctgccttat ctttctgact ctaagtggca ttcaaggagt acctctctct 4621 agaactgtac gctgtacctg catcagcatt agtaatcaac ctgttaatcc aaggtcttta 4681 gaaaaacttg aaattattcc tgcaagccaa ttttgtccac gtgttgagat cattgctaca 4741 atgaaaaaga agggtgagaa gagatgtctg aatccagaat cgaaggccat caagaattta 4801 ctgaaagcag ttagcaagga aaggtctaaa agatctcctt aaaacttgtt tattgcagct 4861 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca 4921 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatattgg caagcagccc 4981 ataaacgcgg cgaatcgcgg ccagcatagc caggtcaagc cgctcgccgg ggcgctggcg 5041 tttggccagg cggtcgatgt gtctgtcctc cggaagggcc cccaacacga tgtttgtgcc 5101 gggcaaggtc ggcgggatga gggccacgaa cgccagcacg gcctgggggg tcatgctgcc 5161 cataaggtat cgcgcggccg ggtagcacag gagggcggcg atgggatggc ggtcgaagat 5221 gagggtgagg gccgggggcg gggcatgtga gctcccagcc tcccccccga tatgaggagc 5281 cagaacggcg tcggtcacgg cataaggcat gcccattgtt atctgggcgc ttgtcattac 5341 caccgccgcg tccccggccg atatctcacc ctggtcgagg cggtgttgtg tggtgtagat 5401 gttcgcgatt gtctcggaag cccccaacac ccgccagtaa gtcatcggct cgggtacgta 5461 gacgatatcg tcgcgcgaac ccagggccac cagcagttgc gtggtggtgg ttttccccat 5521 cccgtgggga ccgtctatat aaacccgcag tagcgtgggc attttctgct ccaggcggac 5581 ttccgtggct ttttgctgcc ggcgagggcg caacgccgta cgtcggttgt tatggccgcg 5641 agaacgcgca gcctggtcga acgcagacgc gtgttgatgg caggggtacg aagccatacg 5701 cgcttctaca aggcgcttgc cgaagaggtg cgggagtttc acgccaccaa gatctgcggc 5761 acgctgttga cgctgttaag cgggtcgctg cagggtcgct cggtgttcga ggccacacgc 5821 gtcaccttaa tatgcgaagt ggacctggga ccgcgccgcc ccgactgcat ctgcgtgttc 5881 gaattcgcca atgacaagac gctgggcggg gtttgtgtca tcatagaact aaagacatgc 5941 aaatatattt cttccgggga caccgccagc aaacgcgagc aacgggccac ggggatgaag 6001 cagctgcgcc actccctgaa gctcctgcag tccctcgcgc ctccgggtga caagatagtg 6061 tacctgtgcc ccgtcctggt gtttgtcgcc caacggacgc tccgcgtcag ccgcgtgacc 6121 cggctcgtcc cgcagaaggt ctccggtaat atcaccgcag tcgtgcggat gctccagagc 6181 ctgtccacgt atacggtccc catggagcct aggacccagc gagcccgtcg ccgccgcggc 6241 ggcgccgccc gggggtctgc gagcagaccg aaaaggtcac actctggggc gcgcgacccg 6301 cccgagtcag cggcccgcca gttaccaccc gccgaccaaa cccccgcctc cacggagggc 6361 gggggggtgc ttaagaggat cgcggcgctc ttctgcgtgc ccgtggccac caagaccaaa 6421 ccccgagccg cctccgaatg agagtgtttc gttccttccc cctccccccg cgtcagacaa 6481 accctaacca ccgcttaagc ggcccccgcg aggtccgaag actcatttgg atccggcggg 6541 agccacccga caacagcccc cgggttttcc cacgccagac gccggtccgc tgtgccatcg 6601 cgccccctca tcccaccccc catcttgtcc ccaaataaaa caaggtctgg tagttaggac 6661 aacgaccgca gttctcgtgt gttattttcg ctctccgcct ctcgcagatg gacccgtact 6721 gcccGGATCC tcagacGCGG CCGCGACTCT AGATCATAAT CAGCCATACC ACATTTGTAG 6781 AGGTTTTACT TGCTTTAAAA AACCTCCCAC ACCTCCCCCT GAACCTGAAA CATAAAATGA 6841 ATGCAATTGT TGTTGTTAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA 6901 GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA 6961 AACTCATCAA TGTATCTTAA GGCGTAAATT GTAAGCGTTA ATATTTTGTT AAAATTCGCG 7021 TTAAATTTTT GTTAAATCAG CTCATTTTTT AACCAATAGG CCGAAATCGG CAAAATCCCT 7081 TATAAATCAA AAGAATAGAC CGAGATAGGG TTGAGTGTTG TTCCAGTTTG GAACAAGAGT 7141 CCACTATTAA AGAACGTGGA CTCCAACGTC AAAGGGCGAA AAACCGTCTA TCAGGGCGAT 7201 GGCCCACTAC GTGAACCATC ACCCTAATCA AGTTTTTTGG GGTCGAGGTG CCGTAAAGCA 7261 CTAAATCGGA ACCCTAAAGG GAGCCCCCGA TTTAGAGCTT GACGGGGAAA GCCGGCGAAC 7321 GTGGCGAGAA AGGAAGGGAA GAAAGCGAAA GGAGCGGGCG CTAGGGCGCT GGCAAGTGTA 7381 GCGGTCACGC TGCGCGTAAC CACCACACCC GCCGCGCTTA ATGCGCCGCT ACAGGGCGCG 7441 TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA 7501 CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA 7561 AAAAGGAAGA GTCCTGAGGC GGAAAGAACC AGCTGTGGAA TGTGTGTCAG TTAGGGTGTG 7621 GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG 7681 CAACCAGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC 7741 TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC 7801 CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG 7861 AGGCCGCCTC GGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG 7921 GCTTTTGCAA AGATCGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT 7981 GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA 8041 CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG 8101 GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAAGA CGAGGCAGCG 8161 CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT 8221 GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT 8281 CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG 8341 CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT 8401 ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC 8461 GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGAGCATGC CCGACGGCGA GGATCTCGTC 8521 GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA 8581 TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC 8641 CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT 8701 ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA 8761 GCGGGACTCT GGGGTTCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT 8821 TCGATTCCAC CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG 8881 GCTGGATGAT CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCTAGGGGGA 8941 GGCTAACTGA AACACGGAAG GAGACAATAC CGGAAGGAAC CCGCGCTATG ACGGCAATAA 9001 AAAGACAGAA TAAAACGCAC GGTGTTGGGT CGTTTGTTCA TAAACGCGGG GTTCGGTCCC 9061 AGGGCTGGCA CTCTGTCGAT ACCCCACCGA GACCCCATTG GGGCCAATAC GCCCGCGTTT 9121 CTTCCTTTTC CCCACCCCAC CCCCCAAGTT CGGGTGAAGG CCCAGGGCTC GCAGCCAACG 9181 TCGGGGCGGC AGGCCCTGCC ATAGCCTCAG GTTACTCATA TATACTTTAG ATTGATTTAA 9241 AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA 9301 AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG 9361 GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC 9421 CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA 9481 CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTTCTTCT AGTGTAGCCG TAGTTAGGCC 9541 ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG 9601 TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC 9661 CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC 9721 GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC 9781 CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA 9841 CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC 9901 TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG 9961 CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.