pLV3-U6-UBE2T-shRNA3-Puro plas

pLV3-U6-UBE2T-shRNA3-Puro plasmid

PVT96551

pLV3-U6-UBE2T-shRNA3-Puro plasmid Information

Plasmid name: pLV3-U6-UBE2T(human)-shRNA3-Puro
Alias: gagagagctgcacatgttagc
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Interference silence
Fragment type: shRNA
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction:

pLV3-U6-UBE2T-shRNA3-Puro plasmid Description

pLV3-U6-UBE2T-shRNA3-Puro This is a plasmid suitable for interference silencing in mammalian cells, and it can also be used for lentivirus packaging.

pLV3-U6-UBE2T-shRNA3-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtGAGGGCCT ATTTCCCATG ATTCCTTCAT ATTTGCATAT ACGATACAAG 1981 GCTGTTAGAG AGATAATTAG AATTAATTTG ACTGTAAACA CAAAGATATT AGTACAAAAT 2041 ACGTGACGTA GAAAGTAATA ATTTCTTGGG TAGTTTGCAG TTTTAAAATT ATGTTTTAAA 2101 ATGGACTATC ATATGCTTAC CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC 2161 TTGTGGAAAG GACTCATCgc tagcgaattc ggagagagct gcacatgtta gcTTCAAGAG 2221 Agctaacatg tgcagctctc tcTTTTTTgg atccgcggcc gcGaaggatc tgcgatcgct 2281 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag 2341 gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg 2401 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag 2461 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg aagcttcgag 2521 gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat ccacgccggt 2581 tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg 2641 taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg agcctaccta 2701 gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac gtctttgttt 2761 cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctacg ctagacGCCA 2821 CCatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg 2881 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg 2941 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg 3001 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg 3061 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg 3121 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg 3181 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg 3241 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc 3301 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg 3361 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct 3421 gaaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg 3481 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc 3541 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt 3601 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca 3661 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc 3721 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc 3781 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc 3841 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc 3901 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc 3961 tccgccttcg ccctcagacg agtcggatct ccctttggcc gcctccccgc ctggtacctt 4021 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg 4081 gactggaagg gctaattcac tcccaacgaa aataagatct gctttttgct tgtactgggt 4141 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc 4201 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg 4261 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta 4321 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga 4381 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa 4441 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca 4501 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca gttccgccca 4561 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc 4621 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctagact tttgcagaga 4681 cggcccaaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 4741 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 4801 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 4861 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 4921 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 4981 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 5041 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 5101 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 5161 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 5221 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 5281 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 5341 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 5401 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 5461 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 5521 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 5581 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 5641 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 5701 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 5761 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 5821 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 5881 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 5941 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 6001 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 6061 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 6121 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 6181 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 6241 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 6301 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 6361 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 6421 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 6481 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 6541 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 6601 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 6661 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 6721 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 6781 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 6841 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag 6901 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca 6961 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc 7021 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc 7081 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag 7141 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg 7201 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg 7261 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc 7321 caagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.