pLV3-U6-UBE2T-shRNA1-Puro plas

pLV3-U6-UBE2T-shRNA1-Puro plasmid

PVT96540

pLV3-U6-UBE2T-shRNA1-Puro plasmid Information

Plasmid name: pLV3-U6-UBE2T(human)-shRNA1-Puro
Alias: gcaactgtgttgacctctatt
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Interference silence
Fragment type: shRNA
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction:

pLV3-U6-UBE2T-shRNA1-Puro plasmid Description

pLV3-U6-UBE2T-shRNA1-Puro This is a plasmid suitable for interference silencing in mammalian cells, and it can also be used for lentivirus packaging.

pLV3-U6-UBE2T-shRNA1-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtGAGGGCCT ATTTCCCATG ATTCCTTCAT ATTTGCATAT ACGATACAAG 1981 GCTGTTAGAG AGATAATTAG AATTAATTTG ACTGTAAACA CAAAGATATT AGTACAAAAT 2041 ACGTGACGTA GAAAGTAATA ATTTCTTGGG TAGTTTGCAG TTTTAAAATT ATGTTTTAAA 2101 ATGGACTATC ATATGCTTAC CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC 2161 TTGTGGAAAG GACTCATCgc tagcgaattc ggcaactgtg ttgacctcta ttCTCGAGaa 2221 tagaggtcaa cacagttgcT TTTTTggatc cgcggccgcG aaggatctgc gatcgctccg 2281 gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg 2341 tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg 2401 tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg 2461 ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acagctgaag cttcgagggg 2521 ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg ccgccatcca cgccggttga 2581 gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa 2641 gtttaaagct caggtcgaga ccgggccttt gtccggcgct cccttggagc ctacctagac 2701 tcagccggct ctccacgctt tgcctgaccc tgcttgctca actctacgtc tttgtttcgt 2761 tttctgttct gcgccgttac agatccaagc tgtgaccggc gcctacgcta gacGCCACCa 2821 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac 2881 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc 2941 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca 3001 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga 3061 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt 3121 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc 3181 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca 3241 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg 3301 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg 3361 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa 3421 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc 3481 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta 3541 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt 3601 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg 3661 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta 3721 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt 3781 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg 3841 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca 3901 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtctcc 3961 gccttcgccc tcagacgagt cggatctccc tttggccgcc tccccgcctg gtacctttaa 4021 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac 4081 tggaagggct aattcactcc caacgaaaat aagatctgct ttttgcttgt actgggtctc 4141 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 4201 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 4261 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta 4321 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg 4381 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 4441 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 4501 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccagtt ccgcccattc 4561 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc 4621 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacgg 4681 cccaaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat 4741 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag 4801 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg 4861 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc 4921 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 4981 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 5041 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 5101 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 5161 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 5221 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 5281 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 5341 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 5401 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 5461 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 5521 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac 5581 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 5641 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 5701 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 5761 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 5821 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 5881 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 5941 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 6001 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 6061 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 6121 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 6181 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 6241 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 6301 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 6361 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 6421 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 6481 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 6541 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 6601 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 6661 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 6721 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 6781 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 6841 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg 6901 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat 6961 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg 7021 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga 7081 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag 7141 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc 7201 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt 7261 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa 7321 gctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.