pCMV-SmBit-AP2A1 plasmid

pCMV-SmBit-AP2A1 plasmid

PVT96523

pCMV-SmBit-AP2A1 plasmid Information

Plasmid name: pCMV-SmBit-AP2A1(human)
Alias: NM_014203.3;ADTAA; AP2-ALPHA; CLAPA1
Gene lenght: 2934bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: SP6
Reverse primer:
Copy number: High
Induction:

pCMV-SmBit-AP2A1 plasmid Description

pCMV-SmBit-AP2A1 is a plasmid suitable for protein expression in mammalian cells.

pCMV-SmBit-AP2A1 plasmid Sequence

1 TTCGAGCTCG CCCGACATTG ATTATTGACT AGTTATTAAT AGTAATCAAT TACGGGGTCA 61 TTAGTTCATA GCCCATATAT GGAGTTCCGC GTTACATAAC TTACGGTAAA TGGCCCGCCT 121 GGCTGACCGC CCAACGACCC CCGCCCATTG ACGTCAATAA TGACGTATGT TCCCATAGTA 181 ACGCCAATAG GGACTTTCCA TTGACGTCAA TGGGTGGAGT ATTTACGGTA AACTGCCCAC 241 TTGGCAGTAC ATCAAGTGTA TCATATGCCA AGTACGCCCC CTATTGACGT CAATGACGGT 301 AAATGGCCCG CCTGGCATTA TGCCCAGTAC ATGACCTTAT GGGACTTTCC TACTTGGCAG 361 TACATCTACG TATTAGTCAT CGCTATTACC ATGGTGATGC GGTTTTGGCA GTACATCAAT 421 GGGCGTGGAT AGCGGTTTGA CTCACGGGGA TTTCCAAGTC TCCACCCCAT TGACGTCAAT 481 GGGAGTTTGT TTTGGCACCA AAATCAACGG GACTTTCCAA AATGTCGTAA CAACTCCGCC 541 CCATTGACGC AAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG CAGAGCTCGT 601 TTAGTGAACC GTCAGATCGC CTGGAGACGC CATCCACGCT GTTTTGACCT CCATAGAAGA 661 CACCGGGACC GATCCAGCCT CCGCGGCCGG GAACGGTGCA TTGGAACGCG GATTCCCCGT 721 GCCAAGAGTG ACGTAAGTAC CGCCTATAGA GTCTATAGGC CCACCCCCTT GGCTTCGTTA 781 GAACGCGGCT ACAATTAATA CATAACCTTA TGTATCATAC ACATACGATT TAGGTGACAC 841 TATAGAATAA CATCCACTTT GCCTTTCTCT CCACAGGTGT CCACTCCCAG GTCCAACTGC 901 ACCTCGGTTC TATCGATTGA ATTCATGGTG ACCGGCTACC GGCTGTTCGA GGAGATTCTG 961 GGTGGAGGCG GTTCAGGAGG TGGCGGATCC atgccggccg tgtccaaggg cgatgggatg 1021 cgggggctcg cggtgttcat ctccgacatc cggaactgta agagcaaaga ggcggaaatt 1081 aagagaatca acaaggaact ggccaacatc cgctccaagt tcaaaggaga caaagccttg 1141 gatggctaca gtaagaaaaa atatgtgtgt aaactgcttt tcatcttcct gcttggccat 1201 gacattgact ttgggcacat ggaggctgtg aatctgttga gttccaataa atacacagag 1261 aagcaaatag gttacctgtt catttctgtg ctggtgaact cgaactcgga gctgatccgc 1321 ctcatcaaca acgccatcaa gaatgacctg gccagccgca accccacctt catgtgcctg 1381 gccctgcact gcatcgccaa cgtgggcagc cgggagatgg gcgaggcctt tgccgctgac 1441 atcccccgca tcctggtggc cggggacagc atggacagtg tcaagcagag tgcggccctg 1501 tgcctccttc gactgtacaa ggcctcgcct gacctggtgc ccatgggcga gtggacggcg 1561 cgtgtggtac acctgctcaa tgaccagcac atgggtgtgg tcacggccgc cgtcagcctc 1621 atcacctgtc tctgcaagaa gaacccagat gacttcaaga cgtgcgtctc tctggctgtg 1681 tcgcgcctga gccggatcgt ctcctctgcc tccaccgacc tccaggacta cacctactac 1741 ttcgtcccag caccctggct ctcggtgaag ctcctgcggc tgctgcagtg ctacccgcct 1801 ccagaggatg cggctgtgaa ggggcggctg gtggaatgtc tggagactgt gctcaacaag 1861 gcccaggagc cccccaaatc caagaaggtg cagcattcca acgccaagaa cgccatcctc 1921 ttcgagacca tcagcctcat catccactat gacagtgagc ccaacctcct ggttcgggcc 1981 tgcaaccagc tgggccagtt cctgcagcac cgggagacca acctgcgcta cctggccctg 2041 gagagcatgt gcacgctggc cagctccgag ttctcccatg aagccgtcaa gacgcacatt 2101 gacaccgtca tcaatgccct caagacggag cgggacgtca gcgtgcggca gcgggcggct 2161 gacctcctct acgccatgtg tgaccggagc aatgccaagc agatcgtgtc ggagatgctg 2221 cggtacctgg agacggcaga ctacgccatc cgcgaggaga tcgtcctgaa ggtggccatc 2281 ctggccgaga agtacgccgt ggactacagc tggtacgtgg acaccatcct caacctcatc 2341 cgcattgcgg gcgactacgt gagtgaggag gtgtggtacc gtgtgctaca gatcgtcacc 2401 aaccgtgatg acgtccaggg ctatgccgcc aagaccgtct ttgaggcgct ccaggcccct 2461 gcctgtcacg agaacatggt gaaggttggc ggctacatcc ttggggagtt tgggaacctg 2521 attgctgggg acccccgctc cagcccccca gtgcagttct ccctgctcca ctccaagttc 2581 catctgtgca gcgtggccac gcgggcgctg ctgctgtcca cctacatcaa gttcatcaac 2641 ctcttccccg agaccaaggc caccatccag ggcgtcctgc gggccggctc ccagctgcgc 2701 aatgctgacg tggagctgca gcagcgagcc gtggagtacc tcaccctcag ctcagtggcc 2761 agcaccgacg tcctggccac ggtgctggag gagatgccgc ccttccccga gcgcgagtcg 2821 tccatcctgg ccaagctgaa acgcaagaag gggccagggg ccggcagcgc cctggacgat 2881 ggccggaggg accccagcag caacgacatc aacgggggca tggagcccac ccccagcact 2941 gtgtcgacgc cctcgccctc cgccgacctc ctggggctgc gggcagcccc tcccccggca 3001 gcacccccgg cttctgcagg agcagggaac cttctggtgg acgtcttcga tggcccggcc 3061 gcccagccca gcctggggcc cacccccgag gaggccttcc tcagcgagct ggagccgcct 3121 gcccccgaga gccccatggc tttgctggct gacccagctc cagctgctga cccaggtcct 3181 gaggacatcg gccctcccat tccggaagcc gatgagttgc tgaataagtt tgtgtgtaag 3241 aacaacgggg tcctgttcga gaaccagctg ctgcagatcg gagtcaagtc agagttccga 3301 cagaacctgg gccgcatgta tctcttctat ggcaacaaga cctcggtgca gttccagaat 3361 ttctcaccca ctgtggttca cccgggagac ctccagactc agctggctgt gcagaccaag 3421 cgcgtggcgg cgcaggtgga cggcggcgcg caggtgcagc aggtgctcaa tatcgagtgc 3481 ctgcgggact tcctgacgcc cccgctgctg tccgtgcgct tccggtacgg tggcgccccc 3541 caggccctca ccctgaagct cccagtgacc atcaacaagt tcttccagcc caccgagatg 3601 gcggcccagg atttcttcca gcgctggaag cagctgagcc tccctcaaca ggaggcgcag 3661 aaaatcttca aagccaacca ccccatggac gcagaagtta ctaaggccaa gcttctgggg 3721 tttggctctg ctctcctgga caatgtggac cccaaccctg agaacttcgt gggggcgggg 3781 atcatccaga ctaaagccct gcaggtgggc tgtctgcttc ggctggagcc caatgcccag 3841 gcccagatgt accggctgac cctgcgcacc agcaaggagc ccgtctcccg tcacctgtgt 3901 gagctgctgg cacagcagtt ctgaAAGCTT GGCCGCCATG GCCCAACTTG TTTATTGCAG 3961 CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT 4021 CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGGATCG 4081 GGAATTAATT CGGCGCAGCA CCATGGCCTG AAATAACCTC TGAAAGAGGA ACTTGGTTAG 4141 GTACCTTCTG AGGCGGAAAG AACCAGCTGT GGAATGTGTG TCAGTTAGGG TGTGGAAAGT 4201 CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA 4261 GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT 4321 AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT 4381 CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG 4441 CCTCGGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT 4501 GCAAAAAGCT GTTAACAGCT TGGCACTGGC CGTCGTTTTA CAACGTCGTG ACTGGGAAAA 4561 CCCTGGCGTT ACCCAACTTA ATCGCCTTGC AGCACATCCC CCTTTCGCCA GCTGGCGTAA 4621 TAGCGAAGAG GCCCGCACCG ATCGCCCTTC CCAACAGTTG CGCAGCCTGA ATGGCGAATG 4681 GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATACGTCA 4741 AAGCAACCAT AGTACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 4801 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 4861 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 4921 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTT GGGTGATGGT 4981 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 5041 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGGCTAT 5101 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 5161 TAACAAAAAT TTAACGCGAA TTTTAACAAA ATATTAACGT TTACAATTTT ATGGTGCACT 5221 CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGC CCCGACACCC GCCAACACCC 5281 GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC 5341 GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGACGA 5401 AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG 5461 ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA 5521 ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT 5581 TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG 5641 GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA 5701 GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT 5761 GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT 5821 GGCGCGGTAT TATCCCGTAT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT 5881 TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG 5941 ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA 6001 CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT 6061 CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG 6121 CGTGACACCA CGATGCCTGT AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA 6181 CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA 6241 GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC 6301 GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT 6361 ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC 6421 GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT 6481 ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT 6541 TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC 6601 CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC 6661 TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA 6721 ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTTCTTCTA 6781 GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT 6841 CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG 6901 GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC 6961 ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA 7021 TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG 7081 GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT 7141 CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG 7201 CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG 7261 CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC 7321 GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG 7381 AGCGAGGAAG CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT 7441 CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGCAACGCA 7501 ATTAATGTGA GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT 7561 CGTATGTTGT GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG CTATGACATG 7621 ATTACGAATT AA

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.