pCMV-mRuby3-UTP15-Neo plasmid

pCMV-mRuby3-UTP15-Neo plasmid

PVT94105

pCMV-mRuby3-UTP15-Neo plasmid Information

Plasmid name: pCMV-mRuby3-UTP15(human)-Neo
Alias: NM_001436.4;FIB; FLRN; Nop1; RNU3IP1;NM_032175.4;NET21;
Gene lenght: 963，1557bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: Sv40-polyA-R
Copy number: High
Induction:

pCMV-mRuby3-UTP15-Neo plasmid Description

pCMV-mRuby3-UTP15-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-mRuby3-UTP15-Neo plasmid Sequence

1 TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG 61 CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT 121 GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA 181 ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC 241 AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA 301 CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC 361 CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG 421 ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG 481 GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT 541 ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA 601 CCGGTCGCCA CCatggtgtc taagggcgaa gagctgatca aggaaaatat gcgtatgaag 661 gtggtcatgg aaggttcggt caacggccac caattcaaat gcacaggtga aggagaaggc 721 agaccgtacg agggagtgca aaccatgagg atcaaagtca tcgagggagg acccctgcca 781 tttgcctttg acattcttgc cacgtcgttc atgtatggca gccgtacctt tatcaagtac 841 ccggccgaca tccctgattt ctttaaacag tcctttcctg agggttttac ttgggaaaga 901 gttacgagat acgaagatgg tggagtcgtc accgtcacgc aggacaccag ccttgaggat 961 ggcgagctcg tctacaacgt caaggtcaga ggggtaaact ttccctccaa tggtcccgtg 1021 atgcagaaga agaccaaggg ttgggagcct aatacagaga tgatgtatcc agcagatggt 1081 ggtctgagag gatacactga catcgcactg aaagttgatg gtggtggcca tctgcactgc 1141 aacttcgtga caacttacag gtcaaaaaag accgtcggga acatcaagat gcccggtgtc 1201 catgccgttg atcaccgcct ggaaaggatc gaggagagtg acaatgaaac ctacgtagtg 1261 caaagagaag tggcagttgc caaatacagc aaccttggtg gtggcatgga cgagctgtac 1321 aagTCCGGAC TCAGATCTCG AGCTCAAGCT TCCatggctg gttataagcc tgtagctatt 1381 cagacatatc ctatacttgg tgaaaaaatc acccaagata cactgtactg gaacaactat 1441 aagacccctg ttcagattaa ggaatttggt gcagtttcaa aagtagactt ttctcctcag 1501 cctccatata attatgctgt cacagcttcc tcaagaattc acatttatgg ccgatactcc 1561 caagaaccta taaaaacctt ttctcgattt aaagacacag catactgtgc tacttttcga 1621 caagatggta gattgcttgt ggctggcagt gaagatggtg gagttcaact ttttgatata 1681 agtgggaggg ctcccctcag gcagtttgaa ggccatacaa aagcagttca tacagtagat 1741 tttacagctg acaaatatca cgtggtctct ggggctgatg attatacagt taaattatgg 1801 gatattccaa actccaaaga aattttgaca tttaaagaac actctgatta tgtgaggtgt 1861 ggatgtgcta gcaaacttaa tccggatctc tttataacag gatcatatga tcatactgtg 1921 aagatgtttg atgcacgaac gagtgagagt gttctctccg ttgagcatgg gcagccagtg 1981 gagagtgtcc tacttttccc ctctggaggt cttctggtgt cagcaggagg tcgttatgtt 2041 aaagtctggg acatgttaaa aggaggacaa ttgctagtat ctttgaaaaa tcatcacaaa 2101 accgtgacat gtttatgtct aagcagctct ggacagaggt tactctctgg ctcactggat 2161 aggaaggtga aagtatacag cacaacttcc tacaaagtag tccacagttt tgattatgca 2221 gcttcaattt tgagtcttgc ccttgcacat gaagatgaga caatagttgt aggaatgacc 2281 aatggaatac tgagtgttaa acatcggaaa tctgaagcaa agaaggaatc acttcccaga 2341 agaagaaggc ctgcatatcg aacctttatt aaaggaaaaa attacatgaa gcaacgggat 2401 gacattttga ttaacaggcc agcaaagaag cacctagaat tgtatgacag ggatctgaaa 2461 cattttcgga tctctaaggc actcgataga gttcttgatc ccacttgtac aataaagaca 2521 cccgagatta cggtgtccat cataaaggag ttaaatcgaa gaggagtcct tgcaaatgcg 2581 cttgcaggtc gggatgagaa ggaaatcagt catgttctta attttttgat aaggaatctt 2641 tctcagccaa gatttgcccc tgttttaatc aatgctgctg aaataattat tgatatatat 2701 ctgcctgtaa ttggtcagtc ccctgtagtt gataaaaagt ttttactact tcaaggactt 2761 gtagaaaaag agattgatta ccaaagagaa ttgttagaaa ccttggggat gatggatatg 2821 ctttttgcca ccatgagaag gaaggaaggc acttctgtgt tggaacacac atctgatgga 2881 tttccagaga ataagaagat agaatcatag GGTACCGCGG GCCCGGGATC CACCGGATCT 2941 AGATAACTGA TCATAATCAG CCATACCACA TTTGTAGAGG TTTTACTTGC TTTAAAAAAC 3001 CTCCCACACC TCCCCCTGAA CCTGAAACAT AAAATGAATG CAATTGTTGT TGTTAACTTG 3061 TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA 3121 GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTAACGC 3181 GTAAATTGTA AGCGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC 3241 ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA 3301 GATAGGGTTG AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC 3361 CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG AACCATCACC 3421 CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA AATCGGAACC CTAAAGGGAG 3481 CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG GCGAGAAAGG AAGGGAAGAA 3541 AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC 3601 CACACCCGCC GCGCTTAATG CGCCGCTACA GGGCGCGTCA GGTGGCACTT TTCGGGGAAA 3661 TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT 3721 GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTC CTGAGGCGGA 3781 AAGAACCAGC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA 3841 GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA 3901 GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC 3961 CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC 4021 CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA 4081 TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAGA TCGATCAAGA 4141 GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC 4201 CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA 4261 TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT 4321 GTCCGGTGCC CTGAATGAAC TGCAAGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC 4381 GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT 4441 ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT 4501 ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT 4561 CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT 4621 CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG 4681 GCTCAAGGCG AGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT 4741 GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG 4801 TGTGGCGGAC CGCTATCAGG ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG 4861 CGGCGAATGG GCTGACCGCT TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG 4921 CATCGCCTTC TATCGCCTTC TTGACGAGTT CTTCTGAGCG GGACTCTGGG GTTCGAAATG 4981 ACCGACCAAG CGACGCCCAA CCTGCCATCA CGAGATTTCG ATTCCACCGC CGCCTTCTAT 5041 GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG 5101 GATCTCATGC TGGAGTTCTT CGCCCACCCT AGGGGGAGGC TAACTGAAAC ACGGAAGGAG 5161 ACAATACCGG AAGGAACCCG CGCTATGACG GCAATAAAAA GACAGAATAA AACGCACGGT 5221 GTTGGGTCGT TTGTTCATAA ACGCGGGGTT CGGTCCCAGG GCTGGCACTC TGTCGATACC 5281 CCACCGAGAC CCCATTGGGG CCAATACGCC CGCGTTTCTT CCTTTTCCCC ACCCCACCCC 5341 CCAAGTTCGG GTGAAGGCCC AGGGCTCGCA GCCAACGTCG GGGCGGCAGG CCCTGCCATA 5401 GCCTCAGGTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG 5461 ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG 5521 TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT 5581 CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG 5641 CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA 5701 CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA 5761 CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG 5821 TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC 5881 TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA 5941 TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG 6001 TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC 6061 GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG 6121 TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG 6181 TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT 6241 GTGGATAACC GTATTACCGC CATGCAT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.