pCMV-AP2A1-SmBit plasmid

pCMV-AP2A1-SmBit plasmid

PVT97079

pCMV-AP2A1-SmBit plasmid Information

Plasmid name: pCMV-AP2A1(human)-SmBit
Alias: NM_014203.3;ADTAA; AP2-ALPHA; CLAPA1
Gene lenght: 2934bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: SP6
Reverse primer:
Copy number: High
Induction:

pCMV-AP2A1-SmBit plasmid Description

pCMV-AP2A1-SmBit is a plasmid suitable for protein expression in mammalian cells.

pCMV-AP2A1-SmBit plasmid Sequence

1 TTCGAGCTCG CCCGACATTG ATTATTGACT AGTTATTAAT AGTAATCAAT TACGGGGTCA 61 TTAGTTCATA GCCCATATAT GGAGTTCCGC GTTACATAAC TTACGGTAAA TGGCCCGCCT 121 GGCTGACCGC CCAACGACCC CCGCCCATTG ACGTCAATAA TGACGTATGT TCCCATAGTA 181 ACGCCAATAG GGACTTTCCA TTGACGTCAA TGGGTGGAGT ATTTACGGTA AACTGCCCAC 241 TTGGCAGTAC ATCAAGTGTA TCATATGCCA AGTACGCCCC CTATTGACGT CAATGACGGT 301 AAATGGCCCG CCTGGCATTA TGCCCAGTAC ATGACCTTAT GGGACTTTCC TACTTGGCAG 361 TACATCTACG TATTAGTCAT CGCTATTACC ATGGTGATGC GGTTTTGGCA GTACATCAAT 421 GGGCGTGGAT AGCGGTTTGA CTCACGGGGA TTTCCAAGTC TCCACCCCAT TGACGTCAAT 481 GGGAGTTTGT TTTGGCACCA AAATCAACGG GACTTTCCAA AATGTCGTAA CAACTCCGCC 541 CCATTGACGC AAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG CAGAGCTCGT 601 TTAGTGAACC GTCAGATCGC CTGGAGACGC CATCCACGCT GTTTTGACCT CCATAGAAGA 661 CACCGGGACC GATCCAGCCT CCGCGGCCGG GAACGGTGCA TTGGAACGCG GATTCCCCGT 721 GCCAAGAGTG ACGTAAGTAC CGCCTATAGA GTCTATAGGC CCACCCCCTT GGCTTCGTTA 781 GAACGCGGCT ACAATTAATA CATAACCTTA TGTATCATAC ACATACGATT TAGGTGACAC 841 TATAGAATAA CATCCACTTT GCCTTTCTCT CCACAGGTGT CCACTCCCAG GTCCAACTGC 901 ACCTCGGTTC TATCGATTGA ATTCatgccg gccgtgtcca agggcgatgg gatgcggggg 961 ctcgcggtgt tcatctccga catccggaac tgtaagagca aagaggcgga aattaagaga 1021 atcaacaagg aactggccaa catccgctcc aagttcaaag gagacaaagc cttggatggc 1081 tacagtaaga aaaaatatgt gtgtaaactg cttttcatct tcctgcttgg ccatgacatt 1141 gactttgggc acatggaggc tgtgaatctg ttgagttcca ataaatacac agagaagcaa 1201 ataggttacc tgttcatttc tgtgctggtg aactcgaact cggagctgat ccgcctcatc 1261 aacaacgcca tcaagaatga cctggccagc cgcaacccca ccttcatgtg cctggccctg 1321 cactgcatcg ccaacgtggg cagccgggag atgggcgagg cctttgccgc tgacatcccc 1381 cgcatcctgg tggccgggga cagcatggac agtgtcaagc agagtgcggc cctgtgcctc 1441 cttcgactgt acaaggcctc gcctgacctg gtgcccatgg gcgagtggac ggcgcgtgtg 1501 gtacacctgc tcaatgacca gcacatgggt gtggtcacgg ccgccgtcag cctcatcacc 1561 tgtctctgca agaagaaccc agatgacttc aagacgtgcg tctctctggc tgtgtcgcgc 1621 ctgagccgga tcgtctcctc tgcctccacc gacctccagg actacaccta ctacttcgtc 1681 ccagcaccct ggctctcggt gaagctcctg cggctgctgc agtgctaccc gcctccagag 1741 gatgcggctg tgaaggggcg gctggtggaa tgtctggaga ctgtgctcaa caaggcccag 1801 gagcccccca aatccaagaa ggtgcagcat tccaacgcca agaacgccat cctcttcgag 1861 accatcagcc tcatcatcca ctatgacagt gagcccaacc tcctggttcg ggcctgcaac 1921 cagctgggcc agttcctgca gcaccgggag accaacctgc gctacctggc cctggagagc 1981 atgtgcacgc tggccagctc cgagttctcc catgaagccg tcaagacgca cattgacacc 2041 gtcatcaatg ccctcaagac ggagcgggac gtcagcgtgc ggcagcgggc ggctgacctc 2101 ctctacgcca tgtgtgaccg gagcaatgcc aagcagatcg tgtcggagat gctgcggtac 2161 ctggagacgg cagactacgc catccgcgag gagatcgtcc tgaaggtggc catcctggcc 2221 gagaagtacg ccgtggacta cagctggtac gtggacacca tcctcaacct catccgcatt 2281 gcgggcgact acgtgagtga ggaggtgtgg taccgtgtgc tacagatcgt caccaaccgt 2341 gatgacgtcc agggctatgc cgccaagacc gtctttgagg cgctccaggc ccctgcctgt 2401 cacgagaaca tggtgaaggt tggcggctac atccttgggg agtttgggaa cctgattgct 2461 ggggaccccc gctccagccc cccagtgcag ttctccctgc tccactccaa gttccatctg 2521 tgcagcgtgg ccacgcgggc gctgctgctg tccacctaca tcaagttcat caacctcttc 2581 cccgagacca aggccaccat ccagggcgtc ctgcgggccg gctcccagct gcgcaatgct 2641 gacgtggagc tgcagcagcg agccgtggag tacctcaccc tcagctcagt ggccagcacc 2701 gacgtcctgg ccacggtgct ggaggagatg ccgcccttcc ccgagcgcga gtcgtccatc 2761 ctggccaagc tgaaacgcaa gaaggggcca ggggccggca gcgccctgga cgatggccgg 2821 agggacccca gcagcaacga catcaacggg ggcatggagc ccacccccag cactgtgtcg 2881 acgccctcgc cctccgccga cctcctgggg ctgcgggcag cccctccccc ggcagcaccc 2941 ccggcttctg caggagcagg gaaccttctg gtggacgtct tcgatggccc ggccgcccag 3001 cccagcctgg ggcccacccc cgaggaggcc ttcctcagcg agctggagcc gcctgccccc 3061 gagagcccca tggctttgct ggctgaccca gctccagctg ctgacccagg tcctgaggac 3121 atcggccctc ccattccgga agccgatgag ttgctgaata agtttgtgtg taagaacaac 3181 ggggtcctgt tcgagaacca gctgctgcag atcggagtca agtcagagtt ccgacagaac 3241 ctgggccgca tgtatctctt ctatggcaac aagacctcgg tgcagttcca gaatttctca 3301 cccactgtgg ttcacccggg agacctccag actcagctgg ctgtgcagac caagcgcgtg 3361 gcggcgcagg tggacggcgg cgcgcaggtg cagcaggtgc tcaatatcga gtgcctgcgg 3421 gacttcctga cgcccccgct gctgtccgtg cgcttccggt acggtggcgc cccccaggcc 3481 ctcaccctga agctcccagt gaccatcaac aagttcttcc agcccaccga gatggcggcc 3541 caggatttct tccagcgctg gaagcagctg agcctccctc aacaggaggc gcagaaaatc 3601 ttcaaagcca accaccccat ggacgcagaa gttactaagg ccaagcttct ggggtttggc 3661 tctgctctcc tggacaatgt ggaccccaac cctgagaact tcgtgggggc ggggatcatc 3721 cagactaaag ccctgcaggt gggctgtctg cttcggctgg agcccaatgc ccaggcccag 3781 atgtaccggc tgaccctgcg caccagcaag gagcccgtct cccgtcacct gtgtgagctg 3841 ctggcacagc agttcGTCGA CGGTGGAGGC GGTTCAGGAG GTGGCGGATC TATGGTGACC 3901 GGCTACCGGC TGTTCGAGGA GATTCTGTAA AAGCTTGGCC GCCATGGCCC AACTTGTTTA 3961 TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT 4021 TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT 4081 GGATCGGGAA TTAATTCGGC GCAGCACCAT GGCCTGAAAT AACCTCTGAA AGAGGAACTT 4141 GGTTAGGTAC CTTCTGAGGC GGAAAGAACC AGCTGTGGAA TGTGTGTCAG TTAGGGTGTG 4201 GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG 4261 CAACCAGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC 4321 TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC 4381 CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG 4441 AGGCCGCCTC GGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG 4501 GCTTTTGCAA AAAGCTGTTA ACAGCTTGGC ACTGGCCGTC GTTTTACAAC GTCGTGACTG 4561 GGAAAACCCT GGCGTTACCC AACTTAATCG CCTTGCAGCA CATCCCCCTT TCGCCAGCTG 4621 GCGTAATAGC GAAGAGGCCC GCACCGATCG CCCTTCCCAA CAGTTGCGCA GCCTGAATGG 4681 CGAATGGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT 4741 ACGTCAAAGC AACCATAGTA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 4801 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 4861 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 4921 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTTGGGT 4981 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 5041 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 5101 GGCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 5161 CTGATTTAAC AAAAATTTAA CGCGAATTTT AACAAAATAT TAACGTTTAC AATTTTATGG 5221 TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGCCCCG ACACCCGCCA 5281 ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT 5341 GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG 5401 AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT 5461 TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT 5521 TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA 5581 TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT 5641 TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT 5701 GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG 5761 ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG 5821 CTATGTGGCG CGGTATTATC CCGTATTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA 5881 CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT 5941 GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC 6001 AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG 6061 GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC 6121 GACGAGCGTG ACACCACGAT GCCTGTAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT 6181 GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA 6241 GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT 6301 GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC 6361 TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA 6421 CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC 6481 TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG 6541 ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG 6601 TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC 6661 TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG 6721 CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTT 6781 CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC 6841 CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC 6901 GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT 6961 TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT 7021 GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC 7081 GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT 7141 TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA 7201 GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT 7261 TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT 7321 ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG 7381 TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA ATACGCAAAC CGCCTCTCCC CGCGCGTTGG 7441 CCGATTCATT AATGCAGCTG GCACGACAGG TTTCCCGACT GGAAAGCGGG CAGTGAGCGC 7501 AACGCAATTA ATGTGAGTTA GCTCACTCAT TAGGCACCCC AGGCTTTACA CTTTATGCTT 7561 CCGGCTCGTA TGTTGTGTGG AATTGTGAGC GGATAACAAT TTCACACAGG AAACAGCTAT 7621 GACATGATTA CGAATTAA

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.