pLV3-CMV-DSG2-3×FLAG-CopGFP-Pu

pLV3-CMV-DSG2-3×FLAG-CopGFP-Puro plasmid

PVT96511

pLV3-CMV-DSG2-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-DSG2(human)-3×FLAG-CopGFP-Puro
Alias: NM_001943.5;CDHF5; HDGC
Gene lenght: 3354bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-DSG2-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-DSG2-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-DSG2-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg cgcggagccc gggacgcgcg 2341 tacgccctgc tgcttctcct gatctgcttt aacgttggaa gtggacttca cttacaggtc 2401 ttaagcacaa gaaatgaaaa taagctgctt cctaaacatc ctcatttagt gcggcaaaag 2461 cgcgcctgga tcaccgcccc cgtggctctt cgggagggag aggatctgtc caagaagaat 2521 ccaattgcca agatacattc tgatcttgca gaagaaagag gactcaaaat tacttacaaa 2581 tacactggaa aagggattac agagccacct tttggtatat ttgtctttaa caaagatact 2641 ggagaactga atgttaccag cattcttgat cgagaagaaa caccattttt tctgctaaca 2701 ggttacgctt tggatgcaag aggaaacaat gtagagaaac ccttagagct acgcattaag 2761 gttcttgata tcaatgacaa cgaaccagtg ttcacacagg atgtctttgt tgggtctgtt 2821 gaagagttga gtgcagcaca tactcttgtg atgaaaatca atgcaacaga tgcagatgag 2881 cccaataccc tgaattcgaa aatttcctat agaatcgtat ctctggagcc tgcttatcct 2941 ccagtgttct acctaaataa agatacagga gagatttata caaccagtgt taccttggac 3001 agagaggaac acagcagcta cactttgaca gtagaagcaa gagatggcaa tggagaagtt 3061 acagacaaac ctgtaaaaca agctcaagtt cagattcgta ttttggatgt caatgacaat 3121 atacctgtag tagaaaataa agtgcttgaa gggatggttg aagaaaatca agtcaacgta 3181 gaagttacgc gcataaaagt gttcgatgca gatgaaatag gttctgataa ttggctggca 3241 aattttacat ttgcatcagg aaatgaagga ggttatttcc acatagaaac agatgctcaa 3301 actaacgaag gaattgtgac ccttattaag gaagtagatt atgaagaaat gaagaatctt 3361 gacttcagtg ttattgtcgc taataaagca gcttttcaca agtcgattag gagtaaatac 3421 aagcctacac ccattcccat caaggtcaaa gtgaaaaatg tgaaagaagg cattcatttt 3481 aaaagcagcg tcatctcaat ttatgttagc gagagcatgg atagatcaag caaaggccaa 3541 ataattggaa attttcaagc ttttgatgag gacactggac taccagccca tgcaagatat 3601 gtaaaattag aagatagaga taattggatc tctgtggatt ctgtcacatc tgaaattaaa 3661 cttgcaaaac ttcctgattt tgaatctaga tatgttcaaa atggcacata cactgtaaag 3721 attgtggcca tatcagaaga ttatcctaga aaaaccatca ctggcacagt ccttatcaat 3781 gttgaagaca tcaacgacaa ctgtcccaca ctgatagagc ctgtgcagac aatctgtcac 3841 gatgcagagt atgtgaatgt tactgcagag gacctggatg gacacccaaa cagtggccct 3901 ttcagtttct ccgtcattga caaaccacct ggcatggcag aaaaatggaa aatagcacgc 3961 caagaaagta ccagtgtgct gctgcaacaa agtgagaaaa agcttgggag aagtgaaatt 4021 cagttcctga tttcagacaa tcagggtttt agttgtcctg aaaagcaggt ccttacactc 4081 acagtttgtg agtgtctgca tggcagcggc tgcagggaag cacagcatga ctcctatgtg 4141 ggcctgggac ccgcagcaat tgcgctcatg attttggcct ttctgctcct gctattggta 4201 ccacttttac tgctgatgtg ccattgcgga aagggcgcca aaggctttac ccccatacct 4261 ggcaccatag agatgctgca tccttggaat aatgaaggag caccacctga agacaaggtg 4321 gtgccatcat ttctgccagt ggatcaaggg ggcagtctag taggaagaaa tggagtagga 4381 ggtatggcca aggaagccac gatgaaagga agtagctctg cttccattgt caaagggcaa 4441 catgagatgt ccgagatgga tggaaggtgg gaagaacaca gaagcctgct ttctggtaga 4501 gctacccagt ttacaggggc cacaggcgct atcatgacca ctgaaaccac gaagaccgca 4561 agggccacag gggcttccag agacatggcc ggagctcagg cagctgctgt tgcactgaac 4621 gaagaattct taagaaatta tttcactgat aaagcggcct cttacactga ggaagatgaa 4681 aatcacacag ccaaagattg ccttctggtt tattctcagg aagaaactga atcgctgaat 4741 gcttctattg gttgttgcag ttttattgaa ggagagctag atgaccgctt cttagatgat 4801 ttgggactta aattcaagac actagctgaa gtttgcctgg gtcaaaaaat agatataaat 4861 aaggaaattg agcagagaca aaaacctgcc acagaaacaa gtatgaacac agcttcacat 4921 tcactctgtg agcaaactat ggttaattca gagaatacct actcctctgg cagtagcttc 4981 ccagttccaa aatctttgca agaagccaat gcagagaaag taactcagga aatagtcact 5041 gaaagatctg tgtcttctag gcaggcgcaa aaggtagcta cacctcttcc tgacccaatg 5101 gcttctagaa atgtgatagc aacagaaact tcctatgtca cagggtccac tatgccacca 5161 accactgtga tcctgggtcc tagccagcca cagagcctta ttgtgacaga gagggtgtat 5221 gctccagctt ctaccttggt agatcagcct tatgctaatg aaggtacagt tgtggtcact 5281 gaaagagtaa tacagcctca tgggggtgga tcgaatcctc tggaaggcac tcagcatctt 5341 caagatgtac cttacgtcat ggtgagggaa agagagagct tccttgcccc cagctcaggt 5401 gtgcagccta ctctggccat gcctaatata gcagtaggac agaatgtgac agtgacagaa 5461 agagttctag cacctgcttc cactctgcaa tccagttacc agattcccac tgaaaattct 5521 atgacggcta ggaacaccac ggtgtctgga gctggagtcc ctggccctct gccagatttt 5581 ggtttagagg aatctggtca ttctaattct accataacca catcttccac cagagttacc 5641 aagcatagca ctgtacagca ttcttactcc ggatccGACT ACAAAGACCA TGACGGTGAT 5701 TATAAAGATC ATGACATCGA CTACAAGGAT GACGATGACA AGtagcggcc gcGaaggatc 5761 tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag 5821 ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg 5881 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata 5941 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg 6001 aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat 6061 ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc 6121 gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg 6181 agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac 6241 gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctacg 6301 ctagacgcca ccatggagag cgacgagagc ggcctgcccg ccatggagat cgagtgccgc 6361 atcaccggca ccctgaacgg cgtggagttc gagctggtgg gcggcggaga gggcaccccc 6421 aagcagggcc gcatgaccaa caagatgaag agcaccaaag gcgccctgac cttcagcccc 6481 tacctgctga gccacgtgat gggctacggc ttctaccact tcggcaccta ccccagcggc 6541 tacgagaacc ccttcctgca cgccatcaac aacggcggct acaccaacac ccgcatcgag 6601 aagtacgagg acggcggcgt gctgcacgtg agcttcagct accgctacga ggccggccgc 6661 gtgatcggcg acttcaaggt ggtgggcacc ggcttccccg aggacagcgt gatcttcacc 6721 gacaagatca tccgcagcaa cgccaccgtg gagcacctgc accccatggg cgataacgtg 6781 ctggtgggca gcttcgcccg caccttcagc ctgcgcgacg gcggctacta cagcttcgtg 6841 gtggacagcc acatgcactt caagagcgcc atccacccca gcatcctgca gaacgggggc 6901 cccatgttcg ccttccgccg cgtggaggag ctgcacagca acaccgagct gggcatcgtg 6961 gagtaccagc acgccttcaa gacccccatc gccttcgcca gatcccgcgc tcagtcgtcc 7021 aattctgccg tggacggcac cgccggaccc ggctccaccg gatctcgcga gggcagagga 7081 agtcttctaa catgcggtga cgtggaggag aatcccggcc ctatgaccga gtacaagccc 7141 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg 7201 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc 7261 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc 7321 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg 7381 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag 7441 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc 7501 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc 7561 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc 7621 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa 7681 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaaatcaacc tctggattac 7741 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga 7801 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc 7861 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa 7921 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc 7981 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc 8041 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc 8101 gtggtgttgt cggggaaATC ATCGTCCTTT CCTtggctgc tcgcctgtgt tgccacctgg 8161 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct 8221 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc tTcgccttcg ccctcagacg 8281 agtcggatct ccctttggGc cgcctccccg cctggtacct ttaagaccaa tgacttacaa 8341 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca 8401 ctcccaacga aGataagatc tgctttttgc ttgtactggg tctctctggt tagaccagat 8461 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt 8521 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc 8581 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat 8641 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat 8701 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt 8761 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg 8821 gctctagcta TCCCGCCCCT AACTCCGCCC Atcccgcccc taactccgcc cagttccgcc 8881 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg 8941 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctaga cttttgcaga 9001 gaccaaattc gtaatcatgt catagctgtt tcctgtgtga aattgttatc cgctcacaat 9061 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag 9121 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg 9181 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc 9241 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 9301 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 9361 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 9421 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 9481 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 9541 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 9601 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 9661 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 9721 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 9781 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 9841 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac 9901 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 9961 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 10021 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 10081 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 10141 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 10201 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 10261 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 10321 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 10381 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 10441 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 10501 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 10561 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 10621 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 10681 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 10741 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 10801 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 10861 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 10921 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 10981 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 11041 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 11101 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 11161 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg 11221 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat 11281 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg 11341 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga 11401 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag 11461 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc 11521 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt 11581 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa 11641 gctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.